NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.869	0.000	0.000	-0.072	0.000	0.000
Slide	0.000	2.466	0.754	0.000	-0.045	-0.149
Rise	0.000	0.754	9.323	0.000	-0.009	-0.161
Tilt	-0.072	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.045	-0.009	0.000	0.020	0.007
Twist	0.000	-0.149	-0.161	0.000	0.007	0.030

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.568	0.000	0.000	-0.072	0.000	0.000
Slide	0.000	2.882	1.144	0.000	-0.073	-0.153
Rise	0.000	1.144	8.161	0.000	-0.073	-0.185
Tilt	-0.072	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.073	-0.073	0.000	0.024	0.009
Twist	0.000	-0.153	-0.185	0.000	0.009	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.566	0.000	0.000	-0.074	0.000	0.000
Slide	0.000	2.992	1.271	0.000	-0.083	-0.158
Rise	0.000	1.271	8.897	0.000	-0.062	-0.201
Tilt	-0.074	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.083	-0.062	0.000	0.024	0.009
Twist	0.000	-0.158	-0.201	0.000	0.009	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.831	0.000	0.000	-0.044	0.000	0.000
Slide	0.000	3.419	2.816	0.000	0.031	-0.056
Rise	0.000	2.816	10.500	0.000	0.080	-0.176
Tilt	-0.044	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.031	0.080	0.000	0.025	0.009
Twist	0.000	-0.056	-0.176	0.000	0.009	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.863	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	3.703	2.982	0.000	0.029	-0.113
Rise	0.000	2.982	10.821	0.000	0.091	-0.211
Tilt	-0.037	0.000	0.000	0.045	0.000	0.000
Roll	0.000	0.029	0.091	0.000	0.028	0.010
Twist	0.000	-0.113	-0.211	0.000	0.010	0.043

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.144	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	2.258	1.839	0.000	-0.065	-0.059
Rise	0.000	1.839	7.821	0.000	-0.062	-0.181
Tilt	-0.061	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.065	-0.062	0.000	0.022	0.005
Twist	0.000	-0.059	-0.181	0.000	0.005	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.816	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	2.339	1.572	0.000	-0.059	-0.111
Rise	0.000	1.572	8.739	0.000	-0.049	-0.196
Tilt	-0.067	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.059	-0.049	0.000	0.020	0.006
Twist	0.000	-0.111	-0.196	0.000	0.006	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.694	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	5.177	3.220	0.000	-0.086	-0.055
Rise	0.000	3.220	11.264	0.000	0.050	-0.164
Tilt	-0.029	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.086	0.050	0.000	0.031	0.012
Twist	0.000	-0.055	-0.164	0.000	0.012	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.685	0.000	0.000	-0.062	0.000	0.000
Slide	0.000	2.481	0.738	0.000	0.019	-0.103
Rise	0.000	0.738	7.618	0.000	-0.135	-0.239
Tilt	-0.062	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.019	-0.134	0.000	0.019	0.011
Twist	0.000	-0.103	-0.239	0.000	0.011	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.648	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.593	1.063	0.000	0.023	-0.112
Rise	0.000	1.063	7.215	0.000	-0.082	-0.209
Tilt	-0.077	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.023	-0.082	0.000	0.016	0.007
Twist	0.000	-0.112	-0.209	0.000	0.007	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.667	0.000	0.000	-0.070	0.000	0.000
Slide	0.000	2.819	0.753	0.000	0.017	-0.126
Rise	0.000	0.753	7.411	0.000	-0.089	-0.219
Tilt	-0.070	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.017	-0.089	0.000	0.016	0.008
Twist	0.000	-0.126	-0.219	0.000	0.008	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.712	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	2.315	1.408	0.000	0.020	-0.086
Rise	0.000	1.408	6.584	0.000	-0.069	-0.171
Tilt	-0.081	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.020	-0.069	0.000	0.016	0.005
Twist	0.000	-0.086	-0.171	0.000	0.005	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.038	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	1.974	1.275	0.000	-0.026	-0.128
Rise	0.000	1.275	7.063	0.000	-0.047	-0.151
Tilt	-0.054	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.026	-0.047	0.000	0.021	0.007
Twist	0.000	-0.128	-0.151	0.000	0.007	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.027	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	2.100	1.482	0.000	-0.028	-0.120
Rise	0.000	1.482	7.822	0.000	-0.048	-0.167
Tilt	-0.061	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.028	-0.048	0.000	0.023	0.010
Twist	0.000	-0.120	-0.167	0.000	0.010	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.957	0.000	0.000	-0.115	0.000	0.000
Slide	0.000	2.225	1.560	0.000	-0.038	-0.095
Rise	0.000	1.560	8.628	0.000	-0.024	-0.149
Tilt	-0.115	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.038	-0.024	0.000	0.021	0.011
Twist	0.000	-0.095	-0.149	0.000	0.011	0.036

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.11.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-acgt.15.cte	0.000	0.000

	Mean	Stdev
-acgt.6.cte	0.000	0.000

	Mean	Stdev
-agct.12.cte	0.000	0.000

	Mean	Stdev
-agct.3.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-catg.10.cte	0.000	0.000

	Mean	Stdev
-catg.16.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-catg.7.cte	0.000	0.000

	Mean	Stdev
-gatc.13.cte	0.000	0.000

	Mean	Stdev
-gatc.4.cte	0.000	0.000

	Mean	Stdev
-tcga.9.cte	0.000	0.000

	Mean	Stdev
rise-10-CATG	3.156	0.334

	Mean	Stdev
roll-10-CATG	8.539	6.893

	Mean	Stdev
shift-10-CATG	0.000	1.032

	Mean	Stdev
slide-10-CATG	-0.003	0.576

	Mean	Stdev
tilt-10-CATG	0.000	5.088

	Mean	Stdev
twist-10-CATG	30.002	7.270

	Mean	Stdev
rise-11-AATT	3.195	0.264

	Mean	Stdev
roll-11-AATT	-0.011	5.694

	Mean	Stdev
shift-11-AATT	0.000	0.934

	Mean	Stdev
slide-11-AATT	-0.081	0.582

	Mean	Stdev
tilt-11-AATT	0.000	5.377

	Mean	Stdev
twist-11-AATT	35.311	5.566

	Mean	Stdev
rise-12-AGCT	3.335	0.328

	Mean	Stdev
roll-12-AGCT	4.140	5.488

	Mean	Stdev
shift-12-AGCT	0.000	0.753

	Mean	Stdev
slide-12-AGCT	-0.457	0.587

	Mean	Stdev
tilt-12-AGCT	0.000	4.372

	Mean	Stdev
twist-12-AGCT	32.256	5.849

	Mean	Stdev
rise-13-GATC	3.486	0.312

	Mean	Stdev
roll-13-GATC	1.453	5.467

	Mean	Stdev
shift-13-GATC	0.000	0.788

	Mean	Stdev
slide-13-GATC	-0.198	0.657

	Mean	Stdev
tilt-13-GATC	0.000	4.540

	Mean	Stdev
twist-13-GATC	37.757	5.506

	Mean	Stdev
rise-14-AATT	3.286	0.287

	Mean	Stdev
roll-14-AATT	0.163	5.206

	Mean	Stdev
shift-14-AATT	0.000	0.652

	Mean	Stdev
slide-14-AATT	-0.488	0.522

	Mean	Stdev
tilt-14-AATT	0.000	4.906

	Mean	Stdev
twist-14-AATT	35.884	4.919

	Mean	Stdev
rise-15-ACGT	3.350	0.284

	Mean	Stdev
roll-15-ACGT	-1.240	5.295

	Mean	Stdev
shift-15-ACGT	0.000	0.865

	Mean	Stdev
slide-15-ACGT	-0.618	0.470

	Mean	Stdev
tilt-15-ACGT	0.000	3.907

	Mean	Stdev
twist-15-ACGT	32.344	4.765

	Mean	Stdev
rise-16-CATG	3.253	0.334

	Mean	Stdev
roll-16-CATG	5.115	6.794

	Mean	Stdev
shift-16-CATG	0.000	1.153

	Mean	Stdev
slide-16-CATG	0.082	0.565

	Mean	Stdev
tilt-16-CATG	0.000	5.493

	Mean	Stdev
twist-16-CATG	36.092	6.914

	Mean	Stdev
rise-2-CATG	3.124	0.333

	Mean	Stdev
roll-2-CATG	5.786	6.982

	Mean	Stdev
shift-2-CATG	0.000	1.116

	Mean	Stdev
slide-2-CATG	0.098	0.562

	Mean	Stdev
tilt-2-CATG	0.000	5.814

	Mean	Stdev
twist-2-CATG	33.265	7.479

	Mean	Stdev
rise-3-AGCT	3.208	0.296

	Mean	Stdev
roll-3-AGCT	2.326	5.796

	Mean	Stdev
shift-3-AGCT	0.000	0.924

	Mean	Stdev
slide-3-AGCT	-0.266	0.594

	Mean	Stdev
tilt-3-AGCT	0.000	4.486

	Mean	Stdev
twist-3-AGCT	31.783	6.739

	Mean	Stdev
rise-4-GATC	3.473	0.300

	Mean	Stdev
roll-4-GATC	1.119	5.483

	Mean	Stdev
shift-4-GATC	0.000	0.803

	Mean	Stdev
slide-4-GATC	-0.158	0.620

	Mean	Stdev
tilt-4-GATC	0.000	4.581

	Mean	Stdev
twist-4-GATC	37.987	5.410

	Mean	Stdev
rise-5-AATT	3.292	0.276

	Mean	Stdev
roll-5-AATT	0.271	5.291

	Mean	Stdev
shift-5-AATT	0.000	0.651

	Mean	Stdev
slide-5-AATT	-0.440	0.517

	Mean	Stdev
tilt-5-AATT	0.000	4.729

	Mean	Stdev
twist-5-AATT	35.900	4.892

	Mean	Stdev
rise-6-ACGT	3.336	0.277

	Mean	Stdev
roll-6-ACGT	-1.104	4.990

	Mean	Stdev
shift-6-ACGT	0.000	0.839

	Mean	Stdev
slide-6-ACGT	-0.673	0.459

	Mean	Stdev
tilt-6-ACGT	0.000	3.695

	Mean	Stdev
twist-6-ACGT	32.309	4.244

	Mean	Stdev
rise-7-CATG	3.487	0.346

	Mean	Stdev
roll-7-CATG	7.166	6.599

	Mean	Stdev
shift-7-CATG	0.000	1.103

	Mean	Stdev
slide-7-CATG	-0.239	0.578

	Mean	Stdev
tilt-7-CATG	0.000	5.498

	Mean	Stdev
twist-7-CATG	38.144	5.859

	Mean	Stdev
rise-8-ATAT	3.240	0.269

	Mean	Stdev
roll-8-ATAT	-1.370	4.943

	Mean	Stdev
shift-8-ATAT	0.000	0.591

	Mean	Stdev
slide-8-ATAT	-0.806	0.388

	Mean	Stdev
tilt-8-ATAT	0.000	3.633

	Mean	Stdev
twist-8-ATAT	29.900	4.240

	Mean	Stdev
rise-9-TCGA	3.353	0.284

	Mean	Stdev
roll-9-TCGA	1.062	5.860

	Mean	Stdev
shift-9-TCGA	0.000	1.032

	Mean	Stdev
slide-9-TCGA	0.102	0.572

	Mean	Stdev
tilt-9-TCGA	0.000	5.581

	Mean	Stdev
twist-9-TCGA	38.220	4.717

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