NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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3'	T	-	T	-	A	-	G	-	G	-	G	-	C	-	A	-	A	-	A	-	G	-	G	-	T	-	T	-	G	-	C	-	T	-	T	-	C	-	C	-	G	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.860	0.000	0.000	-0.103	0.000	0.000
Slide	0.000	2.706	0.750	0.000	-0.063	-0.162
Rise	0.000	0.750	9.661	0.000	-0.020	-0.156
Tilt	-0.103	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.063	-0.020	0.000	0.022	0.012
Twist	0.000	-0.162	-0.156	0.000	0.012	0.039

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.255	0.000	0.000	-0.010	0.000	0.000
Slide	0.000	2.673	0.747	0.000	-0.081	-0.111
Rise	0.000	0.747	9.363	0.000	-0.059	-0.164
Tilt	-0.010	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.081	-0.059	0.000	0.022	0.008
Twist	0.000	-0.111	-0.164	0.000	0.008	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.089	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	3.962	3.353	0.000	0.012	-0.082
Rise	0.000	3.353	11.058	0.000	0.061	-0.241
Tilt	-0.053	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.012	0.061	0.000	0.024	0.008
Twist	0.000	-0.082	-0.241	0.000	0.008	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.183	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	2.538	1.636	0.000	-0.049	-0.137
Rise	0.000	1.636	7.641	0.000	-0.021	-0.180
Tilt	-0.095	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.049	-0.021	0.000	0.022	0.004
Twist	0.000	-0.137	-0.180	0.000	0.004	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.799	0.000	0.000	-0.015	0.000	0.000
Slide	0.000	5.008	2.581	0.000	-0.040	-0.112
Rise	0.000	2.581	9.885	0.000	0.026	-0.192
Tilt	-0.015	0.000	0.000	0.047	0.000	0.000
Roll	0.000	-0.040	0.026	0.000	0.032	0.010
Twist	0.000	-0.112	-0.192	0.000	0.010	0.048

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.739	0.000	0.000	-0.060	0.000	0.000
Slide	0.000	2.865	0.845	0.000	-0.004	-0.139
Rise	0.000	0.844	8.131	0.000	-0.106	-0.213
Tilt	-0.060	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.004	-0.106	0.000	0.019	0.010
Twist	0.000	-0.139	-0.213	0.000	0.010	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.884	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	1.683	1.754	0.000	-0.009	-0.085
Rise	0.000	1.754	8.572	0.000	-0.025	-0.174
Tilt	-0.094	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.009	-0.025	0.000	0.018	0.007
Twist	0.000	-0.085	-0.174	0.000	0.007	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.037	0.000	0.000	-0.119	0.000	0.000
Slide	0.000	1.631	1.783	0.000	0.016	-0.077
Rise	0.000	1.783	8.215	0.000	-0.057	-0.194
Tilt	-0.119	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.016	-0.057	0.000	0.019	0.006
Twist	0.000	-0.077	-0.194	0.000	0.006	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.901	0.000	0.000	-0.131	0.000	0.000
Slide	0.000	2.143	1.674	0.000	-0.028	-0.112
Rise	0.000	1.674	9.524	0.000	-0.017	-0.207
Tilt	-0.131	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.028	-0.017	0.000	0.023	0.008
Twist	0.000	-0.112	-0.207	0.000	0.008	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.027	0.000	0.000	-0.131	0.000	0.000
Slide	0.000	2.429	0.880	0.000	0.012	-0.081
Rise	0.000	0.880	7.104	0.000	-0.075	-0.221
Tilt	-0.131	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.012	-0.075	0.000	0.015	0.005
Twist	0.000	-0.081	-0.221	0.000	0.005	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.946	0.000	0.000	-0.112	0.000	0.000
Slide	0.000	2.943	0.850	0.000	-0.002	-0.139
Rise	0.000	0.850	8.469	0.000	-0.085	-0.241
Tilt	-0.112	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.002	-0.085	0.000	0.019	0.006
Twist	0.000	-0.139	-0.241	0.000	0.006	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.844	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	1.914	1.456	0.000	-0.055	-0.105
Rise	0.000	1.456	7.893	0.000	-0.065	-0.176
Tilt	-0.094	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.055	-0.065	0.000	0.025	0.012
Twist	0.000	-0.105	-0.176	0.000	0.012	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.716	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	1.971	1.643	0.000	0.007	-0.092
Rise	0.000	1.643	7.183	0.000	-0.032	-0.122
Tilt	-0.090	0.000	0.000	0.034	0.000	0.000
Roll	0.000	0.007	-0.032	0.000	0.022	0.004
Twist	0.000	-0.092	-0.122	0.000	0.004	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.158	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	3.613	2.791	0.000	0.038	-0.084
Rise	0.000	2.791	11.052	0.000	0.080	-0.200
Tilt	-0.026	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.038	0.080	0.000	0.025	0.006
Twist	0.000	-0.084	-0.200	0.000	0.006	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.099	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.149	1.750	0.000	-0.039	-0.110
Rise	0.000	1.750	8.249	0.000	-0.070	-0.189
Tilt	-0.088	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.039	-0.070	0.000	0.021	0.010
Twist	0.000	-0.110	-0.189	0.000	0.010	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.030	0.000	0.000	-0.105	0.000	0.000
Slide	0.000	2.040	1.365	0.000	-0.021	-0.119
Rise	0.000	1.365	8.309	0.000	-0.058	-0.153
Tilt	-0.105	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.021	-0.058	0.000	0.024	0.008
Twist	0.000	-0.119	-0.153	0.000	0.008	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.622	0.000	0.000	-0.096	0.000	0.000
Slide	0.000	3.345	1.033	0.000	-0.052	-0.229
Rise	0.000	1.033	8.532	0.000	-0.051	-0.152
Tilt	-0.096	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.052	-0.051	0.000	0.023	0.009
Twist	0.000	-0.229	-0.152	0.000	0.009	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.378	0.000	0.000	-0.080	0.000	0.000
Slide	0.000	2.671	1.206	0.000	-0.086	-0.148
Rise	0.000	1.206	8.231	0.000	-0.049	-0.201
Tilt	-0.080	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.086	-0.049	0.000	0.025	0.010
Twist	0.000	-0.148	-0.201	0.000	0.010	0.028

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.13.cte	0.000	0.000

	Mean	Stdev
-aatt.17.cte	0.000	0.000

	Mean	Stdev
-acgt.14.cte	0.000	0.000

	Mean	Stdev
-agct.18.cte	0.000	0.000

	Mean	Stdev
-atat.2.cte	0.000	0.000

	Mean	Stdev
-catg.12.cte	0.000	0.000

	Mean	Stdev
-ccgg.11.cte	0.000	0.000

	Mean	Stdev
-ccgg.4.cte	0.000	0.000

	Mean	Stdev
-ccgg.5.cte	0.000	0.000

	Mean	Stdev
-cgcg.15.cte	0.000	0.000

	Mean	Stdev
-cgcg.6.cte	0.000	0.000

	Mean	Stdev
-gatc.16.cte	0.000	0.000

	Mean	Stdev
-ggcc.19.cte	0.000	0.000

	Mean	Stdev
-gtac.7.cte	0.000	0.000

	Mean	Stdev
-tcga.10.cte	0.000	0.000

	Mean	Stdev
-tcga.3.cte	0.000	0.000

	Mean	Stdev
-ttaa.8.cte	0.000	0.000

	Mean	Stdev
-ttaa.9.cte	0.000	0.000

	Mean	Stdev
rise-10-TCGA	3.353	0.306

	Mean	Stdev
roll-10-TCGA	2.551	5.862

	Mean	Stdev
shift-10-TCGA	0.000	0.821

	Mean	Stdev
slide-10-TCGA	-0.161	0.621

	Mean	Stdev
tilt-10-TCGA	0.000	4.679

	Mean	Stdev
twist-10-TCGA	35.448	6.501

	Mean	Stdev
rise-11-CCGG	3.385	0.304

	Mean	Stdev
roll-11-CCGG	3.914	6.110

	Mean	Stdev
shift-11-CCGG	0.000	0.961

	Mean	Stdev
slide-11-CCGG	-0.253	0.703

	Mean	Stdev
tilt-11-CCGG	0.000	4.823

	Mean	Stdev
twist-11-CCGG	34.426	6.155

	Mean	Stdev
rise-12-CATG	3.073	0.311

	Mean	Stdev
roll-12-CATG	6.498	6.624

	Mean	Stdev
shift-12-CATG	0.000	0.984

	Mean	Stdev
slide-12-CATG	0.100	0.572

	Mean	Stdev
tilt-12-CATG	0.000	5.159

	Mean	Stdev
twist-12-CATG	30.748	7.878

	Mean	Stdev
rise-13-AATT	3.244	0.255

	Mean	Stdev
roll-13-AATT	-0.088	5.623

	Mean	Stdev
shift-13-AATT	0.000	1.053

	Mean	Stdev
slide-13-AATT	0.041	0.538

	Mean	Stdev
tilt-13-AATT	0.000	5.451

	Mean	Stdev
twist-13-AATT	36.576	4.823

	Mean	Stdev
rise-14-ACGT	3.414	0.290

	Mean	Stdev
roll-14-ACGT	-1.426	5.284

	Mean	Stdev
shift-14-ACGT	0.000	0.758

	Mean	Stdev
slide-14-ACGT	-0.692	0.446

	Mean	Stdev
tilt-14-ACGT	0.000	3.915

	Mean	Stdev
twist-14-ACGT	32.991	4.628

	Mean	Stdev
rise-15-CGCG	3.319	0.340

	Mean	Stdev
roll-15-CGCG	5.952	6.490

	Mean	Stdev
shift-15-CGCG	0.000	1.145

	Mean	Stdev
slide-15-CGCG	0.242	0.527

	Mean	Stdev
tilt-15-CGCG	0.000	6.747

	Mean	Stdev
twist-15-CGCG	36.132	5.952

	Mean	Stdev
rise-16-GATC	3.311	0.304

	Mean	Stdev
roll-16-GATC	2.604	5.498

	Mean	Stdev
shift-16-GATC	0.000	1.023

	Mean	Stdev
slide-16-GATC	0.121	0.642

	Mean	Stdev
tilt-16-GATC	0.000	5.314

	Mean	Stdev
twist-16-GATC	35.913	5.843

	Mean	Stdev
rise-17-AATT	3.162	0.267

	Mean	Stdev
roll-17-AATT	0.833	5.538

	Mean	Stdev
shift-17-AATT	0.000	0.684

	Mean	Stdev
slide-17-AATT	-0.379	0.535

	Mean	Stdev
tilt-17-AATT	0.000	4.599

	Mean	Stdev
twist-17-AATT	31.998	5.909

	Mean	Stdev
rise-18-AGCT	3.392	0.307

	Mean	Stdev
roll-18-AGCT	2.342	5.319

	Mean	Stdev
shift-18-AGCT	0.000	0.801

	Mean	Stdev
slide-18-AGCT	-0.564	0.572

	Mean	Stdev
tilt-18-AGCT	0.000	4.734

	Mean	Stdev
twist-18-AGCT	35.038	4.996

	Mean	Stdev
rise-19-GGCC	3.649	0.321

	Mean	Stdev
roll-19-GGCC	3.315	5.257

	Mean	Stdev
shift-19-GGCC	0.000	0.631

	Mean	Stdev
slide-19-GGCC	-1.005	0.641

	Mean	Stdev
tilt-19-GGCC	0.000	4.504

	Mean	Stdev
twist-19-GGCC	36.295	4.839

	Mean	Stdev
rise-2-ATAT	3.344	0.271

	Mean	Stdev
roll-2-ATAT	-2.136	4.467

	Mean	Stdev
shift-2-ATAT	0.000	0.571

	Mean	Stdev
slide-2-ATAT	-0.831	0.373

	Mean	Stdev
tilt-2-ATAT	0.000	3.529

	Mean	Stdev
twist-2-ATAT	31.933	3.795

	Mean	Stdev
rise-3-TCGA	3.412	0.286

	Mean	Stdev
roll-3-TCGA	1.369	5.159

	Mean	Stdev
shift-3-TCGA	0.000	0.914

	Mean	Stdev
slide-3-TCGA	-0.107	0.613

	Mean	Stdev
tilt-3-TCGA	0.000	5.065

	Mean	Stdev
twist-3-TCGA	38.726	4.556

	Mean	Stdev
rise-4-CCGG	3.460	0.321

	Mean	Stdev
roll-4-CCGG	4.300	5.921

	Mean	Stdev
shift-4-CCGG	0.000	0.942

	Mean	Stdev
slide-4-CCGG	-0.486	0.721

	Mean	Stdev
tilt-4-CCGG	0.000	4.919

	Mean	Stdev
twist-4-CCGG	32.977	5.055

	Mean	Stdev
rise-5-CCGG	3.291	0.278

	Mean	Stdev
roll-5-CCGG	4.105	5.322

	Mean	Stdev
shift-5-CCGG	0.000	1.077

	Mean	Stdev
slide-5-CCGG	0.066	0.599

	Mean	Stdev
tilt-5-CCGG	0.000	4.917

	Mean	Stdev
twist-5-CCGG	34.942	5.199

	Mean	Stdev
rise-6-CGCG	3.112	0.317

	Mean	Stdev
roll-6-CGCG	5.057	5.968

	Mean	Stdev
shift-6-CGCG	0.000	1.058

	Mean	Stdev
slide-6-CGCG	0.160	0.542

	Mean	Stdev
tilt-6-CGCG	0.000	5.952

	Mean	Stdev
twist-6-CGCG	33.320	7.290

	Mean	Stdev
rise-7-GTAC	3.406	0.269

	Mean	Stdev
roll-7-GTAC	-1.517	5.065

	Mean	Stdev
shift-7-GTAC	0.000	0.717

	Mean	Stdev
slide-7-GTAC	-0.737	0.454

	Mean	Stdev
tilt-7-GTAC	0.000	4.022

	Mean	Stdev
twist-7-GTAC	33.496	4.488

	Mean	Stdev
rise-8-TTAA	3.336	0.271

	Mean	Stdev
roll-8-TTAA	0.039	5.276

	Mean	Stdev
shift-8-TTAA	0.000	0.671

	Mean	Stdev
slide-8-TTAA	-0.364	0.517

	Mean	Stdev
tilt-8-TTAA	0.000	5.018

	Mean	Stdev
twist-8-TTAA	37.615	4.519

	Mean	Stdev
rise-9-TTAA	3.313	0.295

	Mean	Stdev
roll-9-TTAA	1.708	5.335

	Mean	Stdev
shift-9-TTAA	0.000	0.698

	Mean	Stdev
slide-9-TTAA	-0.300	0.564

	Mean	Stdev
tilt-9-TTAA	0.000	4.278

	Mean	Stdev
twist-9-TTAA	33.975	6.001

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3'	T	-	T	-	A	-	G	-	G	-	G	-	C	-	A	-	A	-	A	-	G	-	G	-	T	-	T	-	G	-	C	-	T	-	T	-	C	-	C	-	G	5'
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	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20		21
5'	A	-	A	-	T	-	C	-	C	-	C	-	G	-	T	-	T	-	T	-	C	-	C	-	A	-	A	-	C	-	G	-	A	-	A	-	G	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	T	-	T	-	A	-	G	-	G	-	G	-	C	-	A	-	A	-	A	-	G	-	G	-	T	-	T	-	G	-	C	-	T	-	T	-	C	-	C	-	G	5'
	42		41		40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20		21
5'	A	-	A	-	T	-	C	-	C	-	C	-	G	-	T	-	T	-	T	-	C	-	C	-	A	-	A	-	C	-	G	-	A	-	A	-	G	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	T	-	T	-	A	-	G	-	G	-	G	-	C	-	A	-	A	-	A	-	G	-	G	-	T	-	T	-	G	-	C	-	T	-	T	-	C	-	C	-	G	5'
	42		41		40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22