NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20
5'	C	-	A	-	T	-	T	-	T	-	A	-	A	-	G	-	A	-	T	-	A	-	T	-	C	-	T	-	T	-	A	-	A	-	A	-	T	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	T	-	A	-	A	-	A	-	T	-	T	-	C	-	T	-	A	-	T	-	A	-	G	-	A	-	A	-	T	-	T	-	T	-	A	-	C	5'
	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.901	0.000	0.000	-0.058	0.000	0.000
Slide	0.000	2.725	1.221	0.000	-0.082	-0.159
Rise	0.000	1.221	9.240	0.000	-0.039	-0.183
Tilt	-0.058	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.082	-0.039	0.000	0.024	0.011
Twist	0.000	-0.159	-0.183	0.000	0.011	0.032

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[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.469	0.000	0.000	-0.085	0.000	0.000
Slide	0.000	3.600	1.328	0.000	-0.032	-0.247
Rise	0.000	1.328	8.729	0.000	-0.063	-0.173
Tilt	-0.085	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.032	-0.063	0.000	0.023	0.007
Twist	0.000	-0.247	-0.173	0.000	0.007	0.052

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.724	0.000	0.000	-0.063	0.000	0.000
Slide	0.000	2.749	0.811	0.000	-0.086	-0.189
Rise	0.000	0.811	10.021	0.000	-0.000	-0.148
Tilt	-0.063	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.086	-0.000	0.000	0.022	0.007
Twist	0.000	-0.189	-0.148	0.000	0.007	0.031

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.276	0.000	0.000	-0.056	0.000	0.000
Slide	0.000	2.226	1.601	0.000	-0.061	-0.080
Rise	0.000	1.601	7.802	0.000	-0.048	-0.164
Tilt	-0.056	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.061	-0.048	0.000	0.023	0.006
Twist	0.000	-0.080	-0.164	0.000	0.006	0.028

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.706	0.000	0.000	-0.007	0.000	0.000
Slide	0.000	4.994	3.107	0.000	-0.062	-0.100
Rise	0.000	3.107	12.321	0.000	0.064	-0.170
Tilt	-0.007	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.062	0.064	0.000	0.030	0.013
Twist	0.000	-0.100	-0.170	0.000	0.013	0.043

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.010	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	5.116	2.998	0.000	-0.008	-0.147
Rise	0.000	2.998	11.524	0.000	0.081	-0.174
Tilt	-0.043	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.008	0.081	0.000	0.032	0.007
Twist	0.000	-0.147	-0.174	0.000	0.007	0.053

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.088	0.000	0.000	-0.038	0.000	0.000
Slide	0.000	4.916	2.921	0.000	-0.016	-0.130
Rise	0.000	2.921	11.366	0.000	0.072	-0.168
Tilt	-0.038	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.016	0.072	0.000	0.034	0.007
Twist	0.000	-0.130	-0.168	0.000	0.007	0.050

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.804	0.000	0.000	0.006	0.000	0.000
Slide	0.000	5.039	2.933	0.000	-0.070	-0.082
Rise	0.000	2.933	11.811	0.000	0.057	-0.156
Tilt	0.006	0.000	0.000	0.048	0.000	0.000
Roll	0.000	-0.070	0.057	0.000	0.030	0.012
Twist	0.000	-0.082	-0.156	0.000	0.012	0.041

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.281	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	2.277	1.779	0.000	-0.068	-0.083
Rise	0.000	1.779	7.679	0.000	-0.061	-0.176
Tilt	-0.059	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.068	-0.061	0.000	0.025	0.006
Twist	0.000	-0.083	-0.176	0.000	0.006	0.028

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.444	0.000	0.000	-0.087	0.000	0.000
Slide	0.000	1.954	1.409	0.000	-0.009	-0.145
Rise	0.000	1.409	8.277	0.000	-0.038	-0.167
Tilt	-0.087	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.009	-0.038	0.000	0.020	0.008
Twist	0.000	-0.145	-0.167	0.000	0.008	0.043

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.606	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	2.050	1.146	0.000	0.042	-0.103
Rise	0.000	1.146	7.045	0.000	-0.074	-0.218
Tilt	-0.053	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.042	-0.074	0.000	0.016	0.009
Twist	0.000	-0.103	-0.218	0.000	0.009	0.032

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.398	0.000	0.000	-0.047	0.000	0.000
Slide	0.000	2.583	0.893	0.000	-0.001	-0.159
Rise	0.000	0.893	8.628	0.000	-0.134	-0.241
Tilt	-0.047	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.001	-0.134	0.000	0.018	0.013
Twist	0.000	-0.159	-0.241	0.000	0.013	0.031

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.415	0.000	0.000	-0.050	0.000	0.000
Slide	0.000	2.532	0.937	0.000	0.008	-0.147
Rise	0.000	0.937	8.270	0.000	-0.130	-0.231
Tilt	-0.050	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.008	-0.130	0.000	0.019	0.013
Twist	0.000	-0.147	-0.231	0.000	0.013	0.029

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.430	0.000	0.000	-0.093	0.000	0.000
Slide	0.000	2.053	1.320	0.000	-0.002	-0.148
Rise	0.000	1.320	7.897	0.000	-0.037	-0.162
Tilt	-0.093	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.002	-0.037	0.000	0.020	0.006
Twist	0.000	-0.148	-0.163	0.000	0.006	0.041

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.710	0.000	0.000	-0.072	0.000	0.000
Slide	0.000	2.773	0.724	0.000	-0.084	-0.174
Rise	0.000	0.724	9.640	0.000	-0.019	-0.152
Tilt	-0.072	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.084	-0.019	0.000	0.022	0.009
Twist	0.000	-0.174	-0.152	0.000	0.009	0.033

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.600	0.000	0.000	-0.089	0.000	0.000
Slide	0.000	3.720	1.087	0.000	-0.024	-0.263
Rise	0.000	1.086	7.996	0.000	-0.052	-0.134
Tilt	-0.089	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.024	-0.052	0.000	0.024	0.004
Twist	0.000	-0.263	-0.134	0.000	0.004	0.053

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.872	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	2.755	1.187	0.000	-0.064	-0.163
Rise	0.000	1.187	9.151	0.000	-0.047	-0.185
Tilt	-0.061	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.064	-0.047	0.000	0.024	0.011
Twist	0.000	-0.163	-0.185	0.000	0.011	0.032

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	Mean	Stdev
-aatt.16.cte	0.000	0.000

	Mean	Stdev
-aatt.17.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-agct.7.cte	0.000	0.000

	Mean	Stdev
-atat.11.cte	0.000	0.000

	Mean	Stdev
-atat.18.cte	0.000	0.000

	Mean	Stdev
-atat.2.cte	0.000	0.000

	Mean	Stdev
-atat.9.cte	0.000	0.000

	Mean	Stdev
-ctag.13.cte	0.000	0.000

	Mean	Stdev
-gatc.8.cte	0.000	0.000

	Mean	Stdev
-tata.10.cte	0.000	0.000

	Mean	Stdev
-tata.15.cte	0.000	0.000

	Mean	Stdev
-tata.5.cte	0.000	0.000

	Mean	Stdev
-tcga.12.cte	0.000	0.000

	Mean	Stdev
-ttaa.14.cte	0.000	0.000

	Mean	Stdev
-ttaa.3.cte	0.000	0.000

	Mean	Stdev
-ttaa.4.cte	0.000	0.000

	Mean	Stdev
rise-10-TATA	3.434	0.331

	Mean	Stdev
roll-10-TATA	2.636	7.526

	Mean	Stdev
shift-10-TATA	0.000	1.076

	Mean	Stdev
slide-10-TATA	-0.102	0.671

	Mean	Stdev
tilt-10-TATA	0.000	4.998

	Mean	Stdev
twist-10-TATA	40.106	5.781

	Mean	Stdev
rise-11-ATAT	3.196	0.249

	Mean	Stdev
roll-11-ATAT	-1.799	4.903

	Mean	Stdev
shift-11-ATAT	0.000	0.586

	Mean	Stdev
slide-11-ATAT	-0.790	0.383

	Mean	Stdev
tilt-11-ATAT	0.000	3.610

	Mean	Stdev
twist-11-ATAT	29.787	4.117

	Mean	Stdev
rise-12-TCGA	3.455	0.292

	Mean	Stdev
roll-12-TCGA	-0.258	5.627

	Mean	Stdev
shift-12-TCGA	0.000	0.706

	Mean	Stdev
slide-12-TCGA	-0.326	0.642

	Mean	Stdev
tilt-12-TCGA	0.000	4.576

	Mean	Stdev
twist-12-TCGA	39.766	4.565

	Mean	Stdev
rise-13-CTAG	3.417	0.318

	Mean	Stdev
roll-13-CTAG	3.431	5.146

	Mean	Stdev
shift-13-CTAG	0.000	0.704

	Mean	Stdev
slide-13-CTAG	-0.649	0.587

	Mean	Stdev
tilt-13-CTAG	0.000	4.259

	Mean	Stdev
twist-13-CTAG	33.259	5.148

	Mean	Stdev
rise-14-TTAA	3.170	0.257

	Mean	Stdev
roll-14-TTAA	0.237	5.594

	Mean	Stdev
shift-14-TTAA	0.000	1.046

	Mean	Stdev
slide-14-TTAA	-0.005	0.575

	Mean	Stdev
tilt-14-TTAA	0.000	5.067

	Mean	Stdev
twist-14-TTAA	34.456	5.431

	Mean	Stdev
rise-15-TATA	3.122	0.297

	Mean	Stdev
roll-15-TATA	6.388	7.705

	Mean	Stdev
shift-15-TATA	0.000	1.373

	Mean	Stdev
slide-15-TATA	0.242	0.649

	Mean	Stdev
tilt-15-TATA	0.000	5.391

	Mean	Stdev
twist-15-TATA	32.516	7.536

	Mean	Stdev
rise-16-AATT	3.254	0.269

	Mean	Stdev
roll-16-AATT	0.886	5.447

	Mean	Stdev
shift-16-AATT	0.000	0.853

	Mean	Stdev
slide-16-AATT	-0.176	0.558

	Mean	Stdev
tilt-16-AATT	0.000	4.323

	Mean	Stdev
twist-16-AATT	34.146	5.562

	Mean	Stdev
rise-17-AATT	3.372	0.271

	Mean	Stdev
roll-17-AATT	-0.362	5.159

	Mean	Stdev
shift-17-AATT	0.000	0.690

	Mean	Stdev
slide-17-AATT	-0.390	0.495

	Mean	Stdev
tilt-17-AATT	0.000	4.820

	Mean	Stdev
twist-17-AATT	38.361	4.187

	Mean	Stdev
rise-18-ATAT	3.299	0.251

	Mean	Stdev
roll-18-ATAT	-2.684	4.431

	Mean	Stdev
shift-18-ATAT	0.000	0.546

	Mean	Stdev
slide-18-ATAT	-0.818	0.377

	Mean	Stdev
tilt-18-ATAT	0.000	3.663

	Mean	Stdev
twist-18-ATAT	31.867	3.568

	Mean	Stdev
rise-2-ATAT	3.301	0.253

	Mean	Stdev
roll-2-ATAT	-2.806	4.284

	Mean	Stdev
shift-2-ATAT	0.000	0.534

	Mean	Stdev
slide-2-ATAT	-0.826	0.383

	Mean	Stdev
tilt-2-ATAT	0.000	3.690

	Mean	Stdev
twist-2-ATAT	31.827	3.635

	Mean	Stdev
rise-3-TTAA	3.375	0.279

	Mean	Stdev
roll-3-TTAA	-0.346	5.035

	Mean	Stdev
shift-3-TTAA	0.000	0.664

	Mean	Stdev
slide-3-TTAA	-0.429	0.495

	Mean	Stdev
tilt-3-TTAA	0.000	4.954

	Mean	Stdev
twist-3-TTAA	38.452	4.165

	Mean	Stdev
rise-4-TTAA	3.259	0.270

	Mean	Stdev
roll-4-TTAA	0.598	5.364

	Mean	Stdev
shift-4-TTAA	0.000	0.873

	Mean	Stdev
slide-4-TTAA	-0.185	0.552

	Mean	Stdev
tilt-4-TTAA	0.000	4.325

	Mean	Stdev
twist-4-TTAA	34.341	5.587

	Mean	Stdev
rise-5-TATA	3.132	0.304

	Mean	Stdev
roll-5-TATA	6.825	7.502

	Mean	Stdev
shift-5-TATA	0.000	1.349

	Mean	Stdev
slide-5-TATA	0.222	0.652

	Mean	Stdev
tilt-5-TATA	0.000	5.308

	Mean	Stdev
twist-5-TATA	32.608	7.652

	Mean	Stdev
rise-6-AATT	3.158	0.252

	Mean	Stdev
roll-6-AATT	-0.070	5.537

	Mean	Stdev
shift-6-AATT	0.000	1.007

	Mean	Stdev
slide-6-AATT	-0.091	0.624

	Mean	Stdev
tilt-6-AATT	0.000	5.155

	Mean	Stdev
twist-6-AATT	33.585	5.960

	Mean	Stdev
rise-7-AGCT	3.425	0.308

	Mean	Stdev
roll-7-AGCT	2.764	5.275

	Mean	Stdev
shift-7-AGCT	0.000	0.702

	Mean	Stdev
slide-7-AGCT	-0.719	0.582

	Mean	Stdev
tilt-7-AGCT	0.000	4.170

	Mean	Stdev
twist-7-AGCT	33.519	5.027

	Mean	Stdev
rise-8-GATC	3.458	0.287

	Mean	Stdev
roll-8-GATC	-0.156	5.602

	Mean	Stdev
shift-8-GATC	0.000	0.691

	Mean	Stdev
slide-8-GATC	-0.357	0.658

	Mean	Stdev
tilt-8-GATC	0.000	4.466

	Mean	Stdev
twist-8-GATC	39.627	4.478

	Mean	Stdev
rise-9-ATAT	3.196	0.252

	Mean	Stdev
roll-9-ATAT	-1.686	4.893

	Mean	Stdev
shift-9-ATAT	0.000	0.570

	Mean	Stdev
slide-9-ATAT	-0.798	0.379

	Mean	Stdev
tilt-9-ATAT	0.000	3.484

	Mean	Stdev
twist-9-ATAT	29.630	4.158

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20
5'	C	-	A	-	T	-	T	-	T	-	A	-	A	-	G	-	A	-	T	-	A	-	T	-	C	-	T	-	T	-	A	-	A	-	A	-	T	-	G	3'
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3'	G	-	T	-	A	-	A	-	A	-	T	-	T	-	C	-	T	-	A	-	T	-	A	-	G	-	A	-	A	-	T	-	T	-	T	-	A	-	C	5'
	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20
5'	C	-	A	-	T	-	T	-	T	-	A	-	A	-	G	-	A	-	T	-	A	-	T	-	C	-	T	-	T	-	A	-	A	-	A	-	T	-	G	3'
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3'	G	-	T	-	A	-	A	-	A	-	T	-	T	-	C	-	T	-	A	-	T	-	A	-	G	-	A	-	A	-	T	-	T	-	T	-	A	-	C	5'
	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20
5'	C	-	A	-	T	-	T	-	T	-	A	-	A	-	G	-	A	-	T	-	A	-	T	-	C	-	T	-	T	-	A	-	A	-	A	-	T	-	G	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	G	-	T	-	A	-	A	-	A	-	T	-	T	-	C	-	T	-	A	-	T	-	A	-	G	-	A	-	A	-	T	-	T	-	T	-	A	-	C	5'
	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21