NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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5'	G	-	G	-	G	-	G	-	T	-	G	-	A	-	T	-	T	-	G	-	T	-	T	-	C	-	A	-	G	3'
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3'	C	-	C	-	C	-	C	-	A	-	C	-	T	-	A	-	A	-	C	-	A	-	A	-	G	-	T	-	C	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.482	0.000	0.000	-0.033	0.000	0.000
Slide	0.000	5.502	2.970	0.000	-0.047	-0.178
Rise	0.000	2.970	11.308	0.000	0.045	-0.168
Tilt	-0.033	0.000	0.000	0.051	0.000	0.000
Roll	0.000	-0.047	0.045	0.000	0.034	0.011
Twist	0.000	-0.178	-0.168	0.000	0.011	0.065

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.636	0.000	0.000	-0.071	0.000	0.000
Slide	0.000	2.437	0.700	0.000	0.017	-0.105
Rise	0.000	0.700	4.841	0.000	0.005	-0.054
Tilt	-0.071	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.017	0.005	0.000	0.010	-0.000
Twist	0.000	-0.105	-0.054	0.000	-0.000	0.011

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.824	0.000	0.000	-0.113	0.000	0.000
Slide	0.000	3.029	1.627	0.000	-0.044	-0.096
Rise	0.000	1.627	9.221	0.000	-0.028	-0.155
Tilt	-0.113	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.044	-0.028	0.000	0.023	0.010
Twist	0.000	-0.096	-0.155	0.000	0.010	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.822	0.000	0.000	-0.014	0.000	0.000
Slide	0.000	1.086	0.459	0.000	-0.028	-0.071
Rise	0.000	0.459	3.820	0.000	-0.027	-0.115
Tilt	-0.014	0.000	0.000	0.017	0.000	0.000
Roll	0.000	-0.028	-0.027	0.000	0.016	0.003
Twist	0.000	-0.071	-0.115	0.000	0.003	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.308	0.000	0.000	-0.101	0.000	0.000
Slide	0.000	1.773	1.718	0.000	0.003	-0.106
Rise	0.000	1.718	7.203	0.000	-0.021	-0.166
Tilt	-0.101	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.003	-0.021	0.000	0.019	0.004
Twist	0.000	-0.106	-0.166	0.000	0.004	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.943	0.000	0.000	-0.039	0.000	0.000
Slide	0.000	3.348	2.775	0.000	0.034	-0.069
Rise	0.000	2.775	10.374	0.000	0.078	-0.175
Tilt	-0.039	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.034	0.078	0.000	0.025	0.008
Twist	0.000	-0.069	-0.175	0.000	0.008	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.113	0.000	0.000	-0.050	0.000	0.000
Slide	0.000	4.068	3.243	0.000	0.038	-0.134
Rise	0.000	3.243	11.823	0.000	0.143	-0.216
Tilt	-0.050	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.038	0.144	0.000	0.027	0.009
Twist	0.000	-0.134	-0.216	0.000	0.009	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.731	0.000	0.000	-0.076	0.000	0.000
Slide	0.000	2.372	1.203	0.000	-0.050	-0.123
Rise	0.000	1.203	7.969	0.000	-0.064	-0.179
Tilt	-0.076	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.050	-0.064	0.000	0.021	0.010
Twist	0.000	-0.123	-0.179	0.000	0.010	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.659	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	3.027	0.593	0.000	0.014	-0.125
Rise	0.000	0.593	8.399	0.000	-0.132	-0.248
Tilt	-0.077	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.014	-0.132	0.000	0.020	0.010
Twist	0.000	-0.125	-0.248	0.000	0.010	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.863	0.000	0.000	-0.069	0.000	0.000
Slide	0.000	2.198	1.489	0.000	0.011	-0.101
Rise	0.000	1.489	6.942	0.000	-0.097	-0.222
Tilt	-0.069	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.011	-0.097	0.000	0.018	0.009
Twist	0.000	-0.101	-0.222	0.000	0.009	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.269	0.000	0.000	-0.070	0.000	0.000
Slide	0.000	2.739	1.246	0.000	-0.058	-0.163
Rise	0.000	1.246	8.437	0.000	-0.040	-0.170
Tilt	-0.070	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.058	-0.040	0.000	0.021	0.008
Twist	0.000	-0.163	-0.170	0.000	0.008	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.003	0.000	0.000	-0.112	0.000	0.000
Slide	0.000	2.791	1.051	0.000	-0.054	-0.182
Rise	0.000	1.051	8.858	0.000	-0.016	-0.153
Tilt	-0.112	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.054	-0.016	0.000	0.022	0.008
Twist	0.000	-0.182	-0.153	0.000	0.008	0.044

[Image courtesy of Curves+]

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	Mean	Stdev
-atat.7.cte	0.000	0.000

	Mean	Stdev
-catg.13.cte	0.000	0.000

	Mean	Stdev
-gatc.6.cte	0.000	0.000

	Mean	Stdev
-ggcc.2.cte	0.000	0.000

	Mean	Stdev
-ggcc.3.cte	0.000	0.000

	Mean	Stdev
-gtac.10.cte	0.000	0.000

	Mean	Stdev
-gtac.4.cte	0.000	0.000

	Mean	Stdev
-tcga.12.cte	0.000	0.000

	Mean	Stdev
-tgca.5.cte	0.000	0.000

	Mean	Stdev
-tgca.9.cte	0.000	0.000

	Mean	Stdev
-ttaa.11.cte	0.000	0.000

	Mean	Stdev
-ttaa.8.cte	0.000	0.000

	Mean	Stdev
rise-10-GTAC	3.406	0.282

	Mean	Stdev
roll-10-GTAC	-1.510	5.218

	Mean	Stdev
shift-10-GTAC	0.000	0.805

	Mean	Stdev
slide-10-GTAC	-0.715	0.477

	Mean	Stdev
tilt-10-GTAC	0.000	3.892

	Mean	Stdev
twist-10-GTAC	33.610	4.552

	Mean	Stdev
rise-11-TTAA	3.283	0.280

	Mean	Stdev
roll-11-TTAA	0.067	5.612

	Mean	Stdev
shift-11-TTAA	0.000	0.732

	Mean	Stdev
slide-11-TTAA	-0.374	0.554

	Mean	Stdev
tilt-11-TTAA	0.000	4.884

	Mean	Stdev
twist-11-TTAA	35.857	5.226

	Mean	Stdev
rise-12-TCGA	3.326	0.297

	Mean	Stdev
roll-12-TCGA	2.586	5.870

	Mean	Stdev
shift-12-TCGA	0.000	1.019

	Mean	Stdev
slide-12-TCGA	0.072	0.576

	Mean	Stdev
tilt-12-TCGA	0.000	4.698

	Mean	Stdev
twist-12-TCGA	34.248	6.272

	Mean	Stdev
rise-13-CATG	3.156	0.359

	Mean	Stdev
roll-13-CATG	6.570	7.617

	Mean	Stdev
shift-13-CATG	0.000	1.165

	Mean	Stdev
slide-13-CATG	0.261	0.654

	Mean	Stdev
tilt-13-CATG	0.000	5.913

	Mean	Stdev
twist-13-CATG	35.632	9.952

	Mean	Stdev
rise-2-GGCC	3.532	0.431

	Mean	Stdev
roll-2-GGCC	4.677	6.124

	Mean	Stdev
shift-2-GGCC	0.000	0.851

	Mean	Stdev
slide-2-GGCC	-0.344	0.849

	Mean	Stdev
tilt-2-GGCC	0.000	5.908

	Mean	Stdev
twist-2-GGCC	33.863	6.644

	Mean	Stdev
rise-3-GGCC	3.645	0.330

	Mean	Stdev
roll-3-GGCC	3.302	5.614

	Mean	Stdev
shift-3-GGCC	0.000	0.751

	Mean	Stdev
slide-3-GGCC	-0.796	0.702

	Mean	Stdev
tilt-3-GGCC	0.000	4.407

	Mean	Stdev
twist-3-GGCC	37.435	4.954

	Mean	Stdev
rise-4-GTAC	3.274	0.272

	Mean	Stdev
roll-4-GTAC	-2.054	5.352

	Mean	Stdev
shift-4-GTAC	0.000	0.746

	Mean	Stdev
slide-4-GTAC	-0.500	0.443

	Mean	Stdev
tilt-4-GTAC	0.000	3.832

	Mean	Stdev
twist-4-GTAC	32.973	4.724

	Mean	Stdev
rise-5-TGCA	3.108	0.314

	Mean	Stdev
roll-5-TGCA	8.208	6.369

	Mean	Stdev
shift-5-TGCA	0.000	1.151

	Mean	Stdev
slide-5-TGCA	-0.053	0.519

	Mean	Stdev
tilt-5-TGCA	0.000	5.643

	Mean	Stdev
twist-5-TGCA	29.853	6.809

	Mean	Stdev
rise-6-GATC	3.366	0.270

	Mean	Stdev
roll-6-GATC	1.081	5.426

	Mean	Stdev
shift-6-GATC	0.000	1.112

	Mean	Stdev
slide-6-GATC	0.157	0.476

	Mean	Stdev
tilt-6-GATC	0.000	5.273

	Mean	Stdev
twist-6-GATC	38.573	4.371

	Mean	Stdev
rise-7-ATAT	3.349	0.250

	Mean	Stdev
roll-7-ATAT	-2.947	4.318

	Mean	Stdev
shift-7-ATAT	0.000	0.488

	Mean	Stdev
slide-7-ATAT	-0.906	0.365

	Mean	Stdev
tilt-7-ATAT	0.000	3.421

	Mean	Stdev
twist-7-ATAT	32.774	3.243

	Mean	Stdev
rise-8-TTAA	3.333	0.267

	Mean	Stdev
roll-8-TTAA	-0.167	5.345

	Mean	Stdev
shift-8-TTAA	0.000	0.981

	Mean	Stdev
slide-8-TTAA	-0.114	0.542

	Mean	Stdev
tilt-8-TTAA	0.000	5.485

	Mean	Stdev
twist-8-TTAA	37.549	4.412

	Mean	Stdev
rise-9-TGCA	3.345	0.359

	Mean	Stdev
roll-9-TGCA	8.061	6.608

	Mean	Stdev
shift-9-TGCA	0.000	0.929

	Mean	Stdev
slide-9-TGCA	-0.255	0.622

	Mean	Stdev
tilt-9-TGCA	0.000	5.325

	Mean	Stdev
twist-9-TGCA	33.036	7.289

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5'	G	-	G	-	G	-	G	-	T	-	G	-	A	-	T	-	T	-	G	-	T	-	T	-	C	-	A	-	G	3'
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3'	C	-	C	-	C	-	C	-	A	-	C	-	T	-	A	-	A	-	C	-	A	-	A	-	G	-	T	-	C	5'
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