NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.939	0.000	0.000	-0.100	0.000	0.000
Slide	0.000	2.246	1.742	0.000	-0.015	-0.172
Rise	0.000	1.742	8.681	0.000	-0.018	-0.197
Tilt	-0.100	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.015	-0.018	0.000	0.023	0.009
Twist	0.000	-0.172	-0.197	0.000	0.009	0.036

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.962	0.000	0.000	-0.114	0.000	0.000
Slide	0.000	1.972	1.419	0.000	-0.011	-0.140
Rise	0.000	1.419	8.450	0.000	-0.008	-0.176
Tilt	-0.114	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.011	-0.008	0.000	0.022	0.010
Twist	0.000	-0.140	-0.176	0.000	0.010	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.943	0.000	0.000	-0.101	0.000	0.000
Slide	0.000	2.294	1.600	0.000	-0.014	-0.181
Rise	0.000	1.600	8.480	0.000	-0.014	-0.173
Tilt	-0.101	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.014	-0.014	0.000	0.022	0.007
Twist	0.000	-0.181	-0.173	0.000	0.007	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.514	0.000	0.000	-0.066	0.000	0.000
Slide	0.000	2.823	-0.114	0.000	0.018	-0.064
Rise	0.000	-0.114	9.296	0.000	-0.154	-0.276
Tilt	-0.066	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.018	-0.154	0.000	0.025	0.008
Twist	0.000	-0.064	-0.276	0.000	0.008	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.367	0.000	0.000	0.005	0.000	0.000
Slide	0.000	0.856	-0.487	0.000	-0.001	0.021
Rise	0.000	-0.487	3.240	0.000	-0.070	-0.086
Tilt	0.005	0.000	0.000	0.012	0.000	0.000
Roll	0.000	-0.001	-0.070	0.000	0.007	0.001
Twist	0.000	0.021	-0.086	0.000	0.001	0.006

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.441	0.000	0.000	-0.137	0.000	0.000
Slide	0.000	2.341	1.108	0.000	0.006	-0.077
Rise	0.000	1.108	6.961	0.000	-0.076	-0.233
Tilt	-0.137	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.006	-0.076	0.000	0.018	0.007
Twist	0.000	-0.077	-0.233	0.000	0.007	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.464	0.000	0.000	-0.060	0.000	0.000
Slide	0.000	2.704	0.008	0.000	0.009	-0.060
Rise	0.000	0.008	9.340	0.000	-0.145	-0.259
Tilt	-0.060	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.009	-0.145	0.000	0.025	0.008
Twist	0.000	-0.060	-0.259	0.000	0.008	0.018

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.660	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	2.976	-0.166	0.000	0.013	-0.060
Rise	0.000	-0.166	9.857	0.000	-0.157	-0.268
Tilt	-0.067	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.013	-0.157	0.000	0.025	0.007
Twist	0.000	-0.060	-0.268	0.000	0.007	0.018

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.174	0.000	0.000	-0.134	0.000	0.000
Slide	0.000	4.045	2.968	0.000	0.012	-0.171
Rise	0.000	2.968	10.352	0.000	0.097	-0.202
Tilt	-0.134	0.000	0.000	0.046	0.000	0.000
Roll	0.000	0.012	0.097	0.000	0.029	0.009
Twist	0.000	-0.171	-0.202	0.000	0.009	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.450	0.000	0.000	-0.134	0.000	0.000
Slide	0.000	2.689	2.363	0.000	0.059	-0.107
Rise	0.000	2.363	10.599	0.000	0.109	-0.221
Tilt	-0.134	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.059	0.109	0.000	0.024	0.007
Twist	0.000	-0.107	-0.221	0.000	0.007	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.554	0.000	0.000	-0.142	0.000	0.000
Slide	0.000	2.829	2.524	0.000	0.055	-0.116
Rise	0.000	2.525	10.467	0.000	0.120	-0.220
Tilt	-0.142	0.000	0.000	0.044	0.000	0.000
Roll	0.000	0.055	0.120	0.000	0.026	0.008
Twist	0.000	-0.116	-0.220	0.000	0.008	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.118	0.000	0.000	-0.111	0.000	0.000
Slide	0.000	3.996	2.975	0.000	0.010	-0.154
Rise	0.000	2.975	10.595	0.000	0.105	-0.192
Tilt	-0.111	0.000	0.000	0.043	0.000	0.000
Roll	0.000	0.010	0.105	0.000	0.031	0.010
Twist	0.000	-0.154	-0.192	0.000	0.010	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.960	0.000	0.000	-0.105	0.000	0.000
Slide	0.000	2.275	1.757	0.000	-0.008	-0.172
Rise	0.000	1.757	8.777	0.000	-0.009	-0.187
Tilt	-0.105	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.008	-0.009	0.000	0.022	0.008
Twist	0.000	-0.172	-0.187	0.000	0.008	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.993	0.000	0.000	-0.124	0.000	0.000
Slide	0.000	2.075	1.547	0.000	-0.013	-0.153
Rise	0.000	1.548	8.553	0.000	-0.023	-0.206
Tilt	-0.124	0.000	0.000	0.042	0.000	0.000
Roll	0.000	-0.013	-0.023	0.000	0.022	0.010
Twist	0.000	-0.153	-0.206	0.000	0.010	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.952	0.000	0.000	-0.100	0.000	0.000
Slide	0.000	2.255	1.705	0.000	-0.008	-0.188
Rise	0.000	1.705	8.542	0.000	-0.011	-0.178
Tilt	-0.100	0.000	0.000	0.042	0.000	0.000
Roll	0.000	-0.008	-0.011	0.000	0.022	0.008
Twist	0.000	-0.188	-0.178	0.000	0.008	0.039

[Image courtesy of Curves+]

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	Mean	Stdev
-ccgg.12.cte	0.000	0.000

	Mean	Stdev
-ccgg.4.cte	0.000	0.000

	Mean	Stdev
-ccgg.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.13.cte	0.000	0.000

	Mean	Stdev
-cgcg.16.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.5.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-gcgc.11.cte	0.000	0.000

	Mean	Stdev
-gcgc.15.cte	0.000	0.000

	Mean	Stdev
-gcgc.3.cte	0.000	0.000

	Mean	Stdev
-gcgc.7.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.502	0.284

	Mean	Stdev
roll-10-GGCC	2.621	5.476

	Mean	Stdev
shift-10-GGCC	0.000	0.912

	Mean	Stdev
slide-10-GGCC	-0.230	0.667

	Mean	Stdev
tilt-10-GGCC	0.000	4.292

	Mean	Stdev
twist-10-GGCC	36.324	5.456

	Mean	Stdev
rise-11-GCGC	3.482	0.279

	Mean	Stdev
roll-11-GCGC	0.110	4.815

	Mean	Stdev
shift-11-GCGC	0.000	0.573

	Mean	Stdev
slide-11-GCGC	-0.717	0.455

	Mean	Stdev
tilt-11-GCGC	0.000	3.921

	Mean	Stdev
twist-11-GCGC	35.456	4.495

	Mean	Stdev
rise-12-CCGG	3.512	0.287

	Mean	Stdev
roll-12-CCGG	2.726	5.440

	Mean	Stdev
shift-12-CCGG	0.000	0.909

	Mean	Stdev
slide-12-CCGG	-0.241	0.671

	Mean	Stdev
tilt-12-CCGG	0.000	4.215

	Mean	Stdev
twist-12-CCGG	36.273	5.512

	Mean	Stdev
rise-13-CGCG	2.824	0.351

	Mean	Stdev
roll-13-CGCG	8.672	5.368

	Mean	Stdev
shift-13-CGCG	0.000	0.655

	Mean	Stdev
slide-13-CGCG	-0.018	0.504

	Mean	Stdev
tilt-13-CGCG	0.000	4.674

	Mean	Stdev
twist-13-CGCG	23.907	8.519

	Mean	Stdev
rise-14-GGCC	3.470	0.289

	Mean	Stdev
roll-14-GGCC	2.491	5.585

	Mean	Stdev
shift-14-GGCC	0.000	0.967

	Mean	Stdev
slide-14-GGCC	-0.147	0.669

	Mean	Stdev
tilt-14-GGCC	0.000	4.718

	Mean	Stdev
twist-14-GGCC	36.840	5.547

	Mean	Stdev
rise-15-GCGC	3.436	0.280

	Mean	Stdev
roll-15-GCGC	-0.952	5.553

	Mean	Stdev
shift-15-GCGC	0.000	0.755

	Mean	Stdev
slide-15-GCGC	-0.397	0.555

	Mean	Stdev
tilt-15-GCGC	0.000	4.415

	Mean	Stdev
twist-15-GCGC	34.475	5.112

	Mean	Stdev
rise-16-CGCG	3.154	0.640

	Mean	Stdev
roll-16-CGCG	6.785	10.483

	Mean	Stdev
shift-16-CGCG	0.000	1.267

	Mean	Stdev
slide-16-CGCG	0.131	0.884

	Mean	Stdev
tilt-16-CGCG	0.000	6.982

	Mean	Stdev
twist-16-CGCG	29.262	13.447

	Mean	Stdev
rise-2-CGCG	3.306	0.375

	Mean	Stdev
roll-2-CGCG	8.749	6.171

	Mean	Stdev
shift-2-CGCG	0.000	0.818

	Mean	Stdev
slide-2-CGCG	-0.054	0.546

	Mean	Stdev
tilt-2-CGCG	0.000	5.393

	Mean	Stdev
twist-2-CGCG	34.318	7.710

	Mean	Stdev
rise-3-GCGC	3.427	0.286

	Mean	Stdev
roll-3-GCGC	-0.384	5.464

	Mean	Stdev
shift-3-GCGC	0.000	0.733

	Mean	Stdev
slide-3-GCGC	-0.462	0.545

	Mean	Stdev
tilt-3-GCGC	0.000	4.373

	Mean	Stdev
twist-3-GCGC	34.350	5.111

	Mean	Stdev
rise-4-CCGG	3.463	0.287

	Mean	Stdev
roll-4-CCGG	2.663	5.701

	Mean	Stdev
shift-4-CCGG	0.000	0.958

	Mean	Stdev
slide-4-CCGG	-0.172	0.679

	Mean	Stdev
tilt-4-CCGG	0.000	4.676

	Mean	Stdev
twist-4-CCGG	36.607	5.692

	Mean	Stdev
rise-5-CGCG	2.824	0.332

	Mean	Stdev
roll-5-CGCG	8.673	5.340

	Mean	Stdev
shift-5-CGCG	0.000	0.661

	Mean	Stdev
slide-5-CGCG	-0.039	0.501

	Mean	Stdev
tilt-5-CGCG	0.000	4.655

	Mean	Stdev
twist-5-CGCG	23.919	8.134

	Mean	Stdev
rise-6-GGCC	3.524	0.285

	Mean	Stdev
roll-6-GGCC	2.885	5.440

	Mean	Stdev
shift-6-GGCC	0.000	0.906

	Mean	Stdev
slide-6-GGCC	-0.273	0.697

	Mean	Stdev
tilt-6-GGCC	0.000	4.325

	Mean	Stdev
twist-6-GGCC	36.188	5.398

	Mean	Stdev
rise-7-GCGC	3.465	0.277

	Mean	Stdev
roll-7-GCGC	0.056	4.753

	Mean	Stdev
shift-7-GCGC	0.000	0.565

	Mean	Stdev
slide-7-GCGC	-0.736	0.452

	Mean	Stdev
tilt-7-GCGC	0.000	3.947

	Mean	Stdev
twist-7-GCGC	35.360	4.572

	Mean	Stdev
rise-8-CCGG	3.502	0.285

	Mean	Stdev
roll-8-CCGG	2.525	5.488

	Mean	Stdev
shift-8-CCGG	0.000	0.911

	Mean	Stdev
slide-8-CCGG	-0.288	0.695

	Mean	Stdev
tilt-8-CCGG	0.000	4.268

	Mean	Stdev
twist-8-CCGG	35.664	5.953

	Mean	Stdev
rise-9-CGCG	2.775	0.326

	Mean	Stdev
roll-9-CGCG	8.775	5.177

	Mean	Stdev
shift-9-CGCG	0.000	0.623

	Mean	Stdev
slide-9-CGCG	-0.054	0.478

	Mean	Stdev
tilt-9-CGCG	0.000	4.656

	Mean	Stdev
twist-9-CGCG	23.087	7.930

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