NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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3'	G	-	T	-	G	-	G	-	G	-	A	-	A	-	A	-	C	-	T	-	T	-	C	-	G	-	A	-	G	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.269	0.000	0.000	-0.016	0.000	0.000
Slide	0.000	2.913	0.882	0.000	-0.107	-0.129
Rise	0.000	0.882	9.161	0.000	-0.051	-0.173
Tilt	-0.016	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.107	-0.050	0.000	0.024	0.011
Twist	0.000	-0.129	-0.173	0.000	0.011	0.027

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.968	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	3.700	2.819	0.000	0.023	-0.104
Rise	0.000	2.819	9.730	0.000	0.157	-0.134
Tilt	-0.043	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.023	0.157	0.000	0.021	0.000
Twist	0.000	-0.104	-0.134	0.000	0.000	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.453	0.000	0.000	-0.136	0.000	0.000
Slide	0.000	2.803	1.625	0.000	-0.054	-0.156
Rise	0.000	1.625	8.144	0.000	-0.041	-0.150
Tilt	-0.136	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.054	-0.041	0.000	0.024	0.002
Twist	0.000	-0.156	-0.150	0.000	0.002	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.210	0.000	0.000	-0.109	0.000	0.000
Slide	0.000	1.466	1.724	0.000	0.004	-0.078
Rise	0.000	1.724	7.404	0.000	-0.033	-0.188
Tilt	-0.109	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.004	-0.033	0.000	0.020	0.004
Twist	0.000	-0.078	-0.188	0.000	0.004	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.213	0.000	0.000	-0.100	0.000	0.000
Slide	0.000	1.444	1.744	0.000	0.001	-0.081
Rise	0.000	1.744	8.213	0.000	-0.027	-0.178
Tilt	-0.100	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.001	-0.027	0.000	0.022	0.004
Twist	0.000	-0.081	-0.178	0.000	0.004	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.367	0.000	0.000	-0.104	0.000	0.000
Slide	0.000	2.671	1.665	0.000	-0.053	-0.125
Rise	0.000	1.665	7.792	0.000	-0.061	-0.169
Tilt	-0.104	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.053	-0.061	0.000	0.023	0.004
Twist	0.000	-0.125	-0.169	0.000	0.004	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.132	0.000	0.000	-0.062	0.000	0.000
Slide	0.000	2.088	1.662	0.000	-0.054	-0.068
Rise	0.000	1.662	8.045	0.000	-0.050	-0.175
Tilt	-0.062	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.054	-0.050	0.000	0.021	0.004
Twist	0.000	-0.068	-0.175	0.000	0.004	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.800	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.481	1.252	0.000	-0.053	-0.132
Rise	0.000	1.252	8.504	0.000	-0.034	-0.156
Tilt	-0.084	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.053	-0.034	0.000	0.022	0.011
Twist	0.000	-0.132	-0.156	0.000	0.011	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.572	0.000	0.000	-0.124	0.000	0.000
Slide	0.000	3.509	2.730	0.000	0.058	-0.182
Rise	0.000	2.730	11.866	0.000	0.124	-0.208
Tilt	-0.124	0.000	0.000	0.046	0.000	0.000
Roll	0.000	0.058	0.124	0.000	0.032	0.003
Twist	0.000	-0.182	-0.208	0.000	0.003	0.049

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.752	0.000	0.000	-0.063	0.000	0.000
Slide	0.000	2.699	0.681	0.000	0.007	-0.114
Rise	0.000	0.681	7.906	0.000	-0.140	-0.252
Tilt	-0.063	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.007	-0.140	0.000	0.020	0.011
Twist	0.000	-0.114	-0.252	0.000	0.011	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.205	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	2.732	1.020	0.000	-0.063	-0.118
Rise	0.000	1.020	8.843	0.000	-0.050	-0.156
Tilt	-0.053	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.063	-0.050	0.000	0.021	0.009
Twist	0.000	-0.118	-0.156	0.000	0.009	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.838	0.000	0.000	-0.069	0.000	0.000
Slide	0.000	2.719	1.172	0.000	-0.062	-0.177
Rise	0.000	1.172	9.060	0.000	-0.010	-0.182
Tilt	-0.069	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.062	-0.010	0.000	0.021	0.008
Twist	0.000	-0.177	-0.182	0.000	0.008	0.029

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-acgt.2.cte	0.000	0.000

	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-ccgg.3.cte	0.000	0.000

	Mean	Stdev
-ccgg.4.cte	0.000	0.000

	Mean	Stdev
-ctag.13.cte	0.000	0.000

	Mean	Stdev
-ctag.5.cte	0.000	0.000

	Mean	Stdev
-gatc.9.cte	0.000	0.000

	Mean	Stdev
-gcgc.12.cte	0.000	0.000

	Mean	Stdev
-tgca.8.cte	0.000	0.000

	Mean	Stdev
-ttaa.6.cte	0.000	0.000

	Mean	Stdev
-ttaa.7.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.207	0.270

	Mean	Stdev
roll-10-AATT	1.233	5.606

	Mean	Stdev
shift-10-AATT	0.000	0.682

	Mean	Stdev
slide-10-AATT	-0.393	0.524

	Mean	Stdev
tilt-10-AATT	0.000	4.483

	Mean	Stdev
twist-10-AATT	32.722	5.764

	Mean	Stdev
rise-11-AGCT	3.449	0.288

	Mean	Stdev
roll-11-AGCT	2.707	5.088

	Mean	Stdev
shift-11-AGCT	0.000	0.790

	Mean	Stdev
slide-11-AGCT	-0.648	0.556

	Mean	Stdev
tilt-11-AGCT	0.000	5.038

	Mean	Stdev
twist-11-AGCT	35.896	4.911

	Mean	Stdev
rise-12-GCGC	3.571	0.250

	Mean	Stdev
roll-12-GCGC	-0.149	4.430

	Mean	Stdev
shift-12-GCGC	0.000	0.512

	Mean	Stdev
slide-12-GCGC	-0.847	0.492

	Mean	Stdev
tilt-12-GCGC	0.000	3.816

	Mean	Stdev
twist-12-GCGC	37.249	3.935

	Mean	Stdev
rise-13-CTAG	3.406	0.303

	Mean	Stdev
roll-13-CTAG	2.488	5.159

	Mean	Stdev
shift-13-CTAG	0.000	0.746

	Mean	Stdev
slide-13-CTAG	-0.554	0.543

	Mean	Stdev
tilt-13-CTAG	0.000	4.700

	Mean	Stdev
twist-13-CTAG	34.439	5.005

	Mean	Stdev
rise-2-ACGT	3.269	0.302

	Mean	Stdev
roll-2-ACGT	-0.950	5.638

	Mean	Stdev
shift-2-ACGT	0.000	0.799

	Mean	Stdev
slide-2-ACGT	-0.483	0.476

	Mean	Stdev
tilt-2-ACGT	0.000	4.063

	Mean	Stdev
twist-2-ACGT	32.521	6.615

	Mean	Stdev
rise-3-CCGG	3.493	0.343

	Mean	Stdev
roll-3-CCGG	3.268	5.543

	Mean	Stdev
shift-3-CCGG	0.000	0.807

	Mean	Stdev
slide-3-CCGG	-0.707	0.765

	Mean	Stdev
tilt-3-CCGG	0.000	4.525

	Mean	Stdev
twist-3-CCGG	35.626	5.189

	Mean	Stdev
rise-4-CCGG	3.505	0.317

	Mean	Stdev
roll-4-CCGG	3.604	5.246

	Mean	Stdev
shift-4-CCGG	0.000	0.792

	Mean	Stdev
slide-4-CCGG	-0.732	0.767

	Mean	Stdev
tilt-4-CCGG	0.000	4.603

	Mean	Stdev
twist-4-CCGG	34.826	4.889

	Mean	Stdev
rise-5-CTAG	3.330	0.312

	Mean	Stdev
roll-5-CTAG	2.714	5.527

	Mean	Stdev
shift-5-CTAG	0.000	0.762

	Mean	Stdev
slide-5-CTAG	-0.604	0.603

	Mean	Stdev
tilt-5-CTAG	0.000	4.591

	Mean	Stdev
twist-5-CTAG	33.471	5.623

	Mean	Stdev
rise-6-TTAA	3.269	0.272

	Mean	Stdev
roll-6-TTAA	0.418	5.862

	Mean	Stdev
shift-6-TTAA	0.000	0.731

	Mean	Stdev
slide-6-TTAA	-0.294	0.518

	Mean	Stdev
tilt-6-TTAA	0.000	4.974

	Mean	Stdev
twist-6-TTAA	35.773	5.558

	Mean	Stdev
rise-7-TTAA	3.242	0.274

	Mean	Stdev
roll-7-TTAA	-0.047	5.735

	Mean	Stdev
shift-7-TTAA	0.000	0.914

	Mean	Stdev
slide-7-TTAA	-0.176	0.602

	Mean	Stdev
tilt-7-TTAA	0.000	4.509

	Mean	Stdev
twist-7-TTAA	34.048	6.288

	Mean	Stdev
rise-8-TGCA	3.095	0.339

	Mean	Stdev
roll-8-TGCA	8.599	6.675

	Mean	Stdev
shift-8-TGCA	0.000	0.986

	Mean	Stdev
slide-8-TGCA	0.061	0.558

	Mean	Stdev
tilt-8-TGCA	0.000	5.213

	Mean	Stdev
twist-8-TGCA	29.544	7.842

	Mean	Stdev
rise-9-GATC	3.329	0.280

	Mean	Stdev
roll-9-GATC	2.130	5.711

	Mean	Stdev
shift-9-GATC	0.000	0.995

	Mean	Stdev
slide-9-GATC	0.155	0.561

	Mean	Stdev
tilt-9-GATC	0.000	4.847

	Mean	Stdev
twist-9-GATC	36.514	5.595

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3'	G	-	T	-	G	-	G	-	G	-	A	-	A	-	A	-	C	-	T	-	T	-	C	-	G	-	A	-	G	5'
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