NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.659	0.000	0.000	-0.060	0.000	0.000
Slide	0.000	3.326	1.226	0.000	-0.063	-0.218
Rise	0.000	1.226	7.576	0.000	-0.058	-0.173
Tilt	-0.060	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.063	-0.058	0.000	0.022	0.010
Twist	0.000	-0.218	-0.173	0.000	0.010	0.042

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.106	0.000	0.000	-0.082	0.000	0.000
Slide	0.000	2.525	0.823	0.000	-0.081	-0.146
Rise	0.000	0.823	8.295	0.000	-0.008	-0.198
Tilt	-0.082	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.081	-0.008	0.000	0.022	0.012
Twist	0.000	-0.146	-0.198	0.000	0.012	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.638	0.000	0.000	-0.057	0.000	0.000
Slide	0.000	3.465	1.053	0.000	-0.065	-0.219
Rise	0.000	1.053	7.940	0.000	-0.052	-0.176
Tilt	-0.057	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.065	-0.052	0.000	0.022	0.010
Twist	0.000	-0.219	-0.176	0.000	0.010	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.259	0.000	0.000	-0.085	0.000	0.000
Slide	0.000	2.463	0.954	0.000	-0.076	-0.149
Rise	0.000	0.954	7.644	0.000	-0.003	-0.186
Tilt	-0.085	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.076	-0.003	0.000	0.022	0.012
Twist	0.000	-0.149	-0.186	0.000	0.012	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.690	0.000	0.000	-0.066	0.000	0.000
Slide	0.000	3.486	1.236	0.000	-0.072	-0.231
Rise	0.000	1.236	7.629	0.000	-0.067	-0.181
Tilt	-0.066	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.072	-0.067	0.000	0.022	0.010
Twist	0.000	-0.231	-0.181	0.000	0.010	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.118	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.533	0.775	0.000	-0.080	-0.156
Rise	0.000	0.775	7.709	0.000	-0.008	-0.192
Tilt	-0.084	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.080	-0.008	0.000	0.021	0.012
Twist	0.000	-0.156	-0.192	0.000	0.012	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.903	0.000	0.000	-0.036	0.000	0.000
Slide	0.000	3.388	2.559	0.000	0.047	-0.146
Rise	0.000	2.559	9.696	0.000	0.084	-0.207
Tilt	-0.036	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.047	0.084	0.000	0.023	0.009
Twist	0.000	-0.146	-0.207	0.000	0.009	0.043

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.421	0.000	0.000	-0.060	0.000	0.000
Slide	0.000	3.238	2.195	0.000	0.028	-0.131
Rise	0.000	2.195	9.358	0.000	0.058	-0.211
Tilt	-0.060	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.028	0.058	0.000	0.025	0.010
Twist	0.000	-0.131	-0.211	0.000	0.010	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.825	0.000	0.000	-0.023	0.000	0.000
Slide	0.000	2.792	2.241	0.000	0.035	-0.064
Rise	0.000	2.241	9.190	0.000	0.082	-0.180
Tilt	-0.023	0.000	0.000	0.035	0.000	0.000
Roll	0.000	0.035	0.082	0.000	0.021	0.008
Twist	0.000	-0.064	-0.180	0.000	0.008	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.902	0.000	0.000	-0.031	0.000	0.000
Slide	0.000	3.262	2.308	0.000	0.043	-0.151
Rise	0.000	2.308	9.556	0.000	0.080	-0.185
Tilt	-0.031	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.043	0.080	0.000	0.023	0.010
Twist	0.000	-0.151	-0.185	0.000	0.010	0.045

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.279	0.000	0.000	-0.018	0.000	0.000
Slide	0.000	1.747	1.212	0.000	-0.006	-0.038
Rise	0.000	1.212	6.388	0.000	-0.122	-0.230
Tilt	-0.018	0.000	0.000	0.023	0.000	0.000
Roll	0.000	-0.006	-0.122	0.000	0.021	0.009
Twist	0.000	-0.038	-0.230	0.000	0.009	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.260	0.000	0.000	0.009	0.000	0.000
Slide	0.000	0.594	-0.125	0.000	-0.000	0.012
Rise	0.000	-0.125	1.787	0.000	-0.028	-0.036
Tilt	0.009	0.000	0.000	0.009	0.000	0.000
Roll	0.000	-0.000	-0.028	0.000	0.004	0.000
Twist	0.000	0.012	-0.036	0.000	0.000	0.005

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.174	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	1.650	1.163	0.000	-0.003	-0.047
Rise	0.000	1.163	5.968	0.000	-0.112	-0.215
Tilt	-0.026	0.000	0.000	0.022	0.000	0.000
Roll	0.000	-0.003	-0.112	0.000	0.019	0.009
Twist	0.000	-0.047	-0.215	0.000	0.009	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.257	0.000	0.000	-0.012	0.000	0.000
Slide	0.000	1.787	1.285	0.000	-0.004	-0.039
Rise	0.000	1.285	6.680	0.000	-0.132	-0.240
Tilt	-0.012	0.000	0.000	0.023	0.000	0.000
Roll	0.000	-0.004	-0.132	0.000	0.022	0.010
Twist	0.000	-0.039	-0.240	0.000	0.010	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.476	0.000	0.000	-0.018	0.000	0.000
Slide	0.000	1.001	0.161	0.000	-0.002	0.006
Rise	0.000	0.161	3.749	0.000	-0.054	-0.082
Tilt	-0.018	0.000	0.000	0.016	0.000	0.000
Roll	0.000	-0.002	-0.054	0.000	0.008	0.001
Twist	0.000	0.006	-0.082	0.000	0.001	0.007

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.13.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.5.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-aatt.9.cte	0.000	0.000

	Mean	Stdev
-acgt.11.cte	0.000	0.000

	Mean	Stdev
-acgt.15.cte	0.000	0.000

	Mean	Stdev
-acgt.3.cte	0.000	0.000

	Mean	Stdev
-acgt.7.cte	0.000	0.000

	Mean	Stdev
-catg.12.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-catg.4.cte	0.000	0.000

	Mean	Stdev
-catg.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.16.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.351	0.294

	Mean	Stdev
roll-10-AATT	0.507	5.501

	Mean	Stdev
shift-10-AATT	0.000	0.619

	Mean	Stdev
slide-10-AATT	-0.404	0.520

	Mean	Stdev
tilt-10-AATT	0.000	4.770

	Mean	Stdev
twist-10-AATT	36.304	4.835

	Mean	Stdev
rise-11-ACGT	3.384	0.285

	Mean	Stdev
roll-11-ACGT	-1.276	5.657

	Mean	Stdev
shift-11-ACGT	0.000	0.821

	Mean	Stdev
slide-11-ACGT	-0.684	0.497

	Mean	Stdev
tilt-11-ACGT	0.000	3.889

	Mean	Stdev
twist-11-ACGT	32.954	4.427

	Mean	Stdev
rise-12-CATG	3.196	0.443

	Mean	Stdev
roll-12-CATG	11.824	6.191

	Mean	Stdev
shift-12-CATG	0.000	0.681

	Mean	Stdev
slide-12-CATG	-0.296	0.625

	Mean	Stdev
tilt-12-CATG	0.000	5.028

	Mean	Stdev
twist-12-CATG	26.594	8.915

	Mean	Stdev
rise-13-AATT	3.301	0.301

	Mean	Stdev
roll-13-AATT	0.937	6.029

	Mean	Stdev
shift-13-AATT	0.000	0.804

	Mean	Stdev
slide-13-AATT	-0.087	0.583

	Mean	Stdev
tilt-13-AATT	0.000	4.624

	Mean	Stdev
twist-13-AATT	34.717	6.670

	Mean	Stdev
rise-14-AATT	3.345	0.286

	Mean	Stdev
roll-14-AATT	0.558	5.547

	Mean	Stdev
shift-14-AATT	0.000	0.622

	Mean	Stdev
slide-14-AATT	-0.324	0.507

	Mean	Stdev
tilt-14-AATT	0.000	4.907

	Mean	Stdev
twist-14-AATT	36.434	4.932

	Mean	Stdev
rise-15-ACGT	3.433	0.285

	Mean	Stdev
roll-15-ACGT	-0.682	5.288

	Mean	Stdev
shift-15-ACGT	0.000	0.663

	Mean	Stdev
slide-15-ACGT	-0.715	0.487

	Mean	Stdev
tilt-15-ACGT	0.000	3.939

	Mean	Stdev
twist-15-ACGT	32.500	4.442

	Mean	Stdev
rise-16-CGCG	3.363	0.486

	Mean	Stdev
roll-16-CGCG	7.156	9.194

	Mean	Stdev
shift-16-CGCG	0.000	1.134

	Mean	Stdev
slide-16-CGCG	-0.072	0.774

	Mean	Stdev
tilt-16-CGCG	0.000	6.242

	Mean	Stdev
twist-16-CGCG	34.279	10.473

	Mean	Stdev
rise-2-CATG	3.233	0.652

	Mean	Stdev
roll-2-CATG	5.177	12.527

	Mean	Stdev
shift-2-CATG	0.000	1.533

	Mean	Stdev
slide-2-CATG	0.004	1.019

	Mean	Stdev
tilt-2-CATG	0.000	8.414

	Mean	Stdev
twist-2-CATG	30.723	11.300

	Mean	Stdev
rise-3-ACGT	3.304	0.311

	Mean	Stdev
roll-3-ACGT	-0.318	6.152

	Mean	Stdev
shift-3-ACGT	0.000	0.851

	Mean	Stdev
slide-3-ACGT	-0.541	0.517

	Mean	Stdev
tilt-3-ACGT	0.000	4.107

	Mean	Stdev
twist-3-ACGT	30.793	5.917

	Mean	Stdev
rise-4-CATG	3.271	0.446

	Mean	Stdev
roll-4-CATG	11.077	6.435

	Mean	Stdev
shift-4-CATG	0.000	0.716

	Mean	Stdev
slide-4-CATG	-0.279	0.650

	Mean	Stdev
tilt-4-CATG	0.000	5.205

	Mean	Stdev
twist-4-CATG	29.021	9.026

	Mean	Stdev
rise-5-AATT	3.338	0.308

	Mean	Stdev
roll-5-AATT	1.234	5.957

	Mean	Stdev
shift-5-AATT	0.000	0.749

	Mean	Stdev
slide-5-AATT	-0.111	0.590

	Mean	Stdev
tilt-5-AATT	0.000	4.586

	Mean	Stdev
twist-5-AATT	35.025	6.223

	Mean	Stdev
rise-6-AATT	3.359	0.294

	Mean	Stdev
roll-6-AATT	0.648	5.474

	Mean	Stdev
shift-6-AATT	0.000	0.619

	Mean	Stdev
slide-6-AATT	-0.410	0.517

	Mean	Stdev
tilt-6-AATT	0.000	4.883

	Mean	Stdev
twist-6-AATT	36.367	4.896

	Mean	Stdev
rise-7-ACGT	3.394	0.280

	Mean	Stdev
roll-7-ACGT	-1.489	5.740

	Mean	Stdev
shift-7-ACGT	0.000	0.817

	Mean	Stdev
slide-7-ACGT	-0.679	0.507

	Mean	Stdev
tilt-7-ACGT	0.000	3.837

	Mean	Stdev
twist-7-ACGT	33.016	4.410

	Mean	Stdev
rise-8-CATG	3.190	0.439

	Mean	Stdev
roll-8-CATG	11.911	5.999

	Mean	Stdev
shift-8-CATG	0.000	0.685

	Mean	Stdev
slide-8-CATG	-0.300	0.622

	Mean	Stdev
tilt-8-CATG	0.000	5.088

	Mean	Stdev
twist-8-CATG	26.406	8.918

	Mean	Stdev
rise-9-AATT	3.322	0.309

	Mean	Stdev
roll-9-AATT	0.786	6.124

	Mean	Stdev
shift-9-AATT	0.000	0.805

	Mean	Stdev
slide-9-AATT	-0.079	0.590

	Mean	Stdev
tilt-9-AATT	0.000	4.665

	Mean	Stdev
twist-9-AATT	35.117	6.417

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