NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	G	-	C	-	A	-	G	-	G	-	C	-	A	-	G	-	G	-	C	-	A	-	G	-	G	-	C	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	C	-	G	-	T	-	C	-	C	-	G	-	T	-	C	-	C	-	G	-	T	-	C	-	C	-	G	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.013	0.000	0.000	-0.093	0.000	0.000
Slide	0.000	2.386	1.556	0.000	-0.034	-0.142
Rise	0.000	1.556	8.878	0.000	0.006	-0.184
Tilt	-0.093	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.034	0.006	0.000	0.018	0.007
Twist	0.000	-0.142	-0.184	0.000	0.007	0.026

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.049	0.000	0.000	-0.089	0.000	0.000
Slide	0.000	2.272	1.492	0.000	-0.031	-0.134
Rise	0.000	1.492	7.951	0.000	0.015	-0.168
Tilt	-0.089	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.031	0.015	0.000	0.019	0.006
Twist	0.000	-0.134	-0.168	0.000	0.006	0.027

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.046	0.000	0.000	-0.101	0.000	0.000
Slide	0.000	2.442	1.521	0.000	-0.026	-0.155
Rise	0.000	1.521	8.530	0.000	0.019	-0.185
Tilt	-0.101	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.026	0.019	0.000	0.020	0.008
Twist	0.000	-0.155	-0.185	0.000	0.008	0.032

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.228	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	2.524	0.542	0.000	0.012	-0.051
Rise	0.000	0.542	8.360	0.000	-0.166	-0.257
Tilt	-0.026	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.012	-0.166	0.000	0.024	0.011
Twist	0.000	-0.051	-0.257	0.000	0.011	0.019

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.186	0.000	0.000	-0.027	0.000	0.000
Slide	0.000	2.300	0.424	0.000	0.017	-0.054
Rise	0.000	0.424	7.914	0.000	-0.153	-0.245
Tilt	-0.027	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.017	-0.153	0.000	0.023	0.011
Twist	0.000	-0.054	-0.245	0.000	0.011	0.019

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.250	0.000	0.000	-0.022	0.000	0.000
Slide	0.000	2.673	0.524	0.000	0.015	-0.045
Rise	0.000	0.524	8.654	0.000	-0.169	-0.258
Tilt	-0.022	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.015	-0.169	0.000	0.025	0.010
Twist	0.000	-0.045	-0.258	0.000	0.010	0.019

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.218	0.000	0.000	-0.099	0.000	0.000
Slide	0.000	2.124	0.602	0.000	0.019	-0.050
Rise	0.000	0.602	6.943	0.000	-0.074	-0.219
Tilt	-0.099	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.019	-0.074	0.000	0.018	0.005
Twist	0.000	-0.050	-0.219	0.000	0.005	0.016

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.399	0.000	0.000	-0.125	0.000	0.000
Slide	0.000	2.216	1.201	0.000	0.004	-0.067
Rise	0.000	1.201	6.573	0.000	-0.078	-0.219
Tilt	-0.125	0.000	0.000	0.035	0.000	0.000
Roll	0.000	0.004	-0.078	0.000	0.018	0.007
Twist	0.000	-0.067	-0.219	0.000	0.007	0.019

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.370	0.000	0.000	-0.093	0.000	0.000
Slide	0.000	3.443	2.787	0.000	0.061	-0.156
Rise	0.000	2.787	11.421	0.000	0.140	-0.203
Tilt	-0.093	0.000	0.000	0.045	0.000	0.000
Roll	0.000	0.061	0.140	0.000	0.025	0.006
Twist	0.000	-0.156	-0.203	0.000	0.006	0.039

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.531	0.000	0.000	-0.120	0.000	0.000
Slide	0.000	2.791	2.261	0.000	0.065	-0.120
Rise	0.000	2.261	10.419	0.000	0.137	-0.207
Tilt	-0.120	0.000	0.000	0.044	0.000	0.000
Roll	0.000	0.065	0.137	0.000	0.025	0.007
Twist	0.000	-0.120	-0.207	0.000	0.007	0.035

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.217	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	3.217	2.288	0.000	0.073	-0.155
Rise	0.000	2.288	10.547	0.000	0.144	-0.248
Tilt	-0.106	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.073	0.144	0.000	0.022	0.005
Twist	0.000	-0.155	-0.248	0.000	0.005	0.033

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.365	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	3.619	2.882	0.000	0.065	-0.173
Rise	0.000	2.882	11.676	0.000	0.163	-0.227
Tilt	-0.077	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.065	0.163	0.000	0.025	0.006
Twist	0.000	-0.173	-0.227	0.000	0.006	0.041

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.821	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	2.290	2.024	0.000	-0.029	-0.162
Rise	0.000	2.024	7.812	0.000	-0.051	-0.183
Tilt	-0.094	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.029	-0.051	0.000	0.026	0.008
Twist	0.000	-0.162	-0.183	0.000	0.008	0.035

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.559	0.000	0.000	-0.085	0.000	0.000
Slide	0.000	1.963	1.924	0.000	-0.017	-0.121
Rise	0.000	1.924	7.736	0.000	-0.036	-0.176
Tilt	-0.085	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.017	-0.036	0.000	0.023	0.007
Twist	0.000	-0.121	-0.176	0.000	0.007	0.030

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.810	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.347	1.866	0.000	-0.029	-0.158
Rise	0.000	1.866	7.173	0.000	-0.040	-0.176
Tilt	-0.088	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.029	-0.040	0.000	0.025	0.008
Twist	0.000	-0.158	-0.176	0.000	0.008	0.034

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-agct.13.cte	0.000	0.000

	Mean	Stdev
-agct.5.cte	0.000	0.000

	Mean	Stdev
-agct.9.cte	0.000	0.000

	Mean	Stdev
-catg.12.cte	0.000	0.000

	Mean	Stdev
-catg.4.cte	0.000	0.000

	Mean	Stdev
-catg.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.16.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-gcgc.11.cte	0.000	0.000

	Mean	Stdev
-gcgc.15.cte	0.000	0.000

	Mean	Stdev
-gcgc.3.cte	0.000	0.000

	Mean	Stdev
-gcgc.7.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.647	0.314

	Mean	Stdev
roll-10-GGCC	5.345	4.919

	Mean	Stdev
shift-10-GGCC	0.000	0.606

	Mean	Stdev
slide-10-GGCC	-1.018	0.661

	Mean	Stdev
tilt-10-GGCC	0.000	4.112

	Mean	Stdev
twist-10-GGCC	34.677	5.119

	Mean	Stdev
rise-11-GCGC	3.365	0.264

	Mean	Stdev
roll-11-GCGC	-0.641	5.449

	Mean	Stdev
shift-11-GCGC	0.000	0.706

	Mean	Stdev
slide-11-GCGC	-0.346	0.504

	Mean	Stdev
tilt-11-GCGC	0.000	3.908

	Mean	Stdev
twist-11-GCGC	35.485	4.645

	Mean	Stdev
rise-12-CATG	2.913	0.349

	Mean	Stdev
roll-12-CATG	9.509	5.785

	Mean	Stdev
shift-12-CATG	0.000	0.698

	Mean	Stdev
slide-12-CATG	-0.150	0.506

	Mean	Stdev
tilt-12-CATG	0.000	4.583

	Mean	Stdev
twist-12-CATG	24.688	8.174

	Mean	Stdev
rise-13-AGCT	3.299	0.285

	Mean	Stdev
roll-13-AGCT	1.163	6.056

	Mean	Stdev
shift-13-AGCT	0.000	0.898

	Mean	Stdev
slide-13-AGCT	-0.315	0.610

	Mean	Stdev
tilt-13-AGCT	0.000	5.214

	Mean	Stdev
twist-13-AGCT	34.145	6.219

	Mean	Stdev
rise-14-GGCC	3.611	0.321

	Mean	Stdev
roll-14-GGCC	5.010	5.226

	Mean	Stdev
shift-14-GGCC	0.000	0.656

	Mean	Stdev
slide-14-GGCC	-0.891	0.684

	Mean	Stdev
tilt-14-GGCC	0.000	4.262

	Mean	Stdev
twist-14-GGCC	34.870	5.307

	Mean	Stdev
rise-15-GCGC	3.396	0.280

	Mean	Stdev
roll-15-GCGC	-0.577	5.627

	Mean	Stdev
shift-15-GCGC	0.000	0.697

	Mean	Stdev
slide-15-GCGC	-0.401	0.548

	Mean	Stdev
tilt-15-GCGC	0.000	4.095

	Mean	Stdev
twist-15-GCGC	34.932	4.971

	Mean	Stdev
rise-16-CGCG	3.126	0.387

	Mean	Stdev
roll-16-CGCG	8.296	6.204

	Mean	Stdev
shift-16-CGCG	0.000	0.819

	Mean	Stdev
slide-16-CGCG	-0.038	0.558

	Mean	Stdev
tilt-16-CGCG	0.000	5.358

	Mean	Stdev
twist-16-CGCG	30.560	8.667

	Mean	Stdev
rise-2-CGCG	3.311	0.382

	Mean	Stdev
roll-2-CGCG	8.436	6.262

	Mean	Stdev
shift-2-CGCG	0.000	0.788

	Mean	Stdev
slide-2-CGCG	-0.106	0.558

	Mean	Stdev
tilt-2-CGCG	0.000	5.006

	Mean	Stdev
twist-2-CGCG	34.798	7.428

	Mean	Stdev
rise-3-GCGC	3.444	0.289

	Mean	Stdev
roll-3-GCGC	-2.805	6.319

	Mean	Stdev
shift-3-GCGC	0.000	0.784

	Mean	Stdev
slide-3-GCGC	-0.194	0.538

	Mean	Stdev
tilt-3-GCGC	0.000	4.163

	Mean	Stdev
twist-3-GCGC	35.430	5.680

	Mean	Stdev
rise-4-CATG	2.966	0.351

	Mean	Stdev
roll-4-CATG	9.726	6.136

	Mean	Stdev
shift-4-CATG	0.000	0.711

	Mean	Stdev
slide-4-CATG	-0.151	0.543

	Mean	Stdev
tilt-4-CATG	0.000	4.606

	Mean	Stdev
twist-4-CATG	26.037	8.331

	Mean	Stdev
rise-5-AGCT	3.353	0.301

	Mean	Stdev
roll-5-AGCT	1.876	5.894

	Mean	Stdev
shift-5-AGCT	0.000	0.866

	Mean	Stdev
slide-5-AGCT	-0.378	0.616

	Mean	Stdev
tilt-5-AGCT	0.000	5.160

	Mean	Stdev
twist-5-AGCT	34.374	5.881

	Mean	Stdev
rise-6-GGCC	3.659	0.324

	Mean	Stdev
roll-6-GGCC	5.228	5.077

	Mean	Stdev
shift-6-GGCC	0.000	0.605

	Mean	Stdev
slide-6-GGCC	-1.059	0.638

	Mean	Stdev
tilt-6-GGCC	0.000	4.203

	Mean	Stdev
twist-6-GGCC	34.642	5.186

	Mean	Stdev
rise-7-GCGC	3.366	0.266

	Mean	Stdev
roll-7-GCGC	-0.800	5.435

	Mean	Stdev
shift-7-GCGC	0.000	0.692

	Mean	Stdev
slide-7-GCGC	-0.370	0.493

	Mean	Stdev
tilt-7-GCGC	0.000	3.931

	Mean	Stdev
twist-7-GCGC	35.494	4.573

	Mean	Stdev
rise-8-CATG	2.884	0.341

	Mean	Stdev
roll-8-CATG	9.661	5.671

	Mean	Stdev
shift-8-CATG	0.000	0.690

	Mean	Stdev
slide-8-CATG	-0.151	0.486

	Mean	Stdev
tilt-8-CATG	0.000	4.544

	Mean	Stdev
twist-8-CATG	23.773	7.990

	Mean	Stdev
rise-9-AGCT	3.322	0.289

	Mean	Stdev
roll-9-AGCT	0.884	5.842

	Mean	Stdev
shift-9-AGCT	0.000	0.905

	Mean	Stdev
slide-9-AGCT	-0.284	0.597

	Mean	Stdev
tilt-9-AGCT	0.000	5.285

	Mean	Stdev
twist-9-AGCT	35.090	5.519

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	G	-	C	-	A	-	G	-	G	-	C	-	A	-	G	-	G	-	C	-	A	-	G	-	G	-	C	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	C	-	G	-	T	-	C	-	C	-	G	-	T	-	C	-	C	-	G	-	T	-	C	-	C	-	G	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	G	-	C	-	A	-	G	-	G	-	C	-	A	-	G	-	G	-	C	-	A	-	G	-	G	-	C	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	C	-	G	-	T	-	C	-	C	-	G	-	T	-	C	-	C	-	G	-	T	-	C	-	C	-	G	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	G	-	C	-	A	-	G	-	G	-	C	-	A	-	G	-	G	-	C	-	A	-	G	-	G	-	C	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	C	-	G	-	T	-	C	-	C	-	G	-	T	-	C	-	C	-	G	-	T	-	C	-	C	-	G	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19