NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12		13		14
5'	T	-	G	-	C	-	C	-	C	-	T	-	C	-	T	-	T	-	A	-	T	-	A	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	A	-	C	-	G	-	G	-	G	-	A	-	G	-	A	-	A	-	T	-	A	-	T	-	C	-	G	5'
	28		27		26		25		24		23		22		21		20		19		18		17		16		15

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.499	0.677	0.298	-0.054	0.030	0.061
Slide	0.677	2.511	1.710	0.003	-0.028	-0.093
Rise	0.298	1.710	8.599	-0.177	-0.046	-0.224
Tilt	-0.054	0.003	-0.177	0.044	0.003	0.010
Roll	0.030	-0.028	-0.046	0.003	0.020	0.007
Twist	0.061	-0.093	-0.224	0.010	0.007	0.039

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.103	-0.061	-0.043	0.020	-0.004	-0.005
Slide	-0.061	4.770	2.751	0.008	-0.026	-0.057
Rise	-0.043	2.751	11.756	0.010	0.083	-0.128
Tilt	0.020	0.008	0.010	0.043	-0.001	0.001
Roll	-0.004	-0.026	0.083	-0.001	0.024	0.007
Twist	-0.005	-0.057	-0.128	0.001	0.007	0.030

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.389	0.038	-0.565	-0.128	-0.047	-0.037
Slide	0.038	1.509	1.825	0.003	-0.004	-0.077
Rise	-0.565	1.825	8.335	0.228	-0.029	-0.217
Tilt	-0.128	0.003	0.228	0.047	0.001	-0.002
Roll	-0.047	-0.004	-0.029	0.001	0.021	0.007
Twist	-0.037	-0.077	-0.217	-0.002	0.007	0.028

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.471	-0.096	-0.800	-0.146	-0.049	-0.052
Slide	-0.096	1.515	1.766	0.004	0.007	-0.108
Rise	-0.800	1.766	8.799	0.278	0.011	-0.174
Tilt	-0.146	0.004	0.278	0.047	0.003	-0.001
Roll	-0.049	0.007	0.011	0.003	0.021	0.006
Twist	-0.052	-0.108	-0.174	-0.001	0.006	0.035

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.305	-0.108	-0.124	-0.043	-0.040	-0.048
Slide	-0.108	1.955	1.717	0.005	-0.070	-0.095
Rise	-0.124	1.717	8.575	0.202	-0.040	-0.225
Tilt	-0.043	0.005	0.202	0.043	-0.001	-0.007
Roll	-0.040	-0.069	-0.040	-0.001	0.023	0.008
Twist	-0.048	-0.095	-0.225	-0.007	0.008	0.028

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.240	0.179	-0.283	-0.063	-0.061	-0.092
Slide	0.179	2.061	1.810	0.016	-0.052	-0.119
Rise	-0.283	1.810	8.067	0.220	-0.064	-0.224
Tilt	-0.063	0.016	0.220	0.044	-0.004	-0.010
Roll	-0.061	-0.052	-0.064	-0.004	0.022	0.010
Twist	-0.092	-0.119	-0.224	-0.010	0.010	0.037

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.230	0.214	0.559	-0.062	0.024	0.036
Slide	0.214	2.116	1.357	-0.081	-0.007	-0.048
Rise	0.559	1.357	7.176	-0.212	0.050	-0.150
Tilt	-0.062	-0.081	-0.212	0.030	-0.006	-0.002
Roll	0.024	-0.007	0.050	-0.006	0.020	0.002
Twist	0.036	-0.048	-0.150	-0.002	0.002	0.026

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.501	-0.119	0.165	-0.055	0.003	0.014
Slide	-0.119	2.192	0.846	-0.005	0.009	-0.134
Rise	0.165	0.846	7.766	-0.027	-0.079	-0.214
Tilt	-0.055	-0.005	-0.027	0.026	0.000	-0.000
Roll	0.003	0.009	-0.079	0.000	0.016	0.012
Twist	0.014	-0.134	-0.214	-0.000	0.012	0.032

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.499	0.186	-0.164	-0.049	-0.007	-0.005
Slide	0.186	2.078	1.215	0.004	0.011	-0.122
Rise	-0.164	1.215	7.555	0.020	-0.091	-0.210
Tilt	-0.049	0.004	0.020	0.024	0.001	0.001
Roll	-0.007	0.011	-0.091	0.001	0.015	0.010
Twist	-0.005	-0.122	-0.210	0.001	0.010	0.030

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.234	-0.517	-0.877	-0.097	-0.033	0.014
Slide	-0.517	2.089	1.267	0.017	-0.013	-0.102
Rise	-0.877	1.268	9.957	0.275	0.005	-0.179
Tilt	-0.097	0.017	0.275	0.043	0.005	-0.003
Roll	-0.033	-0.013	0.005	0.005	0.019	0.006
Twist	0.014	-0.102	-0.179	-0.003	0.006	0.025

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.393	-0.769	-0.514	-0.042	0.003	-0.045
Slide	-0.769	2.697	0.836	0.016	-0.074	-0.106
Rise	-0.514	0.836	9.605	0.234	-0.062	-0.201
Tilt	-0.042	0.016	0.234	0.044	-0.001	-0.009
Roll	0.003	-0.074	-0.062	-0.001	0.023	0.011
Twist	-0.045	-0.106	-0.201	-0.009	0.011	0.038

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-agct.12.cte	0.000	0.000

	Mean	Stdev
-atat.10.cte	0.000	0.000

	Mean	Stdev
-ccgg.3.cte	0.000	0.000

	Mean	Stdev
-ccgg.4.cte	0.000	0.000

	Mean	Stdev
-ctag.5.cte	0.000	0.000

	Mean	Stdev
-ctag.7.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-tata.11.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
-tcga.6.cte	0.000	0.000

	Mean	Stdev
-ttaa.8.cte	0.000	0.000

	Mean	Stdev
rise-10-ATAT	3.155	0.253

	Mean	Stdev
roll-10-ATAT	-1.629	5.357

	Mean	Stdev
shift-10-ATAT	-0.152	0.733

	Mean	Stdev
slide-10-ATAT	-0.685	0.382

	Mean	Stdev
tilt-10-ATAT	0.095	3.735

	Mean	Stdev
twist-10-ATAT	29.044	4.805

	Mean	Stdev
rise-11-TATA	3.200	0.307

	Mean	Stdev
roll-11-TATA	5.193	7.758

	Mean	Stdev
shift-11-TATA	0.403	1.259

	Mean	Stdev
slide-11-TATA	0.130	0.660

	Mean	Stdev
tilt-11-TATA	1.005	5.432

	Mean	Stdev
twist-11-TATA	35.209	6.617

	Mean	Stdev
rise-12-AGCT	3.365	0.305

	Mean	Stdev
roll-12-AGCT	2.506	5.706

	Mean	Stdev
shift-12-AGCT	-0.698	0.799

	Mean	Stdev
slide-12-AGCT	-0.344	0.622

	Mean	Stdev
tilt-12-AGCT	-3.414	4.211

	Mean	Stdev
twist-12-AGCT	35.051	4.909

	Mean	Stdev
rise-2-GCGC	3.387	0.365

	Mean	Stdev
roll-2-GCGC	-0.319	5.641

	Mean	Stdev
shift-2-GCGC	-0.047	0.754

	Mean	Stdev
slide-2-GCGC	-0.387	0.586

	Mean	Stdev
tilt-2-GCGC	0.570	5.469

	Mean	Stdev
twist-2-GCGC	34.350	5.579

	Mean	Stdev
rise-3-CCGG	3.462	0.364

	Mean	Stdev
roll-3-CCGG	2.481	5.716

	Mean	Stdev
shift-3-CCGG	-0.003	0.804

	Mean	Stdev
slide-3-CCGG	-0.793	0.757

	Mean	Stdev
tilt-3-CCGG	-0.065	4.435

	Mean	Stdev
twist-3-CCGG	33.999	5.584

	Mean	Stdev
rise-4-CCGG	3.543	0.340

	Mean	Stdev
roll-4-CCGG	2.410	5.612

	Mean	Stdev
shift-4-CCGG	0.038	0.827

	Mean	Stdev
slide-4-CCGG	-0.732	0.811

	Mean	Stdev
tilt-4-CCGG	-1.211	4.759

	Mean	Stdev
twist-4-CCGG	35.211	5.053

	Mean	Stdev
rise-5-CTAG	3.246	0.327

	Mean	Stdev
roll-5-CTAG	1.958	5.727

	Mean	Stdev
shift-5-CTAG	0.033	0.752

	Mean	Stdev
slide-5-CTAG	-0.665	0.678

	Mean	Stdev
tilt-5-CTAG	1.623	4.125

	Mean	Stdev
twist-5-CTAG	30.719	5.842

	Mean	Stdev
rise-6-TCGA	3.322	0.290

	Mean	Stdev
roll-6-TCGA	-0.139	6.038

	Mean	Stdev
shift-6-TCGA	0.148	0.822

	Mean	Stdev
slide-6-TCGA	-0.268	0.636

	Mean	Stdev
tilt-6-TCGA	0.982	4.426

	Mean	Stdev
twist-6-TCGA	36.279	5.933

	Mean	Stdev
rise-7-CTAG	3.460	0.339

	Mean	Stdev
roll-7-CTAG	3.421	5.896

	Mean	Stdev
shift-7-CTAG	0.166	0.920

	Mean	Stdev
slide-7-CTAG	-0.626	0.643

	Mean	Stdev
tilt-7-CTAG	1.691	4.438

	Mean	Stdev
twist-7-CTAG	34.369	5.537

	Mean	Stdev
rise-8-TTAA	3.241	0.280

	Mean	Stdev
roll-8-TTAA	2.251	5.651

	Mean	Stdev
shift-8-TTAA	0.519	0.803

	Mean	Stdev
slide-8-TTAA	-0.024	0.586

	Mean	Stdev
tilt-8-TTAA	3.596	4.054

	Mean	Stdev
twist-8-TTAA	34.458	5.146

	Mean	Stdev
rise-9-TATA	3.271	0.320

	Mean	Stdev
roll-9-TATA	4.381	7.798

	Mean	Stdev
shift-9-TATA	-0.395	1.278

	Mean	Stdev
slide-9-TATA	0.210	0.697

	Mean	Stdev
tilt-9-TATA	-3.206	5.550

	Mean	Stdev
twist-9-TATA	36.590	6.453

	1		2		3		4		5		6		7		8		9		10		11		12		13		14
5'	T	-	G	-	C	-	C	-	C	-	T	-	C	-	T	-	T	-	A	-	T	-	A	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	A	-	C	-	G	-	G	-	G	-	A	-	G	-	A	-	A	-	T	-	A	-	T	-	C	-	G	5'
	28		27		26		25		24		23		22		21		20		19		18		17		16		15

	1		2		3		4		5		6		7		8		9		10		11		12		13		14
5'	T	-	G	-	C	-	C	-	C	-	T	-	C	-	T	-	T	-	A	-	T	-	A	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	A	-	C	-	G	-	G	-	G	-	A	-	G	-	A	-	A	-	T	-	A	-	T	-	C	-	G	5'
	28		27		26		25		24		23		22		21		20		19		18		17		16		15

	1		2		3		4		5		6		7		8		9		10		11		12		13		14
5'	T	-	G	-	C	-	C	-	C	-	T	-	C	-	T	-	T	-	A	-	T	-	A	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	A	-	C	-	G	-	G	-	G	-	A	-	G	-	A	-	A	-	T	-	A	-	T	-	C	-	G	5'
	28		27		26		25		24		23		22		21		20		19		18		17		16		15