NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.982	0.000	0.000	-0.110	0.000	0.000
Slide	0.000	2.843	1.084	0.000	-0.057	-0.173
Rise	0.000	1.084	9.135	0.000	-0.037	-0.176
Tilt	-0.110	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.057	-0.037	0.000	0.023	0.008
Twist	0.000	-0.173	-0.176	0.000	0.008	0.047

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.078	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	3.759	3.044	0.000	0.046	-0.147
Rise	0.000	3.044	10.560	0.000	0.112	-0.193
Tilt	-0.067	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.046	0.112	0.000	0.026	0.006
Twist	0.000	-0.147	-0.193	0.000	0.006	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.523	0.000	0.000	-0.006	0.000	0.000
Slide	0.000	4.663	3.096	0.000	-0.024	-0.120
Rise	0.000	3.096	11.839	0.000	0.045	-0.147
Tilt	-0.006	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.024	0.045	0.000	0.028	0.008
Twist	0.000	-0.120	-0.147	0.000	0.008	0.048

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.988	0.000	0.000	0.024	0.000	0.000
Slide	0.000	4.485	2.780	0.000	-0.057	-0.083
Rise	0.000	2.780	11.656	0.000	0.064	-0.174
Tilt	0.024	0.000	0.000	0.046	0.000	0.000
Roll	0.000	-0.057	0.064	0.000	0.026	0.012
Twist	0.000	-0.083	-0.174	0.000	0.012	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.017	0.000	0.000	0.022	0.000	0.000
Slide	0.000	4.874	3.045	0.000	-0.044	-0.074
Rise	0.000	3.045	12.103	0.000	0.070	-0.164
Tilt	0.022	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.044	0.070	0.000	0.027	0.012
Twist	0.000	-0.074	-0.164	0.000	0.012	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.606	0.000	0.000	-0.009	0.000	0.000
Slide	0.000	5.385	2.998	0.000	-0.003	-0.213
Rise	0.000	2.998	11.197	0.000	0.043	-0.177
Tilt	-0.009	0.000	0.000	0.050	0.000	0.000
Roll	0.000	-0.003	0.043	0.000	0.037	0.005
Twist	0.000	-0.213	-0.177	0.000	0.005	0.075

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.998	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	3.674	2.909	0.000	0.049	-0.085
Rise	0.000	2.909	10.583	0.000	0.107	-0.236
Tilt	-0.043	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.049	0.107	0.000	0.025	0.007
Twist	0.000	-0.085	-0.236	0.000	0.007	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.453	0.000	0.000	-0.050	0.000	0.000
Slide	0.000	2.264	1.308	0.000	0.021	-0.114
Rise	0.000	1.309	7.346	0.000	-0.093	-0.222
Tilt	-0.050	0.000	0.000	0.023	0.000	0.000
Roll	0.000	0.021	-0.093	0.000	0.016	0.010
Twist	0.000	-0.114	-0.222	0.000	0.010	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.407	0.000	0.000	-0.056	0.000	0.000
Slide	0.000	2.256	1.035	0.000	0.039	-0.099
Rise	0.000	1.035	7.342	0.000	-0.081	-0.223
Tilt	-0.056	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.039	-0.081	0.000	0.017	0.010
Twist	0.000	-0.099	-0.223	0.000	0.010	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.395	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	2.524	1.168	0.000	0.037	-0.112
Rise	0.000	1.168	7.570	0.000	-0.085	-0.240
Tilt	-0.043	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.037	-0.085	0.000	0.016	0.010
Twist	0.000	-0.112	-0.239	0.000	0.010	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.477	0.000	0.000	-0.050	0.000	0.000
Slide	0.000	2.075	1.315	0.000	0.021	-0.107
Rise	0.000	1.315	7.323	0.000	-0.096	-0.226
Tilt	-0.050	0.000	0.000	0.022	0.000	0.000
Roll	0.000	0.021	-0.096	0.000	0.018	0.012
Twist	0.000	-0.107	-0.226	0.000	0.012	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.483	0.000	0.000	-0.064	0.000	0.000
Slide	0.000	2.055	1.371	0.000	0.029	-0.107
Rise	0.000	1.371	7.309	0.000	-0.107	-0.234
Tilt	-0.064	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.029	-0.107	0.000	0.019	0.013
Twist	0.000	-0.107	-0.234	0.000	0.013	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.910	0.000	0.000	-0.098	0.000	0.000
Slide	0.000	2.498	1.020	0.000	-0.060	-0.143
Rise	0.000	1.020	8.658	0.000	-0.040	-0.160
Tilt	-0.098	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.060	-0.040	0.000	0.022	0.009
Twist	0.000	-0.143	-0.160	0.000	0.009	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.908	0.000	0.000	-0.110	0.000	0.000
Slide	0.000	2.814	0.889	0.000	-0.065	-0.161
Rise	0.000	0.889	9.312	0.000	-0.029	-0.165
Tilt	-0.110	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.065	-0.029	0.000	0.025	0.008
Twist	0.000	-0.161	-0.165	0.000	0.008	0.047

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-acgt.15.cte	0.000	0.000

	Mean	Stdev
-atat.10.cte	0.000	0.000

	Mean	Stdev
-atat.13.cte	0.000	0.000

	Mean	Stdev
-atat.4.cte	0.000	0.000

	Mean	Stdev
-atat.7.cte	0.000	0.000

	Mean	Stdev
-gtac.2.cte	0.000	0.000

	Mean	Stdev
-tata.12.cte	0.000	0.000

	Mean	Stdev
-tata.14.cte	0.000	0.000

	Mean	Stdev
-tata.3.cte	0.000	0.000

	Mean	Stdev
-tata.5.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
-ttaa.11.cte	0.000	0.000

	Mean	Stdev
-ttaa.8.cte	0.000	0.000

	Mean	Stdev
rise-10-ATAT	3.279	0.249

	Mean	Stdev
roll-10-ATAT	-2.547	4.761

	Mean	Stdev
shift-10-ATAT	0.000	0.621

	Mean	Stdev
slide-10-ATAT	-0.839	0.398

	Mean	Stdev
tilt-10-ATAT	0.000	3.638

	Mean	Stdev
twist-10-ATAT	31.940	3.739

	Mean	Stdev
rise-11-TTAA	3.291	0.272

	Mean	Stdev
roll-11-TTAA	0.767	5.530

	Mean	Stdev
shift-11-TTAA	0.000	1.000

	Mean	Stdev
slide-11-TTAA	-0.147	0.549

	Mean	Stdev
tilt-11-TTAA	0.000	5.555

	Mean	Stdev
twist-11-TTAA	37.065	4.375

	Mean	Stdev
rise-12-TATA	3.305	0.329

	Mean	Stdev
roll-12-TATA	5.985	7.696

	Mean	Stdev
shift-12-TATA	0.000	1.305

	Mean	Stdev
slide-12-TATA	-0.024	0.644

	Mean	Stdev
tilt-12-TATA	0.000	5.802

	Mean	Stdev
twist-12-TATA	35.108	6.971

	Mean	Stdev
rise-13-ATAT	3.174	0.261

	Mean	Stdev
roll-13-ATAT	-0.722	5.469

	Mean	Stdev
shift-13-ATAT	0.000	0.775

	Mean	Stdev
slide-13-ATAT	-0.719	0.403

	Mean	Stdev
tilt-13-ATAT	0.000	3.605

	Mean	Stdev
twist-13-ATAT	29.859	4.631

	Mean	Stdev
rise-14-TATA	3.256	0.324

	Mean	Stdev
roll-14-TATA	3.635	7.322

	Mean	Stdev
shift-14-TATA	0.000	1.415

	Mean	Stdev
slide-14-TATA	0.031	0.618

	Mean	Stdev
tilt-14-TATA	0.000	5.434

	Mean	Stdev
twist-14-TATA	36.461	6.136

	Mean	Stdev
rise-15-ACGT	3.349	0.278

	Mean	Stdev
roll-15-ACGT	-1.532	5.074

	Mean	Stdev
shift-15-ACGT	0.000	0.778

	Mean	Stdev
slide-15-ACGT	-0.523	0.473

	Mean	Stdev
tilt-15-ACGT	0.000	3.991

	Mean	Stdev
twist-15-ACGT	33.396	4.268

	Mean	Stdev
rise-2-GTAC	3.340	0.291

	Mean	Stdev
roll-2-GTAC	-1.424	5.246

	Mean	Stdev
shift-2-GTAC	0.000	0.786

	Mean	Stdev
slide-2-GTAC	-0.480	0.455

	Mean	Stdev
tilt-2-GTAC	0.000	4.125

	Mean	Stdev
twist-2-GTAC	32.607	4.696

	Mean	Stdev
rise-3-TATA	3.243	0.323

	Mean	Stdev
roll-3-TATA	3.295	7.543

	Mean	Stdev
shift-3-TATA	0.000	1.351

	Mean	Stdev
slide-3-TATA	0.041	0.593

	Mean	Stdev
tilt-3-TATA	0.000	5.345

	Mean	Stdev
twist-3-TATA	36.763	6.361

	Mean	Stdev
rise-4-ATAT	3.165	0.255

	Mean	Stdev
roll-4-ATAT	-0.415	5.339

	Mean	Stdev
shift-4-ATAT	0.000	0.763

	Mean	Stdev
slide-4-ATAT	-0.687	0.385

	Mean	Stdev
tilt-4-ATAT	0.000	3.695

	Mean	Stdev
twist-4-ATAT	29.456	4.584

	Mean	Stdev
rise-5-TATA	3.323	0.329

	Mean	Stdev
roll-5-TATA	6.188	7.589

	Mean	Stdev
shift-5-TATA	0.000	1.275

	Mean	Stdev
slide-5-TATA	-0.078	0.670

	Mean	Stdev
tilt-5-TATA	0.000	5.982

	Mean	Stdev
twist-5-TATA	35.266	6.671

	Mean	Stdev
rise-6-AATT	3.323	0.264

	Mean	Stdev
roll-6-AATT	0.581	5.199

	Mean	Stdev
shift-6-AATT	0.000	0.988

	Mean	Stdev
slide-6-AATT	-0.119	0.523

	Mean	Stdev
tilt-6-AATT	0.000	5.516

	Mean	Stdev
twist-6-AATT	37.566	4.140

	Mean	Stdev
rise-7-ATAT	3.360	0.250

	Mean	Stdev
roll-7-ATAT	-2.963	3.999

	Mean	Stdev
shift-7-ATAT	0.000	0.474

	Mean	Stdev
slide-7-ATAT	-0.951	0.374

	Mean	Stdev
tilt-7-ATAT	0.000	3.413

	Mean	Stdev
twist-7-ATAT	32.838	2.991

	Mean	Stdev
rise-8-TTAA	3.294	0.260

	Mean	Stdev
roll-8-TTAA	0.403	5.054

	Mean	Stdev
shift-8-TTAA	0.000	1.034

	Mean	Stdev
slide-8-TTAA	-0.054	0.518

	Mean	Stdev
tilt-8-TTAA	0.000	5.369

	Mean	Stdev
twist-8-TTAA	37.495	4.069

	Mean	Stdev
rise-9-TATA	3.329	0.334

	Mean	Stdev
roll-9-TATA	5.869	7.273

	Mean	Stdev
shift-9-TATA	0.000	1.338

	Mean	Stdev
slide-9-TATA	-0.005	0.681

	Mean	Stdev
tilt-9-TATA	0.000	5.744

	Mean	Stdev
twist-9-TATA	35.288	6.253

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