NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.086	0.000	0.000	-0.091	0.000	0.000
Slide	0.000	2.378	1.152	0.000	-0.071	-0.142
Rise	0.000	1.152	8.181	0.000	-0.032	-0.135
Tilt	-0.091	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.071	-0.032	0.000	0.021	0.007
Twist	0.000	-0.142	-0.135	0.000	0.007	0.031

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.825	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	3.849	3.088	0.000	0.032	-0.068
Rise	0.000	3.088	10.980	0.000	0.081	-0.196
Tilt	-0.037	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.032	0.081	0.000	0.024	0.007
Twist	0.000	-0.068	-0.196	0.000	0.007	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.722	0.000	0.000	-0.068	0.000	0.000
Slide	0.000	2.302	1.674	0.000	-0.063	-0.095
Rise	0.000	1.674	8.839	0.000	-0.061	-0.205
Tilt	-0.068	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.063	-0.061	0.000	0.020	0.008
Twist	0.000	-0.095	-0.205	0.000	0.008	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.992	0.000	0.000	-0.049	0.000	0.000
Slide	0.000	2.161	1.784	0.000	-0.051	-0.069
Rise	0.000	1.784	7.910	0.000	-0.061	-0.215
Tilt	-0.049	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.051	-0.061	0.000	0.019	0.006
Twist	0.000	-0.069	-0.215	0.000	0.006	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.849	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.251	1.517	0.000	-0.055	-0.137
Rise	0.000	1.517	9.131	0.000	-0.010	-0.172
Tilt	-0.084	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.055	-0.010	0.000	0.022	0.006
Twist	0.000	-0.137	-0.172	0.000	0.006	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.320	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	4.489	2.967	0.000	-0.023	-0.083
Rise	0.000	2.967	10.959	0.000	0.050	-0.147
Tilt	-0.002	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.023	0.050	0.000	0.027	0.011
Twist	0.000	-0.083	-0.147	0.000	0.011	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.715	0.000	0.000	-0.075	0.000	0.000
Slide	0.000	2.990	0.580	0.000	0.013	-0.150
Rise	0.000	0.580	8.223	0.000	-0.108	-0.243
Tilt	-0.075	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.013	-0.108	0.000	0.018	0.009
Twist	0.000	-0.150	-0.243	0.000	0.009	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.791	0.000	0.000	-0.066	0.000	0.000
Slide	0.000	2.230	1.465	0.000	0.014	-0.106
Rise	0.000	1.465	6.724	0.000	-0.100	-0.220
Tilt	-0.066	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.014	-0.100	0.000	0.017	0.008
Twist	0.000	-0.106	-0.220	0.000	0.008	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.932	0.000	0.000	-0.074	0.000	0.000
Slide	0.000	2.167	1.195	0.000	-0.041	-0.146
Rise	0.000	1.195	8.370	0.000	-0.057	-0.172
Tilt	-0.074	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.041	-0.057	0.000	0.022	0.011
Twist	0.000	-0.146	-0.172	0.000	0.011	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.092	0.000	0.000	-0.083	0.000	0.000
Slide	0.000	1.923	1.143	0.000	-0.027	-0.107
Rise	0.000	1.143	7.725	0.000	-0.039	-0.150
Tilt	-0.083	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.027	-0.039	0.000	0.021	0.010
Twist	0.000	-0.107	-0.150	0.000	0.010	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.095	0.000	0.000	-0.118	0.000	0.000
Slide	0.000	2.042	1.134	0.000	-0.042	-0.096
Rise	0.000	1.134	8.885	0.000	-0.051	-0.156
Tilt	-0.118	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.042	-0.051	0.000	0.022	0.010
Twist	0.000	-0.096	-0.156	0.000	0.010	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.030	0.000	0.000	-0.110	0.000	0.000
Slide	0.000	1.626	1.789	0.000	0.015	-0.069
Rise	0.000	1.789	8.110	0.000	-0.038	-0.208
Tilt	-0.110	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.015	-0.038	0.000	0.018	0.005
Twist	0.000	-0.069	-0.208	0.000	0.005	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.400	0.000	0.000	-0.047	0.000	0.000
Slide	0.000	2.298	1.196	0.000	0.022	-0.114
Rise	0.000	1.196	8.062	0.000	-0.100	-0.257
Tilt	-0.047	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.022	-0.100	0.000	0.017	0.012
Twist	0.000	-0.114	-0.257	0.000	0.012	0.029

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-acgt.6.cte	0.000	0.000

	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-agct.13.cte	0.000	0.000

	Mean	Stdev
-agct.3.cte	0.000	0.000

	Mean	Stdev
-atat.9.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-catg.7.cte	0.000	0.000

	Mean	Stdev
-gatc.12.cte	0.000	0.000

	Mean	Stdev
-gatc.14.cte	0.000	0.000

	Mean	Stdev
-gatc.5.cte	0.000	0.000

	Mean	Stdev
-ggcc.4.cte	0.000	0.000

	Mean	Stdev
-tata.10.cte	0.000	0.000

	Mean	Stdev
rise-10-TATA	3.243	0.319

	Mean	Stdev
roll-10-TATA	4.451	7.610

	Mean	Stdev
shift-10-TATA	0.000	1.367

	Mean	Stdev
slide-10-TATA	0.148	0.630

	Mean	Stdev
tilt-10-TATA	0.000	5.366

	Mean	Stdev
twist-10-TATA	36.760	7.021

	Mean	Stdev
rise-11-AGCT	3.215	0.297

	Mean	Stdev
roll-11-AGCT	4.426	5.833

	Mean	Stdev
shift-11-AGCT	0.000	1.004

	Mean	Stdev
slide-11-AGCT	-0.204	0.578

	Mean	Stdev
tilt-11-AGCT	0.000	4.685

	Mean	Stdev
twist-11-AGCT	31.618	6.166

	Mean	Stdev
rise-12-GATC	3.380	0.282

	Mean	Stdev
roll-12-GATC	1.244	5.677

	Mean	Stdev
shift-12-GATC	0.000	0.868

	Mean	Stdev
slide-12-GATC	-0.079	0.622

	Mean	Stdev
tilt-12-GATC	0.000	4.477

	Mean	Stdev
twist-12-GATC	37.784	5.357

	Mean	Stdev
rise-13-AGCT	3.368	0.332

	Mean	Stdev
roll-13-AGCT	3.670	5.812

	Mean	Stdev
shift-13-AGCT	0.000	0.796

	Mean	Stdev
slide-13-AGCT	-0.493	0.591

	Mean	Stdev
tilt-13-AGCT	0.000	4.137

	Mean	Stdev
twist-13-AGCT	31.899	5.741

	Mean	Stdev
rise-14-GATC	3.418	0.293

	Mean	Stdev
roll-14-GATC	0.151	5.748

	Mean	Stdev
shift-14-GATC	0.000	0.811

	Mean	Stdev
slide-14-GATC	-0.125	0.616

	Mean	Stdev
tilt-14-GATC	0.000	4.577

	Mean	Stdev
twist-14-GATC	39.274	4.666

	Mean	Stdev
rise-2-CATG	3.089	0.320

	Mean	Stdev
roll-2-CATG	5.183	6.681

	Mean	Stdev
shift-2-CATG	0.000	1.064

	Mean	Stdev
slide-2-CATG	0.167	0.572

	Mean	Stdev
tilt-2-CATG	0.000	5.340

	Mean	Stdev
twist-2-CATG	32.725	7.413

	Mean	Stdev
rise-3-AGCT	3.194	0.276

	Mean	Stdev
roll-3-AGCT	1.435	5.419

	Mean	Stdev
shift-3-AGCT	0.000	0.979

	Mean	Stdev
slide-3-AGCT	-0.185	0.631

	Mean	Stdev
tilt-3-AGCT	0.000	5.274

	Mean	Stdev
twist-3-AGCT	33.892	5.811

	Mean	Stdev
rise-4-GGCC	3.488	0.332

	Mean	Stdev
roll-4-GGCC	3.349	5.976

	Mean	Stdev
shift-4-GGCC	0.000	0.947

	Mean	Stdev
slide-4-GGCC	-0.437	0.711

	Mean	Stdev
tilt-4-GGCC	0.000	5.366

	Mean	Stdev
twist-4-GGCC	33.930	5.680

	Mean	Stdev
rise-5-GATC	3.284	0.273

	Mean	Stdev
roll-5-GATC	1.437	5.604

	Mean	Stdev
shift-5-GATC	0.000	0.922

	Mean	Stdev
slide-5-GATC	-0.008	0.591

	Mean	Stdev
tilt-5-GATC	0.000	5.194

	Mean	Stdev
twist-5-GATC	37.291	5.278

	Mean	Stdev
rise-6-ACGT	3.308	0.280

	Mean	Stdev
roll-6-ACGT	-1.594	5.353

	Mean	Stdev
shift-6-ACGT	0.000	0.862

	Mean	Stdev
slide-6-ACGT	-0.621	0.445

	Mean	Stdev
tilt-6-ACGT	0.000	3.952

	Mean	Stdev
twist-6-ACGT	31.751	4.819

	Mean	Stdev
rise-7-CATG	3.385	0.369

	Mean	Stdev
roll-7-CATG	7.975	6.894

	Mean	Stdev
shift-7-CATG	0.000	0.980

	Mean	Stdev
slide-7-CATG	-0.167	0.633

	Mean	Stdev
tilt-7-CATG	0.000	5.654

	Mean	Stdev
twist-7-CATG	34.527	7.467

	Mean	Stdev
rise-8-AATT	3.298	0.281

	Mean	Stdev
roll-8-AATT	0.510	5.540

	Mean	Stdev
shift-8-AATT	0.000	0.879

	Mean	Stdev
slide-8-AATT	-0.262	0.604

	Mean	Stdev
tilt-8-AATT	0.000	5.741

	Mean	Stdev
twist-8-AATT	36.051	5.240

	Mean	Stdev
rise-9-ATAT	3.270	0.263

	Mean	Stdev
roll-9-ATAT	-2.020	5.011

	Mean	Stdev
shift-9-ATAT	0.000	0.666

	Mean	Stdev
slide-9-ATAT	-0.818	0.403

	Mean	Stdev
tilt-9-ATAT	0.000	3.801

	Mean	Stdev
twist-9-ATAT	31.453	4.075

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3'	G	-	G	-	T	-	C	-	C	-	T	-	G	-	T	-	T	-	A	-	T	-	C	-	T	-	C	-	T	-	G	5'
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