NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.839	0.000	0.000	-0.006	0.000	0.000
Slide	0.000	3.466	2.562	0.000	0.031	-0.112
Rise	0.000	2.562	9.762	0.000	0.072	-0.231
Tilt	-0.006	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.031	0.072	0.000	0.023	0.011
Twist	0.000	-0.112	-0.231	0.000	0.011	0.038

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.802	0.000	0.000	-0.034	0.000	0.000
Slide	0.000	3.890	2.761	0.000	0.039	-0.103
Rise	0.000	2.761	9.960	0.000	0.084	-0.208
Tilt	-0.034	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.039	0.084	0.000	0.025	0.011
Twist	0.000	-0.103	-0.208	0.000	0.011	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.876	0.000	0.000	-0.012	0.000	0.000
Slide	0.000	3.343	2.661	0.000	0.019	-0.089
Rise	0.000	2.661	9.759	0.000	0.067	-0.235
Tilt	-0.012	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.019	0.067	0.000	0.023	0.011
Twist	0.000	-0.089	-0.235	0.000	0.011	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.400	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	1.804	1.138	0.000	-0.064	-0.016
Rise	0.000	1.138	6.769	0.000	-0.044	-0.100
Tilt	-0.084	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.064	-0.044	0.000	0.022	0.004
Twist	0.000	-0.016	-0.100	0.000	0.004	0.015

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.359	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.373	0.803	0.000	0.002	-0.069
Rise	0.000	0.803	7.304	0.000	-0.105	-0.253
Tilt	-0.088	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.002	-0.105	0.000	0.020	0.007
Twist	0.000	-0.069	-0.253	0.000	0.007	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.885	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	1.118	-0.372	0.000	0.038	0.044
Rise	0.000	-0.370	5.033	0.000	0.012	-0.023
Tilt	-0.026	0.000	0.000	0.015	0.000	0.000
Roll	0.000	0.038	0.012	0.000	0.017	-0.000
Twist	0.000	0.044	-0.023	0.000	-0.000	0.003

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.329	0.000	0.000	-0.104	0.000	0.000
Slide	0.000	2.116	1.090	0.000	0.006	-0.073
Rise	0.000	1.090	6.977	0.000	-0.087	-0.251
Tilt	-0.104	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.006	-0.087	0.000	0.019	0.007
Twist	0.000	-0.073	-0.251	0.000	0.007	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.309	0.000	0.000	-0.100	0.000	0.000
Slide	0.000	2.187	0.894	0.000	0.005	-0.068
Rise	0.000	0.894	6.773	0.000	-0.080	-0.237
Tilt	-0.100	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.005	-0.080	0.000	0.017	0.006
Twist	0.000	-0.068	-0.237	0.000	0.006	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.231	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	2.126	1.553	0.000	-0.037	-0.171
Rise	0.000	1.553	7.768	0.000	-0.034	-0.189
Tilt	-0.084	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.037	-0.034	0.000	0.023	0.012
Twist	0.000	-0.171	-0.189	0.000	0.012	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.271	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	1.873	1.346	0.000	-0.033	-0.125
Rise	0.000	1.346	7.223	0.000	-0.057	-0.172
Tilt	-0.055	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.033	-0.057	0.000	0.022	0.013
Twist	0.000	-0.125	-0.172	0.000	0.013	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.107	0.000	0.000	-0.145	0.000	0.000
Slide	0.000	0.887	0.945	0.000	0.003	0.005
Rise	0.000	0.945	7.829	0.000	-0.031	-0.165
Tilt	-0.145	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.003	-0.031	0.000	0.025	0.008
Twist	0.000	0.005	-0.165	0.000	0.008	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.179	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.136	1.467	0.000	-0.045	-0.166
Rise	0.000	1.467	7.721	0.000	-0.033	-0.182
Tilt	-0.077	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.045	-0.033	0.000	0.022	0.012
Twist	0.000	-0.166	-0.182	0.000	0.012	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.991	0.000	0.000	-0.078	0.000	0.000
Slide	0.000	1.342	1.500	0.000	-0.015	-0.069
Rise	0.000	1.500	7.181	0.000	-0.035	-0.194
Tilt	-0.078	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.015	-0.035	0.000	0.022	0.010
Twist	0.000	-0.069	-0.194	0.000	0.010	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.921	0.000	0.000	-0.079	0.000	0.000
Slide	0.000	1.526	1.600	0.000	-0.014	-0.082
Rise	0.000	1.600	7.782	0.000	-0.030	-0.208
Tilt	-0.079	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.014	-0.030	0.000	0.021	0.009
Twist	0.000	-0.082	-0.208	0.000	0.009	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.063	0.000	0.000	-0.083	0.000	0.000
Slide	0.000	1.335	1.624	0.000	-0.018	-0.068
Rise	0.000	1.624	7.181	0.000	-0.047	-0.199
Tilt	-0.083	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.018	-0.047	0.000	0.021	0.009
Twist	0.000	-0.068	-0.199	0.000	0.009	0.020

[Image courtesy of Curves+]

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	Mean	Stdev
-acgt.12.cte	0.000	0.000

	Mean	Stdev
-acgt.4.cte	0.000	0.000

	Mean	Stdev
-acgt.8.cte	0.000	0.000

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-cgcg.13.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.5.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-gatc.11.cte	0.000	0.000

	Mean	Stdev
-gatc.15.cte	0.000	0.000

	Mean	Stdev
-gatc.3.cte	0.000	0.000

	Mean	Stdev
-gatc.7.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.476	0.359

	Mean	Stdev
roll-10-GGCC	5.612	5.989

	Mean	Stdev
shift-10-GGCC	0.000	0.844

	Mean	Stdev
slide-10-GGCC	-0.394	0.778

	Mean	Stdev
tilt-10-GGCC	0.000	4.415

	Mean	Stdev
twist-10-GGCC	31.854	7.456

	Mean	Stdev
rise-11-GATC	3.357	0.301

	Mean	Stdev
roll-11-GATC	1.540	5.576

	Mean	Stdev
shift-11-GATC	0.000	0.769

	Mean	Stdev
slide-11-GATC	-0.184	0.677

	Mean	Stdev
tilt-11-GATC	0.000	4.938

	Mean	Stdev
twist-11-GATC	37.570	5.386

	Mean	Stdev
rise-12-ACGT	3.364	0.297

	Mean	Stdev
roll-12-ACGT	-0.674	5.928

	Mean	Stdev
shift-12-ACGT	0.000	0.836

	Mean	Stdev
slide-12-ACGT	-0.589	0.470

	Mean	Stdev
tilt-12-ACGT	0.000	3.855

	Mean	Stdev
twist-12-ACGT	31.475	4.991

	Mean	Stdev
rise-13-CGCG	3.023	0.427

	Mean	Stdev
roll-13-CGCG	9.039	5.967

	Mean	Stdev
shift-13-CGCG	0.000	0.736

	Mean	Stdev
slide-13-CGCG	-0.045	0.544

	Mean	Stdev
tilt-13-CGCG	0.000	5.162

	Mean	Stdev
twist-13-CGCG	28.690	10.180

	Mean	Stdev
rise-14-GGCC	3.458	0.340

	Mean	Stdev
roll-14-GGCC	4.909	6.044

	Mean	Stdev
shift-14-GGCC	0.000	0.883

	Mean	Stdev
slide-14-GGCC	-0.285	0.737

	Mean	Stdev
tilt-14-GGCC	0.000	4.422

	Mean	Stdev
twist-14-GGCC	33.363	7.407

	Mean	Stdev
rise-15-GATC	3.367	0.314

	Mean	Stdev
roll-15-GATC	2.013	5.912

	Mean	Stdev
shift-15-GATC	0.000	0.708

	Mean	Stdev
slide-15-GATC	-0.268	0.676

	Mean	Stdev
tilt-15-GATC	0.000	4.611

	Mean	Stdev
twist-15-GATC	35.561	5.882

	Mean	Stdev
rise-16-AGCT	3.415	0.327

	Mean	Stdev
roll-16-AGCT	3.561	5.540

	Mean	Stdev
shift-16-AGCT	0.000	0.709

	Mean	Stdev
slide-16-AGCT	-0.694	0.634

	Mean	Stdev
tilt-16-AGCT	0.000	4.840

	Mean	Stdev
twist-16-AGCT	33.073	6.828

	Mean	Stdev
rise-2-CGCG	3.244	0.348

	Mean	Stdev
roll-2-CGCG	5.388	7.030

	Mean	Stdev
shift-2-CGCG	0.000	0.837

	Mean	Stdev
slide-2-CGCG	1.342	1.399

	Mean	Stdev
tilt-2-CGCG	0.000	6.476

	Mean	Stdev
twist-2-CGCG	7.688	26.965

	Mean	Stdev
rise-3-GATC	3.217	0.328

	Mean	Stdev
roll-3-GATC	1.430	5.241

	Mean	Stdev
shift-3-GATC	0.000	1.244

	Mean	Stdev
slide-3-GATC	0.855	0.891

	Mean	Stdev
tilt-3-GATC	0.000	7.720

	Mean	Stdev
twist-3-GATC	35.090	6.169

	Mean	Stdev
rise-4-ACGT	3.385	0.291

	Mean	Stdev
roll-4-ACGT	-0.822	5.654

	Mean	Stdev
shift-4-ACGT	0.000	0.870

	Mean	Stdev
slide-4-ACGT	-0.575	0.442

	Mean	Stdev
tilt-4-ACGT	0.000	3.905

	Mean	Stdev
twist-4-ACGT	31.150	4.877

	Mean	Stdev
rise-5-CGCG	3.188	0.422

	Mean	Stdev
roll-5-CGCG	9.064	6.272

	Mean	Stdev
shift-5-CGCG	0.000	0.789

	Mean	Stdev
slide-5-CGCG	-0.032	0.584

	Mean	Stdev
tilt-5-CGCG	0.000	5.455

	Mean	Stdev
twist-5-CGCG	32.330	9.521

	Mean	Stdev
rise-6-GGCC	3.486	0.369

	Mean	Stdev
roll-6-GGCC	6.118	5.912

	Mean	Stdev
shift-6-GGCC	0.000	0.820

	Mean	Stdev
slide-6-GGCC	-0.460	0.794

	Mean	Stdev
tilt-6-GGCC	0.000	4.477

	Mean	Stdev
twist-6-GGCC	31.344	7.351

	Mean	Stdev
rise-7-GATC	3.332	0.300

	Mean	Stdev
roll-7-GATC	1.648	5.725

	Mean	Stdev
shift-7-GATC	0.000	0.774

	Mean	Stdev
slide-7-GATC	-0.168	0.663

	Mean	Stdev
tilt-7-GATC	0.000	4.925

	Mean	Stdev
twist-7-GATC	37.358	5.392

	Mean	Stdev
rise-8-ACGT	3.333	0.309

	Mean	Stdev
roll-8-ACGT	-0.072	5.974

	Mean	Stdev
shift-8-ACGT	0.000	0.820

	Mean	Stdev
slide-8-ACGT	-0.597	0.476

	Mean	Stdev
tilt-8-ACGT	0.000	3.910

	Mean	Stdev
twist-8-ACGT	30.783	5.215

	Mean	Stdev
rise-9-CGCG	3.130	0.429

	Mean	Stdev
roll-9-CGCG	8.772	6.344

	Mean	Stdev
shift-9-CGCG	0.000	0.784

	Mean	Stdev
slide-9-CGCG	-0.030	0.567

	Mean	Stdev
tilt-9-CGCG	0.000	5.367

	Mean	Stdev
twist-9-CGCG	31.336	9.856

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