NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.768	0.000	0.000	-0.023	0.000	0.000
Slide	0.000	3.432	1.394	0.000	-0.096	-0.247
Rise	0.000	1.394	7.164	0.000	-0.069	-0.147
Tilt	-0.023	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.096	-0.069	0.000	0.027	0.010
Twist	0.000	-0.247	-0.147	0.000	0.010	0.048

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.885	0.000	0.000	0.017	0.000	0.000
Slide	0.000	4.934	2.607	0.000	-0.048	-0.236
Rise	0.000	2.607	9.605	0.000	0.018	-0.152
Tilt	0.017	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.048	0.018	0.000	0.034	0.008
Twist	0.000	-0.236	-0.152	0.000	0.008	0.069

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.173	0.000	0.000	-0.119	0.000	0.000
Slide	0.000	1.812	0.514	0.000	0.003	-0.053
Rise	0.000	0.514	6.623	0.000	-0.074	-0.206
Tilt	-0.119	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.003	-0.074	0.000	0.016	0.007
Twist	0.000	-0.053	-0.206	0.000	0.007	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.162	0.000	0.000	-0.107	0.000	0.000
Slide	0.000	1.802	0.585	0.000	0.004	-0.057
Rise	0.000	0.584	6.491	0.000	-0.067	-0.205
Tilt	-0.107	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.004	-0.067	0.000	0.017	0.007
Twist	0.000	-0.057	-0.205	0.000	0.007	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.948	0.000	0.000	-0.068	0.000	0.000
Slide	0.000	1.612	1.238	0.000	-0.040	-0.111
Rise	0.000	1.238	6.763	0.000	-0.044	-0.175
Tilt	-0.068	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.040	-0.044	0.000	0.023	0.012
Twist	0.000	-0.111	-0.175	0.000	0.012	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.252	0.000	0.000	-0.104	0.000	0.000
Slide	0.000	3.606	2.107	0.000	0.071	-0.144
Rise	0.000	2.107	9.672	0.000	0.127	-0.214
Tilt	-0.104	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.071	0.127	0.000	0.021	0.006
Twist	0.000	-0.144	-0.214	0.000	0.006	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.380	0.000	0.000	-0.114	0.000	0.000
Slide	0.000	3.833	2.122	0.000	0.074	-0.173
Rise	0.000	2.122	9.155	0.000	0.113	-0.228
Tilt	-0.114	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.074	0.113	0.000	0.021	0.008
Twist	0.000	-0.173	-0.228	0.000	0.008	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.907	0.000	0.000	-0.064	0.000	0.000
Slide	0.000	1.569	1.083	0.000	-0.039	-0.106
Rise	0.000	1.083	6.434	0.000	-0.052	-0.156
Tilt	-0.064	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.039	-0.052	0.000	0.022	0.011
Twist	0.000	-0.106	-0.156	0.000	0.011	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.772	0.000	0.000	-0.020	0.000	0.000
Slide	0.000	3.504	1.299	0.000	-0.096	-0.248
Rise	0.000	1.299	6.604	0.000	-0.063	-0.138
Tilt	-0.020	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.096	-0.063	0.000	0.026	0.009
Twist	0.000	-0.248	-0.138	0.000	0.009	0.047

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-atat.6.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-gatc.4.cte	0.000	0.000

	Mean	Stdev
-gcgc.10.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-tcga.8.cte	0.000	0.000

	Mean	Stdev
-ttaa.7.cte	0.000	0.000

	Mean	Stdev
rise-10-GCGC	3.368	0.299

	Mean	Stdev
roll-10-GCGC	-1.330	6.625

	Mean	Stdev
shift-10-GCGC	0.000	0.778

	Mean	Stdev
slide-10-GCGC	-0.117	0.498

	Mean	Stdev
tilt-10-GCGC	0.000	4.464

	Mean	Stdev
twist-10-GCGC	30.999	6.057

	Mean	Stdev
rise-2-GCGC	3.359	0.305

	Mean	Stdev
roll-2-GCGC	-0.945	6.662

	Mean	Stdev
shift-2-GCGC	0.000	0.753

	Mean	Stdev
slide-2-GCGC	-0.077	0.501

	Mean	Stdev
tilt-2-GCGC	0.000	4.573

	Mean	Stdev
twist-2-GCGC	30.870	5.814

	Mean	Stdev
rise-3-CGCG	3.178	0.383

	Mean	Stdev
roll-3-CGCG	7.937	6.892

	Mean	Stdev
shift-3-CGCG	0.000	0.959

	Mean	Stdev
slide-3-CGCG	0.025	0.609

	Mean	Stdev
tilt-3-CGCG	0.000	6.358

	Mean	Stdev
twist-3-CGCG	31.761	8.778

	Mean	Stdev
rise-4-GATC	3.419	0.332

	Mean	Stdev
roll-4-GATC	2.645	5.846

	Mean	Stdev
shift-4-GATC	0.000	0.856

	Mean	Stdev
slide-4-GATC	-0.151	0.732

	Mean	Stdev
tilt-4-GATC	0.000	4.752

	Mean	Stdev
twist-4-GATC	34.604	6.586

	Mean	Stdev
rise-5-AATT	3.364	0.300

	Mean	Stdev
roll-5-AATT	1.857	4.969

	Mean	Stdev
shift-5-AATT	0.000	0.579

	Mean	Stdev
slide-5-AATT	-0.587	0.539

	Mean	Stdev
tilt-5-AATT	0.000	4.745

	Mean	Stdev
twist-5-AATT	34.593	4.483

	Mean	Stdev
rise-6-ATAT	3.357	0.268

	Mean	Stdev
roll-6-ATAT	-1.037	4.222

	Mean	Stdev
shift-6-ATAT	0.000	0.559

	Mean	Stdev
slide-6-ATAT	-1.005	0.401

	Mean	Stdev
tilt-6-ATAT	0.000	3.616

	Mean	Stdev
twist-6-ATAT	31.911	3.212

	Mean	Stdev
rise-7-TTAA	3.360	0.312

	Mean	Stdev
roll-7-TTAA	1.879	5.084

	Mean	Stdev
shift-7-TTAA	0.000	0.578

	Mean	Stdev
slide-7-TTAA	-0.586	0.533

	Mean	Stdev
tilt-7-TTAA	0.000	4.772

	Mean	Stdev
twist-7-TTAA	34.491	4.488

	Mean	Stdev
rise-8-TCGA	3.423	0.333

	Mean	Stdev
roll-8-TCGA	2.686	5.903

	Mean	Stdev
shift-8-TCGA	0.000	0.872

	Mean	Stdev
slide-8-TCGA	-0.116	0.737

	Mean	Stdev
tilt-8-TCGA	0.000	4.765

	Mean	Stdev
twist-8-TCGA	34.951	6.668

	Mean	Stdev
rise-9-CGCG	3.155	0.387

	Mean	Stdev
roll-9-CGCG	8.169	6.711

	Mean	Stdev
shift-9-CGCG	0.000	0.920

	Mean	Stdev
slide-9-CGCG	0.017	0.617

	Mean	Stdev
tilt-9-CGCG	0.000	6.332

	Mean	Stdev
twist-9-CGCG	30.900	8.805

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