NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.857	0.000	0.000	-0.080	0.000	0.000
Slide	0.000	2.539	1.003	0.000	-0.063	-0.158
Rise	0.000	1.003	9.919	0.000	-0.040	-0.188
Tilt	-0.080	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.063	-0.040	0.000	0.024	0.011
Twist	0.000	-0.158	-0.188	0.000	0.011	0.034

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.239	0.000	0.000	-0.044	0.000	0.000
Slide	0.000	2.423	1.261	0.000	-0.095	-0.139
Rise	0.000	1.261	7.479	0.000	-0.083	-0.201
Tilt	-0.044	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.095	-0.083	0.000	0.024	0.013
Twist	0.000	-0.139	-0.201	0.000	0.013	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.295	0.000	0.000	-0.105	0.000	0.000
Slide	0.000	2.875	0.772	0.000	-0.030	-0.189
Rise	0.000	0.772	8.546	0.000	-0.061	-0.153
Tilt	-0.105	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.030	-0.061	0.000	0.021	0.005
Twist	0.000	-0.189	-0.153	0.000	0.005	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.881	0.000	0.000	-0.052	0.000	0.000
Slide	0.000	3.399	2.648	0.000	0.022	-0.038
Rise	0.000	2.648	10.839	0.000	0.068	-0.201
Tilt	-0.052	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.022	0.068	0.000	0.025	0.009
Twist	0.000	-0.038	-0.201	0.000	0.009	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.760	0.000	0.000	-0.047	0.000	0.000
Slide	0.000	1.863	1.652	0.000	-0.028	-0.076
Rise	0.000	1.652	7.135	0.000	-0.027	-0.162
Tilt	-0.047	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.028	-0.027	0.000	0.016	0.004
Twist	0.000	-0.076	-0.162	0.000	0.004	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.705	0.000	0.000	-0.076	0.000	0.000
Slide	0.000	3.016	1.028	0.000	0.014	-0.140
Rise	0.000	1.028	7.751	0.000	-0.077	-0.211
Tilt	-0.076	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.014	-0.077	0.000	0.017	0.009
Twist	0.000	-0.140	-0.211	0.000	0.009	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.054	0.000	0.000	-0.137	0.000	0.000
Slide	0.000	3.031	0.858	0.000	-0.007	-0.116
Rise	0.000	0.858	7.468	0.000	-0.080	-0.201
Tilt	-0.137	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.007	-0.080	0.000	0.015	0.004
Twist	0.000	-0.116	-0.201	0.000	0.004	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.930	0.000	0.000	-0.062	0.000	0.000
Slide	0.000	1.949	1.158	0.000	-0.016	-0.105
Rise	0.000	1.158	7.119	0.000	-0.038	-0.172
Tilt	-0.062	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.016	-0.038	0.000	0.020	0.006
Twist	0.000	-0.105	-0.172	0.000	0.006	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.381	0.000	0.000	-0.120	0.000	0.000
Slide	0.000	3.104	3.066	0.000	0.057	-0.039
Rise	0.000	3.066	11.198	0.000	0.130	-0.189
Tilt	-0.120	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.057	0.130	0.000	0.027	0.008
Twist	0.000	-0.039	-0.189	0.000	0.008	0.035

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.4.cte	0.000	0.000

	Mean	Stdev
-aatt.7.cte	0.000	0.000

	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-acgt.9.cte	0.000	0.000

	Mean	Stdev
-agct.5.cte	0.000	0.000

	Mean	Stdev
-catg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.10.cte	0.000	0.000

	Mean	Stdev
-gatc.6.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
rise-10-CGCG	3.301	0.317

	Mean	Stdev
roll-10-CGCG	5.025	6.481

	Mean	Stdev
shift-10-CGCG	0.000	1.117

	Mean	Stdev
slide-10-CGCG	0.240	0.481

	Mean	Stdev
tilt-10-CGCG	0.000	6.390

	Mean	Stdev
twist-10-CGCG	36.722	5.540

	Mean	Stdev
rise-2-GCGC	3.426	0.289

	Mean	Stdev
roll-2-GCGC	-1.141	5.127

	Mean	Stdev
shift-2-GCGC	0.000	0.754

	Mean	Stdev
slide-2-GCGC	-0.473	0.511

	Mean	Stdev
tilt-2-GCGC	0.000	4.368

	Mean	Stdev
twist-2-GCGC	35.565	4.572

	Mean	Stdev
rise-3-CATG	3.187	0.315

	Mean	Stdev
roll-3-CATG	7.117	7.306

	Mean	Stdev
shift-3-CATG	0.000	1.073

	Mean	Stdev
slide-3-CATG	-0.021	0.560

	Mean	Stdev
tilt-3-CATG	0.000	5.279

	Mean	Stdev
twist-3-CATG	31.493	7.590

	Mean	Stdev
rise-4-AATT	3.194	0.258

	Mean	Stdev
roll-4-AATT	1.146	5.476

	Mean	Stdev
shift-4-AATT	0.000	0.965

	Mean	Stdev
slide-4-AATT	-0.015	0.571

	Mean	Stdev
tilt-4-AATT	0.000	5.305

	Mean	Stdev
twist-4-AATT	34.814	5.413

	Mean	Stdev
rise-5-AGCT	3.386	0.335

	Mean	Stdev
roll-5-AGCT	2.511	6.252

	Mean	Stdev
shift-5-AGCT	0.000	0.922

	Mean	Stdev
slide-5-AGCT	-0.581	0.649

	Mean	Stdev
tilt-5-AGCT	0.000	4.510

	Mean	Stdev
twist-5-AGCT	33.516	5.558

	Mean	Stdev
rise-6-GATC	3.411	0.319

	Mean	Stdev
roll-6-GATC	1.255	5.736

	Mean	Stdev
shift-6-GATC	0.000	0.855

	Mean	Stdev
slide-6-GATC	-0.148	0.638

	Mean	Stdev
tilt-6-GATC	0.000	4.786

	Mean	Stdev
twist-6-GATC	36.281	6.386

	Mean	Stdev
rise-7-AATT	3.283	0.311

	Mean	Stdev
roll-7-AATT	2.338	5.759

	Mean	Stdev
shift-7-AATT	0.000	0.705

	Mean	Stdev
slide-7-AATT	-0.297	0.587

	Mean	Stdev
tilt-7-AATT	0.000	4.253

	Mean	Stdev
twist-7-AATT	32.900	5.774

	Mean	Stdev
rise-8-AATT	3.339	0.272

	Mean	Stdev
roll-8-AATT	-0.128	5.360

	Mean	Stdev
shift-8-AATT	0.000	0.793

	Mean	Stdev
slide-8-AATT	-0.255	0.540

	Mean	Stdev
tilt-8-AATT	0.000	5.187

	Mean	Stdev
twist-8-AATT	38.015	4.617

	Mean	Stdev
rise-9-ACGT	3.351	0.280

	Mean	Stdev
roll-9-ACGT	-1.343	5.274

	Mean	Stdev
shift-9-ACGT	0.000	0.847

	Mean	Stdev
slide-9-ACGT	-0.638	0.462

	Mean	Stdev
tilt-9-ACGT	0.000	3.816

	Mean	Stdev
twist-9-ACGT	32.178	4.718

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