NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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3'	C	-	G	-	A	-	C	-	A	-	G	-	A	-	C	-	C	-	C	-	U	-	G	-	G	-	U	-	C	-	G	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.067	0.000	0.000	-0.001	0.000	0.000
Slide	0.000	4.229	0.690	0.000	-0.001	0.017
Rise	0.000	0.690	6.819	0.000	-0.010	-0.012
Tilt	-0.001	0.000	0.000	0.000	0.000	0.000
Roll	0.000	-0.001	-0.010	0.000	0.000	0.000
Twist	0.000	0.017	-0.012	0.000	0.000	0.001

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.335	0.000	0.000	-0.027	0.000	0.000
Slide	0.000	3.278	-0.430	0.000	0.036	-0.036
Rise	0.000	-0.437	1.045	0.000	0.005	0.053
Tilt	-0.027	0.000	0.000	0.003	0.000	0.000
Roll	0.000	0.036	0.005	0.000	0.001	-0.001
Twist	0.000	-0.037	0.053	0.000	-0.001	0.005

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.504	0.000	0.000	0.004	0.000	0.000
Slide	0.000	3.458	-0.483	0.000	-0.041	-0.016
Rise	0.000	-0.482	3.092	0.000	0.005	0.012
Tilt	0.004	0.000	0.000	0.000	0.000	0.000
Roll	0.000	-0.042	0.005	0.000	0.004	0.002
Twist	0.000	-0.016	0.012	0.000	0.002	0.001

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.476	0.000	0.000	-0.063	0.000	0.000
Slide	0.000	3.189	-0.710	0.000	0.028	-0.009
Rise	0.000	-0.704	1.917	0.000	0.017	0.043
Tilt	-0.063	0.000	0.000	0.009	0.000	0.000
Roll	0.000	0.029	0.018	0.000	0.006	-0.007
Twist	0.000	-0.009	0.042	0.000	-0.007	0.011

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.413	0.000	0.000	-0.066	0.000	0.000
Slide	0.000	5.807	0.519	0.000	-0.006	-0.104
Rise	0.000	0.519	8.362	0.000	-0.215	-0.197
Tilt	-0.066	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.006	-0.215	0.000	0.024	0.000
Twist	0.000	-0.104	-0.197	0.000	0.000	0.075

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.641	0.000	0.000	0.001	0.000	0.000
Slide	0.000	2.791	-0.338	0.000	0.007	-0.000
Rise	0.000	-0.338	6.158	0.000	-0.015	-0.008
Tilt	0.001	0.000	0.000	0.000	0.000	0.000
Roll	0.000	0.007	-0.015	0.000	0.001	-0.000
Twist	0.000	-0.000	-0.008	0.000	-0.000	0.001

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.700	0.000	0.000	-0.008	0.000	0.000
Slide	0.000	5.239	1.162	0.000	0.005	-0.036
Rise	0.000	1.162	10.163	0.000	-0.309	-0.231
Tilt	-0.008	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.005	-0.309	0.000	0.029	0.003
Twist	0.000	-0.036	-0.231	0.000	0.003	0.076

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.567	0.000	0.000	0.003	0.000	0.000
Slide	0.000	3.987	0.760	0.000	-0.024	0.022
Rise	0.000	0.760	2.858	0.000	0.007	0.008
Tilt	0.003	0.000	0.000	0.000	0.000	0.000
Roll	0.000	-0.024	0.007	0.000	0.006	-0.000
Twist	0.000	0.022	0.008	0.000	-0.000	0.000

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.622	0.000	0.000	0.003	0.000	0.000
Slide	0.000	5.578	0.409	0.000	0.014	-0.015
Rise	0.000	0.409	2.953	0.000	0.023	-0.005
Tilt	0.003	0.000	0.000	0.000	0.000	0.000
Roll	0.000	0.014	0.023	0.000	0.007	-0.001
Twist	0.000	-0.015	-0.005	0.000	-0.001	0.000

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.618	0.000	0.000	-0.120	0.000	0.000
Slide	0.000	5.230	0.196	0.000	-0.005	-0.095
Rise	0.000	0.196	12.041	0.000	-0.337	-0.231
Tilt	-0.120	0.000	0.000	0.046	0.000	0.000
Roll	0.000	-0.005	-0.337	0.000	0.034	0.005
Twist	0.000	-0.095	-0.231	0.000	0.005	0.071

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.928	0.000	0.000	-0.098	0.000	0.000
Slide	0.000	6.391	0.137	0.000	0.004	-0.074
Rise	0.000	0.137	11.171	0.000	-0.333	-0.228
Tilt	-0.098	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.004	-0.333	0.000	0.032	0.005
Twist	0.000	-0.074	-0.228	0.000	0.005	0.074

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.767	0.000	0.000	0.005	0.000	0.000
Slide	0.000	4.108	0.808	0.000	0.013	-0.009
Rise	0.000	0.807	3.148	0.000	-0.012	-0.016
Tilt	0.005	0.000	0.000	0.000	0.000	0.000
Roll	0.000	0.013	-0.012	0.000	0.002	0.001
Twist	0.000	-0.009	-0.016	0.000	0.001	0.001

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.678	0.000	0.000	0.015	0.000	0.000
Slide	0.000	6.652	-0.295	0.000	-0.065	0.084
Rise	0.000	-0.295	9.731	0.000	-0.300	-0.143
Tilt	0.015	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.065	-0.300	0.000	0.028	-0.002
Twist	0.000	0.084	-0.143	0.000	-0.002	0.082

[Image courtesy of Curves+]

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	Mean	Stdev
-agag.2.cte	0.000	0.000

	Mean	Stdev
-aggu.11.cte	0.000	0.000

	Mean	Stdev
-ccac.4.cte	0.000	0.000

	Mean	Stdev
-ccga.5.cte	0.000	0.000

	Mean	Stdev
-cuag.6.cte	0.000	0.000

	Mean	Stdev
-cucu.14.cte	0.000	0.000

	Mean	Stdev
-gauc.10.cte	0.000	0.000

	Mean	Stdev
-gcca.3.cte	0.000	0.000

	Mean	Stdev
-gcug.13.cte	0.000	0.000

	Mean	Stdev
-ggcc.8.cte	0.000	0.000

	Mean	Stdev
-ggcc.9.cte	0.000	0.000

	Mean	Stdev
-gggg.12.cte	0.000	0.000

	Mean	Stdev
-ugca.7.cte	0.000	0.000

	Mean	Stdev
rise-10-GAUC	3.215	0.310

	Mean	Stdev
roll-10-GAUC	10.556	5.531

	Mean	Stdev
shift-10-GAUC	0.000	0.916

	Mean	Stdev
slide-10-GAUC	-1.552	0.342

	Mean	Stdev
tilt-10-GAUC	0.000	4.239

	Mean	Stdev
twist-10-GAUC	32.247	2.892

	Mean	Stdev
rise-11-AGGU	3.863	2.137

	Mean	Stdev
roll-11-AGGU	-37.495	55.758

	Mean	Stdev
shift-11-AGGU	0.000	3.169

	Mean	Stdev
slide-11-AGGU	-3.851	0.720

	Mean	Stdev
tilt-11-AGGU	0.000	36.344

	Mean	Stdev
twist-11-AGGU	-60.816	30.902

	Mean	Stdev
rise-12-GGGG	0.550	0.457

	Mean	Stdev
roll-12-GGGG	4.076	47.066

	Mean	Stdev
shift-12-GGGG	0.000	1.044

	Mean	Stdev
slide-12-GGGG	-0.809	0.435

	Mean	Stdev
tilt-12-GGGG	51.139	57.600

	Mean	Stdev
twist-12-GGGG	-7.497	34.725

	Mean	Stdev
rise-13-GCUG	0.761	0.453

	Mean	Stdev
roll-13-GCUG	-0.194	22.947

	Mean	Stdev
shift-13-GCUG	0.000	1.079

	Mean	Stdev
slide-13-GCUG	-0.121	0.340

	Mean	Stdev
tilt-13-GCUG	23.773	57.507

	Mean	Stdev
twist-13-GCUG	14.330	31.775

	Mean	Stdev
rise-14-CUCU	0.324	0.320

	Mean	Stdev
roll-14-CUCU	4.855	21.290

	Mean	Stdev
shift-14-CUCU	0.000	0.598

	Mean	Stdev
slide-14-CUCU	-0.139	0.464

	Mean	Stdev
tilt-14-CUCU	4.950	39.077

	Mean	Stdev
twist-14-CUCU	10.628	29.143

	Mean	Stdev
rise-2-AGAG	0.288	0.311

	Mean	Stdev
roll-2-AGAG	-5.977	38.003

	Mean	Stdev
shift-2-AGAG	0.000	0.534

	Mean	Stdev
slide-2-AGAG	-0.129	0.402

	Mean	Stdev
tilt-2-AGAG	11.196	66.617

	Mean	Stdev
twist-2-AGAG	5.759	28.390

	Mean	Stdev
rise-3-GCCA	0.627	0.471

	Mean	Stdev
roll-3-GCCA	-1.368	27.983

	Mean	Stdev
shift-3-GCCA	0.000	1.147

	Mean	Stdev
slide-3-GCCA	0.004	0.457

	Mean	Stdev
tilt-3-GCCA	27.704	59.664

	Mean	Stdev
twist-3-GCCA	7.558	34.791

	Mean	Stdev
rise-4-CCAC	1.127	0.473

	Mean	Stdev
roll-4-CCAC	7.138	45.538

	Mean	Stdev
shift-4-CCAC	0.000	1.231

	Mean	Stdev
slide-4-CCAC	-0.830	0.471

	Mean	Stdev
tilt-4-CCAC	30.959	49.920

	Mean	Stdev
twist-4-CCAC	8.033	39.980

	Mean	Stdev
rise-5-CCGA	2.677	2.117

	Mean	Stdev
roll-5-CCGA	-18.986	67.180

	Mean	Stdev
shift-5-CCGA	0.000	4.323

	Mean	Stdev
slide-5-CCGA	-4.352	1.024

	Mean	Stdev
tilt-5-CCGA	0.000	31.553

	Mean	Stdev
twist-5-CCGA	-37.205	49.139

	Mean	Stdev
rise-6-CUAG	3.450	0.315

	Mean	Stdev
roll-6-CUAG	11.951	5.746

	Mean	Stdev
shift-6-CUAG	0.000	1.529

	Mean	Stdev
slide-6-CUAG	-1.667	0.322

	Mean	Stdev
tilt-6-CUAG	0.000	6.038

	Mean	Stdev
twist-6-CUAG	33.850	2.938

	Mean	Stdev
rise-7-UGCA	3.336	0.310

	Mean	Stdev
roll-7-UGCA	21.067	5.772

	Mean	Stdev
shift-7-UGCA	0.000	0.593

	Mean	Stdev
slide-7-UGCA	-1.019	0.305

	Mean	Stdev
tilt-7-UGCA	0.000	4.553

	Mean	Stdev
twist-7-UGCA	35.716	2.758

	Mean	Stdev
rise-8-GGCC	3.341	0.267

	Mean	Stdev
roll-8-GGCC	18.198	4.883

	Mean	Stdev
shift-8-GGCC	0.000	0.670

	Mean	Stdev
slide-8-GGCC	-1.503	0.339

	Mean	Stdev
tilt-8-GGCC	0.000	3.969

	Mean	Stdev
twist-8-GGCC	30.922	3.017

	Mean	Stdev
rise-9-GGCC	3.417	0.284

	Mean	Stdev
roll-9-GGCC	17.337	5.160

	Mean	Stdev
shift-9-GGCC	0.000	0.589

	Mean	Stdev
slide-9-GGCC	-1.553	0.305

	Mean	Stdev
tilt-9-GGCC	0.000	4.050

	Mean	Stdev
twist-9-GGCC	31.189	2.927

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3'	C	-	G	-	A	-	C	-	A	-	G	-	A	-	C	-	C	-	C	-	U	-	G	-	G	-	U	-	C	-	G	5'
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