NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.193	0.000	0.000	-0.110	0.000	0.000
Slide	0.000	3.790	0.922	0.000	-0.053	-0.235
Rise	0.000	0.922	9.463	0.000	0.010	-0.195
Tilt	-0.110	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.053	0.010	0.000	0.021	0.011
Twist	0.000	-0.235	-0.195	0.000	0.011	0.049

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.175	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	3.095	0.585	0.000	-0.058	-0.180
Rise	0.000	0.585	9.747	0.000	0.016	-0.172
Tilt	-0.095	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.058	0.016	0.000	0.021	0.011
Twist	0.000	-0.180	-0.172	0.000	0.011	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.250	0.000	0.000	-0.104	0.000	0.000
Slide	0.000	3.424	0.906	0.000	-0.056	-0.230
Rise	0.000	0.906	9.330	0.000	0.013	-0.191
Tilt	-0.104	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.056	0.013	0.000	0.021	0.010
Twist	0.000	-0.230	-0.191	0.000	0.010	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.941	0.000	0.000	-0.118	0.000	0.000
Slide	0.000	3.493	1.809	0.000	-0.090	-0.158
Rise	0.000	1.809	7.507	0.000	-0.062	-0.148
Tilt	-0.118	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.090	-0.062	0.000	0.026	0.005
Twist	0.000	-0.158	-0.148	0.000	0.005	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.618	0.000	0.000	-0.049	0.000	0.000
Slide	0.000	2.852	1.655	0.000	-0.066	-0.154
Rise	0.000	1.655	6.702	0.000	-0.050	-0.148
Tilt	-0.049	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.066	-0.050	0.000	0.025	0.007
Twist	0.000	-0.154	-0.148	0.000	0.007	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.210	0.000	0.000	-0.123	0.000	0.000
Slide	0.000	2.127	1.430	0.000	-0.049	-0.107
Rise	0.000	1.430	7.133	0.000	-0.034	-0.191
Tilt	-0.123	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.049	-0.034	0.000	0.020	0.006
Twist	0.000	-0.107	-0.191	0.000	0.006	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.954	0.000	0.000	-0.122	0.000	0.000
Slide	0.000	3.342	1.812	0.000	-0.079	-0.157
Rise	0.000	1.812	7.334	0.000	-0.059	-0.149
Tilt	-0.122	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.079	-0.059	0.000	0.025	0.005
Twist	0.000	-0.157	-0.149	0.000	0.005	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.613	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	2.682	0.582	0.000	0.020	-0.036
Rise	0.000	0.582	8.650	0.000	-0.183	-0.276
Tilt	-0.002	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.020	-0.183	0.000	0.026	0.012
Twist	0.000	-0.036	-0.276	0.000	0.012	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.122	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	1.714	0.912	0.000	0.007	-0.063
Rise	0.000	0.912	5.870	0.000	-0.065	-0.175
Tilt	-0.059	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.007	-0.065	0.000	0.020	0.008
Twist	0.000	-0.063	-0.175	0.000	0.008	0.018

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.614	0.000	0.000	-0.011	0.000	0.000
Slide	0.000	2.249	0.844	0.000	0.009	-0.026
Rise	0.000	0.844	7.914	0.000	-0.156	-0.254
Tilt	-0.011	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.009	-0.156	0.000	0.027	0.011
Twist	0.000	-0.026	-0.254	0.000	0.011	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.721	0.000	0.000	-0.001	0.000	0.000
Slide	0.000	2.722	0.282	0.000	0.021	-0.022
Rise	0.000	0.282	8.883	0.000	-0.186	-0.279
Tilt	-0.001	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.021	-0.186	0.000	0.027	0.012
Twist	0.000	-0.022	-0.279	0.000	0.012	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.303	0.000	0.000	-0.119	0.000	0.000
Slide	0.000	3.489	2.503	0.000	0.069	-0.178
Rise	0.000	2.503	11.709	0.000	0.148	-0.238
Tilt	-0.119	0.000	0.000	0.046	0.000	0.000
Roll	0.000	0.069	0.148	0.000	0.025	0.003
Twist	0.000	-0.178	-0.238	0.000	0.003	0.045

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.401	0.000	0.000	-0.123	0.000	0.000
Slide	0.000	3.417	2.625	0.000	0.080	-0.186
Rise	0.000	2.625	11.343	0.000	0.155	-0.240
Tilt	-0.123	0.000	0.000	0.045	0.000	0.000
Roll	0.000	0.080	0.155	0.000	0.024	0.004
Twist	0.000	-0.186	-0.240	0.000	0.004	0.045

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.281	0.000	0.000	-0.109	0.000	0.000
Slide	0.000	3.743	2.564	0.000	0.077	-0.198
Rise	0.000	2.564	11.736	0.000	0.171	-0.218
Tilt	-0.109	0.000	0.000	0.046	0.000	0.000
Roll	0.000	0.077	0.171	0.000	0.025	0.003
Twist	0.000	-0.198	-0.218	0.000	0.003	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.853	0.000	0.000	-0.089	0.000	0.000
Slide	0.000	2.207	1.674	0.000	-0.014	-0.155
Rise	0.000	1.674	7.123	0.000	-0.039	-0.158
Tilt	-0.089	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.014	-0.039	0.000	0.022	0.006
Twist	0.000	-0.155	-0.158	0.000	0.006	0.037

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-agct.11.cte	0.000	0.000

	Mean	Stdev
-agct.15.cte	0.000	0.000

	Mean	Stdev
-agct.3.cte	0.000	0.000

	Mean	Stdev
-agct.7.cte	0.000	0.000

	Mean	Stdev
-catg.13.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-catg.5.cte	0.000	0.000

	Mean	Stdev
-catg.9.cte	0.000	0.000

	Mean	Stdev
-gcgc.12.cte	0.000	0.000

	Mean	Stdev
-gcgc.4.cte	0.000	0.000

	Mean	Stdev
-gcgc.8.cte	0.000	0.000

	Mean	Stdev
-ggcc.16.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.329	0.262

	Mean	Stdev
roll-10-AATT	-1.183	5.623

	Mean	Stdev
shift-10-AATT	0.000	0.850

	Mean	Stdev
slide-10-AATT	0.083	0.469

	Mean	Stdev
tilt-10-AATT	0.000	5.212

	Mean	Stdev
twist-10-AATT	37.820	4.477

	Mean	Stdev
rise-11-AGCT	3.551	0.302

	Mean	Stdev
roll-11-AGCT	4.705	4.952

	Mean	Stdev
shift-11-AGCT	0.000	0.623

	Mean	Stdev
slide-11-AGCT	-0.761	0.486

	Mean	Stdev
tilt-11-AGCT	0.000	4.836

	Mean	Stdev
twist-11-AGCT	35.354	4.400

	Mean	Stdev
rise-12-GCGC	3.424	0.256

	Mean	Stdev
roll-12-GCGC	-1.476	5.258

	Mean	Stdev
shift-12-GCGC	0.000	0.766

	Mean	Stdev
slide-12-GCGC	-0.357	0.495

	Mean	Stdev
tilt-12-GCGC	0.000	4.067

	Mean	Stdev
twist-12-GCGC	36.108	4.325

	Mean	Stdev
rise-13-CATG	2.919	0.340

	Mean	Stdev
roll-13-CATG	11.342	5.574

	Mean	Stdev
shift-13-CATG	0.000	0.603

	Mean	Stdev
slide-13-CATG	-0.178	0.481

	Mean	Stdev
tilt-13-CATG	0.000	4.646

	Mean	Stdev
twist-13-CATG	22.263	7.364

	Mean	Stdev
rise-14-AATT	3.249	0.260

	Mean	Stdev
roll-14-AATT	-0.664	5.838

	Mean	Stdev
shift-14-AATT	0.000	0.834

	Mean	Stdev
slide-14-AATT	-0.012	0.516

	Mean	Stdev
tilt-14-AATT	0.000	5.497

	Mean	Stdev
twist-14-AATT	36.924	5.299

	Mean	Stdev
rise-15-AGCT	3.514	0.323

	Mean	Stdev
roll-15-AGCT	3.520	5.056

	Mean	Stdev
shift-15-AGCT	0.000	0.616

	Mean	Stdev
slide-15-AGCT	-0.812	0.541

	Mean	Stdev
tilt-15-AGCT	0.000	4.350

	Mean	Stdev
twist-15-AGCT	33.595	4.455

	Mean	Stdev
rise-16-GGCC	3.608	0.319

	Mean	Stdev
roll-16-GGCC	4.810	5.312

	Mean	Stdev
shift-16-GGCC	0.000	0.601

	Mean	Stdev
slide-16-GGCC	-1.069	0.650

	Mean	Stdev
tilt-16-GGCC	0.000	4.424

	Mean	Stdev
twist-16-GGCC	34.420	4.890

	Mean	Stdev
rise-2-CATG	3.247	0.378

	Mean	Stdev
roll-2-CATG	8.744	6.202

	Mean	Stdev
shift-2-CATG	0.000	0.767

	Mean	Stdev
slide-2-CATG	-0.152	0.648

	Mean	Stdev
tilt-2-CATG	0.000	4.914

	Mean	Stdev
twist-2-CATG	32.177	7.794

	Mean	Stdev
rise-3-AGCT	3.404	0.329

	Mean	Stdev
roll-3-AGCT	4.530	5.756

	Mean	Stdev
shift-3-AGCT	0.000	0.912

	Mean	Stdev
slide-3-AGCT	-0.433	0.610

	Mean	Stdev
tilt-3-AGCT	0.000	5.790

	Mean	Stdev
twist-3-AGCT	34.548	5.940

	Mean	Stdev
rise-4-GCGC	3.432	0.264

	Mean	Stdev
roll-4-GCGC	-1.454	5.634

	Mean	Stdev
shift-4-GCGC	0.000	0.742

	Mean	Stdev
slide-4-GCGC	-0.355	0.520

	Mean	Stdev
tilt-4-GCGC	0.000	4.147

	Mean	Stdev
twist-4-GCGC	36.122	4.397

	Mean	Stdev
rise-5-CATG	2.997	0.354

	Mean	Stdev
roll-5-CATG	11.871	5.368

	Mean	Stdev
shift-5-CATG	0.000	0.604

	Mean	Stdev
slide-5-CATG	-0.205	0.525

	Mean	Stdev
tilt-5-CATG	0.000	4.792

	Mean	Stdev
twist-5-CATG	23.042	7.241

	Mean	Stdev
rise-6-AATT	3.323	0.266

	Mean	Stdev
roll-6-AATT	-0.604	5.596

	Mean	Stdev
shift-6-AATT	0.000	0.809

	Mean	Stdev
slide-6-AATT	0.013	0.521

	Mean	Stdev
tilt-6-AATT	0.000	5.204

	Mean	Stdev
twist-6-AATT	37.092	5.094

	Mean	Stdev
rise-7-AGCT	3.542	0.307

	Mean	Stdev
roll-7-AGCT	4.475	5.086

	Mean	Stdev
shift-7-AGCT	0.000	0.628

	Mean	Stdev
slide-7-AGCT	-0.782	0.499

	Mean	Stdev
tilt-7-AGCT	0.000	4.927

	Mean	Stdev
twist-7-AGCT	35.400	4.474

	Mean	Stdev
rise-8-GCGC	3.420	0.256

	Mean	Stdev
roll-8-GCGC	-1.556	5.413

	Mean	Stdev
shift-8-GCGC	0.000	0.757

	Mean	Stdev
slide-8-GCGC	-0.327	0.487

	Mean	Stdev
tilt-8-GCGC	0.000	3.988

	Mean	Stdev
twist-8-GCGC	36.266	4.267

	Mean	Stdev
rise-9-CATG	2.890	0.326

	Mean	Stdev
roll-9-CATG	11.424	5.363

	Mean	Stdev
shift-9-CATG	0.000	0.584

	Mean	Stdev
slide-9-CATG	-0.156	0.470

	Mean	Stdev
tilt-9-CATG	0.000	4.761

	Mean	Stdev
twist-9-CATG	21.270	6.704

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