NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.837	0.000	0.000	-0.035	0.000	0.000
Slide	0.000	2.914	1.735	0.000	0.025	-0.060
Rise	0.000	1.735	5.359	0.000	0.058	-0.109
Tilt	-0.035	0.000	0.000	0.009	0.000	0.000
Roll	0.000	0.025	0.058	0.000	0.010	0.004
Twist	0.000	-0.060	-0.109	0.000	0.004	0.022

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.067	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	2.445	1.502	0.000	0.035	-0.079
Rise	0.000	1.502	5.520	0.000	0.034	-0.104
Tilt	-0.026	0.000	0.000	0.014	0.000	0.000
Roll	0.000	0.035	0.034	0.000	0.010	0.004
Twist	0.000	-0.079	-0.104	0.000	0.004	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.466	0.000	0.000	-0.024	0.000	0.000
Slide	0.000	1.864	-0.362	0.000	0.020	-0.037
Rise	0.000	-0.362	3.517	0.000	-0.016	-0.078
Tilt	-0.024	0.000	0.000	0.009	0.000	0.000
Roll	0.000	0.020	-0.016	0.000	0.006	0.002
Twist	0.000	-0.037	-0.078	0.000	0.002	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.641	0.000	0.000	-0.021	0.000	0.000
Slide	0.000	1.918	-0.234	0.000	0.023	-0.035
Rise	0.000	-0.234	4.050	0.000	-0.022	-0.091
Tilt	-0.021	0.000	0.000	0.008	0.000	0.000
Roll	0.000	0.023	-0.022	0.000	0.008	0.003
Twist	0.000	-0.035	-0.091	0.000	0.003	0.015

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.367	0.000	0.000	-0.025	0.000	0.000
Slide	0.000	1.478	1.200	0.000	-0.036	-0.066
Rise	0.000	1.200	3.322	0.000	-0.043	-0.066
Tilt	-0.025	0.000	0.000	0.009	0.000	0.000
Roll	0.000	-0.036	-0.043	0.000	0.010	0.005
Twist	0.000	-0.066	-0.066	0.000	0.005	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.685	0.000	0.000	-0.044	0.000	0.000
Slide	0.000	1.833	0.008	0.000	0.008	-0.049
Rise	0.000	0.008	3.400	0.000	-0.019	-0.070
Tilt	-0.044	0.000	0.000	0.011	0.000	0.000
Roll	0.000	0.008	-0.019	0.000	0.006	0.002
Twist	0.000	-0.049	-0.070	0.000	0.002	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.758	0.000	0.000	-0.046	0.000	0.000
Slide	0.000	1.993	-0.156	0.000	0.013	-0.058
Rise	0.000	-0.156	3.845	0.000	-0.021	-0.097
Tilt	-0.046	0.000	0.000	0.010	0.000	0.000
Roll	0.000	0.013	-0.021	0.000	0.008	0.003
Twist	0.000	-0.058	-0.097	0.000	0.003	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.193	0.000	0.000	-0.014	0.000	0.000
Slide	0.000	1.687	0.228	0.000	-0.001	-0.063
Rise	0.000	0.228	3.758	0.000	0.003	-0.040
Tilt	-0.014	0.000	0.000	0.008	0.000	0.000
Roll	0.000	-0.001	0.003	0.000	0.007	-0.000
Twist	0.000	-0.063	-0.040	0.000	-0.000	0.007

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.658	0.000	0.000	-0.035	0.000	0.000
Slide	0.000	1.993	-0.135	0.000	0.013	-0.061
Rise	0.000	-0.135	3.747	0.000	-0.020	-0.098
Tilt	-0.035	0.000	0.000	0.009	0.000	0.000
Roll	0.000	0.013	-0.020	0.000	0.008	0.002
Twist	0.000	-0.061	-0.098	0.000	0.002	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.688	0.000	0.000	-0.044	0.000	0.000
Slide	0.000	1.917	-0.116	0.000	0.007	-0.050
Rise	0.000	-0.116	4.079	0.000	-0.010	-0.082
Tilt	-0.044	0.000	0.000	0.011	0.000	0.000
Roll	0.000	0.007	-0.010	0.000	0.008	0.002
Twist	0.000	-0.050	-0.082	0.000	0.002	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.805	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	2.251	1.660	0.000	0.037	-0.030
Rise	0.000	1.660	5.697	0.000	0.099	-0.086
Tilt	-0.048	0.000	0.000	0.013	0.000	0.000
Roll	0.000	0.037	0.099	0.000	0.014	0.003
Twist	0.000	-0.030	-0.086	0.000	0.003	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.730	0.000	0.000	-0.044	0.000	0.000
Slide	0.000	2.516	1.304	0.000	0.039	-0.064
Rise	0.000	1.304	5.195	0.000	0.100	-0.078
Tilt	-0.044	0.000	0.000	0.014	0.000	0.000
Roll	0.000	0.039	0.100	0.000	0.011	0.002
Twist	0.000	-0.064	-0.078	0.000	0.002	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.041	0.000	0.000	-0.005	0.000	0.000
Slide	0.000	0.054	0.006	0.000	-0.005	-0.001
Rise	0.000	0.006	0.678	0.000	0.009	-0.002
Tilt	-0.005	0.000	0.000	0.004	0.000	0.000
Roll	0.000	-0.005	0.009	0.000	0.003	0.000
Twist	0.000	-0.001	-0.002	0.000	0.000	0.000

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.772	0.000	0.000	-0.049	0.000	0.000
Slide	0.000	2.450	1.418	0.000	0.058	-0.044
Rise	0.000	1.417	5.726	0.000	0.122	-0.084
Tilt	-0.049	0.000	0.000	0.015	0.000	0.000
Roll	0.000	0.058	0.122	0.000	0.013	0.002
Twist	0.000	-0.044	-0.084	0.000	0.002	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.767	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	2.526	1.722	0.000	0.040	-0.059
Rise	0.000	1.722	5.940	0.000	0.092	-0.103
Tilt	-0.048	0.000	0.000	0.013	0.000	0.000
Roll	0.000	0.040	0.092	0.000	0.013	0.002
Twist	0.000	-0.059	-0.103	0.000	0.002	0.020

[Image courtesy of Curves+]

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	Mean	Stdev
-acgt.11.cte	0.000	0.000

	Mean	Stdev
-acgt.8.cte	0.000	0.000

	Mean	Stdev
-catg.10.cte	0.000	0.000

	Mean	Stdev
-catg.7.cte	0.000	0.000

	Mean	Stdev
-ccgg.9.cte	0.000	0.000

	Mean	Stdev
-cgcg.12.cte	0.000	0.000

	Mean	Stdev
-cgcg.14.cte	0.000	0.000

	Mean	Stdev
-cgcg.16.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.5.cte	0.000	0.000

	Mean	Stdev
-gcgc.13.cte	0.000	0.000

	Mean	Stdev
-gcgc.15.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-gcgc.4.cte	0.000	0.000

	Mean	Stdev
-gcgc.6.cte	0.000	0.000

	Mean	Stdev
rise-10-CATG	3.293	0.441

	Mean	Stdev
roll-10-CATG	3.912	10.424

	Mean	Stdev
shift-10-CATG	0.000	1.207

	Mean	Stdev
slide-10-CATG	-0.068	0.607

	Mean	Stdev
tilt-10-CATG	0.000	8.643

	Mean	Stdev
twist-10-CATG	33.481	6.645

	Mean	Stdev
rise-11-ACGT	3.239	0.398

	Mean	Stdev
roll-11-ACGT	-0.442	8.456

	Mean	Stdev
shift-11-ACGT	0.000	0.912

	Mean	Stdev
slide-11-ACGT	-0.415	0.504

	Mean	Stdev
tilt-11-ACGT	0.000	8.608

	Mean	Stdev
twist-11-ACGT	30.740	5.887

	Mean	Stdev
rise-12-CGCG	3.402	0.435

	Mean	Stdev
roll-12-CGCG	4.913	10.119

	Mean	Stdev
shift-12-CGCG	0.000	1.084

	Mean	Stdev
slide-12-CGCG	0.193	0.594

	Mean	Stdev
tilt-12-CGCG	0.000	8.740

	Mean	Stdev
twist-12-CGCG	37.772	6.206

	Mean	Stdev
rise-13-GCGC	3.335	0.395

	Mean	Stdev
roll-13-GCGC	-0.521	7.131

	Mean	Stdev
shift-13-GCGC	0.000	0.965

	Mean	Stdev
slide-13-GCGC	-0.305	0.577

	Mean	Stdev
tilt-13-GCGC	0.000	7.527

	Mean	Stdev
twist-13-GCGC	33.741	5.655

	Mean	Stdev
rise-14-CGCG	3.268	0.431

	Mean	Stdev
roll-14-CGCG	5.664	8.714

	Mean	Stdev
shift-14-CGCG	0.000	1.028

	Mean	Stdev
slide-14-CGCG	0.075	0.593

	Mean	Stdev
tilt-14-CGCG	0.000	8.775

	Mean	Stdev
twist-14-CGCG	35.729	6.999

	Mean	Stdev
rise-15-GCGC	3.361	0.400

	Mean	Stdev
roll-15-GCGC	-0.697	8.463

	Mean	Stdev
shift-15-GCGC	0.000	0.994

	Mean	Stdev
slide-15-GCGC	-0.159	0.537

	Mean	Stdev
tilt-15-GCGC	0.000	7.179

	Mean	Stdev
twist-15-GCGC	32.823	5.725

	Mean	Stdev
rise-16-CGCG	3.166	0.410

	Mean	Stdev
roll-16-CGCG	6.724	9.360

	Mean	Stdev
shift-16-CGCG	0.000	1.854

	Mean	Stdev
slide-16-CGCG	0.280	0.738

	Mean	Stdev
tilt-16-CGCG	0.000	9.057

	Mean	Stdev
twist-16-CGCG	37.732	12.113

	Mean	Stdev
rise-2-GCGC	20.452	0.964

	Mean	Stdev
roll-2-GCGC	-3.276	14.352

	Mean	Stdev
shift-2-GCGC	0.000	4.065

	Mean	Stdev
slide-2-GCGC	-0.343	3.574

	Mean	Stdev
tilt-2-GCGC	0.000	13.330

	Mean	Stdev
twist-2-GCGC	38.509	14.097

	Mean	Stdev
rise-3-CGCG	3.238	0.439

	Mean	Stdev
roll-3-CGCG	5.572	8.824

	Mean	Stdev
shift-3-CGCG	0.000	1.059

	Mean	Stdev
slide-3-CGCG	0.012	0.598

	Mean	Stdev
tilt-3-CGCG	0.000	8.951

	Mean	Stdev
twist-3-CGCG	34.310	7.138

	Mean	Stdev
rise-4-GCGC	3.381	0.387

	Mean	Stdev
roll-4-GCGC	-1.912	7.912

	Mean	Stdev
shift-4-GCGC	0.000	0.976

	Mean	Stdev
slide-4-GCGC	-0.237	0.545

	Mean	Stdev
tilt-4-GCGC	0.000	6.968

	Mean	Stdev
twist-4-GCGC	33.566	5.546

	Mean	Stdev
rise-5-CGCG	3.226	0.404

	Mean	Stdev
roll-5-CGCG	4.944	8.662

	Mean	Stdev
shift-5-CGCG	0.000	1.076

	Mean	Stdev
slide-5-CGCG	0.053	0.587

	Mean	Stdev
tilt-5-CGCG	0.000	8.576

	Mean	Stdev
twist-5-CGCG	35.932	6.880

	Mean	Stdev
rise-6-GCGC	3.290	0.379

	Mean	Stdev
roll-6-GCGC	-0.122	7.430

	Mean	Stdev
shift-6-GCGC	0.000	0.990

	Mean	Stdev
slide-6-GCGC	-0.232	0.549

	Mean	Stdev
tilt-6-GCGC	0.000	7.458

	Mean	Stdev
twist-6-GCGC	33.752	5.942

	Mean	Stdev
rise-7-CATG	3.190	0.417

	Mean	Stdev
roll-7-CATG	5.798	9.647

	Mean	Stdev
shift-7-CATG	0.000	1.000

	Mean	Stdev
slide-7-CATG	-0.087	0.599

	Mean	Stdev
tilt-7-CATG	0.000	8.858

	Mean	Stdev
twist-7-CATG	32.945	7.578

	Mean	Stdev
rise-8-ACGT	3.429	0.365

	Mean	Stdev
roll-8-ACGT	-0.997	8.468

	Mean	Stdev
shift-8-ACGT	0.000	0.758

	Mean	Stdev
slide-8-ACGT	-0.565	0.572

	Mean	Stdev
tilt-8-ACGT	0.000	6.571

	Mean	Stdev
twist-8-ACGT	33.471	5.437

	Mean	Stdev
rise-9-CCGG	3.384	0.502

	Mean	Stdev
roll-9-CCGG	6.650	8.370

	Mean	Stdev
shift-9-CCGG	0.000	1.409

	Mean	Stdev
slide-9-CCGG	-0.280	0.791

	Mean	Stdev
tilt-9-CCGG	0.000	9.091

	Mean	Stdev
twist-9-CCGG	36.264	5.860

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