NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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3'	G	-	T	-	G	-	G	-	G	-	A	-	A	-	A	-	C	-	T	-	T	-	C	-	G	-	A	-	G	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.211	0.000	0.000	-0.024	0.000	0.000
Slide	0.000	2.797	0.776	0.000	-0.100	-0.122
Rise	0.000	0.776	8.851	0.000	-0.044	-0.169
Tilt	-0.024	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.100	-0.044	0.000	0.025	0.011
Twist	0.000	-0.122	-0.169	0.000	0.011	0.027

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.010	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	3.659	2.761	0.000	0.027	-0.099
Rise	0.000	2.761	9.514	0.000	0.146	-0.124
Tilt	-0.048	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.027	0.146	0.000	0.021	0.001
Twist	0.000	-0.099	-0.124	0.000	0.001	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.443	0.000	0.000	-0.134	0.000	0.000
Slide	0.000	2.757	1.602	0.000	-0.055	-0.151
Rise	0.000	1.602	8.223	0.000	-0.042	-0.156
Tilt	-0.134	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.055	-0.042	0.000	0.024	0.003
Twist	0.000	-0.151	-0.156	0.000	0.003	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.239	0.000	0.000	-0.112	0.000	0.000
Slide	0.000	1.462	1.775	0.000	0.002	-0.083
Rise	0.000	1.775	7.626	0.000	-0.032	-0.192
Tilt	-0.112	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.002	-0.032	0.000	0.020	0.005
Twist	0.000	-0.083	-0.192	0.000	0.005	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.255	0.000	0.000	-0.108	0.000	0.000
Slide	0.000	1.519	1.780	0.000	0.001	-0.086
Rise	0.000	1.780	8.083	0.000	-0.029	-0.188
Tilt	-0.108	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.001	-0.029	0.000	0.021	0.004
Twist	0.000	-0.086	-0.188	0.000	0.004	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.374	0.000	0.000	-0.102	0.000	0.000
Slide	0.000	2.743	1.756	0.000	-0.053	-0.125
Rise	0.000	1.756	7.850	0.000	-0.065	-0.173
Tilt	-0.102	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.053	-0.065	0.000	0.023	0.005
Twist	0.000	-0.125	-0.173	0.000	0.005	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.154	0.000	0.000	-0.063	0.000	0.000
Slide	0.000	2.083	1.645	0.000	-0.055	-0.068
Rise	0.000	1.645	7.973	0.000	-0.047	-0.176
Tilt	-0.063	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.055	-0.047	0.000	0.021	0.004
Twist	0.000	-0.068	-0.176	0.000	0.004	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.773	0.000	0.000	-0.080	0.000	0.000
Slide	0.000	2.496	1.291	0.000	-0.054	-0.132
Rise	0.000	1.291	8.633	0.000	-0.037	-0.158
Tilt	-0.080	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.054	-0.037	0.000	0.022	0.011
Twist	0.000	-0.132	-0.158	0.000	0.011	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.472	0.000	0.000	-0.130	0.000	0.000
Slide	0.000	3.391	2.739	0.000	0.059	-0.166
Rise	0.000	2.739	11.988	0.000	0.147	-0.201
Tilt	-0.130	0.000	0.000	0.046	0.000	0.000
Roll	0.000	0.059	0.147	0.000	0.034	0.003
Twist	0.000	-0.166	-0.201	0.000	0.003	0.045

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.755	0.000	0.000	-0.064	0.000	0.000
Slide	0.000	2.602	0.907	0.000	0.007	-0.113
Rise	0.000	0.907	8.168	0.000	-0.139	-0.261
Tilt	-0.064	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.007	-0.139	0.000	0.020	0.011
Twist	0.000	-0.113	-0.261	0.000	0.011	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.177	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	2.772	1.027	0.000	-0.067	-0.127
Rise	0.000	1.027	8.781	0.000	-0.054	-0.159
Tilt	-0.055	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.067	-0.054	0.000	0.021	0.010
Twist	0.000	-0.127	-0.159	0.000	0.010	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.845	0.000	0.000	-0.069	0.000	0.000
Slide	0.000	2.737	1.250	0.000	-0.056	-0.175
Rise	0.000	1.250	9.095	0.000	-0.010	-0.184
Tilt	-0.069	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.056	-0.010	0.000	0.020	0.008
Twist	0.000	-0.175	-0.184	0.000	0.008	0.028

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-acgt.2.cte	0.000	0.000

	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-ccgg.3.cte	0.000	0.000

	Mean	Stdev
-ccgg.4.cte	0.000	0.000

	Mean	Stdev
-ctag.13.cte	0.000	0.000

	Mean	Stdev
-ctag.5.cte	0.000	0.000

	Mean	Stdev
-gatc.9.cte	0.000	0.000

	Mean	Stdev
-gcgc.12.cte	0.000	0.000

	Mean	Stdev
-tgca.8.cte	0.000	0.000

	Mean	Stdev
-ttaa.6.cte	0.000	0.000

	Mean	Stdev
-ttaa.7.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.210	0.275

	Mean	Stdev
roll-10-AATT	1.127	5.602

	Mean	Stdev
shift-10-AATT	0.000	0.701

	Mean	Stdev
slide-10-AATT	-0.361	0.525

	Mean	Stdev
tilt-10-AATT	0.000	4.563

	Mean	Stdev
twist-10-AATT	33.089	5.788

	Mean	Stdev
rise-11-AGCT	3.452	0.287

	Mean	Stdev
roll-11-AGCT	2.892	5.015

	Mean	Stdev
shift-11-AGCT	0.000	0.787

	Mean	Stdev
slide-11-AGCT	-0.641	0.554

	Mean	Stdev
tilt-11-AGCT	0.000	4.964

	Mean	Stdev
twist-11-AGCT	35.941	4.876

	Mean	Stdev
rise-12-GCGC	3.574	0.251

	Mean	Stdev
roll-12-GCGC	-0.123	4.367

	Mean	Stdev
shift-12-GCGC	0.000	0.527

	Mean	Stdev
slide-12-GCGC	-0.833	0.497

	Mean	Stdev
tilt-12-GCGC	0.000	3.850

	Mean	Stdev
twist-12-GCGC	37.142	4.055

	Mean	Stdev
rise-13-CTAG	3.406	0.304

	Mean	Stdev
roll-13-CTAG	2.501	5.181

	Mean	Stdev
shift-13-CTAG	0.000	0.740

	Mean	Stdev
slide-13-CTAG	-0.552	0.537

	Mean	Stdev
tilt-13-CTAG	0.000	4.677

	Mean	Stdev
twist-13-CTAG	34.451	5.061

	Mean	Stdev
rise-2-ACGT	3.281	0.301

	Mean	Stdev
roll-2-ACGT	-1.075	5.590

	Mean	Stdev
shift-2-ACGT	0.000	0.785

	Mean	Stdev
slide-2-ACGT	-0.491	0.474

	Mean	Stdev
tilt-2-ACGT	0.000	4.026

	Mean	Stdev
twist-2-ACGT	32.836	6.417

	Mean	Stdev
rise-3-CCGG	3.501	0.338

	Mean	Stdev
roll-3-CCGG	3.352	5.520

	Mean	Stdev
shift-3-CCGG	0.000	0.801

	Mean	Stdev
slide-3-CCGG	-0.722	0.775

	Mean	Stdev
tilt-3-CCGG	0.000	4.546

	Mean	Stdev
twist-3-CCGG	35.644	5.080

	Mean	Stdev
rise-4-CCGG	3.494	0.321

	Mean	Stdev
roll-4-CCGG	3.577	5.367

	Mean	Stdev
shift-4-CCGG	0.000	0.790

	Mean	Stdev
slide-4-CCGG	-0.706	0.749

	Mean	Stdev
tilt-4-CCGG	0.000	4.597

	Mean	Stdev
twist-4-CCGG	34.780	4.902

	Mean	Stdev
rise-5-CTAG	3.339	0.313

	Mean	Stdev
roll-5-CTAG	2.760	5.478

	Mean	Stdev
shift-5-CTAG	0.000	0.755

	Mean	Stdev
slide-5-CTAG	-0.608	0.603

	Mean	Stdev
tilt-5-CTAG	0.000	4.615

	Mean	Stdev
twist-5-CTAG	33.643	5.536

	Mean	Stdev
rise-6-TTAA	3.267	0.273

	Mean	Stdev
roll-6-TTAA	0.494	5.835

	Mean	Stdev
shift-6-TTAA	0.000	0.743

	Mean	Stdev
slide-6-TTAA	-0.278	0.519

	Mean	Stdev
tilt-6-TTAA	0.000	4.931

	Mean	Stdev
twist-6-TTAA	35.746	5.425

	Mean	Stdev
rise-7-TTAA	3.239	0.274

	Mean	Stdev
roll-7-TTAA	-0.129	5.741

	Mean	Stdev
shift-7-TTAA	0.000	0.911

	Mean	Stdev
slide-7-TTAA	-0.160	0.595

	Mean	Stdev
tilt-7-TTAA	0.000	4.535

	Mean	Stdev
twist-7-TTAA	34.185	6.277

	Mean	Stdev
rise-8-TGCA	3.091	0.335

	Mean	Stdev
roll-8-TGCA	8.447	6.665

	Mean	Stdev
shift-8-TGCA	0.000	0.985

	Mean	Stdev
slide-8-TGCA	0.082	0.567

	Mean	Stdev
tilt-8-TGCA	0.000	5.182

	Mean	Stdev
twist-8-TGCA	29.693	7.830

	Mean	Stdev
rise-9-GATC	3.322	0.278

	Mean	Stdev
roll-9-GATC	2.028	5.756

	Mean	Stdev
shift-9-GATC	0.000	1.000

	Mean	Stdev
slide-9-GATC	0.166	0.560

	Mean	Stdev
tilt-9-GATC	0.000	4.748

	Mean	Stdev
twist-9-GATC	36.479	5.585

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3'	G	-	T	-	G	-	G	-	G	-	A	-	A	-	A	-	C	-	T	-	T	-	C	-	G	-	A	-	G	5'
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