NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.738	0.675	0.530	-0.032	0.004	0.054
Slide	0.675	2.872	1.400	-0.010	-0.077	-0.145
Rise	0.530	1.400	8.958	-0.254	-0.019	-0.202
Tilt	-0.032	-0.010	-0.254	0.047	0.001	0.008
Roll	0.004	-0.077	-0.019	0.001	0.022	0.007
Twist	0.054	-0.145	-0.202	0.008	0.007	0.032

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.011	0.382	0.600	-0.039	0.020	0.048
Slide	0.382	2.927	1.090	0.018	-0.067	-0.149
Rise	0.600	1.090	9.087	-0.217	-0.075	-0.176
Tilt	-0.039	0.018	-0.217	0.041	0.001	0.004
Roll	0.020	-0.067	-0.075	0.001	0.023	0.011
Twist	0.048	-0.149	-0.176	0.004	0.011	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.234	-0.542	0.378	-0.035	0.041	0.062
Slide	-0.542	3.946	2.874	-0.015	0.029	-0.145
Rise	0.378	2.874	11.029	0.036	0.117	-0.209
Tilt	-0.035	-0.015	0.036	0.045	0.005	0.005
Roll	0.041	0.029	0.117	0.005	0.022	0.005
Twist	0.062	-0.145	-0.209	0.005	0.005	0.043

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.443	0.159	0.324	-0.041	0.014	0.059
Slide	0.159	3.224	2.613	-0.011	0.007	-0.064
Rise	0.324	2.613	10.564	0.035	0.109	-0.128
Tilt	-0.041	-0.011	0.035	0.044	0.006	0.004
Roll	0.014	0.007	0.109	0.006	0.026	0.008
Twist	0.059	-0.064	-0.128	0.004	0.008	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.811	0.038	0.453	-0.045	0.011	0.019
Slide	0.038	3.628	2.923	-0.035	0.039	-0.091
Rise	0.453	2.923	11.047	0.023	0.116	-0.178
Tilt	-0.045	-0.035	0.023	0.043	0.005	0.008
Roll	0.011	0.039	0.116	0.005	0.021	0.006
Twist	0.019	-0.091	-0.178	0.008	0.006	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.838	0.045	0.005	-0.073	0.016	-0.030
Slide	0.045	2.132	1.185	-0.015	0.017	-0.095
Rise	0.005	1.185	6.849	0.010	-0.088	-0.224
Tilt	-0.073	-0.015	0.010	0.027	-0.001	0.002
Roll	0.016	0.017	-0.088	-0.001	0.017	0.008
Twist	-0.030	-0.095	-0.224	0.002	0.008	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.843	0.129	0.317	-0.077	0.004	-0.020
Slide	0.129	2.659	0.668	-0.011	0.018	-0.097
Rise	0.317	0.668	7.687	0.012	-0.092	-0.205
Tilt	-0.077	-0.011	0.012	0.030	-0.002	0.001
Roll	0.004	0.018	-0.092	-0.002	0.018	0.009
Twist	-0.020	-0.097	-0.205	0.001	0.009	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.951	-0.179	0.318	-0.072	0.001	-0.023
Slide	-0.179	2.054	1.282	-0.002	0.009	-0.092
Rise	0.318	1.282	6.578	-0.033	-0.082	-0.214
Tilt	-0.072	-0.002	-0.033	0.026	-0.001	0.002
Roll	0.001	0.009	-0.082	-0.001	0.016	0.008
Twist	-0.023	-0.092	-0.214	0.002	0.008	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.636	-0.775	-0.501	-0.062	-0.041	-0.063
Slide	-0.775	2.669	1.963	0.026	-0.008	-0.123
Rise	-0.501	1.963	9.300	0.245	0.016	-0.238
Tilt	-0.062	0.026	0.245	0.049	-0.000	-0.012
Roll	-0.041	-0.008	0.016	-0.000	0.022	0.007
Twist	-0.063	-0.123	-0.238	-0.012	0.007	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.091	-0.002	0.157	-0.124	0.008	0.027
Slide	-0.002	3.531	2.436	0.002	0.077	-0.117
Rise	0.157	2.436	10.964	-0.004	0.143	-0.220
Tilt	-0.124	0.002	-0.004	0.044	0.000	-0.000
Roll	0.008	0.077	0.143	0.000	0.023	0.004
Twist	0.027	-0.117	-0.220	-0.000	0.004	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.814	0.252	-0.474	-0.058	0.002	0.018
Slide	0.252	2.846	0.324	0.024	0.019	-0.117
Rise	-0.474	0.324	8.856	0.017	-0.113	-0.228
Tilt	-0.058	0.024	0.017	0.029	0.002	-0.000
Roll	0.002	0.019	-0.113	0.002	0.020	0.011
Twist	0.018	-0.117	-0.228	-0.000	0.011	0.026

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-aatt.9.cte	0.000	0.000

	Mean	Stdev
-acgt.10.cte	0.000	0.000

	Mean	Stdev
-acgt.12.cte	0.000	0.000

	Mean	Stdev
-acgt.6.cte	0.000	0.000

	Mean	Stdev
-catg.11.cte	0.000	0.000

	Mean	Stdev
-catg.5.cte	0.000	0.000

	Mean	Stdev
-catg.7.cte	0.000	0.000

	Mean	Stdev
-ctag.2.cte	0.000	0.000

	Mean	Stdev
-gcgc.4.cte	0.000	0.000

	Mean	Stdev
-tgca.3.cte	0.000	0.000

	Mean	Stdev
rise-10-ACGT	3.342	0.280

	Mean	Stdev
roll-10-ACGT	-2.395	5.686

	Mean	Stdev
shift-10-ACGT	0.679	0.799

	Mean	Stdev
slide-10-ACGT	-0.641	0.466

	Mean	Stdev
tilt-10-ACGT	0.416	3.800

	Mean	Stdev
twist-10-ACGT	31.848	4.285

	Mean	Stdev
rise-11-CATG	3.305	0.354

	Mean	Stdev
roll-11-CATG	7.796	6.883

	Mean	Stdev
shift-11-CATG	-0.240	1.022

	Mean	Stdev
slide-11-CATG	-0.157	0.614

	Mean	Stdev
tilt-11-CATG	0.653	5.422

	Mean	Stdev
twist-11-CATG	34.664	6.846

	Mean	Stdev
rise-12-ACGT	3.331	0.282

	Mean	Stdev
roll-12-ACGT	-1.028	5.263

	Mean	Stdev
shift-12-ACGT	0.127	0.695

	Mean	Stdev
slide-12-ACGT	-0.634	0.483

	Mean	Stdev
tilt-12-ACGT	-0.612	3.806

	Mean	Stdev
twist-12-ACGT	30.471	5.337

	Mean	Stdev
rise-2-CTAG	3.342	0.302

	Mean	Stdev
roll-2-CTAG	0.876	5.383

	Mean	Stdev
shift-2-CTAG	0.492	0.782

	Mean	Stdev
slide-2-CTAG	-0.314	0.627

	Mean	Stdev
tilt-2-CTAG	3.150	4.054

	Mean	Stdev
twist-2-CTAG	35.235	5.048

	Mean	Stdev
rise-3-TGCA	3.028	0.298

	Mean	Stdev
roll-3-TGCA	6.988	6.713

	Mean	Stdev
shift-3-TGCA	-0.121	0.985

	Mean	Stdev
slide-3-TGCA	-0.096	0.577

	Mean	Stdev
tilt-3-TGCA	-0.776	4.995

	Mean	Stdev
twist-3-TGCA	30.443	7.218

	Mean	Stdev
rise-4-GCGC	3.408	0.272

	Mean	Stdev
roll-4-GCGC	-2.075	5.667

	Mean	Stdev
shift-4-GCGC	0.226	0.908

	Mean	Stdev
slide-4-GCGC	-0.219	0.470

	Mean	Stdev
tilt-4-GCGC	0.777	4.456

	Mean	Stdev
twist-4-GCGC	35.376	4.662

	Mean	Stdev
rise-5-CATG	3.190	0.314

	Mean	Stdev
roll-5-CATG	7.106	6.700

	Mean	Stdev
shift-5-CATG	-0.377	0.972

	Mean	Stdev
slide-5-CATG	-0.227	0.527

	Mean	Stdev
tilt-5-CATG	-0.135	5.164

	Mean	Stdev
twist-5-CATG	33.006	6.349

	Mean	Stdev
rise-6-ACGT	3.310	0.286

	Mean	Stdev
roll-6-ACGT	-1.662	5.930

	Mean	Stdev
shift-6-ACGT	0.302	0.918

	Mean	Stdev
slide-6-ACGT	-0.549	0.464

	Mean	Stdev
tilt-6-ACGT	-0.763	4.032

	Mean	Stdev
twist-6-ACGT	31.634	5.130

	Mean	Stdev
rise-7-CATG	3.336	0.365

	Mean	Stdev
roll-7-CATG	8.762	7.037

	Mean	Stdev
shift-7-CATG	-0.148	0.927

	Mean	Stdev
slide-7-CATG	-0.204	0.645

	Mean	Stdev
tilt-7-CATG	1.722	5.359

	Mean	Stdev
twist-7-CATG	33.066	7.128

	Mean	Stdev
rise-8-AATT	3.345	0.302

	Mean	Stdev
roll-8-AATT	1.007	5.457

	Mean	Stdev
shift-8-AATT	-0.387	0.709

	Mean	Stdev
slide-8-AATT	-0.238	0.613

	Mean	Stdev
tilt-8-AATT	-2.412	3.970

	Mean	Stdev
twist-8-AATT	34.224	5.900

	Mean	Stdev
rise-9-AATT	3.315	0.288

	Mean	Stdev
roll-9-AATT	0.757	5.437

	Mean	Stdev
shift-9-AATT	-0.225	0.583

	Mean	Stdev
slide-9-AATT	-0.461	0.527

	Mean	Stdev
tilt-9-AATT	-1.918	4.115

	Mean	Stdev
twist-9-AATT	36.401	4.649

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