NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.367	0.000	0.000	-0.018	0.000	0.000
Slide	0.000	3.036	2.051	0.000	-0.099	-0.077
Rise	0.000	2.051	8.173	0.000	-0.025	-0.207
Tilt	-0.018	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.099	-0.025	0.000	0.029	0.008
Twist	0.000	-0.077	-0.207	0.000	0.008	0.039

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.938	0.000	0.000	0.044	0.000	0.000
Slide	0.000	1.922	1.348	0.000	-0.068	-0.060
Rise	0.000	1.348	7.094	0.000	0.007	-0.115
Tilt	0.044	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.068	0.007	0.000	0.026	0.010
Twist	0.000	-0.060	-0.115	0.000	0.010	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.143	0.000	0.000	-0.086	0.000	0.000
Slide	0.000	2.598	1.566	0.000	-0.011	-0.129
Rise	0.000	1.566	7.828	0.000	0.038	-0.200
Tilt	-0.086	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.011	0.038	0.000	0.022	0.009
Twist	0.000	-0.129	-0.200	0.000	0.009	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.613	0.000	0.000	-0.111	0.000	0.000
Slide	0.000	1.683	2.020	0.000	-0.055	-0.056
Rise	0.000	2.020	7.293	0.000	-0.064	-0.157
Tilt	-0.111	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.055	-0.064	0.000	0.028	0.015
Twist	0.000	-0.056	-0.157	0.000	0.015	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.693	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	2.342	2.301	0.000	-0.047	-0.212
Rise	0.000	2.301	6.364	0.000	0.021	-0.155
Tilt	-0.048	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.047	0.021	0.000	0.025	0.007
Twist	0.000	-0.212	-0.155	0.000	0.007	0.050

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.990	0.000	0.000	-0.083	0.000	0.000
Slide	0.000	3.430	2.366	0.000	-0.037	-0.162
Rise	0.000	2.366	6.758	0.000	-0.032	-0.137
Tilt	-0.083	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.037	-0.032	0.000	0.028	0.007
Twist	0.000	-0.162	-0.137	0.000	0.007	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.466	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	4.277	2.173	0.000	-0.020	-0.189
Rise	0.000	2.173	7.922	0.000	0.007	-0.180
Tilt	-0.029	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.020	0.007	0.000	0.030	0.009
Twist	0.000	-0.189	-0.180	0.000	0.009	0.061

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.021	0.000	0.000	-0.032	0.000	0.000
Slide	0.000	3.414	2.303	0.000	0.017	-0.152
Rise	0.000	2.303	8.478	0.000	0.053	-0.170
Tilt	-0.032	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.017	0.053	0.000	0.017	0.005
Twist	0.000	-0.152	-0.170	0.000	0.005	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.279	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	5.866	3.485	0.000	0.048	-0.300
Rise	0.000	3.485	10.389	0.000	0.157	-0.176
Tilt	-0.059	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.048	0.157	0.000	0.029	0.001
Twist	0.000	-0.300	-0.176	0.000	0.001	0.058

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.667	0.000	0.000	-0.082	0.000	0.000
Slide	0.000	2.340	1.385	0.000	-0.028	-0.198
Rise	0.000	1.385	5.301	0.000	0.025	-0.200
Tilt	-0.082	0.000	0.000	0.018	0.000	0.000
Roll	0.000	-0.028	0.025	0.000	0.016	0.010
Twist	0.000	-0.198	-0.200	0.000	0.010	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.521	0.000	0.000	-0.071	0.000	0.000
Slide	0.000	1.721	0.887	0.000	-0.003	-0.126
Rise	0.000	0.887	6.461	0.000	-0.068	-0.230
Tilt	-0.071	0.000	0.000	0.020	0.000	0.000
Roll	0.000	-0.003	-0.068	0.000	0.019	0.011
Twist	0.000	-0.126	-0.230	0.000	0.011	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.814	0.000	0.000	-0.003	0.000	0.000
Slide	0.000	2.466	1.799	0.000	-0.021	-0.201
Rise	0.000	1.799	5.361	0.000	-0.006	-0.194
Tilt	-0.003	0.000	0.000	0.011	0.000	0.000
Roll	0.000	-0.021	-0.006	0.000	0.014	0.007
Twist	0.000	-0.201	-0.194	0.000	0.007	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.590	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	3.371	0.483	0.000	-0.077	-0.277
Rise	0.000	0.483	7.062	0.000	-0.032	-0.184
Tilt	-0.081	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.077	-0.032	0.000	0.022	0.005
Twist	0.000	-0.277	-0.184	0.000	0.005	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.484	0.000	0.000	0.034	0.000	0.000
Slide	0.000	3.018	0.153	0.000	-0.108	-0.169
Rise	0.000	0.153	7.725	0.000	-0.015	-0.115
Tilt	0.034	0.000	0.000	0.019	0.000	0.000
Roll	0.000	-0.108	-0.015	0.000	0.019	0.014
Twist	0.000	-0.169	-0.115	0.000	0.014	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.927	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	2.500	-0.256	0.000	-0.104	-0.205
Rise	0.000	-0.256	6.951	0.000	0.028	-0.114
Tilt	-0.081	0.000	0.000	0.024	0.000	0.000
Roll	0.000	-0.104	0.028	0.000	0.023	0.010
Twist	0.000	-0.205	-0.114	0.000	0.010	0.035

[Image courtesy of Curves+]

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	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-agct.5.cte	0.000	0.000

	Mean	Stdev
-ggcc.12.cte	0.000	0.000

	Mean	Stdev
-ggcc.13.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
-ggcc.7.cte	0.000	0.000

	Mean	Stdev
-gtac.14.cte	0.000	0.000

	Mean	Stdev
-gtac.2.cte	0.000	0.000

	Mean	Stdev
-gtac.8.cte	0.000	0.000

	Mean	Stdev
-tata.10.cte	0.000	0.000

	Mean	Stdev
-tata.16.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-ttaa.15.cte	0.000	0.000

	Mean	Stdev
-ttaa.3.cte	0.000	0.000

	Mean	Stdev
-ttaa.9.cte	0.000	0.000

	Mean	Stdev
rise-10-TATA	3.382	0.385

	Mean	Stdev
roll-10-TATA	-4.396	6.752

	Mean	Stdev
shift-10-TATA	0.000	1.438

	Mean	Stdev
slide-10-TATA	0.502	0.642

	Mean	Stdev
tilt-10-TATA	0.000	8.826

	Mean	Stdev
twist-10-TATA	41.037	5.014

	Mean	Stdev
rise-11-AGCT	3.242	0.314

	Mean	Stdev
roll-11-AGCT	-0.846	4.959

	Mean	Stdev
shift-11-AGCT	0.000	0.657

	Mean	Stdev
slide-11-AGCT	-0.347	0.514

	Mean	Stdev
tilt-11-AGCT	0.000	4.060

	Mean	Stdev
twist-11-AGCT	31.593	4.310

	Mean	Stdev
rise-12-GGCC	3.276	0.312

	Mean	Stdev
roll-12-GGCC	2.112	5.634

	Mean	Stdev
shift-12-GGCC	0.000	0.790

	Mean	Stdev
slide-12-GGCC	0.188	0.539

	Mean	Stdev
tilt-12-GGCC	0.000	4.416

	Mean	Stdev
twist-12-GGCC	35.677	5.101

	Mean	Stdev
rise-13-GGCC	3.650	0.360

	Mean	Stdev
roll-13-GGCC	3.941	5.380

	Mean	Stdev
shift-13-GGCC	0.000	0.671

	Mean	Stdev
slide-13-GGCC	-0.429	0.740

	Mean	Stdev
tilt-13-GGCC	0.000	4.270

	Mean	Stdev
twist-13-GGCC	36.465	5.177

	Mean	Stdev
rise-14-GTAC	3.385	0.297

	Mean	Stdev
roll-14-GTAC	-1.852	4.565

	Mean	Stdev
shift-14-GTAC	0.000	0.637

	Mean	Stdev
slide-14-GTAC	-0.438	0.419

	Mean	Stdev
tilt-14-GTAC	0.000	3.931

	Mean	Stdev
twist-14-GTAC	33.769	3.453

	Mean	Stdev
rise-15-TTAA	3.359	0.310

	Mean	Stdev
roll-15-TTAA	-2.684	5.454

	Mean	Stdev
shift-15-TTAA	0.000	1.246

	Mean	Stdev
slide-15-TTAA	0.101	0.611

	Mean	Stdev
tilt-15-TTAA	0.000	5.430

	Mean	Stdev
twist-15-TTAA	35.155	5.260

	Mean	Stdev
rise-16-TATA	3.212	0.372

	Mean	Stdev
roll-16-TATA	0.205	7.093

	Mean	Stdev
shift-16-TATA	0.000	1.471

	Mean	Stdev
slide-16-TATA	0.867	0.804

	Mean	Stdev
tilt-16-TATA	0.000	7.434

	Mean	Stdev
twist-16-TATA	38.350	8.714

	Mean	Stdev
rise-2-GTAC	3.452	0.298

	Mean	Stdev
roll-2-GTAC	-0.016	6.103

	Mean	Stdev
shift-2-GTAC	0.000	0.769

	Mean	Stdev
slide-2-GTAC	-0.851	0.491

	Mean	Stdev
tilt-2-GTAC	0.000	4.280

	Mean	Stdev
twist-2-GTAC	30.825	4.628

	Mean	Stdev
rise-3-TTAA	3.126	0.284

	Mean	Stdev
roll-3-TTAA	2.472	6.737

	Mean	Stdev
shift-3-TTAA	0.000	0.642

	Mean	Stdev
slide-3-TTAA	-0.353	0.530

	Mean	Stdev
tilt-3-TTAA	0.000	5.618

	Mean	Stdev
twist-3-TTAA	30.592	5.260

	Mean	Stdev
rise-4-TATA	3.532	0.390

	Mean	Stdev
roll-4-TATA	-0.763	6.849

	Mean	Stdev
shift-4-TATA	0.000	0.849

	Mean	Stdev
slide-4-TATA	-0.305	0.724

	Mean	Stdev
tilt-4-TATA	0.000	7.364

	Mean	Stdev
twist-4-TATA	38.641	6.548

	Mean	Stdev
rise-5-AGCT	3.134	0.320

	Mean	Stdev
roll-5-AGCT	0.022	5.388

	Mean	Stdev
shift-5-AGCT	0.000	0.559

	Mean	Stdev
slide-5-AGCT	-0.724	0.630

	Mean	Stdev
tilt-5-AGCT	0.000	4.421

	Mean	Stdev
twist-5-AGCT	29.022	4.732

	Mean	Stdev
rise-6-GGCC	3.559	0.391

	Mean	Stdev
roll-6-GGCC	1.092	5.061

	Mean	Stdev
shift-6-GGCC	0.000	0.602

	Mean	Stdev
slide-6-GGCC	-0.932	0.788

	Mean	Stdev
tilt-6-GGCC	0.000	4.480

	Mean	Stdev
twist-6-GGCC	33.020	4.433

	Mean	Stdev
rise-7-GGCC	3.709	0.339

	Mean	Stdev
roll-7-GGCC	2.769	4.658

	Mean	Stdev
shift-7-GGCC	0.000	0.457

	Mean	Stdev
slide-7-GGCC	-1.204	0.504

	Mean	Stdev
tilt-7-GGCC	0.000	4.025

	Mean	Stdev
twist-7-GGCC	33.471	3.915

	Mean	Stdev
rise-8-GTAC	3.481	0.275

	Mean	Stdev
roll-8-GTAC	-5.759	4.694

	Mean	Stdev
shift-8-GTAC	0.000	0.517

	Mean	Stdev
slide-8-GTAC	-0.744	0.401

	Mean	Stdev
tilt-8-GTAC	0.000	3.819

	Mean	Stdev
twist-8-GTAC	35.169	3.710

	Mean	Stdev
rise-9-TTAA	3.226	0.316

	Mean	Stdev
roll-9-TTAA	-4.012	5.685

	Mean	Stdev
shift-9-TTAA	0.000	0.942

	Mean	Stdev
slide-9-TTAA	-0.000	0.724

	Mean	Stdev
tilt-9-TTAA	0.000	5.802

	Mean	Stdev
twist-9-TTAA	35.120	6.151

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