NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.691	0.000	0.000	-0.024	0.000	0.000
Slide	0.000	3.405	1.569	0.000	-0.090	-0.243
Rise	0.000	1.569	7.025	0.000	-0.065	-0.183
Tilt	-0.024	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.090	-0.065	0.000	0.022	0.010
Twist	0.000	-0.243	-0.183	0.000	0.010	0.042

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.819	0.000	0.000	-0.045	0.000	0.000
Slide	0.000	3.277	1.117	0.000	-0.071	-0.241
Rise	0.000	1.117	7.530	0.000	-0.045	-0.150
Tilt	-0.045	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.071	-0.045	0.000	0.023	0.009
Twist	0.000	-0.241	-0.150	0.000	0.009	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.716	0.000	0.000	-0.031	0.000	0.000
Slide	0.000	3.393	1.616	0.000	-0.084	-0.249
Rise	0.000	1.616	7.355	0.000	-0.070	-0.185
Tilt	-0.031	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.084	-0.070	0.000	0.023	0.010
Twist	0.000	-0.249	-0.185	0.000	0.010	0.044

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.222	0.000	0.000	-0.000	0.000	0.000
Slide	0.000	4.129	2.721	0.000	-0.018	-0.177
Rise	0.000	2.721	10.291	0.000	0.059	-0.194
Tilt	-0.000	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.018	0.059	0.000	0.024	0.012
Twist	0.000	-0.177	-0.194	0.000	0.012	0.047

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.239	0.000	0.000	-0.008	0.000	0.000
Slide	0.000	2.695	1.466	0.000	-0.013	-0.023
Rise	0.000	1.466	9.272	0.000	0.051	-0.085
Tilt	-0.008	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.013	0.051	0.000	0.024	0.009
Twist	0.000	-0.023	-0.085	0.000	0.009	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.194	0.000	0.000	0.014	0.000	0.000
Slide	0.000	4.186	2.804	0.000	-0.042	-0.123
Rise	0.000	2.804	9.932	0.000	0.062	-0.188
Tilt	0.014	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.042	0.062	0.000	0.026	0.012
Twist	0.000	-0.123	-0.188	0.000	0.012	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.266	0.000	0.000	-0.000	0.000	0.000
Slide	0.000	4.295	2.661	0.000	-0.016	-0.182
Rise	0.000	2.661	10.211	0.000	0.084	-0.177
Tilt	-0.000	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.016	0.084	0.000	0.026	0.014
Twist	0.000	-0.182	-0.177	0.000	0.014	0.052

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.413	0.000	0.000	0.011	0.000	0.000
Slide	0.000	1.023	-0.241	0.000	0.002	-0.038
Rise	0.000	-0.241	1.568	0.000	-0.051	-0.018
Tilt	0.011	0.000	0.000	0.006	0.000	0.000
Roll	0.000	0.002	-0.051	0.000	0.008	0.000
Twist	0.000	-0.038	-0.018	0.000	0.000	0.006

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.990	0.000	0.000	-0.057	0.000	0.000
Slide	0.000	1.766	1.204	0.000	-0.027	-0.114
Rise	0.000	1.204	6.800	0.000	-0.039	-0.171
Tilt	-0.057	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.027	-0.039	0.000	0.022	0.011
Twist	0.000	-0.114	-0.171	0.000	0.011	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.021	0.000	0.000	-0.064	0.000	0.000
Slide	0.000	1.666	1.171	0.000	-0.029	-0.103
Rise	0.000	1.171	6.860	0.000	-0.048	-0.166
Tilt	-0.064	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.029	-0.048	0.000	0.022	0.012
Twist	0.000	-0.103	-0.166	0.000	0.012	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.013	0.000	0.000	-0.061	0.000	0.000
Slide	0.000	1.666	1.174	0.000	-0.027	-0.116
Rise	0.000	1.174	6.911	0.000	-0.025	-0.164
Tilt	-0.061	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.027	-0.025	0.000	0.021	0.011
Twist	0.000	-0.116	-0.164	0.000	0.011	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.991	0.000	0.000	-0.050	0.000	0.000
Slide	0.000	1.691	1.061	0.000	-0.006	-0.043
Rise	0.000	1.061	6.827	0.000	-0.120	-0.210
Tilt	-0.050	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.006	-0.120	0.000	0.020	0.009
Twist	0.000	-0.043	-0.210	0.000	0.009	0.016

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.177	0.000	0.000	-0.019	0.000	0.000
Slide	0.000	0.648	-0.096	0.000	0.002	0.038
Rise	0.000	-0.096	5.121	0.000	-0.078	-0.079
Tilt	-0.019	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.002	-0.078	0.000	0.015	0.002
Twist	0.000	0.038	-0.079	0.000	0.002	0.006

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.883	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	1.704	1.086	0.000	0.002	-0.055
Rise	0.000	1.086	6.249	0.000	-0.091	-0.195
Tilt	-0.055	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.002	-0.091	0.000	0.018	0.008
Twist	0.000	-0.055	-0.195	0.000	0.008	0.017

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.925	0.000	0.000	-0.060	0.000	0.000
Slide	0.000	1.712	1.123	0.000	-0.002	-0.044
Rise	0.000	1.123	6.154	0.000	-0.082	-0.191
Tilt	-0.060	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.002	-0.082	0.000	0.018	0.008
Twist	0.000	-0.044	-0.191	0.000	0.008	0.016

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-atat.11.cte	0.000	0.000

	Mean	Stdev
-atat.15.cte	0.000	0.000

	Mean	Stdev
-atat.3.cte	0.000	0.000

	Mean	Stdev
-atat.7.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-gatc.13.cte	0.000	0.000

	Mean	Stdev
-gatc.5.cte	0.000	0.000

	Mean	Stdev
-gatc.9.cte	0.000	0.000

	Mean	Stdev
-tgca.12.cte	0.000	0.000

	Mean	Stdev
-tgca.16.cte	0.000	0.000

	Mean	Stdev
-tgca.4.cte	0.000	0.000

	Mean	Stdev
-tgca.8.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.337	0.310

	Mean	Stdev
roll-10-AATT	1.202	5.480

	Mean	Stdev
shift-10-AATT	0.000	0.593

	Mean	Stdev
slide-10-AATT	-0.599	0.554

	Mean	Stdev
tilt-10-AATT	0.000	4.834

	Mean	Stdev
twist-10-AATT	34.484	5.009

	Mean	Stdev
rise-11-ATAT	3.282	0.272

	Mean	Stdev
roll-11-ATAT	-1.158	5.499

	Mean	Stdev
shift-11-ATAT	0.000	0.693

	Mean	Stdev
slide-11-ATAT	-0.793	0.439

	Mean	Stdev
tilt-11-ATAT	0.000	3.932

	Mean	Stdev
twist-11-ATAT	31.256	4.276

	Mean	Stdev
rise-12-TGCA	3.227	0.387

	Mean	Stdev
roll-12-TGCA	11.538	6.345

	Mean	Stdev
shift-12-TGCA	0.000	0.810

	Mean	Stdev
slide-12-TGCA	-0.191	0.627

	Mean	Stdev
tilt-12-TGCA	0.000	5.061

	Mean	Stdev
twist-12-TGCA	29.265	8.736

	Mean	Stdev
rise-13-GATC	3.437	0.328

	Mean	Stdev
roll-13-GATC	2.076	5.966

	Mean	Stdev
shift-13-GATC	0.000	0.814

	Mean	Stdev
slide-13-GATC	-0.184	0.701

	Mean	Stdev
tilt-13-GATC	0.000	4.602

	Mean	Stdev
twist-13-GATC	35.334	6.844

	Mean	Stdev
rise-14-AATT	3.357	0.290

	Mean	Stdev
roll-14-AATT	0.938	5.261

	Mean	Stdev
shift-14-AATT	0.000	0.581

	Mean	Stdev
slide-14-AATT	-0.523	0.543

	Mean	Stdev
tilt-14-AATT	0.000	4.888

	Mean	Stdev
twist-14-AATT	35.783	4.611

	Mean	Stdev
rise-15-ATAT	3.325	0.272

	Mean	Stdev
roll-15-ATAT	-1.850	5.472

	Mean	Stdev
shift-15-ATAT	0.000	0.688

	Mean	Stdev
slide-15-ATAT	-0.783	0.490

	Mean	Stdev
tilt-15-ATAT	0.000	3.878

	Mean	Stdev
twist-15-ATAT	31.218	5.298

	Mean	Stdev
rise-16-TGCA	3.207	0.408

	Mean	Stdev
roll-16-TGCA	8.794	6.487

	Mean	Stdev
shift-16-TGCA	0.000	0.710

	Mean	Stdev
slide-16-TGCA	0.179	1.261

	Mean	Stdev
tilt-16-TGCA	0.000	4.838

	Mean	Stdev
twist-16-TGCA	24.090	14.694

	Mean	Stdev
rise-2-CATG	3.294	0.741

	Mean	Stdev
roll-2-CATG	7.444	9.561

	Mean	Stdev
shift-2-CATG	0.000	1.220

	Mean	Stdev
slide-2-CATG	-0.441	0.909

	Mean	Stdev
tilt-2-CATG	0.012	9.808

	Mean	Stdev
twist-2-CATG	33.065	11.521

	Mean	Stdev
rise-3-ATAT	3.227	0.288

	Mean	Stdev
roll-3-ATAT	0.016	5.316

	Mean	Stdev
shift-3-ATAT	0.000	0.702

	Mean	Stdev
slide-3-ATAT	-0.716	0.427

	Mean	Stdev
tilt-3-ATAT	0.000	3.852

	Mean	Stdev
twist-3-ATAT	29.726	4.503

	Mean	Stdev
rise-4-TGCA	3.279	0.389

	Mean	Stdev
roll-4-TGCA	10.722	6.632

	Mean	Stdev
shift-4-TGCA	0.000	0.880

	Mean	Stdev
slide-4-TGCA	-0.149	0.643

	Mean	Stdev
tilt-4-TGCA	0.000	5.350

	Mean	Stdev
twist-4-TGCA	30.847	8.193

	Mean	Stdev
rise-5-GATC	3.415	0.325

	Mean	Stdev
roll-5-GATC	2.320	6.100

	Mean	Stdev
shift-5-GATC	0.000	0.812

	Mean	Stdev
slide-5-GATC	-0.185	0.703

	Mean	Stdev
tilt-5-GATC	0.000	4.642

	Mean	Stdev
twist-5-GATC	35.139	6.714

	Mean	Stdev
rise-6-AATT	3.339	0.303

	Mean	Stdev
roll-6-AATT	1.163	5.404

	Mean	Stdev
shift-6-AATT	0.000	0.591

	Mean	Stdev
slide-6-AATT	-0.567	0.554

	Mean	Stdev
tilt-6-AATT	0.000	4.908

	Mean	Stdev
twist-6-AATT	34.921	4.901

	Mean	Stdev
rise-7-ATAT	3.286	0.272

	Mean	Stdev
roll-7-ATAT	-1.532	5.300

	Mean	Stdev
shift-7-ATAT	0.000	0.680

	Mean	Stdev
slide-7-ATAT	-0.805	0.426

	Mean	Stdev
tilt-7-ATAT	0.000	3.800

	Mean	Stdev
twist-7-ATAT	31.171	4.013

	Mean	Stdev
rise-8-TGCA	3.260	0.398

	Mean	Stdev
roll-8-TGCA	10.966	6.524

	Mean	Stdev
shift-8-TGCA	0.000	0.865

	Mean	Stdev
slide-8-TGCA	-0.209	0.630

	Mean	Stdev
tilt-8-TGCA	0.000	5.271

	Mean	Stdev
twist-8-TGCA	29.998	8.235

	Mean	Stdev
rise-9-GATC	3.401	0.323

	Mean	Stdev
roll-9-GATC	2.449	6.100

	Mean	Stdev
shift-9-GATC	0.000	0.811

	Mean	Stdev
slide-9-GATC	-0.205	0.732

	Mean	Stdev
tilt-9-GATC	0.000	4.682

	Mean	Stdev
twist-9-GATC	34.883	6.730

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