NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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3'	G	-	C	-	A	-	A	-	T	-	A	-	A	-	C	-	C	-	G	-	T	-	C	-	C	-	T	-	T	-	C	-	G	-	T	-	G	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.268	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	2.609	0.822	0.000	-0.088	-0.138
Rise	0.000	0.822	8.829	0.000	-0.059	-0.193
Tilt	-0.059	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.088	-0.059	0.000	0.024	0.011
Twist	0.000	-0.138	-0.193	0.000	0.011	0.031

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.840	0.000	0.000	-0.075	0.000	0.000
Slide	0.000	2.385	1.753	0.000	-0.058	-0.132
Rise	0.000	1.753	9.218	0.000	-0.017	-0.200
Tilt	-0.075	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.058	-0.017	0.000	0.021	0.006
Twist	0.000	-0.132	-0.200	0.000	0.006	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.558	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	2.814	1.424	0.000	-0.072	-0.128
Rise	0.000	1.424	8.126	0.000	-0.038	-0.148
Tilt	-0.106	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.072	-0.038	0.000	0.022	0.003
Twist	0.000	-0.128	-0.148	0.000	0.003	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.958	0.000	0.000	0.016	0.000	0.000
Slide	0.000	4.315	2.426	0.000	-0.045	-0.158
Rise	0.000	2.426	10.531	0.000	0.034	-0.185
Tilt	0.016	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.045	0.034	0.000	0.026	0.007
Twist	0.000	-0.158	-0.185	0.000	0.007	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.742	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	3.074	0.763	0.000	0.011	-0.120
Rise	0.000	0.763	7.989	0.000	-0.096	-0.212
Tilt	-0.077	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.011	-0.096	0.000	0.018	0.009
Twist	0.000	-0.120	-0.212	0.000	0.009	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.667	0.000	0.000	-0.078	0.000	0.000
Slide	0.000	2.974	0.578	0.000	0.027	-0.120
Rise	0.000	0.578	7.707	0.000	-0.091	-0.202
Tilt	-0.078	0.000	0.000	0.031	0.000	0.000
Roll	0.000	0.027	-0.091	0.000	0.019	0.010
Twist	0.000	-0.120	-0.202	0.000	0.010	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.009	0.000	0.000	-0.086	0.000	0.000
Slide	0.000	2.063	1.558	0.000	-0.036	-0.109
Rise	0.000	1.558	8.451	0.000	-0.044	-0.183
Tilt	-0.086	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.036	-0.044	0.000	0.024	0.012
Twist	0.000	-0.109	-0.183	0.000	0.012	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.358	0.000	0.000	-0.148	0.000	0.000
Slide	0.000	2.680	2.836	0.000	0.048	-0.051
Rise	0.000	2.836	11.775	0.000	0.118	-0.224
Tilt	-0.148	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.048	0.118	0.000	0.026	0.005
Twist	0.000	-0.051	-0.224	0.000	0.005	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.432	0.000	0.000	-0.127	0.000	0.000
Slide	0.000	2.892	3.020	0.000	0.021	-0.048
Rise	0.000	3.020	11.629	0.000	0.124	-0.189
Tilt	-0.127	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.021	0.124	0.000	0.027	0.009
Twist	0.000	-0.048	-0.189	0.000	0.009	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.953	0.000	0.000	-0.096	0.000	0.000
Slide	0.000	1.727	1.798	0.000	0.012	-0.084
Rise	0.000	1.798	7.987	0.000	-0.018	-0.172
Tilt	-0.096	0.000	0.000	0.031	0.000	0.000
Roll	0.000	0.012	-0.018	0.000	0.017	0.004
Twist	0.000	-0.084	-0.172	0.000	0.004	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.999	0.000	0.000	-0.122	0.000	0.000
Slide	0.000	1.820	1.914	0.000	-0.017	-0.115
Rise	0.000	1.914	9.091	0.000	-0.010	-0.175
Tilt	-0.122	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.017	-0.010	0.000	0.020	0.006
Twist	0.000	-0.115	-0.175	0.000	0.006	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.253	0.000	0.000	-0.066	0.000	0.000
Slide	0.000	3.869	2.658	0.000	0.008	-0.093
Rise	0.000	2.658	10.376	0.000	0.058	-0.220
Tilt	-0.066	0.000	0.000	0.044	0.000	0.000
Roll	0.000	0.008	0.058	0.000	0.029	0.012
Twist	0.000	-0.093	-0.220	0.000	0.012	0.044

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.417	0.000	0.000	-0.051	0.000	0.000
Slide	0.000	2.384	1.323	0.000	0.024	-0.125
Rise	0.000	1.323	7.945	0.000	-0.098	-0.215
Tilt	-0.051	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.024	-0.098	0.000	0.016	0.009
Twist	0.000	-0.125	-0.215	0.000	0.009	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.770	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	3.016	0.897	0.000	0.022	-0.122
Rise	0.000	0.897	8.564	0.000	-0.131	-0.265
Tilt	-0.059	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.022	-0.131	0.000	0.021	0.012
Twist	0.000	-0.122	-0.265	0.000	0.012	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.750	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	2.875	0.703	0.000	-0.065	-0.148
Rise	0.000	0.703	9.730	0.000	-0.034	-0.206
Tilt	-0.106	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.065	-0.034	0.000	0.022	0.009
Twist	0.000	-0.148	-0.206	0.000	0.009	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.900	0.000	0.000	-0.098	0.000	0.000
Slide	0.000	2.620	1.097	0.000	-0.054	-0.152
Rise	0.000	1.097	9.364	0.000	-0.010	-0.163
Tilt	-0.098	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.054	-0.010	0.000	0.022	0.011
Twist	0.000	-0.152	-0.163	0.000	0.011	0.043

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-agct.15.cte	0.000	0.000

	Mean	Stdev
-atat.5.cte	0.000	0.000

	Mean	Stdev
-catg.10.cte	0.000	0.000

	Mean	Stdev
-catg.17.cte	0.000	0.000

	Mean	Stdev
-gatc.13.cte	0.000	0.000

	Mean	Stdev
-gcgc.16.cte	0.000	0.000

	Mean	Stdev
-gcgc.9.cte	0.000	0.000

	Mean	Stdev
-ggcc.12.cte	0.000	0.000

	Mean	Stdev
-ggcc.8.cte	0.000	0.000

	Mean	Stdev
-gtac.2.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tgca.7.cte	0.000	0.000

	Mean	Stdev
-ttaa.3.cte	0.000	0.000

	Mean	Stdev
-ttaa.6.cte	0.000	0.000

	Mean	Stdev
rise-10-CATG	3.131	0.315

	Mean	Stdev
roll-10-CATG	6.109	6.893

	Mean	Stdev
shift-10-CATG	0.000	1.036

	Mean	Stdev
slide-10-CATG	-0.026	0.525

	Mean	Stdev
tilt-10-CATG	0.000	5.130

	Mean	Stdev
twist-10-CATG	31.998	7.748

	Mean	Stdev
rise-11-AGCT	3.183	0.286

	Mean	Stdev
roll-11-AGCT	2.332	5.617

	Mean	Stdev
shift-11-AGCT	0.000	0.957

	Mean	Stdev
slide-11-AGCT	-0.274	0.615

	Mean	Stdev
tilt-11-AGCT	0.000	5.212

	Mean	Stdev
twist-11-AGCT	32.588	6.463

	Mean	Stdev
rise-12-GGCC	3.488	0.319

	Mean	Stdev
roll-12-GGCC	3.608	6.065

	Mean	Stdev
shift-12-GGCC	0.000	0.944

	Mean	Stdev
slide-12-GGCC	-0.459	0.702

	Mean	Stdev
tilt-12-GGCC	0.000	5.240

	Mean	Stdev
twist-12-GGCC	34.575	5.537

	Mean	Stdev
rise-13-GATC	3.330	0.295

	Mean	Stdev
roll-13-GATC	1.950	5.648

	Mean	Stdev
shift-13-GATC	0.000	0.856

	Mean	Stdev
slide-13-GATC	-0.124	0.620

	Mean	Stdev
tilt-13-GATC	0.000	4.553

	Mean	Stdev
twist-13-GATC	36.115	6.073

	Mean	Stdev
rise-14-AATT	3.233	0.277

	Mean	Stdev
roll-14-AATT	0.913	5.507

	Mean	Stdev
shift-14-AATT	0.000	0.714

	Mean	Stdev
slide-14-AATT	-0.318	0.552

	Mean	Stdev
tilt-14-AATT	0.000	4.657

	Mean	Stdev
twist-14-AATT	34.040	5.517

	Mean	Stdev
rise-15-AGCT	3.412	0.287

	Mean	Stdev
roll-15-AGCT	2.618	5.448

	Mean	Stdev
shift-15-AGCT	0.000	0.696

	Mean	Stdev
slide-15-AGCT	-0.738	0.538

	Mean	Stdev
tilt-15-AGCT	0.000	4.825

	Mean	Stdev
twist-15-AGCT	35.214	5.051

	Mean	Stdev
rise-16-GCGC	3.449	0.278

	Mean	Stdev
roll-16-GCGC	-1.149	5.068

	Mean	Stdev
shift-16-GCGC	0.000	0.836

	Mean	Stdev
slide-16-GCGC	-0.481	0.544

	Mean	Stdev
tilt-16-GCGC	0.000	4.735

	Mean	Stdev
twist-16-GCGC	35.996	4.395

	Mean	Stdev
rise-17-CATG	3.217	0.307

	Mean	Stdev
roll-17-CATG	6.036	6.599

	Mean	Stdev
shift-17-CATG	0.000	1.119

	Mean	Stdev
slide-17-CATG	-0.061	0.520

	Mean	Stdev
tilt-17-CATG	0.000	5.215

	Mean	Stdev
twist-17-CATG	34.564	6.164

	Mean	Stdev
rise-2-GTAC	3.369	0.278

	Mean	Stdev
roll-2-GTAC	-1.210	4.938

	Mean	Stdev
shift-2-GTAC	0.000	0.712

	Mean	Stdev
slide-2-GTAC	-0.633	0.431

	Mean	Stdev
tilt-2-GTAC	0.000	3.792

	Mean	Stdev
twist-2-GTAC	32.449	4.238

	Mean	Stdev
rise-3-TTAA	3.257	0.260

	Mean	Stdev
roll-3-TTAA	0.745	5.480

	Mean	Stdev
shift-3-TTAA	0.000	1.177

	Mean	Stdev
slide-3-TTAA	0.112	0.505

	Mean	Stdev
tilt-3-TTAA	0.000	5.522

	Mean	Stdev
twist-3-TTAA	36.689	4.451

	Mean	Stdev
rise-4-TATA	3.280	0.312

	Mean	Stdev
roll-4-TATA	4.731	7.452

	Mean	Stdev
shift-4-TATA	0.000	1.364

	Mean	Stdev
slide-4-TATA	0.160	0.639

	Mean	Stdev
tilt-4-TATA	0.000	5.465

	Mean	Stdev
twist-4-TATA	36.014	6.738

	Mean	Stdev
rise-5-ATAT	3.314	0.260

	Mean	Stdev
roll-5-ATAT	-2.803	4.933

	Mean	Stdev
shift-5-ATAT	0.000	0.785

	Mean	Stdev
slide-5-ATAT	-0.887	0.418

	Mean	Stdev
tilt-5-ATAT	0.000	3.693

	Mean	Stdev
twist-5-ATAT	32.055	4.308

	Mean	Stdev
rise-6-TTAA	3.292	0.262

	Mean	Stdev
roll-6-TTAA	-0.468	5.487

	Mean	Stdev
shift-6-TTAA	0.000	1.006

	Mean	Stdev
slide-6-TTAA	-0.070	0.537

	Mean	Stdev
tilt-6-TTAA	0.000	5.516

	Mean	Stdev
twist-6-TTAA	37.810	4.427

	Mean	Stdev
rise-7-TGCA	3.090	0.317

	Mean	Stdev
roll-7-TGCA	7.984	6.677

	Mean	Stdev
shift-7-TGCA	0.000	0.951

	Mean	Stdev
slide-7-TGCA	-0.040	0.535

	Mean	Stdev
tilt-7-TGCA	0.000	4.913

	Mean	Stdev
twist-7-TGCA	29.236	7.439

	Mean	Stdev
rise-8-GGCC	3.396	0.291

	Mean	Stdev
roll-8-GGCC	3.229	5.568

	Mean	Stdev
shift-8-GGCC	0.000	0.964

	Mean	Stdev
slide-8-GGCC	-0.290	0.698

	Mean	Stdev
tilt-8-GGCC	0.000	4.817

	Mean	Stdev
twist-8-GGCC	36.070	5.058

	Mean	Stdev
rise-9-GCGC	3.429	0.286

	Mean	Stdev
roll-9-GCGC	-0.785	5.141

	Mean	Stdev
shift-9-GCGC	0.000	0.751

	Mean	Stdev
slide-9-GCGC	-0.538	0.528

	Mean	Stdev
tilt-9-GCGC	0.000	4.438

	Mean	Stdev
twist-9-GCGC	36.154	4.484

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