NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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5'	C	-	A	-	C	-	A	-	G	-	G	-	A	-	T	-	G	-	T	-	C	-	C	-	A	-	T	-	A	-	T	-	T	-	A	-	G	-	G	-	A	-	C	-	A	3'
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3'	G	-	T	-	G	-	T	-	C	-	C	-	T	-	A	-	C	-	A	-	G	-	G	-	T	-	A	-	T	-	A	-	A	-	T	-	C	-	C	-	T	-	G	-	T	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.651	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	4.091	2.913	0.000	0.045	-0.082
Rise	0.000	2.912	10.868	0.000	0.086	-0.227
Tilt	-0.026	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.045	0.086	0.000	0.022	0.008
Twist	0.000	-0.082	-0.227	0.000	0.008	0.034

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.938	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	3.918	2.972	0.000	0.035	-0.036
Rise	0.000	2.972	10.002	0.000	0.085	-0.175
Tilt	-0.029	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.035	0.085	0.000	0.026	0.008
Twist	0.000	-0.036	-0.175	0.000	0.008	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.769	0.000	0.000	-0.042	0.000	0.000
Slide	0.000	2.365	1.765	0.000	-0.071	-0.145
Rise	0.000	1.765	8.942	0.000	-0.020	-0.160
Tilt	-0.042	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.071	-0.020	0.000	0.020	0.005
Twist	0.000	-0.145	-0.160	0.000	0.005	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.008	0.000	0.000	-0.093	0.000	0.000
Slide	0.000	2.180	1.622	0.000	-0.054	-0.105
Rise	0.000	1.622	9.377	0.000	-0.019	-0.205
Tilt	-0.093	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.054	-0.019	0.000	0.022	0.006
Twist	0.000	-0.105	-0.205	0.000	0.006	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.311	0.000	0.000	0.025	0.000	0.000
Slide	0.000	4.802	3.323	0.000	-0.090	-0.072
Rise	0.000	3.323	12.117	0.000	0.044	-0.161
Tilt	0.025	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.090	0.044	0.000	0.030	0.014
Twist	0.000	-0.072	-0.161	0.000	0.014	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.764	0.000	0.000	-0.017	0.000	0.000
Slide	0.000	4.788	2.972	0.000	-0.030	-0.129
Rise	0.000	2.972	11.070	0.000	0.049	-0.161
Tilt	-0.017	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.030	0.049	0.000	0.033	0.009
Twist	0.000	-0.129	-0.161	0.000	0.009	0.055

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.913	0.000	0.000	-0.013	0.000	0.000
Slide	0.000	5.102	3.387	0.000	-0.064	-0.077
Rise	0.000	3.387	12.155	0.000	0.074	-0.169
Tilt	-0.013	0.000	0.000	0.048	0.000	0.000
Roll	0.000	-0.064	0.074	0.000	0.030	0.013
Twist	0.000	-0.077	-0.169	0.000	0.013	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.645	0.000	0.000	-0.073	0.000	0.000
Slide	0.000	2.389	1.027	0.000	0.011	-0.112
Rise	0.000	1.027	7.734	0.000	-0.082	-0.217
Tilt	-0.073	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.011	-0.082	0.000	0.017	0.008
Twist	0.000	-0.112	-0.217	0.000	0.008	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.645	0.000	0.000	-0.073	0.000	0.000
Slide	0.000	2.513	0.783	0.000	0.024	-0.105
Rise	0.000	0.782	8.366	0.000	-0.088	-0.229
Tilt	-0.073	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.024	-0.088	0.000	0.017	0.009
Twist	0.000	-0.105	-0.229	0.000	0.009	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.824	0.000	0.000	-0.101	0.000	0.000
Slide	0.000	1.883	1.746	0.000	-0.013	-0.087
Rise	0.000	1.746	8.410	0.000	-0.046	-0.194
Tilt	-0.101	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.013	-0.046	0.000	0.019	0.008
Twist	0.000	-0.087	-0.194	0.000	0.008	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.094	0.000	0.000	-0.093	0.000	0.000
Slide	0.000	1.938	1.167	0.000	-0.036	-0.100
Rise	0.000	1.167	8.163	0.000	-0.039	-0.165
Tilt	-0.093	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.036	-0.039	0.000	0.021	0.008
Twist	0.000	-0.100	-0.165	0.000	0.008	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.265	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.083	1.350	0.000	-0.026	-0.129
Rise	0.000	1.350	8.104	0.000	-0.046	-0.145
Tilt	-0.088	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.026	-0.046	0.000	0.021	0.009
Twist	0.000	-0.129	-0.145	0.000	0.009	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.005	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	1.514	1.864	0.000	-0.003	-0.071
Rise	0.000	1.864	7.500	0.000	-0.033	-0.190
Tilt	-0.067	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.003	-0.033	0.000	0.018	0.006
Twist	0.000	-0.071	-0.190	0.000	0.006	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.142	0.000	0.000	-0.097	0.000	0.000
Slide	0.000	1.665	1.919	0.000	0.001	-0.090
Rise	0.000	1.919	7.902	0.000	-0.057	-0.179
Tilt	-0.097	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.001	-0.057	0.000	0.021	0.005
Twist	0.000	-0.090	-0.179	0.000	0.005	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.764	0.000	0.000	-0.030	0.000	0.000
Slide	0.000	3.683	3.133	0.000	0.019	-0.068
Rise	0.000	3.133	10.660	0.000	0.083	-0.211
Tilt	-0.030	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.019	0.083	0.000	0.027	0.011
Twist	0.000	-0.068	-0.211	0.000	0.011	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.591	0.000	0.000	-0.034	0.000	0.000
Slide	0.000	2.341	1.287	0.000	0.045	-0.126
Rise	0.000	1.287	7.145	0.000	-0.056	-0.208
Tilt	-0.034	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.045	-0.056	0.000	0.016	0.008
Twist	0.000	-0.126	-0.208	0.000	0.008	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.435	0.000	0.000	-0.046	0.000	0.000
Slide	0.000	2.743	0.715	0.000	0.026	-0.146
Rise	0.000	0.716	8.452	0.000	-0.137	-0.247
Tilt	-0.046	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.026	-0.137	0.000	0.021	0.014
Twist	0.000	-0.146	-0.247	0.000	0.014	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.109	0.000	0.000	-0.112	0.000	0.000
Slide	0.000	2.158	1.680	0.000	-0.038	-0.112
Rise	0.000	1.680	9.253	0.000	-0.039	-0.173
Tilt	-0.112	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.038	-0.039	0.000	0.022	0.010
Twist	0.000	-0.112	-0.173	0.000	0.010	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.738	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	2.432	1.386	0.000	0.024	-0.095
Rise	0.000	1.386	6.849	0.000	-0.067	-0.212
Tilt	-0.090	0.000	0.000	0.031	0.000	0.000
Roll	0.000	0.024	-0.067	0.000	0.016	0.006
Twist	0.000	-0.095	-0.212	0.000	0.006	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.803	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	2.855	1.384	0.000	-0.053	-0.171
Rise	0.000	1.384	8.714	0.000	-0.017	-0.143
Tilt	-0.090	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.053	-0.017	0.000	0.022	0.006
Twist	0.000	-0.171	-0.143	0.000	0.006	0.036

[Image courtesy of Curves+]

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	Mean	Stdev
-acgt.2.cte	0.000	0.000

	Mean	Stdev
-acgt.21.cte	0.000	0.000

	Mean	Stdev
-agct.18.cte	0.000	0.000

	Mean	Stdev
-agct.4.cte	0.000	0.000

	Mean	Stdev
-atat.13.cte	0.000	0.000

	Mean	Stdev
-atat.15.cte	0.000	0.000

	Mean	Stdev
-atat.7.cte	0.000	0.000

	Mean	Stdev
-catg.12.cte	0.000	0.000

	Mean	Stdev
-catg.3.cte	0.000	0.000

	Mean	Stdev
-ccgg.11.cte	0.000	0.000

	Mean	Stdev
-gatc.20.cte	0.000	0.000

	Mean	Stdev
-gatc.6.cte	0.000	0.000

	Mean	Stdev
-ggcc.19.cte	0.000	0.000

	Mean	Stdev
-ggcc.5.cte	0.000	0.000

	Mean	Stdev
-gtac.9.cte	0.000	0.000

	Mean	Stdev
-tata.14.cte	0.000	0.000

	Mean	Stdev
-tata.17.cte	0.000	0.000

	Mean	Stdev
-tcga.10.cte	0.000	0.000

	Mean	Stdev
-tgca.8.cte	0.000	0.000

	Mean	Stdev
-ttaa.16.cte	0.000	0.000

	Mean	Stdev
rise-10-TCGA	3.347	0.276

	Mean	Stdev
roll-10-TCGA	1.151	5.533

	Mean	Stdev
shift-10-TCGA	0.000	0.885

	Mean	Stdev
slide-10-TCGA	-0.090	0.597

	Mean	Stdev
tilt-10-TCGA	0.000	5.012

	Mean	Stdev
twist-10-TCGA	38.540	4.845

	Mean	Stdev
rise-11-CCGG	3.361	0.308

	Mean	Stdev
roll-11-CCGG	5.095	6.090

	Mean	Stdev
shift-11-CCGG	0.000	1.021

	Mean	Stdev
slide-11-CCGG	-0.187	0.647

	Mean	Stdev
tilt-11-CCGG	0.000	4.683

	Mean	Stdev
twist-11-CCGG	32.193	6.071

	Mean	Stdev
rise-12-CATG	3.210	0.313

	Mean	Stdev
roll-12-CATG	4.811	6.526

	Mean	Stdev
shift-12-CATG	0.000	1.135

	Mean	Stdev
slide-12-CATG	0.135	0.571

	Mean	Stdev
tilt-12-CATG	0.000	5.471

	Mean	Stdev
twist-12-CATG	37.295	6.106

	Mean	Stdev
rise-13-ATAT	3.143	0.263

	Mean	Stdev
roll-13-ATAT	0.440	5.285

	Mean	Stdev
shift-13-ATAT	0.000	0.672

	Mean	Stdev
slide-13-ATAT	-0.700	0.408

	Mean	Stdev
tilt-13-ATAT	0.000	3.714

	Mean	Stdev
twist-13-ATAT	27.975	4.874

	Mean	Stdev
rise-14-TATA	3.491	0.323

	Mean	Stdev
roll-14-TATA	3.038	7.491

	Mean	Stdev
shift-14-TATA	0.000	1.035

	Mean	Stdev
slide-14-TATA	-0.121	0.653

	Mean	Stdev
tilt-14-TATA	0.000	4.875

	Mean	Stdev
twist-14-TATA	40.774	5.943

	Mean	Stdev
rise-15-ATAT	3.324	0.257

	Mean	Stdev
roll-15-ATAT	-2.411	4.389

	Mean	Stdev
shift-15-ATAT	0.000	0.578

	Mean	Stdev
slide-15-ATAT	-0.901	0.391

	Mean	Stdev
tilt-15-ATAT	0.000	3.621

	Mean	Stdev
twist-15-ATAT	31.790	3.469

	Mean	Stdev
rise-16-TTAA	3.301	0.273

	Mean	Stdev
roll-16-TTAA	0.134	5.305

	Mean	Stdev
shift-16-TTAA	0.000	1.031

	Mean	Stdev
slide-16-TTAA	-0.112	0.548

	Mean	Stdev
tilt-16-TTAA	0.000	5.137

	Mean	Stdev
twist-16-TTAA	37.018	4.867

	Mean	Stdev
rise-17-TATA	3.124	0.303

	Mean	Stdev
roll-17-TATA	7.143	7.792

	Mean	Stdev
shift-17-TATA	0.000	1.300

	Mean	Stdev
slide-17-TATA	0.056	0.645

	Mean	Stdev
tilt-17-TATA	0.000	5.571

	Mean	Stdev
twist-17-TATA	31.789	7.775

	Mean	Stdev
rise-18-AGCT	3.205	0.282

	Mean	Stdev
roll-18-AGCT	1.287	5.726

	Mean	Stdev
shift-18-AGCT	0.000	0.910

	Mean	Stdev
slide-18-AGCT	-0.454	0.665

	Mean	Stdev
tilt-18-AGCT	0.000	4.722

	Mean	Stdev
twist-18-AGCT	31.501	6.181

	Mean	Stdev
rise-19-GGCC	3.573	0.351

	Mean	Stdev
roll-19-GGCC	4.219	5.870

	Mean	Stdev
shift-19-GGCC	0.000	0.842

	Mean	Stdev
slide-19-GGCC	-0.689	0.759

	Mean	Stdev
tilt-19-GGCC	0.000	5.291

	Mean	Stdev
twist-19-GGCC	34.907	5.495

	Mean	Stdev
rise-2-ACGT	3.256	0.282

	Mean	Stdev
roll-2-ACGT	-1.239	5.782

	Mean	Stdev
shift-2-ACGT	0.000	0.961

	Mean	Stdev
slide-2-ACGT	-0.378	0.425

	Mean	Stdev
tilt-2-ACGT	0.000	3.863

	Mean	Stdev
twist-2-ACGT	30.939	4.884

	Mean	Stdev
rise-20-GATC	3.272	0.290

	Mean	Stdev
roll-20-GATC	0.669	5.577

	Mean	Stdev
shift-20-GATC	0.000	0.851

	Mean	Stdev
slide-20-GATC	-0.159	0.622

	Mean	Stdev
tilt-20-GATC	0.000	5.106

	Mean	Stdev
twist-20-GATC	37.167	5.629

	Mean	Stdev
rise-21-ACGT	3.198	0.303

	Mean	Stdev
roll-21-ACGT	0.120	5.213

	Mean	Stdev
shift-21-ACGT	0.000	0.802

	Mean	Stdev
slide-21-ACGT	-0.414	0.440

	Mean	Stdev
tilt-21-ACGT	0.000	4.455

	Mean	Stdev
twist-21-ACGT	28.464	5.656

	Mean	Stdev
rise-3-CATG	3.146	0.314

	Mean	Stdev
roll-3-CATG	5.302	7.084

	Mean	Stdev
shift-3-CATG	0.000	1.127

	Mean	Stdev
slide-3-CATG	0.120	0.586

	Mean	Stdev
tilt-3-CATG	0.000	5.321

	Mean	Stdev
twist-3-CATG	34.552	7.760

	Mean	Stdev
rise-4-AGCT	3.228	0.284

	Mean	Stdev
roll-4-AGCT	2.543	5.362

	Mean	Stdev
shift-4-AGCT	0.000	0.886

	Mean	Stdev
slide-4-AGCT	-0.312	0.603

	Mean	Stdev
tilt-4-AGCT	0.000	4.913

	Mean	Stdev
twist-4-AGCT	33.238	5.795

	Mean	Stdev
rise-5-GGCC	3.579	0.330

	Mean	Stdev
roll-5-GGCC	4.939	5.562

	Mean	Stdev
shift-5-GGCC	0.000	0.832

	Mean	Stdev
slide-5-GGCC	-0.672	0.736

	Mean	Stdev
tilt-5-GGCC	0.000	5.001

	Mean	Stdev
twist-5-GGCC	34.254	5.243

	Mean	Stdev
rise-6-GATC	3.399	0.287

	Mean	Stdev
roll-6-GATC	0.096	5.610

	Mean	Stdev
shift-6-GATC	0.000	0.757

	Mean	Stdev
slide-6-GATC	-0.301	0.615

	Mean	Stdev
tilt-6-GATC	0.000	4.773

	Mean	Stdev
twist-6-GATC	39.167	4.658

	Mean	Stdev
rise-7-ATAT	3.206	0.260

	Mean	Stdev
roll-7-ATAT	-1.538	4.989

	Mean	Stdev
shift-7-ATAT	0.000	0.554

	Mean	Stdev
slide-7-ATAT	-0.784	0.387

	Mean	Stdev
tilt-7-ATAT	0.000	3.497

	Mean	Stdev
twist-7-ATAT	29.370	4.377

	Mean	Stdev
rise-8-TGCA	3.416	0.350

	Mean	Stdev
roll-8-TGCA	6.567	6.645

	Mean	Stdev
shift-8-TGCA	0.000	1.110

	Mean	Stdev
slide-8-TGCA	-0.110	0.565

	Mean	Stdev
tilt-8-TGCA	0.000	5.397

	Mean	Stdev
twist-8-TGCA	38.173	6.257

	Mean	Stdev
rise-9-GTAC	3.287	0.294

	Mean	Stdev
roll-9-GTAC	-0.089	5.241

	Mean	Stdev
shift-9-GTAC	0.000	0.888

	Mean	Stdev
slide-9-GTAC	-0.605	0.461

	Mean	Stdev
tilt-9-GTAC	0.000	3.768

	Mean	Stdev
twist-9-GTAC	31.000	4.613

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20		21		22		23
5'	C	-	A	-	C	-	A	-	G	-	G	-	A	-	T	-	G	-	T	-	C	-	C	-	A	-	T	-	A	-	T	-	T	-	A	-	G	-	G	-	A	-	C	-	A	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	T	-	G	-	T	-	C	-	C	-	T	-	A	-	C	-	A	-	G	-	G	-	T	-	A	-	T	-	A	-	A	-	T	-	C	-	C	-	T	-	G	-	T	5'
	46		45		44		43		42		41		40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20		21		22		23
5'	C	-	A	-	C	-	A	-	G	-	G	-	A	-	T	-	G	-	T	-	C	-	C	-	A	-	T	-	A	-	T	-	T	-	A	-	G	-	G	-	A	-	C	-	A	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	T	-	G	-	T	-	C	-	C	-	T	-	A	-	C	-	A	-	G	-	G	-	T	-	A	-	T	-	A	-	A	-	T	-	C	-	C	-	T	-	G	-	T	5'
	46		45		44		43		42		41		40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20		21		22		23
5'	C	-	A	-	C	-	A	-	G	-	G	-	A	-	T	-	G	-	T	-	C	-	C	-	A	-	T	-	A	-	T	-	T	-	A	-	G	-	G	-	A	-	C	-	A	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	T	-	G	-	T	-	C	-	C	-	T	-	A	-	C	-	A	-	G	-	G	-	T	-	A	-	T	-	A	-	A	-	T	-	C	-	C	-	T	-	G	-	T	5'
	46		45		44		43		42		41		40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24