NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.543	0.000	0.000	-0.023	0.000	0.000
Slide	0.000	2.243	1.250	0.000	-0.103	-0.117
Rise	0.000	1.250	7.405	0.000	-0.063	-0.167
Tilt	-0.023	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.103	-0.063	0.000	0.024	0.011
Twist	0.000	-0.117	-0.167	0.000	0.011	0.025

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.468	0.000	0.000	-0.038	0.000	0.000
Slide	0.000	2.435	0.947	0.000	-0.084	-0.166
Rise	0.000	0.947	7.765	0.000	-0.053	-0.158
Tilt	-0.038	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.084	-0.053	0.000	0.022	0.012
Twist	0.000	-0.166	-0.158	0.000	0.012	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.975	0.000	0.000	-0.041	0.000	0.000
Slide	0.000	2.557	0.970	0.000	-0.113	-0.128
Rise	0.000	0.970	7.650	0.000	-0.051	-0.177
Tilt	-0.041	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.113	-0.051	0.000	0.026	0.011
Twist	0.000	-0.128	-0.177	0.000	0.011	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.101	0.000	0.000	-0.068	0.000	0.000
Slide	0.000	3.417	0.625	0.000	-0.063	-0.238
Rise	0.000	0.625	7.855	0.000	-0.023	-0.165
Tilt	-0.068	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.063	-0.023	0.000	0.023	0.010
Twist	0.000	-0.238	-0.165	0.000	0.010	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.329	0.000	0.000	0.021	0.000	0.000
Slide	0.000	1.290	0.332	0.000	0.000	0.010
Rise	0.000	0.332	6.042	0.000	0.045	-0.064
Tilt	0.021	0.000	0.000	0.013	0.000	0.000
Roll	0.000	0.000	0.045	0.000	0.014	-0.001
Twist	0.000	0.010	-0.064	0.000	-0.001	0.009

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.734	0.000	0.000	-0.030	0.000	0.000
Slide	0.000	2.302	1.196	0.000	-0.097	-0.114
Rise	0.000	1.195	7.041	0.000	-0.051	-0.162
Tilt	-0.030	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.097	-0.051	0.000	0.023	0.011
Twist	0.000	-0.114	-0.162	0.000	0.011	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.558	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	2.665	1.050	0.000	-0.093	-0.189
Rise	0.000	1.050	7.995	0.000	-0.069	-0.175
Tilt	-0.037	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.093	-0.069	0.000	0.022	0.013
Twist	0.000	-0.189	-0.175	0.000	0.013	0.039

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.342	0.000	0.000	-0.051	0.000	0.000
Slide	0.000	1.927	1.478	0.000	-0.095	-0.061
Rise	0.000	1.478	7.009	0.000	-0.058	-0.177
Tilt	-0.051	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.095	-0.058	0.000	0.024	0.007
Twist	0.000	-0.061	-0.177	0.000	0.007	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.040	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	3.772	1.633	0.000	-0.080	-0.205
Rise	0.000	1.633	7.704	0.000	-0.063	-0.136
Tilt	-0.094	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.080	-0.063	0.000	0.027	0.005
Twist	0.000	-0.205	-0.136	0.000	0.005	0.043

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.931	0.000	0.000	-0.007	0.000	0.000
Slide	0.000	2.028	1.561	0.000	-0.072	-0.073
Rise	0.000	1.561	6.364	0.000	-0.041	-0.172
Tilt	-0.007	0.000	0.000	0.018	0.000	0.000
Roll	0.000	-0.072	-0.041	0.000	0.021	0.006
Twist	0.000	-0.073	-0.172	0.000	0.006	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.347	0.000	0.000	-0.048	0.000	0.000
Slide	0.000	1.818	1.481	0.000	-0.082	-0.064
Rise	0.000	1.481	6.519	0.000	-0.060	-0.167
Tilt	-0.048	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.082	-0.060	0.000	0.022	0.007
Twist	0.000	-0.064	-0.167	0.000	0.007	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.546	0.000	0.000	0.043	0.000	0.000
Slide	0.000	0.405	-0.001	0.000	-0.004	0.010
Rise	0.000	-0.001	1.484	0.000	-0.031	-0.021
Tilt	0.043	0.000	0.000	0.014	0.000	0.000
Roll	0.000	-0.004	-0.031	0.000	0.007	-0.000
Twist	0.000	0.010	-0.021	0.000	-0.000	0.001

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.370	0.000	0.000	-0.042	0.000	0.000
Slide	0.000	1.717	1.234	0.000	-0.042	-0.129
Rise	0.000	1.234	7.524	0.000	-0.046	-0.175
Tilt	-0.042	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.042	-0.046	0.000	0.022	0.012
Twist	0.000	-0.129	-0.175	0.000	0.012	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.333	0.000	0.000	-0.043	0.000	0.000
Slide	0.000	1.750	1.269	0.000	-0.036	-0.125
Rise	0.000	1.269	6.592	0.000	-0.046	-0.163
Tilt	-0.043	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.036	-0.046	0.000	0.021	0.011
Twist	0.000	-0.125	-0.163	0.000	0.011	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.309	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	1.661	1.262	0.000	-0.045	-0.122
Rise	0.000	1.262	6.879	0.000	-0.047	-0.172
Tilt	-0.037	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.045	-0.047	0.000	0.021	0.012
Twist	0.000	-0.122	-0.172	0.000	0.012	0.027

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.11.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.15.cte	0.000	0.000

	Mean	Stdev
-aatt.3.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-aatt.7.cte	0.000	0.000

	Mean	Stdev
-agct.12.cte	0.000	0.000

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-agct.4.cte	0.000	0.000

	Mean	Stdev
-agct.8.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-gatc.13.cte	0.000	0.000

	Mean	Stdev
-gatc.5.cte	0.000	0.000

	Mean	Stdev
-gatc.9.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.269	0.310

	Mean	Stdev
roll-10-AATT	1.872	5.773

	Mean	Stdev
shift-10-AATT	0.000	0.620

	Mean	Stdev
slide-10-AATT	-0.452	0.621

	Mean	Stdev
tilt-10-AATT	0.000	4.280

	Mean	Stdev
twist-10-AATT	32.384	6.158

	Mean	Stdev
rise-11-AATT	3.257	0.289

	Mean	Stdev
roll-11-AATT	1.139	5.810

	Mean	Stdev
shift-11-AATT	0.000	0.644

	Mean	Stdev
slide-11-AATT	-0.453	0.605

	Mean	Stdev
tilt-11-AATT	0.000	4.763

	Mean	Stdev
twist-11-AATT	34.180	5.279

	Mean	Stdev
rise-12-AGCT	3.364	0.343

	Mean	Stdev
roll-12-AGCT	4.517	5.730

	Mean	Stdev
shift-12-AGCT	0.000	0.680

	Mean	Stdev
slide-12-AGCT	-0.711	0.666

	Mean	Stdev
tilt-12-AGCT	0.000	4.218

	Mean	Stdev
twist-12-AGCT	31.033	5.864

	Mean	Stdev
rise-13-GATC	3.380	0.306

	Mean	Stdev
roll-13-GATC	2.212	5.788

	Mean	Stdev
shift-13-GATC	0.000	0.668

	Mean	Stdev
slide-13-GATC	-0.415	0.729

	Mean	Stdev
tilt-13-GATC	0.000	4.469

	Mean	Stdev
twist-13-GATC	35.008	6.310

	Mean	Stdev
rise-14-AATT	3.269	0.300

	Mean	Stdev
roll-14-AATT	0.885	5.524

	Mean	Stdev
shift-14-AATT	0.000	0.552

	Mean	Stdev
slide-14-AATT	-0.460	0.571

	Mean	Stdev
tilt-14-AATT	0.000	4.202

	Mean	Stdev
twist-14-AATT	32.659	5.607

	Mean	Stdev
rise-15-AATT	3.330	0.285

	Mean	Stdev
roll-15-AATT	-0.373	5.289

	Mean	Stdev
shift-15-AATT	0.000	0.549

	Mean	Stdev
slide-15-AATT	-0.458	0.519

	Mean	Stdev
tilt-15-AATT	0.000	4.610

	Mean	Stdev
twist-15-AATT	35.639	4.709

	Mean	Stdev
rise-16-AGCT	3.448	0.292

	Mean	Stdev
roll-16-AGCT	2.139	4.833

	Mean	Stdev
shift-16-AGCT	0.000	0.570

	Mean	Stdev
slide-16-AGCT	-0.887	0.478

	Mean	Stdev
tilt-16-AGCT	0.000	4.255

	Mean	Stdev
twist-16-AGCT	34.509	4.324

	Mean	Stdev
rise-2-CATG	3.624	0.810

	Mean	Stdev
roll-2-CATG	6.482	10.034

	Mean	Stdev
shift-2-CATG	0.000	1.187

	Mean	Stdev
slide-2-CATG	0.456	1.383

	Mean	Stdev
tilt-2-CATG	0.000	7.333

	Mean	Stdev
twist-2-CATG	35.692	28.996

	Mean	Stdev
rise-3-AATT	3.214	0.331

	Mean	Stdev
roll-3-AATT	3.819	6.601

	Mean	Stdev
shift-3-AATT	0.000	1.409

	Mean	Stdev
slide-3-AATT	0.092	0.685

	Mean	Stdev
tilt-3-AATT	0.000	7.205

	Mean	Stdev
twist-3-AATT	37.048	8.641

	Mean	Stdev
rise-4-AGCT	3.370	0.365

	Mean	Stdev
roll-4-AGCT	4.815	5.803

	Mean	Stdev
shift-4-AGCT	0.000	0.795

	Mean	Stdev
slide-4-AGCT	-0.634	0.638

	Mean	Stdev
tilt-4-AGCT	0.000	5.766

	Mean	Stdev
twist-4-AGCT	30.963	6.146

	Mean	Stdev
rise-5-GATC	3.423	0.330

	Mean	Stdev
roll-5-GATC	2.608	6.035

	Mean	Stdev
shift-5-GATC	0.000	0.680

	Mean	Stdev
slide-5-GATC	-0.425	0.720

	Mean	Stdev
tilt-5-GATC	0.000	4.596

	Mean	Stdev
twist-5-GATC	35.036	6.309

	Mean	Stdev
rise-6-AATT	3.291	0.316

	Mean	Stdev
roll-6-AATT	1.860	5.842

	Mean	Stdev
shift-6-AATT	0.000	0.586

	Mean	Stdev
slide-6-AATT	-0.445	0.601

	Mean	Stdev
tilt-6-AATT	0.000	4.276

	Mean	Stdev
twist-6-AATT	32.630	5.798

	Mean	Stdev
rise-7-AATT	3.261	0.286

	Mean	Stdev
roll-7-AATT	1.106	5.847

	Mean	Stdev
shift-7-AATT	0.000	0.624

	Mean	Stdev
slide-7-AATT	-0.460	0.590

	Mean	Stdev
tilt-7-AATT	0.000	4.702

	Mean	Stdev
twist-7-AATT	34.390	5.076

	Mean	Stdev
rise-8-AGCT	3.387	0.355

	Mean	Stdev
roll-8-AGCT	4.401	5.873

	Mean	Stdev
shift-8-AGCT	0.000	0.677

	Mean	Stdev
slide-8-AGCT	-0.766	0.683

	Mean	Stdev
tilt-8-AGCT	0.000	4.293

	Mean	Stdev
twist-8-AGCT	31.110	5.651

	Mean	Stdev
rise-9-GATC	3.380	0.326

	Mean	Stdev
roll-9-GATC	2.960	6.084

	Mean	Stdev
shift-9-GATC	0.000	0.682

	Mean	Stdev
slide-9-GATC	-0.444	0.743

	Mean	Stdev
tilt-9-GATC	0.000	4.592

	Mean	Stdev
twist-9-GATC	34.280	6.646

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