NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.393	0.000	0.000	-0.028	0.000	0.000
Slide	0.000	2.261	1.064	0.000	-0.096	-0.132
Rise	0.000	1.064	8.150	0.000	-0.060	-0.181
Tilt	-0.028	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.096	-0.059	0.000	0.023	0.012
Twist	0.000	-0.132	-0.181	0.000	0.012	0.029

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.538	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	2.394	1.179	0.000	-0.104	-0.106
Rise	0.000	1.179	7.835	0.000	-0.066	-0.135
Tilt	-0.002	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.104	-0.066	0.000	0.024	0.012
Twist	0.000	-0.106	-0.135	0.000	0.012	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.408	0.000	0.000	-0.027	0.000	0.000
Slide	0.000	2.361	1.083	0.000	-0.098	-0.133
Rise	0.000	1.083	7.708	0.000	-0.060	-0.164
Tilt	-0.027	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.098	-0.060	0.000	0.023	0.012
Twist	0.000	-0.133	-0.164	0.000	0.012	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.250	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	2.208	1.529	0.000	-0.073	-0.113
Rise	0.000	1.529	7.305	0.000	-0.049	-0.176
Tilt	-0.061	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.073	-0.049	0.000	0.020	0.007
Twist	0.000	-0.113	-0.176	0.000	0.007	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.658	0.000	0.000	0.019	0.000	0.000
Slide	0.000	1.826	0.740	0.000	-0.058	-0.068
Rise	0.000	0.740	4.156	0.000	-0.090	-0.171
Tilt	0.019	0.000	0.000	0.015	0.000	0.000
Roll	0.000	-0.058	-0.090	0.000	0.014	0.001
Twist	0.000	-0.068	-0.171	0.000	0.001	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.328	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.527	1.640	0.000	-0.087	-0.160
Rise	0.000	1.640	6.951	0.000	-0.048	-0.170
Tilt	-0.077	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.087	-0.048	0.000	0.021	0.006
Twist	0.000	-0.160	-0.170	0.000	0.006	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.290	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	2.450	1.807	0.000	-0.078	-0.150
Rise	0.000	1.807	7.385	0.000	-0.050	-0.188
Tilt	-0.067	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.078	-0.050	0.000	0.020	0.007
Twist	0.000	-0.150	-0.188	0.000	0.007	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.180	0.000	0.000	-0.112	0.000	0.000
Slide	0.000	2.148	0.577	0.000	0.021	-0.057
Rise	0.000	0.577	6.738	0.000	-0.084	-0.227
Tilt	-0.112	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.021	-0.084	0.000	0.017	0.006
Twist	0.000	-0.057	-0.227	0.000	0.006	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.214	0.000	0.000	-0.052	0.000	0.000
Slide	0.000	1.654	1.471	0.000	-0.042	-0.105
Rise	0.000	1.471	7.355	0.000	-0.048	-0.166
Tilt	-0.052	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.042	-0.048	0.000	0.021	0.012
Twist	0.000	-0.105	-0.166	0.000	0.012	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.238	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	1.698	1.055	0.000	-0.032	-0.121
Rise	0.000	1.055	7.205	0.000	-0.025	-0.159
Tilt	-0.067	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.032	-0.025	0.000	0.022	0.011
Twist	0.000	-0.121	-0.159	0.000	0.011	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.930	0.000	0.000	-0.075	0.000	0.000
Slide	0.000	1.864	1.288	0.000	-0.044	-0.112
Rise	0.000	1.288	7.323	0.000	-0.040	-0.173
Tilt	-0.075	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.044	-0.040	0.000	0.021	0.012
Twist	0.000	-0.112	-0.173	0.000	0.012	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.199	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	1.717	1.505	0.000	-0.047	-0.112
Rise	0.000	1.505	7.317	0.000	-0.052	-0.173
Tilt	-0.053	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.047	-0.052	0.000	0.021	0.012
Twist	0.000	-0.112	-0.173	0.000	0.012	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.300	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	1.494	1.727	0.000	-0.022	-0.089
Rise	0.000	1.727	6.880	0.000	-0.044	-0.179
Tilt	-0.077	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.022	-0.044	0.000	0.019	0.008
Twist	0.000	-0.089	-0.179	0.000	0.008	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.341	0.000	0.000	-0.085	0.000	0.000
Slide	0.000	1.437	1.706	0.000	-0.017	-0.079
Rise	0.000	1.706	6.755	0.000	-0.036	-0.171
Tilt	-0.085	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.017	-0.036	0.000	0.020	0.007
Twist	0.000	-0.079	-0.171	0.000	0.007	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.406	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	1.452	1.736	0.000	-0.029	-0.085
Rise	0.000	1.736	6.978	0.000	-0.048	-0.194
Tilt	-0.081	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.029	-0.048	0.000	0.021	0.009
Twist	0.000	-0.085	-0.194	0.000	0.009	0.028

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.12.cte	0.000	0.000

	Mean	Stdev
-aatt.4.cte	0.000	0.000

	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-agct.13.cte	0.000	0.000

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-agct.5.cte	0.000	0.000

	Mean	Stdev
-agct.9.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-gatc.11.cte	0.000	0.000

	Mean	Stdev
-gatc.15.cte	0.000	0.000

	Mean	Stdev
-gatc.3.cte	0.000	0.000

	Mean	Stdev
-gatc.7.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.490	0.357

	Mean	Stdev
roll-10-GGCC	6.044	5.911

	Mean	Stdev
shift-10-GGCC	0.000	0.731

	Mean	Stdev
slide-10-GGCC	-0.858	0.780

	Mean	Stdev
tilt-10-GGCC	0.000	4.892

	Mean	Stdev
twist-10-GGCC	31.651	5.932

	Mean	Stdev
rise-11-GATC	3.271	0.320

	Mean	Stdev
roll-11-GATC	3.520	6.171

	Mean	Stdev
shift-11-GATC	0.000	0.722

	Mean	Stdev
slide-11-GATC	-0.264	0.724

	Mean	Stdev
tilt-11-GATC	0.000	4.548

	Mean	Stdev
twist-11-GATC	33.757	6.540

	Mean	Stdev
rise-12-AATT	3.196	0.290

	Mean	Stdev
roll-12-AATT	1.682	5.886

	Mean	Stdev
shift-12-AATT	0.000	0.656

	Mean	Stdev
slide-12-AATT	-0.429	0.614

	Mean	Stdev
tilt-12-AATT	0.000	4.772

	Mean	Stdev
twist-12-AATT	32.216	5.821

	Mean	Stdev
rise-13-AGCT	3.322	0.318

	Mean	Stdev
roll-13-AGCT	3.918	5.773

	Mean	Stdev
shift-13-AGCT	0.000	0.723

	Mean	Stdev
slide-13-AGCT	-0.723	0.618

	Mean	Stdev
tilt-13-AGCT	0.000	4.761

	Mean	Stdev
twist-13-AGCT	32.594	5.409

	Mean	Stdev
rise-14-GGCC	3.521	0.364

	Mean	Stdev
roll-14-GGCC	5.931	5.780

	Mean	Stdev
shift-14-GGCC	0.000	0.731

	Mean	Stdev
slide-14-GGCC	-0.830	0.791

	Mean	Stdev
tilt-14-GGCC	0.000	4.957

	Mean	Stdev
twist-14-GGCC	31.806	5.992

	Mean	Stdev
rise-15-GATC	3.298	0.309

	Mean	Stdev
roll-15-GATC	1.953	5.785

	Mean	Stdev
shift-15-GATC	0.000	0.735

	Mean	Stdev
slide-15-GATC	-0.301	0.708

	Mean	Stdev
tilt-15-GATC	0.000	4.779

	Mean	Stdev
twist-15-GATC	35.059	6.049

	Mean	Stdev
rise-16-AGCT	3.318	0.514

	Mean	Stdev
roll-16-AGCT	2.440	7.587

	Mean	Stdev
shift-16-AGCT	0.000	0.961

	Mean	Stdev
slide-16-AGCT	-0.691	0.653

	Mean	Stdev
tilt-16-AGCT	0.000	6.334

	Mean	Stdev
twist-16-AGCT	31.890	7.213

	Mean	Stdev
rise-2-CGCG	3.128	0.404

	Mean	Stdev
roll-2-CGCG	8.490	6.455

	Mean	Stdev
shift-2-CGCG	0.000	0.903

	Mean	Stdev
slide-2-CGCG	0.051	0.569

	Mean	Stdev
tilt-2-CGCG	0.000	5.946

	Mean	Stdev
twist-2-CGCG	29.712	9.053

	Mean	Stdev
rise-3-GATC	3.376	0.317

	Mean	Stdev
roll-3-GATC	2.074	6.174

	Mean	Stdev
shift-3-GATC	0.000	0.881

	Mean	Stdev
slide-3-GATC	0.099	0.666

	Mean	Stdev
tilt-3-GATC	0.000	4.743

	Mean	Stdev
twist-3-GATC	35.994	6.742

	Mean	Stdev
rise-4-AATT	3.242	0.290

	Mean	Stdev
roll-4-AATT	1.992	5.825

	Mean	Stdev
shift-4-AATT	0.000	0.617

	Mean	Stdev
slide-4-AATT	-0.394	0.577

	Mean	Stdev
tilt-4-AATT	0.000	4.745

	Mean	Stdev
twist-4-AATT	32.958	5.260

	Mean	Stdev
rise-5-AGCT	3.415	0.321

	Mean	Stdev
roll-5-AGCT	4.306	5.699

	Mean	Stdev
shift-5-AGCT	0.000	0.717

	Mean	Stdev
slide-5-AGCT	-0.804	0.621

	Mean	Stdev
tilt-5-AGCT	0.000	4.665

	Mean	Stdev
twist-5-AGCT	33.122	4.980

	Mean	Stdev
rise-6-GGCC	3.502	0.360

	Mean	Stdev
roll-6-GGCC	6.234	5.685

	Mean	Stdev
shift-6-GGCC	0.000	0.706

	Mean	Stdev
slide-6-GGCC	-0.910	0.782

	Mean	Stdev
tilt-6-GGCC	0.000	5.000

	Mean	Stdev
twist-6-GGCC	31.421	5.618

	Mean	Stdev
rise-7-GATC	3.272	0.322

	Mean	Stdev
roll-7-GATC	3.270	6.134

	Mean	Stdev
shift-7-GATC	0.000	0.728

	Mean	Stdev
slide-7-GATC	-0.295	0.718

	Mean	Stdev
tilt-7-GATC	0.000	4.582

	Mean	Stdev
twist-7-GATC	33.732	6.568

	Mean	Stdev
rise-8-AATT	3.191	0.296

	Mean	Stdev
roll-8-AATT	1.602	5.804

	Mean	Stdev
shift-8-AATT	0.000	0.652

	Mean	Stdev
slide-8-AATT	-0.473	0.601

	Mean	Stdev
tilt-8-AATT	0.000	4.813

	Mean	Stdev
twist-8-AATT	32.160	5.664

	Mean	Stdev
rise-9-AGCT	3.336	0.319

	Mean	Stdev
roll-9-AGCT	3.852	5.746

	Mean	Stdev
shift-9-AGCT	0.000	0.716

	Mean	Stdev
slide-9-AGCT	-0.782	0.624

	Mean	Stdev
tilt-9-AGCT	0.000	4.612

	Mean	Stdev
twist-9-AGCT	32.125	5.267

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