NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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3'	G	-	C	-	A	-	T	-	G	-	G	-	G	-	T	-	A	-	A	-	T	-	T	-	A	-	C	-	C	-	C	-	A	-	T	-	G	-	C	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.867	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	2.837	1.231	0.000	-0.072	-0.180
Rise	0.000	1.231	9.099	0.000	-0.035	-0.151
Tilt	-0.090	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.072	-0.035	0.000	0.020	0.007
Twist	0.000	-0.180	-0.151	0.000	0.007	0.037

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.889	0.000	0.000	-0.030	0.000	0.000
Slide	0.000	4.019	2.616	0.000	0.003	-0.125
Rise	0.000	2.616	11.307	0.000	0.103	-0.219
Tilt	-0.030	0.000	0.000	0.045	0.000	0.000
Roll	0.000	0.003	0.103	0.000	0.023	0.006
Twist	0.000	-0.125	-0.219	0.000	0.006	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.067	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	4.365	3.448	0.000	0.021	-0.128
Rise	0.000	3.448	11.874	0.000	0.113	-0.227
Tilt	-0.048	0.000	0.000	0.044	0.000	0.000
Roll	0.000	0.021	0.113	0.000	0.026	0.004
Twist	0.000	-0.128	-0.227	0.000	0.004	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.852	0.000	0.000	-0.009	0.000	0.000
Slide	0.000	4.906	3.013	0.000	-0.047	-0.140
Rise	0.000	3.013	11.620	0.000	0.058	-0.136
Tilt	-0.009	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.047	0.058	0.000	0.032	0.014
Twist	0.000	-0.140	-0.136	0.000	0.014	0.058

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.773	0.000	0.000	-0.017	0.000	0.000
Slide	0.000	5.161	3.280	0.000	-0.052	-0.146
Rise	0.000	3.280	12.273	0.000	0.051	-0.175
Tilt	-0.017	0.000	0.000	0.046	0.000	0.000
Roll	0.000	-0.052	0.051	0.000	0.031	0.012
Twist	0.000	-0.146	-0.175	0.000	0.012	0.057

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.582	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	3.117	0.687	0.000	0.020	-0.126
Rise	0.000	0.687	8.502	0.000	-0.065	-0.216
Tilt	-0.094	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.020	-0.065	0.000	0.018	0.008
Twist	0.000	-0.126	-0.216	0.000	0.008	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.230	0.000	0.000	-0.087	0.000	0.000
Slide	0.000	2.710	1.802	0.000	-0.023	-0.144
Rise	0.000	1.802	7.343	0.000	-0.023	-0.132
Tilt	-0.087	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.023	-0.023	0.000	0.022	0.007
Twist	0.000	-0.144	-0.132	0.000	0.007	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.915	0.000	0.000	-0.107	0.000	0.000
Slide	0.000	2.280	1.882	0.000	-0.035	-0.155
Rise	0.000	1.882	9.527	0.000	-0.007	-0.153
Tilt	-0.107	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.035	-0.007	0.000	0.024	0.006
Twist	0.000	-0.155	-0.153	0.000	0.006	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.848	0.000	0.000	-0.110	0.000	0.000
Slide	0.000	2.204	1.819	0.000	-0.039	-0.150
Rise	0.000	1.819	9.290	0.000	-0.005	-0.157
Tilt	-0.110	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.039	-0.005	0.000	0.022	0.006
Twist	0.000	-0.150	-0.157	0.000	0.006	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.871	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	2.006	1.894	0.000	-0.013	-0.105
Rise	0.000	1.894	7.488	0.000	-0.045	-0.155
Tilt	-0.095	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.013	-0.045	0.000	0.022	0.008
Twist	0.000	-0.105	-0.155	0.000	0.008	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.974	0.000	0.000	-0.046	0.000	0.000
Slide	0.000	4.212	3.349	0.000	0.028	-0.121
Rise	0.000	3.349	11.273	0.000	0.112	-0.222
Tilt	-0.046	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.028	0.112	0.000	0.025	0.004
Twist	0.000	-0.121	-0.222	0.000	0.004	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.714	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	3.956	2.561	0.000	0.027	-0.111
Rise	0.000	2.561	10.510	0.000	0.111	-0.191
Tilt	-0.026	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.027	0.111	0.000	0.025	0.011
Twist	0.000	-0.111	-0.191	0.000	0.011	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.514	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	2.542	1.134	0.000	0.016	-0.136
Rise	0.000	1.133	7.941	0.000	-0.133	-0.236
Tilt	-0.043	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.016	-0.133	0.000	0.020	0.015
Twist	0.000	-0.136	-0.236	0.000	0.015	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.415	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	2.843	0.796	0.000	0.019	-0.152
Rise	0.000	0.796	8.849	0.000	-0.118	-0.253
Tilt	-0.053	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.019	-0.118	0.000	0.019	0.012
Twist	0.000	-0.152	-0.253	0.000	0.012	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.374	0.000	0.000	-0.052	0.000	0.000
Slide	0.000	2.841	0.602	0.000	0.026	-0.147
Rise	0.000	0.602	8.744	0.000	-0.106	-0.222
Tilt	-0.052	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.026	-0.106	0.000	0.020	0.012
Twist	0.000	-0.147	-0.222	0.000	0.012	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.558	0.000	0.000	-0.086	0.000	0.000
Slide	0.000	2.957	0.490	0.000	0.026	-0.110
Rise	0.000	0.491	8.342	0.000	-0.084	-0.207
Tilt	-0.086	0.000	0.000	0.031	0.000	0.000
Roll	0.000	0.026	-0.084	0.000	0.018	0.008
Twist	0.000	-0.110	-0.207	0.000	0.008	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.845	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	2.710	1.415	0.000	-0.065	-0.159
Rise	0.000	1.415	9.258	0.000	-0.050	-0.162
Tilt	-0.094	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.065	-0.050	0.000	0.021	0.010
Twist	0.000	-0.159	-0.162	0.000	0.010	0.045

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.11.cte	0.000	0.000

	Mean	Stdev
-acgt.18.cte	0.000	0.000

	Mean	Stdev
-acgt.4.cte	0.000	0.000

	Mean	Stdev
-atat.12.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-catg.7.cte	0.000	0.000

	Mean	Stdev
-ccgg.5.cte	0.000	0.000

	Mean	Stdev
-ccgg.6.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.15.cte	0.000	0.000

	Mean	Stdev
-gtac.16.cte	0.000	0.000

	Mean	Stdev
-gtac.2.cte	0.000	0.000

	Mean	Stdev
-tata.10.cte	0.000	0.000

	Mean	Stdev
-tata.17.cte	0.000	0.000

	Mean	Stdev
-tata.3.cte	0.000	0.000

	Mean	Stdev
-tgca.13.cte	0.000	0.000

	Mean	Stdev
-ttaa.9.cte	0.000	0.000

	Mean	Stdev
rise-10-TATA	3.246	0.312

	Mean	Stdev
roll-10-TATA	8.781	7.870

	Mean	Stdev
shift-10-TATA	0.000	1.144

	Mean	Stdev
slide-10-TATA	-0.126	0.649

	Mean	Stdev
tilt-10-TATA	0.000	4.964

	Mean	Stdev
twist-10-TATA	30.400	7.693

	Mean	Stdev
rise-11-AATT	3.287	0.265

	Mean	Stdev
roll-11-AATT	0.030	5.675

	Mean	Stdev
shift-11-AATT	0.000	1.001

	Mean	Stdev
slide-11-AATT	-0.171	0.561

	Mean	Stdev
tilt-11-AATT	0.000	5.502

	Mean	Stdev
twist-11-AATT	36.885	4.905

	Mean	Stdev
rise-12-ATAT	3.308	0.250

	Mean	Stdev
roll-12-ATAT	-2.653	4.598

	Mean	Stdev
shift-12-ATAT	0.000	0.563

	Mean	Stdev
slide-12-ATAT	-0.901	0.388

	Mean	Stdev
tilt-12-ATAT	0.000	3.609

	Mean	Stdev
twist-12-ATAT	32.133	3.489

	Mean	Stdev
rise-13-TGCA	3.208	0.291

	Mean	Stdev
roll-13-TGCA	5.620	6.288

	Mean	Stdev
shift-13-TGCA	0.000	1.360

	Mean	Stdev
slide-13-TGCA	0.151	0.496

	Mean	Stdev
tilt-13-TGCA	0.000	5.782

	Mean	Stdev
twist-13-TGCA	34.797	5.412

	Mean	Stdev
rise-14-GGCC	3.316	0.276

	Mean	Stdev
roll-14-GGCC	3.647	5.326

	Mean	Stdev
shift-14-GGCC	0.000	1.012

	Mean	Stdev
slide-14-GGCC	-0.215	0.679

	Mean	Stdev
tilt-14-GGCC	0.000	4.450

	Mean	Stdev
twist-14-GGCC	32.350	5.917

	Mean	Stdev
rise-15-GGCC	3.659	0.324

	Mean	Stdev
roll-15-GGCC	3.668	5.435

	Mean	Stdev
shift-15-GGCC	0.000	0.603

	Mean	Stdev
slide-15-GGCC	-1.025	0.651

	Mean	Stdev
tilt-15-GGCC	0.000	4.405

	Mean	Stdev
twist-15-GGCC	37.086	4.681

	Mean	Stdev
rise-16-GTAC	3.281	0.282

	Mean	Stdev
roll-16-GTAC	-1.142	5.185

	Mean	Stdev
shift-16-GTAC	0.000	0.796

	Mean	Stdev
slide-16-GTAC	-0.532	0.436

	Mean	Stdev
tilt-16-GTAC	0.000	3.775

	Mean	Stdev
twist-16-GTAC	32.183	5.317

	Mean	Stdev
rise-17-TATA	3.109	0.297

	Mean	Stdev
roll-17-TATA	4.365	7.291

	Mean	Stdev
shift-17-TATA	0.000	1.386

	Mean	Stdev
slide-17-TATA	0.167	0.603

	Mean	Stdev
tilt-17-TATA	0.000	5.610

	Mean	Stdev
twist-17-TATA	34.913	7.276

	Mean	Stdev
rise-18-ACGT	3.259	0.271

	Mean	Stdev
roll-18-ACGT	-1.878	5.483

	Mean	Stdev
shift-18-ACGT	0.000	0.821

	Mean	Stdev
slide-18-ACGT	-0.444	0.429

	Mean	Stdev
tilt-18-ACGT	0.000	3.670

	Mean	Stdev
twist-18-ACGT	30.280	5.374

	Mean	Stdev
rise-2-GTAC	3.276	0.279

	Mean	Stdev
roll-2-GTAC	-1.912	5.590

	Mean	Stdev
shift-2-GTAC	0.000	0.917

	Mean	Stdev
slide-2-GTAC	-0.388	0.430

	Mean	Stdev
tilt-2-GTAC	0.000	3.793

	Mean	Stdev
twist-2-GTAC	31.758	4.841

	Mean	Stdev
rise-3-TATA	3.098	0.288

	Mean	Stdev
roll-3-TATA	4.240	7.054

	Mean	Stdev
shift-3-TATA	0.000	1.480

	Mean	Stdev
slide-3-TATA	0.158	0.590

	Mean	Stdev
tilt-3-TATA	0.000	5.651

	Mean	Stdev
twist-3-TATA	34.562	6.761

	Mean	Stdev
rise-4-ACGT	3.263	0.271

	Mean	Stdev
roll-4-ACGT	-1.219	5.016

	Mean	Stdev
shift-4-ACGT	0.000	0.760

	Mean	Stdev
slide-4-ACGT	-0.517	0.428

	Mean	Stdev
tilt-4-ACGT	0.000	3.735

	Mean	Stdev
twist-4-ACGT	31.766	5.116

	Mean	Stdev
rise-5-CCGG	3.703	0.310

	Mean	Stdev
roll-5-CCGG	3.334	5.353

	Mean	Stdev
shift-5-CCGG	0.000	0.540

	Mean	Stdev
slide-5-CCGG	-1.191	0.553

	Mean	Stdev
tilt-5-CCGG	0.000	4.313

	Mean	Stdev
twist-5-CCGG	37.492	4.536

	Mean	Stdev
rise-6-CCGG	3.321	0.272

	Mean	Stdev
roll-6-CCGG	3.564	5.088

	Mean	Stdev
shift-6-CCGG	0.000	0.960

	Mean	Stdev
slide-6-CCGG	-0.248	0.682

	Mean	Stdev
tilt-6-CCGG	0.000	4.550

	Mean	Stdev
twist-6-CCGG	32.115	6.178

	Mean	Stdev
rise-7-CATG	3.205	0.288

	Mean	Stdev
roll-7-CATG	5.494	6.179

	Mean	Stdev
shift-7-CATG	0.000	1.384

	Mean	Stdev
slide-7-CATG	0.167	0.486

	Mean	Stdev
tilt-7-CATG	0.000	5.903

	Mean	Stdev
twist-7-CATG	34.512	5.320

	Mean	Stdev
rise-8-ATAT	3.306	0.246

	Mean	Stdev
roll-8-ATAT	-2.424	4.601

	Mean	Stdev
shift-8-ATAT	0.000	0.576

	Mean	Stdev
slide-8-ATAT	-0.871	0.381

	Mean	Stdev
tilt-8-ATAT	0.000	3.562

	Mean	Stdev
twist-8-ATAT	31.996	3.485

	Mean	Stdev
rise-9-TTAA	3.288	0.265

	Mean	Stdev
roll-9-TTAA	0.472	5.687

	Mean	Stdev
shift-9-TTAA	0.000	1.039

	Mean	Stdev
slide-9-TTAA	-0.103	0.538

	Mean	Stdev
tilt-9-TTAA	0.000	5.591

	Mean	Stdev
twist-9-TTAA	37.594	4.205

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5'	C	-	G	-	T	-	A	-	C	-	C	-	C	-	A	-	T	-	T	-	A	-	A	-	T	-	G	-	G	-	G	-	T	-	A	-	C	-	G	3'
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	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20
5'	C	-	G	-	T	-	A	-	C	-	C	-	C	-	A	-	T	-	T	-	A	-	A	-	T	-	G	-	G	-	G	-	T	-	A	-	C	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	C	-	A	-	T	-	G	-	G	-	G	-	T	-	A	-	A	-	T	-	T	-	A	-	C	-	C	-	C	-	A	-	T	-	G	-	C	5'
	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21