NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12		13
5'	C	-	C	-	C	-	T	-	A	-	A	-	C	-	C	-	C	-	T	-	A	-	A	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	G	-	G	-	A	-	T	-	T	-	G	-	G	-	G	-	A	-	T	-	T	-	G	5'
	26		25		24		23		22		21		20		19		18		17		16		15		14

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.787	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	2.496	1.074	0.000	-0.056	-0.151
Rise	0.000	1.074	8.968	0.000	-0.028	-0.145
Tilt	-0.095	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.056	-0.028	0.000	0.022	0.008
Twist	0.000	-0.151	-0.145	0.000	0.008	0.042

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.678	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.811	0.882	0.000	-0.082	-0.167
Rise	0.000	0.882	9.900	0.000	-0.035	-0.178
Tilt	-0.084	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.082	-0.035	0.000	0.023	0.010
Twist	0.000	-0.167	-0.178	0.000	0.010	0.038

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.560	0.000	0.000	-0.065	0.000	0.000
Slide	0.000	4.364	3.610	0.000	0.021	-0.125
Rise	0.000	3.610	11.497	0.000	0.106	-0.198
Tilt	-0.065	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.021	0.106	0.000	0.030	0.008
Twist	0.000	-0.125	-0.198	0.000	0.008	0.040

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.142	0.000	0.000	-0.103	0.000	0.000
Slide	0.000	1.714	1.761	0.000	0.002	-0.116
Rise	0.000	1.761	7.609	0.000	-0.007	-0.163
Tilt	-0.103	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.002	-0.007	0.000	0.019	0.003
Twist	0.000	-0.116	-0.163	0.000	0.003	0.031

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.797	0.000	0.000	-0.118	0.000	0.000
Slide	0.000	2.161	2.070	0.000	-0.005	-0.150
Rise	0.000	2.070	7.904	0.000	-0.051	-0.174
Tilt	-0.118	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.005	-0.051	0.000	0.023	0.005
Twist	0.000	-0.150	-0.174	0.000	0.005	0.038

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.288	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	1.728	1.791	0.000	-0.001	-0.106
Rise	0.000	1.791	8.167	0.000	-0.021	-0.177
Tilt	-0.106	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.001	-0.021	0.000	0.022	0.004
Twist	0.000	-0.106	-0.177	0.000	0.004	0.029

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.753	0.000	0.000	-0.045	0.000	0.000
Slide	0.000	2.334	1.500	0.000	-0.050	-0.113
Rise	0.000	1.500	9.753	0.000	-0.033	-0.185
Tilt	-0.045	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.050	-0.033	0.000	0.021	0.006
Twist	0.000	-0.113	-0.185	0.000	0.006	0.023

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.712	0.000	0.000	-0.051	0.000	0.000
Slide	0.000	2.445	1.650	0.000	-0.050	-0.118
Rise	0.000	1.650	9.831	0.000	-0.038	-0.200
Tilt	-0.051	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.050	-0.038	0.000	0.019	0.005
Twist	0.000	-0.118	-0.200	0.000	0.005	0.025

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.417	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	2.923	0.712	0.000	0.015	-0.164
Rise	0.000	0.712	8.706	0.000	-0.132	-0.254
Tilt	-0.054	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.015	-0.132	0.000	0.020	0.014
Twist	0.000	-0.164	-0.254	0.000	0.014	0.032

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.404	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	2.643	0.559	0.000	0.012	-0.149
Rise	0.000	0.559	9.074	0.000	-0.117	-0.221
Tilt	-0.048	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.012	-0.117	0.000	0.019	0.013
Twist	0.000	-0.149	-0.221	0.000	0.013	0.028

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-aatt.11.cte	0.000	0.000

	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-acgt.6.cte	0.000	0.000

	Mean	Stdev
-ccgg.2.cte	0.000	0.000

	Mean	Stdev
-ccgg.7.cte	0.000	0.000

	Mean	Stdev
-ccgg.8.cte	0.000	0.000

	Mean	Stdev
-ctag.3.cte	0.000	0.000

	Mean	Stdev
-ctag.9.cte	0.000	0.000

	Mean	Stdev
-tata.10.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
rise-10-TATA	3.098	0.301

	Mean	Stdev
roll-10-TATA	4.974	7.671

	Mean	Stdev
shift-10-TATA	0.000	1.395

	Mean	Stdev
slide-10-TATA	0.258	0.628

	Mean	Stdev
tilt-10-TATA	0.000	5.737

	Mean	Stdev
twist-10-TATA	33.630	7.760

	Mean	Stdev
rise-11-AATT	3.250	0.266

	Mean	Stdev
roll-11-AATT	0.112	5.403

	Mean	Stdev
shift-11-AATT	0.000	1.083

	Mean	Stdev
slide-11-AATT	0.037	0.558

	Mean	Stdev
tilt-11-AATT	0.000	5.404

	Mean	Stdev
twist-11-AATT	36.333	4.344

	Mean	Stdev
rise-2-CCGG	3.521	0.320

	Mean	Stdev
roll-2-CCGG	3.245	5.616

	Mean	Stdev
shift-2-CCGG	0.000	0.832

	Mean	Stdev
slide-2-CCGG	-0.525	0.739

	Mean	Stdev
tilt-2-CCGG	0.000	4.694

	Mean	Stdev
twist-2-CCGG	35.171	5.170

	Mean	Stdev
rise-3-CTAG	3.138	0.270

	Mean	Stdev
roll-3-CTAG	1.413	5.571

	Mean	Stdev
shift-3-CTAG	0.000	0.924

	Mean	Stdev
slide-3-CTAG	-0.288	0.585

	Mean	Stdev
tilt-3-CTAG	0.000	4.659

	Mean	Stdev
twist-3-CTAG	31.246	6.077

	Mean	Stdev
rise-4-TATA	3.064	0.287

	Mean	Stdev
roll-4-TATA	4.653	7.496

	Mean	Stdev
shift-4-TATA	0.000	1.385

	Mean	Stdev
slide-4-TATA	0.295	0.655

	Mean	Stdev
tilt-4-TATA	0.000	5.700

	Mean	Stdev
twist-4-TATA	33.779	7.963

	Mean	Stdev
rise-5-AATT	3.193	0.255

	Mean	Stdev
roll-5-AATT	1.150	5.472

	Mean	Stdev
shift-5-AATT	0.000	1.123

	Mean	Stdev
slide-5-AATT	0.062	0.540

	Mean	Stdev
tilt-5-AATT	0.000	5.073

	Mean	Stdev
twist-5-AATT	35.537	4.866

	Mean	Stdev
rise-6-ACGT	3.386	0.273

	Mean	Stdev
roll-6-ACGT	-0.848	4.656

	Mean	Stdev
shift-6-ACGT	0.000	0.633

	Mean	Stdev
slide-6-ACGT	-0.806	0.433

	Mean	Stdev
tilt-6-ACGT	0.000	3.835

	Mean	Stdev
twist-6-ACGT	33.547	4.220

	Mean	Stdev
rise-7-CCGG	3.647	0.318

	Mean	Stdev
roll-7-CCGG	4.843	5.174

	Mean	Stdev
shift-7-CCGG	0.000	0.638

	Mean	Stdev
slide-7-CCGG	-1.004	0.676

	Mean	Stdev
tilt-7-CCGG	0.000	4.325

	Mean	Stdev
twist-7-CCGG	35.906	4.671

	Mean	Stdev
rise-8-CCGG	3.488	0.310

	Mean	Stdev
roll-8-CCGG	3.539	5.232

	Mean	Stdev
shift-8-CCGG	0.000	0.768

	Mean	Stdev
slide-8-CCGG	-0.670	0.714

	Mean	Stdev
tilt-8-CCGG	0.000	4.469

	Mean	Stdev
twist-8-CCGG	34.711	5.224

	Mean	Stdev
rise-9-CTAG	3.152	0.272

	Mean	Stdev
roll-9-CTAG	1.804	5.796

	Mean	Stdev
shift-9-CTAG	0.000	0.968

	Mean	Stdev
slide-9-CTAG	-0.284	0.574

	Mean	Stdev
tilt-9-CTAG	0.000	4.727

	Mean	Stdev
twist-9-CTAG	31.957	5.914

	1		2		3		4		5		6		7		8		9		10		11		12		13
5'	C	-	C	-	C	-	T	-	A	-	A	-	C	-	C	-	C	-	T	-	A	-	A	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	G	-	G	-	A	-	T	-	T	-	G	-	G	-	G	-	A	-	T	-	T	-	G	5'
	26		25		24		23		22		21		20		19		18		17		16		15		14

	1		2		3		4		5		6		7		8		9		10		11		12		13
5'	C	-	C	-	C	-	T	-	A	-	A	-	C	-	C	-	C	-	T	-	A	-	A	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	G	-	G	-	A	-	T	-	T	-	G	-	G	-	G	-	A	-	T	-	T	-	G	5'
	26		25		24		23		22		21		20		19		18		17		16		15		14

	1		2		3		4		5		6		7		8		9		10		11		12		13
5'	C	-	C	-	C	-	T	-	A	-	A	-	C	-	C	-	C	-	T	-	A	-	A	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	G	-	G	-	A	-	T	-	T	-	G	-	G	-	G	-	A	-	T	-	T	-	G	5'
	26		25		24		23		22		21		20		19		18		17		16		15		14