NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.226	0.000	0.000	-0.031	0.000	0.000
Slide	0.000	1.957	1.547	0.000	-0.089	-0.082
Rise	0.000	1.547	6.223	0.000	-0.051	-0.143
Tilt	-0.031	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.089	-0.051	0.000	0.023	0.007
Twist	0.000	-0.082	-0.143	0.000	0.007	0.027

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.210	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	1.911	1.630	0.000	-0.088	-0.071
Rise	0.000	1.630	6.507	0.000	-0.062	-0.154
Tilt	-0.029	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.088	-0.062	0.000	0.023	0.006
Twist	0.000	-0.071	-0.154	0.000	0.006	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.307	0.000	0.000	-0.022	0.000	0.000
Slide	0.000	2.573	1.646	0.000	-0.084	-0.145
Rise	0.000	1.646	6.236	0.000	-0.030	-0.155
Tilt	-0.022	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.084	-0.030	0.000	0.023	0.007
Twist	0.000	-0.145	-0.155	0.000	0.007	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.961	0.000	0.000	-0.064	0.000	0.000
Slide	0.000	1.387	1.263	0.000	-0.051	-0.042
Rise	0.000	1.263	6.302	0.000	-0.041	-0.115
Tilt	-0.064	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.051	-0.041	0.000	0.021	0.006
Twist	0.000	-0.042	-0.115	0.000	0.006	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.194	0.000	0.000	-0.036	0.000	0.000
Slide	0.000	1.995	1.509	0.000	-0.089	-0.085
Rise	0.000	1.509	6.150	0.000	-0.048	-0.143
Tilt	-0.036	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.089	-0.048	0.000	0.023	0.007
Twist	0.000	-0.085	-0.143	0.000	0.007	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.193	0.000	0.000	-0.032	0.000	0.000
Slide	0.000	1.919	1.579	0.000	-0.084	-0.077
Rise	0.000	1.579	6.488	0.000	-0.043	-0.148
Tilt	-0.032	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.084	-0.043	0.000	0.023	0.006
Twist	0.000	-0.077	-0.148	0.000	0.006	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.209	0.000	0.000	-0.027	0.000	0.000
Slide	0.000	1.970	1.621	0.000	-0.088	-0.083
Rise	0.000	1.621	6.307	0.000	-0.052	-0.152
Tilt	-0.027	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.088	-0.052	0.000	0.023	0.007
Twist	0.000	-0.083	-0.152	0.000	0.007	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.150	0.000	0.000	-0.019	0.000	0.000
Slide	0.000	0.889	0.237	0.000	0.035	0.039
Rise	0.000	0.237	4.969	0.000	-0.033	-0.044
Tilt	-0.019	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.035	-0.033	0.000	0.017	0.004
Twist	0.000	0.039	-0.044	0.000	0.004	0.007

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.132	0.000	0.000	-0.033	0.000	0.000
Slide	0.000	2.207	1.422	0.000	-0.052	-0.216
Rise	0.000	1.422	7.455	0.000	-0.061	-0.174
Tilt	-0.033	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.052	-0.061	0.000	0.021	0.013
Twist	0.000	-0.216	-0.174	0.000	0.013	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.126	0.000	0.000	-0.033	0.000	0.000
Slide	0.000	2.139	1.419	0.000	-0.049	-0.199
Rise	0.000	1.419	7.616	0.000	-0.062	-0.185
Tilt	-0.033	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.049	-0.062	0.000	0.021	0.013
Twist	0.000	-0.199	-0.185	0.000	0.013	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.155	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	2.303	1.272	0.000	-0.047	-0.218
Rise	0.000	1.272	7.091	0.000	-0.055	-0.165
Tilt	-0.037	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.047	-0.055	0.000	0.020	0.013
Twist	0.000	-0.218	-0.165	0.000	0.013	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.148	0.000	0.000	-0.031	0.000	0.000
Slide	0.000	2.147	1.356	0.000	-0.052	-0.205
Rise	0.000	1.356	7.528	0.000	-0.056	-0.164
Tilt	-0.031	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.052	-0.056	0.000	0.020	0.012
Twist	0.000	-0.205	-0.164	0.000	0.012	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.159	0.000	0.000	-0.028	0.000	0.000
Slide	0.000	2.134	1.437	0.000	-0.049	-0.200
Rise	0.000	1.437	7.262	0.000	-0.057	-0.165
Tilt	-0.028	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.049	-0.057	0.000	0.021	0.012
Twist	0.000	-0.200	-0.165	0.000	0.012	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.143	0.000	0.000	-0.040	0.000	0.000
Slide	0.000	2.209	1.442	0.000	-0.051	-0.216
Rise	0.000	1.442	7.318	0.000	-0.058	-0.171
Tilt	-0.040	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.051	-0.058	0.000	0.020	0.013
Twist	0.000	-0.216	-0.171	0.000	0.013	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.708	0.000	0.000	-0.080	0.000	0.000
Slide	0.000	2.394	1.972	0.000	-0.037	-0.199
Rise	0.000	1.972	7.112	0.000	-0.040	-0.177
Tilt	-0.080	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.037	-0.040	0.000	0.023	0.009
Twist	0.000	-0.199	-0.177	0.000	0.009	0.041

[Image courtesy of Curves+]

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	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-agct.13.cte	0.000	0.000

	Mean	Stdev
-agct.15.cte	0.000	0.000

	Mean	Stdev
-agct.3.cte	0.000	0.000

	Mean	Stdev
-agct.5.cte	0.000	0.000

	Mean	Stdev
-agct.7.cte	0.000	0.000

	Mean	Stdev
-agct.9.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-gatc.10.cte	0.000	0.000

	Mean	Stdev
-gatc.12.cte	0.000	0.000

	Mean	Stdev
-gatc.14.cte	0.000	0.000

	Mean	Stdev
-gatc.4.cte	0.000	0.000

	Mean	Stdev
-gatc.6.cte	0.000	0.000

	Mean	Stdev
-gatc.8.cte	0.000	0.000

	Mean	Stdev
-ggcc.16.cte	0.000	0.000

	Mean	Stdev
rise-10-GATC	3.323	0.301

	Mean	Stdev
roll-10-GATC	3.441	5.929

	Mean	Stdev
shift-10-GATC	0.000	0.732

	Mean	Stdev
slide-10-GATC	-0.491	0.750

	Mean	Stdev
tilt-10-GATC	0.000	4.475

	Mean	Stdev
twist-10-GATC	33.887	5.741

	Mean	Stdev
rise-11-AGCT	3.366	0.354

	Mean	Stdev
roll-11-AGCT	5.092	5.613

	Mean	Stdev
shift-11-AGCT	0.000	0.700

	Mean	Stdev
slide-11-AGCT	-0.734	0.676

	Mean	Stdev
tilt-11-AGCT	0.000	4.259

	Mean	Stdev
twist-11-AGCT	30.291	5.242

	Mean	Stdev
rise-12-GATC	3.314	0.300

	Mean	Stdev
roll-12-GATC	3.489	5.968

	Mean	Stdev
shift-12-GATC	0.000	0.733

	Mean	Stdev
slide-12-GATC	-0.469	0.747

	Mean	Stdev
tilt-12-GATC	0.000	4.449

	Mean	Stdev
twist-12-GATC	33.702	6.068

	Mean	Stdev
rise-13-AGCT	3.334	0.354

	Mean	Stdev
roll-13-AGCT	5.509	5.710

	Mean	Stdev
shift-13-AGCT	0.000	0.704

	Mean	Stdev
slide-13-AGCT	-0.647	0.684

	Mean	Stdev
tilt-13-AGCT	0.000	4.281

	Mean	Stdev
twist-13-AGCT	30.191	5.528

	Mean	Stdev
rise-14-GATC	3.319	0.305

	Mean	Stdev
roll-14-GATC	2.644	6.132

	Mean	Stdev
shift-14-GATC	0.000	0.727

	Mean	Stdev
slide-14-GATC	-0.450	0.725

	Mean	Stdev
tilt-14-GATC	0.000	4.517

	Mean	Stdev
twist-14-GATC	34.235	5.809

	Mean	Stdev
rise-15-AGCT	3.372	0.342

	Mean	Stdev
roll-15-AGCT	4.047	5.392

	Mean	Stdev
shift-15-AGCT	0.000	0.674

	Mean	Stdev
slide-15-AGCT	-0.831	0.594

	Mean	Stdev
tilt-15-AGCT	0.000	4.267

	Mean	Stdev
twist-15-AGCT	31.315	4.695

	Mean	Stdev
rise-16-GGCC	3.565	0.327

	Mean	Stdev
roll-16-GGCC	4.914	5.321

	Mean	Stdev
shift-16-GGCC	0.000	0.620

	Mean	Stdev
slide-16-GGCC	-1.096	0.693

	Mean	Stdev
tilt-16-GGCC	0.000	4.330

	Mean	Stdev
twist-16-GGCC	32.945	5.135

	Mean	Stdev
rise-2-CATG	3.199	0.367

	Mean	Stdev
roll-2-CATG	9.350	6.368

	Mean	Stdev
shift-2-CATG	0.000	0.718

	Mean	Stdev
slide-2-CATG	-0.076	0.974

	Mean	Stdev
tilt-2-CATG	0.000	4.679

	Mean	Stdev
twist-2-CATG	29.676	10.977

	Mean	Stdev
rise-3-AGCT	3.358	0.353

	Mean	Stdev
roll-3-AGCT	4.998	5.760

	Mean	Stdev
shift-3-AGCT	0.000	0.846

	Mean	Stdev
slide-3-AGCT	-0.468	0.750

	Mean	Stdev
tilt-3-AGCT	0.000	4.873

	Mean	Stdev
twist-3-AGCT	30.988	6.851

	Mean	Stdev
rise-4-GATC	3.334	0.298

	Mean	Stdev
roll-4-GATC	3.208	5.943

	Mean	Stdev
shift-4-GATC	0.000	0.724

	Mean	Stdev
slide-4-GATC	-0.414	0.739

	Mean	Stdev
tilt-4-GATC	0.000	4.376

	Mean	Stdev
twist-4-GATC	34.397	5.657

	Mean	Stdev
rise-5-AGCT	3.393	0.353

	Mean	Stdev
roll-5-AGCT	5.346	5.579

	Mean	Stdev
shift-5-AGCT	0.000	0.713

	Mean	Stdev
slide-5-AGCT	-0.751	0.666

	Mean	Stdev
tilt-5-AGCT	0.000	4.286

	Mean	Stdev
twist-5-AGCT	30.507	5.190

	Mean	Stdev
rise-6-GATC	3.312	0.306

	Mean	Stdev
roll-6-GATC	3.615	5.964

	Mean	Stdev
shift-6-GATC	0.000	0.719

	Mean	Stdev
slide-6-GATC	-0.499	0.742

	Mean	Stdev
tilt-6-GATC	0.000	4.475

	Mean	Stdev
twist-6-GATC	33.776	5.693

	Mean	Stdev
rise-7-AGCT	3.356	0.349

	Mean	Stdev
roll-7-AGCT	5.244	5.582

	Mean	Stdev
shift-7-AGCT	0.000	0.710

	Mean	Stdev
slide-7-AGCT	-0.739	0.680

	Mean	Stdev
tilt-7-AGCT	0.000	4.296

	Mean	Stdev
twist-7-AGCT	30.072	5.212

	Mean	Stdev
rise-8-GATC	3.326	0.304

	Mean	Stdev
roll-8-GATC	3.366	6.030

	Mean	Stdev
shift-8-GATC	0.000	0.733

	Mean	Stdev
slide-8-GATC	-0.491	0.751

	Mean	Stdev
tilt-8-GATC	0.000	4.536

	Mean	Stdev
twist-8-GATC	33.956	5.765

	Mean	Stdev
rise-9-AGCT	3.356	0.356

	Mean	Stdev
roll-9-AGCT	4.950	5.579

	Mean	Stdev
shift-9-AGCT	0.000	0.703

	Mean	Stdev
slide-9-AGCT	-0.736	0.677

	Mean	Stdev
tilt-9-AGCT	0.000	4.332

	Mean	Stdev
twist-9-AGCT	30.155	5.252

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