NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

Open in New Window

Download Raw Data

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.579	0.000	0.000	-0.056	0.000	0.000
Slide	0.000	3.111	1.349	0.000	-0.077	-0.201
Rise	0.000	1.349	7.471	0.000	-0.053	-0.178
Tilt	-0.056	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.077	-0.053	0.000	0.024	0.011
Twist	0.000	-0.201	-0.178	0.000	0.011	0.039

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.405	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	2.984	1.053	0.000	-0.089	-0.173
Rise	0.000	1.053	8.080	0.000	-0.056	-0.161
Tilt	-0.037	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.089	-0.056	0.000	0.023	0.011
Twist	0.000	-0.173	-0.161	0.000	0.011	0.035

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.563	0.000	0.000	-0.057	0.000	0.000
Slide	0.000	2.897	1.346	0.000	-0.085	-0.174
Rise	0.000	1.346	7.425	0.000	-0.052	-0.186
Tilt	-0.057	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.085	-0.052	0.000	0.023	0.012
Twist	0.000	-0.174	-0.186	0.000	0.012	0.037

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.893	0.000	0.000	-0.019	0.000	0.000
Slide	0.000	3.525	2.402	0.000	0.037	-0.153
Rise	0.000	2.402	9.108	0.000	0.063	-0.218
Tilt	-0.019	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.037	0.063	0.000	0.021	0.009
Twist	0.000	-0.153	-0.218	0.000	0.009	0.044

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.505	0.000	0.000	-0.062	0.000	0.000
Slide	0.000	3.599	2.099	0.000	0.029	-0.156
Rise	0.000	2.099	9.485	0.000	0.071	-0.230
Tilt	-0.062	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.029	0.071	0.000	0.026	0.011
Twist	0.000	-0.156	-0.230	0.000	0.011	0.044

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.935	0.000	0.000	-0.027	0.000	0.000
Slide	0.000	3.233	2.698	0.000	0.030	-0.107
Rise	0.000	2.698	9.315	0.000	0.058	-0.251
Tilt	-0.027	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.030	0.058	0.000	0.021	0.009
Twist	0.000	-0.107	-0.251	0.000	0.009	0.032

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.852	0.000	0.000	-0.023	0.000	0.000
Slide	0.000	3.485	2.701	0.000	0.034	-0.144
Rise	0.000	2.701	9.538	0.000	0.065	-0.237
Tilt	-0.023	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.034	0.065	0.000	0.022	0.009
Twist	0.000	-0.144	-0.238	0.000	0.009	0.041

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.751	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	1.900	0.907	0.000	-0.004	-0.075
Rise	0.000	0.907	5.859	0.000	-0.114	-0.195
Tilt	-0.026	0.000	0.000	0.023	0.000	0.000
Roll	0.000	-0.004	-0.114	0.000	0.014	0.008
Twist	0.000	-0.075	-0.195	0.000	0.008	0.021

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.347	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	2.019	0.803	0.000	-0.006	-0.057
Rise	0.000	0.803	6.276	0.000	-0.108	-0.232
Tilt	-0.095	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.006	-0.108	0.000	0.019	0.007
Twist	0.000	-0.057	-0.232	0.000	0.007	0.015

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.098	0.000	0.000	0.009	0.000	0.000
Slide	0.000	1.089	-0.037	0.000	-0.017	-0.021
Rise	0.000	-0.037	1.588	0.000	-0.026	-0.062
Tilt	0.009	0.000	0.000	0.005	0.000	0.000
Roll	0.000	-0.016	-0.026	0.000	0.003	-0.001
Twist	0.000	-0.021	-0.062	0.000	-0.001	0.008

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.197	0.000	0.000	-0.101	0.000	0.000
Slide	0.000	1.831	0.833	0.000	0.011	-0.054
Rise	0.000	0.833	6.544	0.000	-0.103	-0.232
Tilt	-0.101	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.011	-0.103	0.000	0.018	0.007
Twist	0.000	-0.054	-0.232	0.000	0.007	0.015

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.287	0.000	0.000	-0.092	0.000	0.000
Slide	0.000	1.970	0.766	0.000	0.002	-0.051
Rise	0.000	0.766	6.453	0.000	-0.101	-0.234
Tilt	-0.092	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.002	-0.101	0.000	0.019	0.007
Twist	0.000	-0.051	-0.234	0.000	0.007	0.015

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.936	0.000	0.000	-0.052	0.000	0.000
Slide	0.000	1.764	1.163	0.000	-0.044	-0.078
Rise	0.000	1.163	6.744	0.000	-0.043	-0.174
Tilt	-0.052	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.044	-0.043	0.000	0.024	0.011
Twist	0.000	-0.078	-0.174	0.000	0.011	0.019

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.969	0.000	0.000	-0.058	0.000	0.000
Slide	0.000	1.733	1.268	0.000	-0.033	-0.104
Rise	0.000	1.268	6.894	0.000	-0.044	-0.190
Tilt	-0.058	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.033	-0.044	0.000	0.023	0.012
Twist	0.000	-0.104	-0.190	0.000	0.012	0.025

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.973	0.000	0.000	-0.056	0.000	0.000
Slide	0.000	1.764	1.333	0.000	-0.043	-0.110
Rise	0.000	1.333	7.096	0.000	-0.046	-0.204
Tilt	-0.056	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.043	-0.046	0.000	0.023	0.012
Twist	0.000	-0.110	-0.204	0.000	0.012	0.024

[Image courtesy of Curves+]

Download Raw Data

Open in New Window

	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-acgt.11.cte	0.000	0.000

	Mean	Stdev
-acgt.15.cte	0.000	0.000

	Mean	Stdev
-acgt.3.cte	0.000	0.000

	Mean	Stdev
-acgt.7.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.12.cte	0.000	0.000

	Mean	Stdev
-cgcg.16.cte	0.000	0.000

	Mean	Stdev
-cgcg.4.cte	0.000	0.000

	Mean	Stdev
-cgcg.8.cte	0.000	0.000

	Mean	Stdev
-gatc.13.cte	0.000	0.000

	Mean	Stdev
-gatc.5.cte	0.000	0.000

	Mean	Stdev
-gatc.9.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.353	0.299

	Mean	Stdev
roll-10-AATT	1.240	5.348

	Mean	Stdev
shift-10-AATT	0.000	0.633

	Mean	Stdev
slide-10-AATT	-0.378	0.537

	Mean	Stdev
tilt-10-AATT	0.000	4.790

	Mean	Stdev
twist-10-AATT	35.640	4.982

	Mean	Stdev
rise-11-ACGT	3.433	0.293

	Mean	Stdev
roll-11-ACGT	-0.938	5.870

	Mean	Stdev
shift-11-ACGT	0.000	0.814

	Mean	Stdev
slide-11-ACGT	-0.653	0.481

	Mean	Stdev
tilt-11-ACGT	0.000	3.860

	Mean	Stdev
twist-11-ACGT	32.863	4.478

	Mean	Stdev
rise-12-CGCG	3.096	0.466

	Mean	Stdev
roll-12-CGCG	10.201	6.153

	Mean	Stdev
shift-12-CGCG	0.000	0.770

	Mean	Stdev
slide-12-CGCG	-0.070	0.575

	Mean	Stdev
tilt-12-CGCG	0.000	5.645

	Mean	Stdev
twist-12-CGCG	27.586	10.332

	Mean	Stdev
rise-13-GATC	3.381	0.351

	Mean	Stdev
roll-13-GATC	2.425	6.048

	Mean	Stdev
shift-13-GATC	0.000	0.831

	Mean	Stdev
slide-13-GATC	0.049	0.648

	Mean	Stdev
tilt-13-GATC	0.000	4.582

	Mean	Stdev
twist-13-GATC	34.881	8.132

	Mean	Stdev
rise-14-AATT	3.292	0.283

	Mean	Stdev
roll-14-AATT	0.901	5.547

	Mean	Stdev
shift-14-AATT	0.000	0.657

	Mean	Stdev
slide-14-AATT	-0.293	0.532

	Mean	Stdev
tilt-14-AATT	0.000	4.615

	Mean	Stdev
twist-14-AATT	35.332	5.146

	Mean	Stdev
rise-15-ACGT	3.410	0.283

	Mean	Stdev
roll-15-ACGT	-0.504	5.282

	Mean	Stdev
shift-15-ACGT	0.000	0.645

	Mean	Stdev
slide-15-ACGT	-0.667	0.462

	Mean	Stdev
tilt-15-ACGT	0.000	3.915

	Mean	Stdev
twist-15-ACGT	32.539	4.491

	Mean	Stdev
rise-16-CGCG	3.181	0.988

	Mean	Stdev
roll-16-CGCG	-2.834	18.918

	Mean	Stdev
shift-16-CGCG	0.000	2.660

	Mean	Stdev
slide-16-CGCG	-0.287	0.902

	Mean	Stdev
tilt-16-CGCG	0.000	11.756

	Mean	Stdev
twist-16-CGCG	29.075	13.809

	Mean	Stdev
rise-2-CATG	3.298	0.390

	Mean	Stdev
roll-2-CATG	8.383	7.425

	Mean	Stdev
shift-2-CATG	0.000	0.901

	Mean	Stdev
slide-2-CATG	-0.231	0.614

	Mean	Stdev
tilt-2-CATG	0.000	5.177

	Mean	Stdev
twist-2-CATG	34.083	7.038

	Mean	Stdev
rise-3-ACGT	3.320	0.323

	Mean	Stdev
roll-3-ACGT	0.173	6.089

	Mean	Stdev
shift-3-ACGT	0.000	0.800

	Mean	Stdev
slide-3-ACGT	-0.531	0.499

	Mean	Stdev
tilt-3-ACGT	0.000	4.070

	Mean	Stdev
twist-3-ACGT	30.485	5.631

	Mean	Stdev
rise-4-CGCG	3.211	0.438

	Mean	Stdev
roll-4-CGCG	9.598	6.368

	Mean	Stdev
shift-4-CGCG	0.000	0.859

	Mean	Stdev
slide-4-CGCG	-0.008	0.612

	Mean	Stdev
tilt-4-CGCG	0.000	5.900

	Mean	Stdev
twist-4-CGCG	31.196	9.735

	Mean	Stdev
rise-5-GATC	3.430	0.336

	Mean	Stdev
roll-5-GATC	2.331	5.948

	Mean	Stdev
shift-5-GATC	0.000	0.825

	Mean	Stdev
slide-5-GATC	-0.043	0.685

	Mean	Stdev
tilt-5-GATC	0.000	4.578

	Mean	Stdev
twist-5-GATC	35.250	6.811

	Mean	Stdev
rise-6-AATT	3.338	0.304

	Mean	Stdev
roll-6-AATT	1.485	5.606

	Mean	Stdev
shift-6-AATT	0.000	0.637

	Mean	Stdev
slide-6-AATT	-0.373	0.543

	Mean	Stdev
tilt-6-AATT	0.000	4.827

	Mean	Stdev
twist-6-AATT	35.454	5.118

	Mean	Stdev
rise-7-ACGT	3.404	0.297

	Mean	Stdev
roll-7-ACGT	-0.991	5.818

	Mean	Stdev
shift-7-ACGT	0.000	0.837

	Mean	Stdev
slide-7-ACGT	-0.638	0.488

	Mean	Stdev
tilt-7-ACGT	0.000	3.988

	Mean	Stdev
twist-7-ACGT	32.497	4.688

	Mean	Stdev
rise-8-CGCG	3.114	0.463

	Mean	Stdev
roll-8-CGCG	10.307	6.112

	Mean	Stdev
shift-8-CGCG	0.000	0.790

	Mean	Stdev
slide-8-CGCG	-0.077	0.577

	Mean	Stdev
tilt-8-CGCG	0.000	5.732

	Mean	Stdev
twist-8-CGCG	27.782	10.411

	Mean	Stdev
rise-9-GATC	3.421	0.340

	Mean	Stdev
roll-9-GATC	2.367	5.962

	Mean	Stdev
shift-9-GATC	0.000	0.819

	Mean	Stdev
slide-9-GATC	-0.002	0.692

	Mean	Stdev
tilt-9-GATC	0.000	4.494

	Mean	Stdev
twist-9-GATC	35.385	7.295

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18
5'	G	-	C	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	A	-	A	-	C	-	G	-	C	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	C	-	G	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	T	-	T	-	G	-	C	-	G	5'
	36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21		20		19