NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.746	0.000	0.000	-0.107	0.000	0.000
Slide	0.000	2.704	0.815	0.000	-0.077	-0.147
Rise	0.000	0.815	9.375	0.000	-0.034	-0.169
Tilt	-0.107	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.077	-0.034	0.000	0.022	0.010
Twist	0.000	-0.147	-0.169	0.000	0.010	0.039

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.052	0.000	0.000	-0.110	0.000	0.000
Slide	0.000	2.754	1.320	0.000	-0.057	-0.190
Rise	0.000	1.320	8.898	0.000	-0.027	-0.194
Tilt	-0.110	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.057	-0.027	0.000	0.024	0.008
Twist	0.000	-0.190	-0.194	0.000	0.008	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.760	0.000	0.000	-0.064	0.000	0.000
Slide	0.000	2.607	1.219	0.000	-0.085	-0.157
Rise	0.000	1.219	8.680	0.000	-0.036	-0.169
Tilt	-0.064	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.085	-0.036	0.000	0.024	0.011
Twist	0.000	-0.157	-0.169	0.000	0.011	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.795	0.000	0.000	-0.069	0.000	0.000
Slide	0.000	3.163	1.460	0.000	-0.060	-0.170
Rise	0.000	1.460	8.036	0.000	-0.055	-0.161
Tilt	-0.069	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.060	-0.055	0.000	0.025	0.008
Twist	0.000	-0.170	-0.161	0.000	0.008	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.065	0.000	0.000	-0.104	0.000	0.000
Slide	0.000	3.929	1.085	0.000	-0.051	-0.308
Rise	0.000	1.085	8.160	0.000	-0.045	-0.143
Tilt	-0.104	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.051	-0.045	0.000	0.026	0.006
Twist	0.000	-0.308	-0.143	0.000	0.006	0.058

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.893	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	3.653	3.192	0.000	0.020	-0.076
Rise	0.000	3.192	11.198	0.000	0.068	-0.208
Tilt	-0.043	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.020	0.068	0.000	0.027	0.010
Twist	0.000	-0.076	-0.208	0.000	0.010	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.888	0.000	0.000	-0.023	0.000	0.000
Slide	0.000	5.348	2.768	0.000	-0.030	-0.176
Rise	0.000	2.768	10.456	0.000	0.025	-0.191
Tilt	-0.023	0.000	0.000	0.049	0.000	0.000
Roll	0.000	-0.030	0.025	0.000	0.033	0.011
Twist	0.000	-0.176	-0.191	0.000	0.011	0.064

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.497	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	5.082	3.001	0.000	-0.013	-0.178
Rise	0.000	3.001	11.721	0.000	0.068	-0.147
Tilt	-0.002	0.000	0.000	0.048	0.000	0.000
Roll	0.000	-0.013	0.068	0.000	0.032	0.010
Twist	0.000	-0.178	-0.147	0.000	0.010	0.063

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.799	0.000	0.000	-0.065	0.000	0.000
Slide	0.000	2.253	1.580	0.000	0.017	-0.093
Rise	0.000	1.580	7.231	0.000	-0.105	-0.243
Tilt	-0.065	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.017	-0.105	0.000	0.019	0.009
Twist	0.000	-0.093	-0.243	0.000	0.009	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.520	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.557	1.572	0.000	-0.053	-0.107
Rise	0.000	1.572	7.234	0.000	-0.039	-0.128
Tilt	-0.088	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.053	-0.039	0.000	0.023	0.005
Twist	0.000	-0.107	-0.128	0.000	0.005	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.487	0.000	0.000	-0.057	0.000	0.000
Slide	0.000	2.325	0.843	0.000	0.031	-0.126
Rise	0.000	0.843	7.634	0.000	-0.096	-0.214
Tilt	-0.057	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.031	-0.096	0.000	0.017	0.010
Twist	0.000	-0.126	-0.214	0.000	0.010	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.403	0.000	0.000	-0.042	0.000	0.000
Slide	0.000	2.488	0.826	0.000	0.006	-0.142
Rise	0.000	0.826	8.459	0.000	-0.133	-0.227
Tilt	-0.042	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.006	-0.133	0.000	0.020	0.014
Twist	0.000	-0.142	-0.227	0.000	0.014	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.428	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	2.467	1.309	0.000	0.026	-0.119
Rise	0.000	1.309	7.797	0.000	-0.109	-0.236
Tilt	-0.061	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.026	-0.109	0.000	0.018	0.012
Twist	0.000	-0.119	-0.236	0.000	0.012	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.731	0.000	0.000	-0.071	0.000	0.000
Slide	0.000	2.827	0.871	0.000	-0.061	-0.142
Rise	0.000	0.871	9.675	0.000	-0.001	-0.186
Tilt	-0.071	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.061	-0.001	0.000	0.022	0.011
Twist	0.000	-0.142	-0.186	0.000	0.011	0.035

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.13.cte	0.000	0.000

	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-aatt.7.cte	0.000	0.000

	Mean	Stdev
-acgt.11.cte	0.000	0.000

	Mean	Stdev
-atat.14.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-catg.12.cte	0.000	0.000

	Mean	Stdev
-ctag.2.cte	0.000	0.000

	Mean	Stdev
-tata.15.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
-ttaa.3.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.225	0.261

	Mean	Stdev
roll-10-AATT	1.000	5.555

	Mean	Stdev
shift-10-AATT	0.000	1.197

	Mean	Stdev
slide-10-AATT	0.079	0.532

	Mean	Stdev
tilt-10-AATT	0.000	5.616

	Mean	Stdev
twist-10-AATT	36.086	4.636

	Mean	Stdev
rise-11-ACGT	3.366	0.279

	Mean	Stdev
roll-11-ACGT	-1.196	5.010

	Mean	Stdev
shift-11-ACGT	0.000	0.832

	Mean	Stdev
slide-11-ACGT	-0.687	0.463

	Mean	Stdev
tilt-11-ACGT	0.000	3.919

	Mean	Stdev
twist-11-ACGT	32.717	4.364

	Mean	Stdev
rise-12-CATG	3.357	0.365

	Mean	Stdev
roll-12-CATG	7.981	6.749

	Mean	Stdev
shift-12-CATG	0.000	0.960

	Mean	Stdev
slide-12-CATG	-0.201	0.611

	Mean	Stdev
tilt-12-CATG	0.000	5.340

	Mean	Stdev
twist-12-CATG	33.935	7.474

	Mean	Stdev
rise-13-AATT	3.340	0.272

	Mean	Stdev
roll-13-AATT	0.258	5.094

	Mean	Stdev
shift-13-AATT	0.000	0.934

	Mean	Stdev
slide-13-AATT	-0.189	0.553

	Mean	Stdev
tilt-13-AATT	0.000	5.381

	Mean	Stdev
twist-13-AATT	37.363	4.339

	Mean	Stdev
rise-14-ATAT	3.369	0.259

	Mean	Stdev
roll-14-ATAT	-2.543	4.353

	Mean	Stdev
shift-14-ATAT	0.000	0.559

	Mean	Stdev
slide-14-ATAT	-0.856	0.367

	Mean	Stdev
tilt-14-ATAT	0.000	3.477

	Mean	Stdev
twist-14-ATAT	32.903	3.292

	Mean	Stdev
rise-15-TATA	3.289	0.306

	Mean	Stdev
roll-15-TATA	2.946	6.981

	Mean	Stdev
shift-15-TATA	0.000	1.255

	Mean	Stdev
slide-15-TATA	0.235	0.607

	Mean	Stdev
tilt-15-TATA	0.000	5.252

	Mean	Stdev
twist-15-TATA	38.654	5.275

	Mean	Stdev
rise-2-CTAG	3.469	0.310

	Mean	Stdev
roll-2-CTAG	2.088	5.159

	Mean	Stdev
shift-2-CTAG	0.000	0.677

	Mean	Stdev
slide-2-CTAG	-0.753	0.549

	Mean	Stdev
tilt-2-CTAG	0.000	4.647

	Mean	Stdev
twist-2-CTAG	35.018	4.934

	Mean	Stdev
rise-3-TTAA	3.220	0.263

	Mean	Stdev
roll-3-TTAA	-0.226	5.717

	Mean	Stdev
shift-3-TTAA	0.000	1.021

	Mean	Stdev
slide-3-TTAA	-0.001	0.513

	Mean	Stdev
tilt-3-TTAA	0.000	5.046

	Mean	Stdev
twist-3-TTAA	35.587	5.153

	Mean	Stdev
rise-4-TATA	3.117	0.297

	Mean	Stdev
roll-4-TATA	7.585	7.681

	Mean	Stdev
shift-4-TATA	0.000	1.325

	Mean	Stdev
slide-4-TATA	0.170	0.659

	Mean	Stdev
tilt-4-TATA	0.000	5.220

	Mean	Stdev
twist-4-TATA	31.129	7.882

	Mean	Stdev
rise-5-AATT	3.254	0.276

	Mean	Stdev
roll-5-AATT	1.206	5.552

	Mean	Stdev
shift-5-AATT	0.000	0.959

	Mean	Stdev
slide-5-AATT	-0.092	0.572

	Mean	Stdev
tilt-5-AATT	0.000	4.570

	Mean	Stdev
twist-5-AATT	35.292	5.324

	Mean	Stdev
rise-6-AATT	3.380	0.288

	Mean	Stdev
roll-6-AATT	0.791	5.088

	Mean	Stdev
shift-6-AATT	0.000	0.594

	Mean	Stdev
slide-6-AATT	-0.463	0.515

	Mean	Stdev
tilt-6-AATT	0.000	4.232

	Mean	Stdev
twist-6-AATT	35.277	5.268

	Mean	Stdev
rise-7-AATT	3.372	0.275

	Mean	Stdev
roll-7-AATT	0.137	4.788

	Mean	Stdev
shift-7-AATT	0.000	0.586

	Mean	Stdev
slide-7-AATT	-0.560	0.508

	Mean	Stdev
tilt-7-AATT	0.000	4.820

	Mean	Stdev
twist-7-AATT	37.232	4.177

	Mean	Stdev
rise-8-ATAT	3.305	0.246

	Mean	Stdev
roll-8-ATAT	-2.568	4.430

	Mean	Stdev
shift-8-ATAT	0.000	0.626

	Mean	Stdev
slide-8-ATAT	-0.888	0.383

	Mean	Stdev
tilt-8-ATAT	0.000	3.507

	Mean	Stdev
twist-8-ATAT	32.450	3.310

	Mean	Stdev
rise-9-TATA	3.225	0.316

	Mean	Stdev
roll-9-TATA	4.757	7.435

	Mean	Stdev
shift-9-TATA	0.000	1.442

	Mean	Stdev
slide-9-TATA	0.119	0.606

	Mean	Stdev
tilt-9-TATA	0.000	5.866

	Mean	Stdev
twist-9-TATA	34.970	6.236

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