NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16
5'	C	-	T	-	G	-	C	-	T	-	A	-	T	-	A	-	A	-	A	-	A	-	G	-	G	-	C	-	T	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	A	-	C	-	G	-	A	-	T	-	A	-	T	-	T	-	T	-	T	-	C	-	C	-	G	-	A	-	C	5'
	32		31		30		29		28		27		26		25		24		23		22		21		20		19		18		17

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.744	0.373	0.733	-0.044	0.028	0.054
Slide	0.373	2.535	1.112	-0.004	-0.071	-0.121
Rise	0.733	1.112	9.831	-0.263	-0.034	-0.185
Tilt	-0.044	-0.004	-0.263	0.047	-0.001	0.008
Roll	0.028	-0.071	-0.034	-0.001	0.022	0.009
Twist	0.054	-0.121	-0.185	0.008	0.009	0.033

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.514	0.677	0.536	-0.033	0.008	0.043
Slide	0.677	2.682	1.165	-0.011	-0.083	-0.132
Rise	0.536	1.164	9.166	-0.255	-0.049	-0.203
Tilt	-0.033	-0.011	-0.255	0.046	0.001	0.010
Roll	0.008	-0.083	-0.049	0.001	0.022	0.010
Twist	0.043	-0.132	-0.203	0.010	0.010	0.034

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.561	0.399	0.509	-0.035	0.017	0.033
Slide	0.399	2.492	1.641	-0.016	-0.053	-0.124
Rise	0.509	1.641	8.933	-0.237	-0.056	-0.218
Tilt	-0.035	-0.016	-0.237	0.043	0.001	0.008
Roll	0.017	-0.053	-0.056	0.001	0.021	0.007
Twist	0.033	-0.124	-0.218	0.008	0.007	0.029

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.481	-0.106	0.509	-0.076	0.051	0.085
Slide	-0.106	1.929	1.553	0.006	-0.057	-0.104
Rise	0.509	1.553	8.224	-0.202	-0.023	-0.174
Tilt	-0.076	0.006	-0.202	0.045	0.003	0.007
Roll	0.051	-0.057	-0.023	0.003	0.023	0.009
Twist	0.085	-0.104	-0.174	0.007	0.009	0.035

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.086	-0.140	0.007	0.018	0.021	0.012
Slide	-0.140	4.325	2.586	-0.007	-0.035	-0.061
Rise	0.007	2.586	11.653	-0.000	0.106	-0.114
Tilt	0.018	-0.007	-0.000	0.041	0.001	-0.001
Roll	0.021	-0.035	0.106	0.001	0.025	0.010
Twist	0.012	-0.061	-0.114	-0.001	0.010	0.033

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.507	-0.646	-0.438	-0.053	-0.034	-0.072
Slide	-0.646	2.686	1.629	0.024	-0.030	-0.107
Rise	-0.438	1.629	8.768	0.230	-0.048	-0.240
Tilt	-0.053	0.024	0.230	0.048	-0.004	-0.012
Roll	-0.034	-0.030	-0.048	-0.004	0.020	0.007
Twist	-0.072	-0.107	-0.240	-0.012	0.007	0.033

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.494	-0.624	-0.370	-0.051	-0.025	-0.081
Slide	-0.624	2.396	1.581	0.004	-0.031	-0.077
Rise	-0.370	1.581	9.190	0.180	-0.044	-0.224
Tilt	-0.051	0.004	0.180	0.045	-0.003	-0.010
Roll	-0.025	-0.031	-0.044	-0.003	0.020	0.007
Twist	-0.081	-0.077	-0.224	-0.010	0.007	0.038

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.229	-0.072	-0.108	-0.108	-0.007	-0.025
Slide	-0.072	3.104	2.865	0.050	0.029	-0.097
Rise	-0.108	2.865	11.730	0.050	0.146	-0.217
Tilt	-0.108	0.050	0.050	0.041	0.002	-0.002
Roll	-0.007	0.029	0.146	0.002	0.030	0.006
Twist	-0.025	-0.097	-0.217	-0.002	0.006	0.039

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.595	0.486	0.033	-0.126	0.001	-0.024
Slide	0.486	2.802	2.384	0.027	0.049	-0.062
Rise	0.033	2.384	11.105	-0.020	0.112	-0.194
Tilt	-0.126	0.027	-0.020	0.045	0.000	-0.002
Roll	0.001	0.049	0.112	0.000	0.025	0.006
Twist	-0.024	-0.062	-0.194	-0.002	0.006	0.034

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.743	0.274	0.756	-0.136	0.029	0.038
Slide	0.274	1.705	1.505	0.022	0.001	-0.101
Rise	0.756	1.505	8.919	-0.261	0.012	-0.151
Tilt	-0.136	0.022	-0.261	0.049	-0.003	-0.001
Roll	0.029	0.001	0.012	-0.003	0.022	0.004
Twist	0.038	-0.101	-0.151	-0.001	0.004	0.032

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.473	0.097	-0.260	-0.054	0.003	-0.009
Slide	0.097	2.272	0.934	0.006	0.007	-0.141
Rise	-0.260	0.934	7.521	0.015	-0.085	-0.212
Tilt	-0.054	0.006	0.015	0.025	-0.000	-0.000
Roll	0.003	0.007	-0.085	-0.000	0.015	0.011
Twist	-0.009	-0.141	-0.212	-0.000	0.011	0.032

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.474	-0.127	0.173	-0.050	0.002	0.000
Slide	-0.127	1.831	1.052	-0.003	0.001	-0.112
Rise	0.173	1.052	6.712	-0.016	-0.069	-0.182
Tilt	-0.050	-0.003	-0.016	0.024	-0.001	-0.000
Roll	0.002	0.001	-0.069	-0.001	0.013	0.008
Twist	0.000	-0.112	-0.182	-0.000	0.008	0.025

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.779	-0.121	-0.315	-0.087	-0.003	0.027
Slide	-0.121	2.607	0.866	0.025	0.019	-0.120
Rise	-0.315	0.866	7.414	-0.005	-0.078	-0.221
Tilt	-0.087	0.025	-0.005	0.029	0.003	-0.002
Roll	-0.003	0.019	-0.078	0.003	0.016	0.008
Twist	0.027	-0.120	-0.221	-0.002	0.008	0.027

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-aatt.9.cte	0.000	0.000

	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-atat.6.cte	0.000	0.000

	Mean	Stdev
-ctag.14.cte	0.000	0.000

	Mean	Stdev
-ctag.4.cte	0.000	0.000

	Mean	Stdev
-gcgc.13.cte	0.000	0.000

	Mean	Stdev
-gcgc.3.cte	0.000	0.000

	Mean	Stdev
-ggcc.12.cte	0.000	0.000

	Mean	Stdev
-tata.5.cte	0.000	0.000

	Mean	Stdev
-tata.7.cte	0.000	0.000

	Mean	Stdev
-tgca.2.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.259	0.283

	Mean	Stdev
roll-10-AATT	0.646	5.606

	Mean	Stdev
shift-10-AATT	-0.085	0.659

	Mean	Stdev
slide-10-AATT	-0.486	0.576

	Mean	Stdev
tilt-10-AATT	-2.743	3.937

	Mean	Stdev
twist-10-AATT	34.324	5.376

	Mean	Stdev
rise-11-AGCT	3.335	0.321

	Mean	Stdev
roll-11-AGCT	1.996	5.602

	Mean	Stdev
shift-11-AGCT	-0.021	0.776

	Mean	Stdev
slide-11-AGCT	-0.788	0.664

	Mean	Stdev
tilt-11-AGCT	-1.597	4.203

	Mean	Stdev
twist-11-AGCT	32.922	5.261

	Mean	Stdev
rise-12-GGCC	3.561	0.318

	Mean	Stdev
roll-12-GGCC	3.116	5.300

	Mean	Stdev
shift-12-GGCC	-0.172	0.708

	Mean	Stdev
slide-12-GGCC	-0.956	0.746

	Mean	Stdev
tilt-12-GGCC	0.953	4.486

	Mean	Stdev
twist-12-GGCC	34.514	5.102

	Mean	Stdev
rise-13-GCGC	3.499	0.272

	Mean	Stdev
roll-13-GCGC	-0.670	4.713

	Mean	Stdev
shift-13-GCGC	-0.093	0.552

	Mean	Stdev
slide-13-GCGC	-0.827	0.503

	Mean	Stdev
tilt-13-GCGC	-1.568	4.087

	Mean	Stdev
twist-13-GCGC	36.223	4.314

	Mean	Stdev
rise-14-CTAG	3.297	0.310

	Mean	Stdev
roll-14-CTAG	1.745	5.833

	Mean	Stdev
shift-14-CTAG	0.588	0.852

	Mean	Stdev
slide-14-CTAG	-0.290	0.604

	Mean	Stdev
tilt-14-CTAG	3.760	4.103

	Mean	Stdev
twist-14-CTAG	34.431	6.139

	Mean	Stdev
rise-2-TGCA	3.222	0.326

	Mean	Stdev
roll-2-TGCA	6.015	6.954

	Mean	Stdev
shift-2-TGCA	0.275	1.092

	Mean	Stdev
slide-2-TGCA	-0.022	0.565

	Mean	Stdev
tilt-2-TGCA	0.412	5.603

	Mean	Stdev
twist-2-TGCA	33.392	6.682

	Mean	Stdev
rise-3-GCGC	3.443	0.277

	Mean	Stdev
roll-3-GCGC	-1.013	5.238

	Mean	Stdev
shift-3-GCGC	-0.365	0.739

	Mean	Stdev
slide-3-GCGC	-0.472	0.544

	Mean	Stdev
tilt-3-GCGC	-2.166	4.283

	Mean	Stdev
twist-3-GCGC	35.045	4.703

	Mean	Stdev
rise-4-CTAG	3.299	0.293

	Mean	Stdev
roll-4-CTAG	2.569	5.689

	Mean	Stdev
shift-4-CTAG	0.698	0.818

	Mean	Stdev
slide-4-CTAG	-0.328	0.611

	Mean	Stdev
tilt-4-CTAG	4.183	4.107

	Mean	Stdev
twist-4-CTAG	35.112	5.252

	Mean	Stdev
rise-5-TATA	3.225	0.316

	Mean	Stdev
roll-5-TATA	4.807	7.923

	Mean	Stdev
shift-5-TATA	-0.251	1.321

	Mean	Stdev
slide-5-TATA	0.127	0.649

	Mean	Stdev
tilt-5-TATA	-0.367	5.559

	Mean	Stdev
twist-5-TATA	35.368	6.509

	Mean	Stdev
rise-6-ATAT	3.160	0.256

	Mean	Stdev
roll-6-ATAT	-1.543	5.445

	Mean	Stdev
shift-6-ATAT	0.207	0.744

	Mean	Stdev
slide-6-ATAT	-0.711	0.404

	Mean	Stdev
tilt-6-ATAT	-0.036	3.818

	Mean	Stdev
twist-6-ATAT	29.971	4.654

	Mean	Stdev
rise-7-TATA	3.307	0.335

	Mean	Stdev
roll-7-TATA	5.505	7.626

	Mean	Stdev
shift-7-TATA	0.172	1.300

	Mean	Stdev
slide-7-TATA	0.103	0.715

	Mean	Stdev
tilt-7-TATA	2.669	5.682

	Mean	Stdev
twist-7-TATA	35.691	6.783

	Mean	Stdev
rise-8-AATT	3.275	0.294

	Mean	Stdev
roll-8-AATT	2.491	5.668

	Mean	Stdev
shift-8-AATT	-0.434	0.753

	Mean	Stdev
slide-8-AATT	-0.165	0.617

	Mean	Stdev
tilt-8-AATT	-2.657	4.036

	Mean	Stdev
twist-8-AATT	34.080	5.603

	Mean	Stdev
rise-9-AATT	3.367	0.309

	Mean	Stdev
roll-9-AATT	1.414	5.499

	Mean	Stdev
shift-9-AATT	-0.258	0.676

	Mean	Stdev
slide-9-AATT	-0.446	0.595

	Mean	Stdev
tilt-9-AATT	-1.476	4.127

	Mean	Stdev
twist-9-AATT	34.798	5.747

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16
5'	C	-	T	-	G	-	C	-	T	-	A	-	T	-	A	-	A	-	A	-	A	-	G	-	G	-	C	-	T	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	A	-	C	-	G	-	A	-	T	-	A	-	T	-	T	-	T	-	T	-	C	-	C	-	G	-	A	-	C	5'
	32		31		30		29		28		27		26		25		24		23		22		21		20		19		18		17

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16
5'	C	-	T	-	G	-	C	-	T	-	A	-	T	-	A	-	A	-	A	-	A	-	G	-	G	-	C	-	T	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	A	-	C	-	G	-	A	-	T	-	A	-	T	-	T	-	T	-	T	-	C	-	C	-	G	-	A	-	C	5'
	32		31		30		29		28		27		26		25		24		23		22		21		20		19		18		17

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16
5'	C	-	T	-	G	-	C	-	T	-	A	-	T	-	A	-	A	-	A	-	A	-	G	-	G	-	C	-	T	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	A	-	C	-	G	-	A	-	T	-	A	-	T	-	T	-	T	-	T	-	C	-	C	-	G	-	A	-	C	5'
	32		31		30		29		28		27		26		25		24		23		22		21		20		19		18		17