NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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5'	C	-	T	-	C	-	G	-	G	-	C	-	T	-	A	-	T	-	T	-	A	-	A	-	T	-	A	-	G	-	C	-	C	-	G	-	A	-	G	3'
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3'	G	-	A	-	G	-	C	-	C	-	G	-	A	-	T	-	A	-	A	-	T	-	T	-	A	-	T	-	C	-	G	-	G	-	C	-	T	-	C	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.855	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	2.702	1.197	0.000	-0.050	-0.164
Rise	0.000	1.197	9.273	0.000	-0.024	-0.160
Tilt	-0.094	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.050	-0.024	0.000	0.020	0.009
Twist	0.000	-0.164	-0.160	0.000	0.009	0.045

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.942	0.000	0.000	-0.123	0.000	0.000
Slide	0.000	2.657	1.671	0.000	-0.045	-0.136
Rise	0.000	1.671	9.099	0.000	-0.025	-0.203
Tilt	-0.123	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.045	-0.025	0.000	0.021	0.005
Twist	0.000	-0.136	-0.203	0.000	0.005	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.465	0.000	0.000	0.009	0.000	0.000
Slide	0.000	4.659	2.760	0.000	-0.029	-0.115
Rise	0.000	2.760	11.116	0.000	0.057	-0.144
Tilt	0.009	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.029	0.057	0.000	0.029	0.014
Twist	0.000	-0.115	-0.144	0.000	0.014	0.055

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.357	0.000	0.000	0.012	0.000	0.000
Slide	0.000	4.853	3.150	0.000	-0.027	-0.118
Rise	0.000	3.150	11.213	0.000	0.057	-0.116
Tilt	0.012	0.000	0.000	0.042	0.000	0.000
Roll	0.000	-0.027	0.057	0.000	0.028	0.014
Twist	0.000	-0.118	-0.116	0.000	0.014	0.053

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.112	0.000	0.000	-0.119	0.000	0.000
Slide	0.000	1.774	1.824	0.000	-0.014	-0.098
Rise	0.000	1.824	9.495	0.000	-0.022	-0.186
Tilt	-0.119	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.014	-0.022	0.000	0.022	0.007
Twist	0.000	-0.098	-0.186	0.000	0.007	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.069	0.000	0.000	-0.115	0.000	0.000
Slide	0.000	3.172	0.205	0.000	0.017	-0.139
Rise	0.000	0.205	9.180	0.000	-0.117	-0.277
Tilt	-0.115	0.000	0.000	0.031	0.000	0.000
Roll	0.000	0.017	-0.117	0.000	0.020	0.009
Twist	0.000	-0.139	-0.277	0.000	0.009	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.996	0.000	0.000	-0.105	0.000	0.000
Slide	0.000	3.317	0.370	0.000	-0.003	-0.168
Rise	0.000	0.370	9.598	0.000	-0.112	-0.289
Tilt	-0.105	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.003	-0.112	0.000	0.021	0.010
Twist	0.000	-0.168	-0.289	0.000	0.010	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.854	0.000	0.000	-0.115	0.000	0.000
Slide	0.000	2.457	1.686	0.000	-0.050	-0.125
Rise	0.000	1.686	9.140	0.000	-0.031	-0.213
Tilt	-0.115	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.050	-0.031	0.000	0.021	0.006
Twist	0.000	-0.125	-0.213	0.000	0.006	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.871	0.000	0.000	-0.108	0.000	0.000
Slide	0.000	2.654	0.965	0.000	-0.025	-0.154
Rise	0.000	0.965	8.747	0.000	-0.016	-0.173
Tilt	-0.108	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.025	-0.016	0.000	0.020	0.007
Twist	0.000	-0.154	-0.173	0.000	0.007	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.279	0.000	0.000	-0.124	0.000	0.000
Slide	0.000	3.399	3.416	0.000	0.035	-0.128
Rise	0.000	3.416	12.358	0.000	0.113	-0.259
Tilt	-0.124	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.035	0.113	0.000	0.031	0.007
Twist	0.000	-0.128	-0.259	0.000	0.007	0.044

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.172	0.000	0.000	-0.124	0.000	0.000
Slide	0.000	3.439	3.304	0.000	0.035	-0.112
Rise	0.000	3.304	11.703	0.000	0.113	-0.232
Tilt	-0.124	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.035	0.113	0.000	0.029	0.005
Twist	0.000	-0.112	-0.232	0.000	0.005	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.124	0.000	0.000	-0.107	0.000	0.000
Slide	0.000	1.841	1.778	0.000	-0.004	-0.117
Rise	0.000	1.778	9.011	0.000	-0.027	-0.184
Tilt	-0.107	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.004	-0.027	0.000	0.022	0.007
Twist	0.000	-0.117	-0.184	0.000	0.007	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.546	0.000	0.000	-0.045	0.000	0.000
Slide	0.000	2.337	1.025	0.000	0.016	-0.127
Rise	0.000	1.026	8.088	0.000	-0.137	-0.246
Tilt	-0.045	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.016	-0.137	0.000	0.019	0.014
Twist	0.000	-0.127	-0.246	0.000	0.014	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.423	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	2.619	1.054	0.000	0.023	-0.135
Rise	0.000	1.054	7.923	0.000	-0.079	-0.227
Tilt	-0.054	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.023	-0.079	0.000	0.017	0.011
Twist	0.000	-0.135	-0.227	0.000	0.011	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.390	0.000	0.000	-0.049	0.000	0.000
Slide	0.000	2.264	1.005	0.000	0.026	-0.108
Rise	0.000	1.005	8.101	0.000	-0.108	-0.252
Tilt	-0.049	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.026	-0.108	0.000	0.019	0.013
Twist	0.000	-0.108	-0.252	0.000	0.013	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.916	0.000	0.000	-0.122	0.000	0.000
Slide	0.000	2.687	1.090	0.000	-0.026	-0.098
Rise	0.000	1.090	9.186	0.000	-0.024	-0.180
Tilt	-0.122	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.026	-0.024	0.000	0.024	0.011
Twist	0.000	-0.098	-0.180	0.000	0.011	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.862	0.000	0.000	-0.079	0.000	0.000
Slide	0.000	2.680	1.178	0.000	-0.065	-0.179
Rise	0.000	1.178	9.011	0.000	-0.036	-0.153
Tilt	-0.079	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.065	-0.036	0.000	0.019	0.006
Twist	0.000	-0.179	-0.153	0.000	0.006	0.033

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.11.cte	0.000	0.000

	Mean	Stdev
-agct.14.cte	0.000	0.000

	Mean	Stdev
-atat.12.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-ccgg.16.cte	0.000	0.000

	Mean	Stdev
-cgcg.17.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-ctag.6.cte	0.000	0.000

	Mean	Stdev
-gatc.18.cte	0.000	0.000

	Mean	Stdev
-gcgc.15.cte	0.000	0.000

	Mean	Stdev
-gcgc.5.cte	0.000	0.000

	Mean	Stdev
-ggcc.4.cte	0.000	0.000

	Mean	Stdev
-tata.10.cte	0.000	0.000

	Mean	Stdev
-tata.13.cte	0.000	0.000

	Mean	Stdev
-tata.7.cte	0.000	0.000

	Mean	Stdev
-tcga.2.cte	0.000	0.000

	Mean	Stdev
-ttaa.9.cte	0.000	0.000

	Mean	Stdev
rise-10-TATA	3.254	0.313

	Mean	Stdev
roll-10-TATA	9.230	8.009

	Mean	Stdev
shift-10-TATA	0.000	1.117

	Mean	Stdev
slide-10-TATA	-0.136	0.663

	Mean	Stdev
tilt-10-TATA	0.000	5.020

	Mean	Stdev
twist-10-TATA	30.506	7.551

	Mean	Stdev
rise-11-AATT	3.282	0.262

	Mean	Stdev
roll-11-AATT	-0.186	5.635

	Mean	Stdev
shift-11-AATT	0.000	1.021

	Mean	Stdev
slide-11-AATT	-0.100	0.535

	Mean	Stdev
tilt-11-AATT	0.000	5.457

	Mean	Stdev
twist-11-AATT	37.592	4.237

	Mean	Stdev
rise-12-ATAT	3.280	0.255

	Mean	Stdev
roll-12-ATAT	-2.254	4.845

	Mean	Stdev
shift-12-ATAT	0.000	0.633

	Mean	Stdev
slide-12-ATAT	-0.835	0.391

	Mean	Stdev
tilt-12-ATAT	0.000	3.661

	Mean	Stdev
twist-12-ATAT	32.125	3.594

	Mean	Stdev
rise-13-TATA	3.207	0.308

	Mean	Stdev
roll-13-TATA	4.281	7.614

	Mean	Stdev
shift-13-TATA	0.000	1.369

	Mean	Stdev
slide-13-TATA	0.097	0.610

	Mean	Stdev
tilt-13-TATA	0.000	5.465

	Mean	Stdev
twist-13-TATA	34.940	6.980

	Mean	Stdev
rise-14-AGCT	3.276	0.281

	Mean	Stdev
roll-14-AGCT	2.434	5.421

	Mean	Stdev
shift-14-AGCT	0.000	1.040

	Mean	Stdev
slide-14-AGCT	-0.206	0.550

	Mean	Stdev
tilt-14-AGCT	0.000	5.179

	Mean	Stdev
twist-14-AGCT	34.390	5.337

	Mean	Stdev
rise-15-GCGC	3.544	0.269

	Mean	Stdev
roll-15-GCGC	-1.122	4.599

	Mean	Stdev
shift-15-GCGC	0.000	0.554

	Mean	Stdev
slide-15-GCGC	-0.750	0.498

	Mean	Stdev
tilt-15-GCGC	0.000	4.110

	Mean	Stdev
twist-15-GCGC	37.291	4.136

	Mean	Stdev
rise-16-CCGG	3.447	0.282

	Mean	Stdev
roll-16-CCGG	2.546	5.334

	Mean	Stdev
shift-16-CCGG	0.000	0.877

	Mean	Stdev
slide-16-CCGG	-0.248	0.674

	Mean	Stdev
tilt-16-CCGG	0.000	4.592

	Mean	Stdev
twist-16-CCGG	36.000	4.853

	Mean	Stdev
rise-17-CGCG	2.993	0.322

	Mean	Stdev
roll-17-CGCG	5.873	6.099

	Mean	Stdev
shift-17-CGCG	0.000	0.954

	Mean	Stdev
slide-17-CGCG	0.166	0.539

	Mean	Stdev
tilt-17-CGCG	0.000	5.569

	Mean	Stdev
twist-17-CGCG	30.653	7.392

	Mean	Stdev
rise-18-GATC	3.306	0.276

	Mean	Stdev
roll-18-GATC	-0.137	5.594

	Mean	Stdev
shift-18-GATC	0.000	1.051

	Mean	Stdev
slide-18-GATC	0.187	0.562

	Mean	Stdev
tilt-18-GATC	0.000	5.285

	Mean	Stdev
twist-18-GATC	35.837	5.689

	Mean	Stdev
rise-2-TCGA	3.323	0.269

	Mean	Stdev
roll-2-TCGA	0.953	5.372

	Mean	Stdev
shift-2-TCGA	0.000	1.063

	Mean	Stdev
slide-2-TCGA	0.312	0.496

	Mean	Stdev
tilt-2-TCGA	0.000	5.280

	Mean	Stdev
twist-2-TCGA	37.255	4.661

	Mean	Stdev
rise-3-CGCG	2.950	0.314

	Mean	Stdev
roll-3-CGCG	5.626	6.021

	Mean	Stdev
shift-3-CGCG	0.000	0.961

	Mean	Stdev
slide-3-CGCG	0.170	0.550

	Mean	Stdev
tilt-3-CGCG	0.000	5.471

	Mean	Stdev
twist-3-CGCG	29.372	7.450

	Mean	Stdev
rise-4-GGCC	3.481	0.287

	Mean	Stdev
roll-4-GGCC	2.200	5.374

	Mean	Stdev
shift-4-GGCC	0.000	0.837

	Mean	Stdev
slide-4-GGCC	-0.399	0.679

	Mean	Stdev
tilt-4-GGCC	0.000	4.429

	Mean	Stdev
twist-4-GGCC	36.044	4.765

	Mean	Stdev
rise-5-GCGC	3.521	0.275

	Mean	Stdev
roll-5-GCGC	-0.769	4.702

	Mean	Stdev
shift-5-GCGC	0.000	0.572

	Mean	Stdev
slide-5-GCGC	-0.757	0.490

	Mean	Stdev
tilt-5-GCGC	0.000	4.191

	Mean	Stdev
twist-5-GCGC	36.580	4.227

	Mean	Stdev
rise-6-CTAG	3.278	0.284

	Mean	Stdev
roll-6-CTAG	2.654	5.502

	Mean	Stdev
shift-6-CTAG	0.000	1.092

	Mean	Stdev
slide-6-CTAG	-0.239	0.569

	Mean	Stdev
tilt-6-CTAG	0.000	5.273

	Mean	Stdev
twist-6-CTAG	34.592	5.339

	Mean	Stdev
rise-7-TATA	3.203	0.314

	Mean	Stdev
roll-7-TATA	4.889	7.355

	Mean	Stdev
shift-7-TATA	0.000	1.404

	Mean	Stdev
slide-7-TATA	0.131	0.629

	Mean	Stdev
tilt-7-TATA	0.000	5.412

	Mean	Stdev
twist-7-TATA	35.342	6.969

	Mean	Stdev
rise-8-ATAT	3.277	0.258

	Mean	Stdev
roll-8-ATAT	-2.037	5.017

	Mean	Stdev
shift-8-ATAT	0.000	0.658

	Mean	Stdev
slide-8-ATAT	-0.836	0.392

	Mean	Stdev
tilt-8-ATAT	0.000	3.746

	Mean	Stdev
twist-8-ATAT	32.090	3.673

	Mean	Stdev
rise-9-TTAA	3.279	0.267

	Mean	Stdev
roll-9-TTAA	0.222	5.753

	Mean	Stdev
shift-9-TTAA	0.000	0.966

	Mean	Stdev
slide-9-TTAA	-0.229	0.599

	Mean	Stdev
tilt-9-TTAA	0.000	5.493

	Mean	Stdev
twist-9-TTAA	36.199	5.390

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5'	C	-	T	-	C	-	G	-	G	-	C	-	T	-	A	-	T	-	T	-	A	-	A	-	T	-	A	-	G	-	C	-	C	-	G	-	A	-	G	3'
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5'	C	-	T	-	C	-	G	-	G	-	C	-	T	-	A	-	T	-	T	-	A	-	A	-	T	-	A	-	G	-	C	-	C	-	G	-	A	-	G	3'
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	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20
5'	C	-	T	-	C	-	G	-	G	-	C	-	T	-	A	-	T	-	T	-	A	-	A	-	T	-	A	-	G	-	C	-	C	-	G	-	A	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	A	-	G	-	C	-	C	-	G	-	A	-	T	-	A	-	A	-	T	-	T	-	A	-	T	-	C	-	G	-	G	-	C	-	T	-	C	5'
	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21