NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.738	0.000	0.000	-0.099	0.000	0.000
Slide	0.000	2.597	0.981	0.000	-0.071	-0.158
Rise	0.000	0.981	9.770	0.000	-0.039	-0.192
Tilt	-0.099	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.071	-0.039	0.000	0.022	0.010
Twist	0.000	-0.158	-0.192	0.000	0.010	0.039

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.097	0.000	0.000	-0.108	0.000	0.000
Slide	0.000	2.644	1.135	0.000	-0.057	-0.186
Rise	0.000	1.135	8.902	0.000	-0.031	-0.190
Tilt	-0.108	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.057	-0.031	0.000	0.024	0.008
Twist	0.000	-0.186	-0.190	0.000	0.008	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.796	0.000	0.000	-0.070	0.000	0.000
Slide	0.000	2.590	1.197	0.000	-0.078	-0.156
Rise	0.000	1.197	9.120	0.000	-0.028	-0.175
Tilt	-0.070	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.078	-0.028	0.000	0.024	0.011
Twist	0.000	-0.156	-0.175	0.000	0.011	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.738	0.000	0.000	-0.069	0.000	0.000
Slide	0.000	3.183	1.315	0.000	-0.061	-0.167
Rise	0.000	1.315	8.245	0.000	-0.051	-0.155
Tilt	-0.069	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.061	-0.051	0.000	0.025	0.008
Twist	0.000	-0.167	-0.155	0.000	0.008	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.043	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	4.003	1.236	0.000	-0.041	-0.307
Rise	0.000	1.236	8.016	0.000	-0.056	-0.152
Tilt	-0.095	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.041	-0.056	0.000	0.024	0.005
Twist	0.000	-0.307	-0.152	0.000	0.005	0.056

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.873	0.000	0.000	-0.049	0.000	0.000
Slide	0.000	3.682	3.072	0.000	0.023	-0.070
Rise	0.000	3.072	10.868	0.000	0.077	-0.198
Tilt	-0.049	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.023	0.077	0.000	0.026	0.009
Twist	0.000	-0.070	-0.198	0.000	0.009	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.752	0.000	0.000	-0.028	0.000	0.000
Slide	0.000	5.104	2.898	0.000	-0.024	-0.166
Rise	0.000	2.898	10.665	0.000	0.028	-0.186
Tilt	-0.028	0.000	0.000	0.051	0.000	0.000
Roll	0.000	-0.024	0.028	0.000	0.032	0.010
Twist	0.000	-0.166	-0.186	0.000	0.010	0.057

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.553	0.000	0.000	-0.007	0.000	0.000
Slide	0.000	4.969	3.036	0.000	-0.024	-0.173
Rise	0.000	3.036	11.662	0.000	0.069	-0.175
Tilt	-0.007	0.000	0.000	0.046	0.000	0.000
Roll	0.000	-0.024	0.069	0.000	0.031	0.011
Twist	0.000	-0.173	-0.175	0.000	0.011	0.060

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.787	0.000	0.000	-0.063	0.000	0.000
Slide	0.000	2.230	1.422	0.000	0.020	-0.090
Rise	0.000	1.422	7.000	0.000	-0.108	-0.238
Tilt	-0.063	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.020	-0.108	0.000	0.019	0.009
Twist	0.000	-0.090	-0.238	0.000	0.009	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.506	0.000	0.000	-0.099	0.000	0.000
Slide	0.000	2.625	1.734	0.000	-0.050	-0.120
Rise	0.000	1.734	7.499	0.000	-0.039	-0.138
Tilt	-0.099	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.050	-0.039	0.000	0.024	0.005
Twist	0.000	-0.120	-0.138	0.000	0.005	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.474	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	2.318	0.978	0.000	0.032	-0.113
Rise	0.000	0.978	7.035	0.000	-0.078	-0.209
Tilt	-0.053	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.032	-0.078	0.000	0.016	0.010
Twist	0.000	-0.113	-0.209	0.000	0.010	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.406	0.000	0.000	-0.044	0.000	0.000
Slide	0.000	2.443	0.955	0.000	-0.003	-0.149
Rise	0.000	0.955	8.475	0.000	-0.136	-0.234
Tilt	-0.044	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.003	-0.136	0.000	0.020	0.015
Twist	0.000	-0.149	-0.234	0.000	0.015	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.422	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	2.404	1.354	0.000	0.031	-0.113
Rise	0.000	1.354	7.971	0.000	-0.108	-0.245
Tilt	-0.061	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.031	-0.108	0.000	0.019	0.013
Twist	0.000	-0.113	-0.245	0.000	0.013	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.723	0.000	0.000	-0.065	0.000	0.000
Slide	0.000	2.754	0.702	0.000	-0.065	-0.149
Rise	0.000	0.702	9.581	0.000	-0.005	-0.183
Tilt	-0.065	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.065	-0.005	0.000	0.021	0.010
Twist	0.000	-0.149	-0.183	0.000	0.010	0.033

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.13.cte	0.000	0.000

	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-aatt.7.cte	0.000	0.000

	Mean	Stdev
-acgt.11.cte	0.000	0.000

	Mean	Stdev
-atat.14.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-catg.12.cte	0.000	0.000

	Mean	Stdev
-ctag.2.cte	0.000	0.000

	Mean	Stdev
-tata.15.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
-ttaa.3.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.217	0.258

	Mean	Stdev
roll-10-AATT	0.977	5.592

	Mean	Stdev
shift-10-AATT	0.000	1.168

	Mean	Stdev
slide-10-AATT	0.043	0.554

	Mean	Stdev
tilt-10-AATT	0.000	5.630

	Mean	Stdev
twist-10-AATT	36.009	4.706

	Mean	Stdev
rise-11-ACGT	3.356	0.282

	Mean	Stdev
roll-11-ACGT	-1.100	5.114

	Mean	Stdev
shift-11-ACGT	0.000	0.848

	Mean	Stdev
slide-11-ACGT	-0.668	0.457

	Mean	Stdev
tilt-11-ACGT	0.000	3.899

	Mean	Stdev
twist-11-ACGT	32.509	4.460

	Mean	Stdev
rise-12-CATG	3.364	0.370

	Mean	Stdev
roll-12-CATG	7.849	6.700

	Mean	Stdev
shift-12-CATG	0.000	0.963

	Mean	Stdev
slide-12-CATG	-0.194	0.609

	Mean	Stdev
tilt-12-CATG	0.000	5.316

	Mean	Stdev
twist-12-CATG	34.207	7.574

	Mean	Stdev
rise-13-AATT	3.335	0.270

	Mean	Stdev
roll-13-AATT	0.052	5.085

	Mean	Stdev
shift-13-AATT	0.000	0.901

	Mean	Stdev
slide-13-AATT	-0.229	0.561

	Mean	Stdev
tilt-13-AATT	0.000	5.345

	Mean	Stdev
twist-13-AATT	37.355	4.295

	Mean	Stdev
rise-14-ATAT	3.351	0.259

	Mean	Stdev
roll-14-ATAT	-2.475	4.451

	Mean	Stdev
shift-14-ATAT	0.000	0.581

	Mean	Stdev
slide-14-ATAT	-0.853	0.380

	Mean	Stdev
tilt-14-ATAT	0.000	3.421

	Mean	Stdev
twist-14-ATAT	32.414	3.480

	Mean	Stdev
rise-15-TATA	3.326	0.322

	Mean	Stdev
roll-15-TATA	2.850	7.266

	Mean	Stdev
shift-15-TATA	0.000	1.253

	Mean	Stdev
slide-15-TATA	0.158	0.604

	Mean	Stdev
tilt-15-TATA	0.000	5.211

	Mean	Stdev
twist-15-TATA	38.852	5.581

	Mean	Stdev
rise-2-CTAG	3.465	0.307

	Mean	Stdev
roll-2-CTAG	2.084	5.053

	Mean	Stdev
shift-2-CTAG	0.000	0.696

	Mean	Stdev
slide-2-CTAG	-0.730	0.551

	Mean	Stdev
tilt-2-CTAG	0.000	4.663

	Mean	Stdev
twist-2-CTAG	35.376	4.734

	Mean	Stdev
rise-3-TTAA	3.209	0.264

	Mean	Stdev
roll-3-TTAA	-0.246	5.821

	Mean	Stdev
shift-3-TTAA	0.000	1.004

	Mean	Stdev
slide-3-TTAA	-0.038	0.533

	Mean	Stdev
tilt-3-TTAA	0.000	4.969

	Mean	Stdev
twist-3-TTAA	35.127	5.395

	Mean	Stdev
rise-4-TATA	3.121	0.298

	Mean	Stdev
roll-4-TATA	7.243	7.658

	Mean	Stdev
shift-4-TATA	0.000	1.331

	Mean	Stdev
slide-4-TATA	0.181	0.667

	Mean	Stdev
tilt-4-TATA	0.000	5.263

	Mean	Stdev
twist-4-TATA	31.531	7.872

	Mean	Stdev
rise-5-AATT	3.251	0.269

	Mean	Stdev
roll-5-AATT	1.092	5.501

	Mean	Stdev
shift-5-AATT	0.000	0.947

	Mean	Stdev
slide-5-AATT	-0.066	0.569

	Mean	Stdev
tilt-5-AATT	0.000	4.578

	Mean	Stdev
twist-5-AATT	35.301	5.224

	Mean	Stdev
rise-6-AATT	3.382	0.282

	Mean	Stdev
roll-6-AATT	0.742	5.056

	Mean	Stdev
shift-6-AATT	0.000	0.603

	Mean	Stdev
slide-6-AATT	-0.481	0.509

	Mean	Stdev
tilt-6-AATT	0.000	4.137

	Mean	Stdev
twist-6-AATT	35.239	5.213

	Mean	Stdev
rise-7-AATT	3.378	0.280

	Mean	Stdev
roll-7-AATT	0.002	4.970

	Mean	Stdev
shift-7-AATT	0.000	0.582

	Mean	Stdev
slide-7-AATT	-0.559	0.506

	Mean	Stdev
tilt-7-AATT	0.000	4.888

	Mean	Stdev
twist-7-AATT	37.300	4.298

	Mean	Stdev
rise-8-ATAT	3.293	0.250

	Mean	Stdev
roll-8-ATAT	-2.596	4.586

	Mean	Stdev
shift-8-ATAT	0.000	0.615

	Mean	Stdev
slide-8-ATAT	-0.874	0.388

	Mean	Stdev
tilt-8-ATAT	0.000	3.568

	Mean	Stdev
twist-8-ATAT	32.329	3.431

	Mean	Stdev
rise-9-TATA	3.229	0.314

	Mean	Stdev
roll-9-TATA	4.732	7.405

	Mean	Stdev
shift-9-TATA	0.000	1.443

	Mean	Stdev
slide-9-TATA	0.121	0.616

	Mean	Stdev
tilt-9-TATA	0.000	5.808

	Mean	Stdev
twist-9-TATA	35.099	6.208

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