NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.888	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	3.292	0.983	0.000	-0.062	-0.226
Rise	0.000	0.983	9.622	0.000	0.000	-0.186
Tilt	-0.090	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.062	0.000	0.000	0.023	0.011
Twist	0.000	-0.226	-0.186	0.000	0.011	0.040

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.877	0.000	0.000	-0.063	0.000	0.000
Slide	0.000	2.843	0.712	0.000	-0.071	-0.165
Rise	0.000	0.712	9.699	0.000	-0.005	-0.162
Tilt	-0.063	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.071	-0.005	0.000	0.024	0.010
Twist	0.000	-0.165	-0.162	0.000	0.010	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.732	0.000	0.000	-0.071	0.000	0.000
Slide	0.000	4.353	3.433	0.000	0.048	-0.170
Rise	0.000	3.433	10.889	0.000	0.087	-0.229
Tilt	-0.071	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.048	0.087	0.000	0.031	0.005
Twist	0.000	-0.170	-0.229	0.000	0.005	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.689	0.000	0.000	-0.040	0.000	0.000
Slide	0.000	4.516	3.264	0.000	0.021	-0.133
Rise	0.000	3.264	10.232	0.000	0.054	-0.225
Tilt	-0.040	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.021	0.054	0.000	0.029	0.006
Twist	0.000	-0.133	-0.225	0.000	0.006	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.486	0.000	0.000	-0.123	0.000	0.000
Slide	0.000	3.349	1.760	0.000	-0.054	-0.219
Rise	0.000	1.760	7.850	0.000	-0.055	-0.170
Tilt	-0.123	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.054	-0.055	0.000	0.025	0.004
Twist	0.000	-0.219	-0.170	0.000	0.004	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.881	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	2.838	0.715	0.000	0.006	-0.109
Rise	0.000	0.715	9.025	0.000	-0.153	-0.276
Tilt	-0.059	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.006	-0.153	0.000	0.022	0.012
Twist	0.000	-0.109	-0.276	0.000	0.012	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.738	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	2.516	1.749	0.000	-0.029	-0.125
Rise	0.000	1.749	9.007	0.000	-0.026	-0.188
Tilt	-0.106	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.029	-0.026	0.000	0.020	0.005
Twist	0.000	-0.125	-0.188	0.000	0.005	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.716	0.000	0.000	-0.034	0.000	0.000
Slide	0.000	4.714	3.429	0.000	0.019	-0.156
Rise	0.000	3.429	11.915	0.000	0.092	-0.211
Tilt	-0.034	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.019	0.092	0.000	0.030	0.004
Twist	0.000	-0.156	-0.211	0.000	0.004	0.049

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.407	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	2.670	0.695	0.000	0.004	-0.157
Rise	0.000	0.695	8.474	0.000	-0.112	-0.224
Tilt	-0.029	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.004	-0.112	0.000	0.019	0.014
Twist	0.000	-0.157	-0.224	0.000	0.014	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.888	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	2.850	1.355	0.000	-0.049	-0.132
Rise	0.000	1.355	9.447	0.000	-0.034	-0.167
Tilt	-0.106	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.049	-0.034	0.000	0.024	0.011
Twist	0.000	-0.132	-0.167	0.000	0.011	0.034

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-acgt.11.cte	0.000	0.000

	Mean	Stdev
-acgt.2.cte	0.000	0.000

	Mean	Stdev
-agct.6.cte	0.000	0.000

	Mean	Stdev
-catg.9.cte	0.000	0.000

	Mean	Stdev
-ctag.3.cte	0.000	0.000

	Mean	Stdev
-gtac.7.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tcga.8.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.254	0.261

	Mean	Stdev
roll-10-AATT	-1.059	5.480

	Mean	Stdev
shift-10-AATT	0.000	0.946

	Mean	Stdev
slide-10-AATT	-0.083	0.540

	Mean	Stdev
tilt-10-AATT	0.000	4.798

	Mean	Stdev
twist-10-AATT	36.808	5.340

	Mean	Stdev
rise-11-ACGT	3.456	0.274

	Mean	Stdev
roll-11-ACGT	-1.440	4.526

	Mean	Stdev
shift-11-ACGT	0.000	0.603

	Mean	Stdev
slide-11-ACGT	-0.765	0.442

	Mean	Stdev
tilt-11-ACGT	0.000	3.811

	Mean	Stdev
twist-11-ACGT	34.376	3.979

	Mean	Stdev
rise-2-ACGT	3.513	0.282

	Mean	Stdev
roll-2-ACGT	-1.673	4.655

	Mean	Stdev
shift-2-ACGT	0.000	0.597

	Mean	Stdev
slide-2-ACGT	-0.873	0.417

	Mean	Stdev
tilt-2-ACGT	0.000	4.085

	Mean	Stdev
twist-2-ACGT	33.898	3.943

	Mean	Stdev
rise-3-CTAG	3.361	0.280

	Mean	Stdev
roll-3-CTAG	1.297	5.500

	Mean	Stdev
shift-3-CTAG	0.000	1.188

	Mean	Stdev
slide-3-CTAG	-0.296	0.549

	Mean	Stdev
tilt-3-CTAG	0.000	5.480

	Mean	Stdev
twist-3-CTAG	37.091	4.398

	Mean	Stdev
rise-4-TATA	3.066	0.295

	Mean	Stdev
roll-4-TATA	7.597	7.662

	Mean	Stdev
shift-4-TATA	0.000	1.257

	Mean	Stdev
slide-4-TATA	-0.031	0.643

	Mean	Stdev
tilt-4-TATA	0.000	5.182

	Mean	Stdev
twist-4-TATA	29.749	7.701

	Mean	Stdev
rise-5-AATT	3.199	0.258

	Mean	Stdev
roll-5-AATT	0.105	5.356

	Mean	Stdev
shift-5-AATT	0.000	0.891

	Mean	Stdev
slide-5-AATT	-0.119	0.543

	Mean	Stdev
tilt-5-AATT	0.000	4.893

	Mean	Stdev
twist-5-AATT	34.631	5.464

	Mean	Stdev
rise-6-AGCT	3.528	0.294

	Mean	Stdev
roll-6-AGCT	2.707	4.954

	Mean	Stdev
shift-6-AGCT	0.000	0.745

	Mean	Stdev
slide-6-AGCT	-0.759	0.523

	Mean	Stdev
tilt-6-AGCT	0.000	4.839

	Mean	Stdev
twist-6-AGCT	36.833	4.317

	Mean	Stdev
rise-7-GTAC	3.445	0.256

	Mean	Stdev
roll-7-GTAC	-1.472	4.543

	Mean	Stdev
shift-7-GTAC	0.000	0.590

	Mean	Stdev
slide-7-GTAC	-0.891	0.408

	Mean	Stdev
tilt-7-GTAC	0.000	3.913

	Mean	Stdev
twist-7-GTAC	33.915	3.736

	Mean	Stdev
rise-8-TCGA	3.311	0.264

	Mean	Stdev
roll-8-TCGA	0.643	5.314

	Mean	Stdev
shift-8-TCGA	0.000	0.989

	Mean	Stdev
slide-8-TCGA	0.124	0.508

	Mean	Stdev
tilt-8-TCGA	0.000	4.743

	Mean	Stdev
twist-8-TCGA	38.171	4.982

	Mean	Stdev
rise-9-CATG	3.026	0.319

	Mean	Stdev
roll-9-CATG	9.028	6.463

	Mean	Stdev
shift-9-CATG	0.000	0.876

	Mean	Stdev
slide-9-CATG	0.021	0.531

	Mean	Stdev
tilt-9-CATG	0.000	4.796

	Mean	Stdev
twist-9-CATG	27.192	7.750

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