NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.930	0.000	0.000	-0.070	0.000	0.000
Slide	0.000	2.900	0.757	0.000	-0.075	-0.140
Rise	0.000	0.757	9.169	0.000	-0.025	-0.202
Tilt	-0.070	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.075	-0.025	0.000	0.023	0.012
Twist	0.000	-0.140	-0.202	0.000	0.012	0.027

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.097	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	3.173	1.205	0.000	-0.076	-0.194
Rise	0.000	1.205	9.078	0.000	-0.040	-0.198
Tilt	-0.095	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.076	-0.040	0.000	0.026	0.012
Twist	0.000	-0.194	-0.198	0.000	0.012	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.895	0.000	0.000	-0.110	0.000	0.000
Slide	0.000	4.504	1.234	0.000	-0.021	-0.344
Rise	0.000	1.234	8.483	0.000	-0.065	-0.145
Tilt	-0.110	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.021	-0.065	0.000	0.026	0.005
Twist	0.000	-0.344	-0.145	0.000	0.005	0.063

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.801	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	2.617	1.090	0.000	-0.087	-0.150
Rise	0.000	1.090	9.175	0.000	-0.037	-0.186
Tilt	-0.048	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.087	-0.037	0.000	0.023	0.012
Twist	0.000	-0.150	-0.186	0.000	0.012	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.442	0.000	0.000	-0.085	0.000	0.000
Slide	0.000	3.109	0.880	0.000	-0.051	-0.173
Rise	0.000	0.880	8.693	0.000	-0.028	-0.160
Tilt	-0.085	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.051	-0.028	0.000	0.026	0.011
Twist	0.000	-0.173	-0.160	0.000	0.011	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.087	0.000	0.000	-0.139	0.000	0.000
Slide	0.000	3.602	1.670	0.000	-0.084	-0.201
Rise	0.000	1.670	8.464	0.000	-0.079	-0.168
Tilt	-0.139	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.084	-0.079	0.000	0.027	0.006
Twist	0.000	-0.201	-0.168	0.000	0.006	0.044

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.894	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	2.321	1.908	0.000	-0.023	-0.097
Rise	0.000	1.908	6.065	0.000	-0.055	-0.184
Tilt	-0.055	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.023	-0.055	0.000	0.018	0.002
Twist	0.000	-0.097	-0.184	0.000	0.002	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.780	0.000	0.000	-0.004	0.000	0.000
Slide	0.000	5.738	2.735	0.000	-0.001	-0.230
Rise	0.000	2.735	11.035	0.000	0.062	-0.163
Tilt	-0.004	0.000	0.000	0.051	0.000	0.000
Roll	0.000	-0.001	0.062	0.000	0.038	0.005
Twist	0.000	-0.230	-0.163	0.000	0.005	0.079

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.725	0.000	0.000	-0.064	0.000	0.000
Slide	0.000	2.595	0.852	0.000	0.015	-0.106
Rise	0.000	0.852	7.905	0.000	-0.108	-0.229
Tilt	-0.064	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.015	-0.108	0.000	0.020	0.011
Twist	0.000	-0.106	-0.229	0.000	0.011	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.784	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	2.410	1.012	0.000	0.018	-0.089
Rise	0.000	1.012	7.470	0.000	-0.100	-0.230
Tilt	-0.059	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.018	-0.100	0.000	0.019	0.009
Twist	0.000	-0.089	-0.230	0.000	0.009	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.138	0.000	0.000	-0.119	0.000	0.000
Slide	0.000	3.381	0.418	0.000	0.025	-0.114
Rise	0.000	0.418	8.825	0.000	-0.082	-0.269
Tilt	-0.119	0.000	0.000	0.034	0.000	0.000
Roll	0.000	0.025	-0.082	0.000	0.020	0.007
Twist	0.000	-0.114	-0.269	0.000	0.007	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.338	0.000	0.000	-0.140	0.000	0.000
Slide	0.000	3.120	2.733	0.000	0.067	-0.077
Rise	0.000	2.733	12.319	0.000	0.152	-0.261
Tilt	-0.140	0.000	0.000	0.047	0.000	0.000
Roll	0.000	0.067	0.152	0.000	0.028	0.004
Twist	0.000	-0.077	-0.261	0.000	0.004	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.359	0.000	0.000	-0.132	0.000	0.000
Slide	0.000	3.242	2.721	0.000	0.073	-0.113
Rise	0.000	2.721	11.712	0.000	0.146	-0.256
Tilt	-0.132	0.000	0.000	0.046	0.000	0.000
Roll	0.000	0.073	0.146	0.000	0.026	0.006
Twist	0.000	-0.113	-0.256	0.000	0.006	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.439	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	2.599	1.104	0.000	-0.013	-0.167
Rise	0.000	1.104	8.051	0.000	-0.095	-0.219
Tilt	-0.048	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.013	-0.095	0.000	0.017	0.013
Twist	0.000	-0.167	-0.219	0.000	0.013	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.722	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	2.832	1.001	0.000	-0.050	-0.149
Rise	0.000	1.001	9.640	0.000	-0.007	-0.154
Tilt	-0.094	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.050	-0.007	0.000	0.022	0.009
Twist	0.000	-0.149	-0.154	0.000	0.009	0.046

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.15.cte	0.000	0.000

	Mean	Stdev
-aatt.3.cte	0.000	0.000

	Mean	Stdev
-aatt.4.cte	0.000	0.000

	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-aatt.9.cte	0.000	0.000

	Mean	Stdev
-agct.10.cte	0.000	0.000

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-atat.5.cte	0.000	0.000

	Mean	Stdev
-catg.14.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.12.cte	0.000	0.000

	Mean	Stdev
-gcgc.11.cte	0.000	0.000

	Mean	Stdev
-gcgc.13.cte	0.000	0.000

	Mean	Stdev
-tata.7.cte	0.000	0.000

	Mean	Stdev
-ttaa.6.cte	0.000	0.000

	Mean	Stdev
rise-10-AGCT	3.473	0.280

	Mean	Stdev
roll-10-AGCT	2.959	4.840

	Mean	Stdev
shift-10-AGCT	0.000	0.616

	Mean	Stdev
slide-10-AGCT	-0.842	0.486

	Mean	Stdev
tilt-10-AGCT	0.000	4.731

	Mean	Stdev
twist-10-AGCT	36.083	4.265

	Mean	Stdev
rise-11-GCGC	3.445	0.264

	Mean	Stdev
roll-11-GCGC	-0.531	4.916

	Mean	Stdev
shift-11-GCGC	0.000	0.797

	Mean	Stdev
slide-11-GCGC	-0.342	0.489

	Mean	Stdev
tilt-11-GCGC	0.000	4.239

	Mean	Stdev
twist-11-GCGC	36.416	4.160

	Mean	Stdev
rise-12-CGCG	3.027	0.334

	Mean	Stdev
roll-12-CGCG	6.396	5.873

	Mean	Stdev
shift-12-CGCG	0.000	0.898

	Mean	Stdev
slide-12-CGCG	-0.085	0.489

	Mean	Stdev
tilt-12-CGCG	0.000	5.182

	Mean	Stdev
twist-12-CGCG	30.042	7.698

	Mean	Stdev
rise-13-GCGC	3.417	0.272

	Mean	Stdev
roll-13-GCGC	-1.932	5.434

	Mean	Stdev
shift-13-GCGC	0.000	0.773

	Mean	Stdev
slide-13-GCGC	-0.207	0.497

	Mean	Stdev
tilt-13-GCGC	0.000	4.197

	Mean	Stdev
twist-13-GCGC	36.067	4.757

	Mean	Stdev
rise-14-CATG	3.174	0.324

	Mean	Stdev
roll-14-CATG	7.638	6.954

	Mean	Stdev
shift-14-CATG	0.000	1.010

	Mean	Stdev
slide-14-CATG	-0.088	0.576

	Mean	Stdev
tilt-14-CATG	0.000	5.240

	Mean	Stdev
twist-14-CATG	31.795	8.055

	Mean	Stdev
rise-15-AATT	3.204	0.281

	Mean	Stdev
roll-15-AATT	1.472	5.817

	Mean	Stdev
shift-15-AATT	0.000	0.883

	Mean	Stdev
slide-15-AATT	-0.029	0.521

	Mean	Stdev
tilt-15-AATT	0.000	5.149

	Mean	Stdev
twist-15-AATT	34.807	6.393

	Mean	Stdev
rise-16-AGCT	3.405	0.382

	Mean	Stdev
roll-16-AGCT	2.246	5.860

	Mean	Stdev
shift-16-AGCT	0.000	0.858

	Mean	Stdev
slide-16-AGCT	-0.533	0.595

	Mean	Stdev
tilt-16-AGCT	0.000	4.660

	Mean	Stdev
twist-16-AGCT	34.051	5.004

	Mean	Stdev
rise-2-CATG	3.223	0.342

	Mean	Stdev
roll-2-CATG	8.066	6.628

	Mean	Stdev
shift-2-CATG	0.000	0.947

	Mean	Stdev
slide-2-CATG	-0.111	0.566

	Mean	Stdev
tilt-2-CATG	0.000	5.134

	Mean	Stdev
twist-2-CATG	31.896	7.614

	Mean	Stdev
rise-3-AATT	3.318	0.273

	Mean	Stdev
roll-3-AATT	0.983	5.226

	Mean	Stdev
shift-3-AATT	0.000	0.828

	Mean	Stdev
slide-3-AATT	-0.171	0.539

	Mean	Stdev
tilt-3-AATT	0.000	4.511

	Mean	Stdev
twist-3-AATT	35.432	5.861

	Mean	Stdev
rise-4-AATT	3.396	0.271

	Mean	Stdev
roll-4-AATT	0.230	4.786

	Mean	Stdev
shift-4-AATT	0.000	0.622

	Mean	Stdev
slide-4-AATT	-0.433	0.475

	Mean	Stdev
tilt-4-AATT	0.000	4.817

	Mean	Stdev
twist-4-AATT	38.324	4.024

	Mean	Stdev
rise-5-ATAT	3.366	0.247

	Mean	Stdev
roll-5-ATAT	-2.694	3.972

	Mean	Stdev
shift-5-ATAT	0.000	0.459

	Mean	Stdev
slide-5-ATAT	-0.943	0.357

	Mean	Stdev
tilt-5-ATAT	0.000	3.386

	Mean	Stdev
twist-5-ATAT	32.829	2.925

	Mean	Stdev
rise-6-TTAA	3.259	0.255

	Mean	Stdev
roll-6-TTAA	-0.154	5.389

	Mean	Stdev
shift-6-TTAA	0.000	1.124

	Mean	Stdev
slide-6-TTAA	-0.033	0.505

	Mean	Stdev
tilt-6-TTAA	0.000	5.137

	Mean	Stdev
twist-6-TTAA	37.275	4.060

	Mean	Stdev
rise-7-TATA	3.147	0.302

	Mean	Stdev
roll-7-TATA	6.985	7.993

	Mean	Stdev
shift-7-TATA	0.000	1.289

	Mean	Stdev
slide-7-TATA	0.075	0.660

	Mean	Stdev
tilt-7-TATA	0.000	5.221

	Mean	Stdev
twist-7-TATA	31.310	7.919

	Mean	Stdev
rise-8-AATT	3.205	0.271

	Mean	Stdev
roll-8-AATT	0.681	5.811

	Mean	Stdev
shift-8-AATT	0.000	0.895

	Mean	Stdev
slide-8-AATT	-0.129	0.565

	Mean	Stdev
tilt-8-AATT	0.000	4.364

	Mean	Stdev
twist-8-AATT	34.051	5.661

	Mean	Stdev
rise-9-AATT	3.330	0.270

	Mean	Stdev
roll-9-AATT	-0.323	5.065

	Mean	Stdev
shift-9-AATT	0.000	0.695

	Mean	Stdev
slide-9-AATT	-0.282	0.500

	Mean	Stdev
tilt-9-AATT	0.000	4.680

	Mean	Stdev
twist-9-AATT	36.844	4.738

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