NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.995	0.000	0.000	-0.081	0.000	0.000
Slide	0.000	2.962	0.971	0.000	-0.056	-0.218
Rise	0.000	0.971	8.827	0.000	0.008	-0.180
Tilt	-0.081	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.056	0.008	0.000	0.020	0.009
Twist	0.000	-0.218	-0.180	0.000	0.009	0.037

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.866	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	2.537	0.802	0.000	-0.053	-0.171
Rise	0.000	0.802	8.717	0.000	-0.009	-0.176
Tilt	-0.090	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.053	-0.009	0.000	0.020	0.011
Twist	0.000	-0.171	-0.176	0.000	0.011	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.983	0.000	0.000	-0.085	0.000	0.000
Slide	0.000	2.874	0.806	0.000	-0.057	-0.213
Rise	0.000	0.806	8.490	0.000	-0.007	-0.200
Tilt	-0.085	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.057	-0.007	0.000	0.020	0.011
Twist	0.000	-0.213	-0.200	0.000	0.011	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.714	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	4.145	2.531	0.000	0.028	-0.219
Rise	0.000	2.531	9.994	0.000	0.049	-0.205
Tilt	-0.026	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.028	0.049	0.000	0.027	0.006
Twist	0.000	-0.219	-0.205	0.000	0.006	0.056

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.011	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	2.173	1.659	0.000	0.022	-0.063
Rise	0.000	1.659	8.923	0.000	0.033	-0.196
Tilt	-0.067	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.022	0.033	0.000	0.024	0.011
Twist	0.000	-0.063	-0.196	0.000	0.011	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.282	0.000	0.000	-0.039	0.000	0.000
Slide	0.000	3.385	2.636	0.000	0.029	-0.177
Rise	0.000	2.636	9.831	0.000	0.079	-0.207
Tilt	-0.039	0.000	0.000	0.030	0.000	0.000
Roll	0.000	0.029	0.079	0.000	0.024	0.009
Twist	0.000	-0.177	-0.207	0.000	0.009	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.671	0.000	0.000	-0.029	0.000	0.000
Slide	0.000	4.346	2.879	0.000	0.027	-0.227
Rise	0.000	2.879	9.928	0.000	0.050	-0.219
Tilt	-0.029	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.027	0.050	0.000	0.027	0.006
Twist	0.000	-0.227	-0.219	0.000	0.006	0.055

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.081	0.000	0.000	-0.041	0.000	0.000
Slide	0.000	1.990	1.162	0.000	-0.003	-0.070
Rise	0.000	1.162	6.339	0.000	-0.085	-0.201
Tilt	-0.041	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.003	-0.085	0.000	0.018	0.009
Twist	0.000	-0.070	-0.201	0.000	0.009	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.189	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	0.840	0.023	0.000	0.021	0.036
Rise	0.000	0.023	4.130	0.000	-0.038	-0.063
Tilt	-0.090	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.021	-0.038	0.000	0.015	0.002
Twist	0.000	0.036	-0.063	0.000	0.002	0.005

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.736	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.692	1.448	0.000	-0.029	-0.197
Rise	0.000	1.448	7.760	0.000	-0.016	-0.187
Tilt	-0.088	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.029	-0.016	0.000	0.018	0.006
Twist	0.000	-0.197	-0.187	0.000	0.006	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.767	0.000	0.000	-0.075	0.000	0.000
Slide	0.000	2.401	1.471	0.000	-0.040	-0.180
Rise	0.000	1.471	7.884	0.000	-0.020	-0.189
Tilt	-0.075	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.040	-0.020	0.000	0.019	0.007
Twist	0.000	-0.180	-0.189	0.000	0.007	0.033

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.732	0.000	0.000	-0.086	0.000	0.000
Slide	0.000	2.686	1.398	0.000	-0.027	-0.199
Rise	0.000	1.398	7.689	0.000	-0.013	-0.192
Tilt	-0.086	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.027	-0.013	0.000	0.019	0.007
Twist	0.000	-0.199	-0.192	0.000	0.007	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.754	0.000	0.000	-0.001	0.000	0.000
Slide	0.000	1.298	0.568	0.000	0.028	-0.013
Rise	0.000	0.568	7.255	0.000	-0.124	-0.218
Tilt	-0.001	0.000	0.000	0.021	0.000	0.000
Roll	0.000	0.028	-0.124	0.000	0.021	0.012
Twist	0.000	-0.013	-0.218	0.000	0.012	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.784	0.000	0.000	-0.001	0.000	0.000
Slide	0.000	1.262	0.657	0.000	0.018	-0.020
Rise	0.000	0.657	7.428	0.000	-0.139	-0.226
Tilt	-0.001	0.000	0.000	0.023	0.000	0.000
Roll	0.000	0.018	-0.139	0.000	0.021	0.012
Twist	0.000	-0.020	-0.226	0.000	0.012	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.762	0.000	0.000	0.007	0.000	0.000
Slide	0.000	1.325	0.644	0.000	0.015	-0.021
Rise	0.000	0.645	7.507	0.000	-0.136	-0.213
Tilt	0.007	0.000	0.000	0.022	0.000	0.000
Roll	0.000	0.015	-0.136	0.000	0.021	0.012
Twist	0.000	-0.021	-0.213	0.000	0.012	0.019

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.10.cte	0.000	0.000

	Mean	Stdev
-aatt.14.cte	0.000	0.000

	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-acgt.11.cte	0.000	0.000

	Mean	Stdev
-acgt.15.cte	0.000	0.000

	Mean	Stdev
-acgt.3.cte	0.000	0.000

	Mean	Stdev
-acgt.7.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.16.cte	0.000	0.000

	Mean	Stdev
-ctag.12.cte	0.000	0.000

	Mean	Stdev
-ctag.4.cte	0.000	0.000

	Mean	Stdev
-ctag.8.cte	0.000	0.000

	Mean	Stdev
-tata.13.cte	0.000	0.000

	Mean	Stdev
-tata.5.cte	0.000	0.000

	Mean	Stdev
-tata.9.cte	0.000	0.000

	Mean	Stdev
rise-10-AATT	3.276	0.274

	Mean	Stdev
roll-10-AATT	-0.750	5.772

	Mean	Stdev
shift-10-AATT	0.000	0.876

	Mean	Stdev
slide-10-AATT	-0.081	0.591

	Mean	Stdev
tilt-10-AATT	0.000	5.190

	Mean	Stdev
twist-10-AATT	36.447	5.708

	Mean	Stdev
rise-11-ACGT	3.491	0.266

	Mean	Stdev
roll-11-ACGT	-1.050	4.822

	Mean	Stdev
shift-11-ACGT	0.000	0.588

	Mean	Stdev
slide-11-ACGT	-0.893	0.449

	Mean	Stdev
tilt-11-ACGT	0.000	3.956

	Mean	Stdev
twist-11-ACGT	33.463	3.761

	Mean	Stdev
rise-12-CTAG	3.411	0.296

	Mean	Stdev
roll-12-CTAG	1.674	5.902

	Mean	Stdev
shift-12-CTAG	0.000	1.095

	Mean	Stdev
slide-12-CTAG	-0.411	0.591

	Mean	Stdev
tilt-12-CTAG	0.000	5.297

	Mean	Stdev
twist-12-CTAG	36.282	4.990

	Mean	Stdev
rise-13-TATA	3.044	0.356

	Mean	Stdev
roll-13-TATA	11.194	6.901

	Mean	Stdev
shift-13-TATA	0.000	0.882

	Mean	Stdev
slide-13-TATA	-0.160	0.712

	Mean	Stdev
tilt-13-TATA	0.000	5.254

	Mean	Stdev
twist-13-TATA	24.795	8.242

	Mean	Stdev
rise-14-AATT	3.269	0.275

	Mean	Stdev
roll-14-AATT	-0.254	5.990

	Mean	Stdev
shift-14-AATT	0.000	1.003

	Mean	Stdev
slide-14-AATT	0.018	0.584

	Mean	Stdev
tilt-14-AATT	0.000	5.525

	Mean	Stdev
twist-14-AATT	36.789	5.477

	Mean	Stdev
rise-15-ACGT	3.394	0.292

	Mean	Stdev
roll-15-ACGT	-1.031	5.413

	Mean	Stdev
shift-15-ACGT	0.000	0.810

	Mean	Stdev
slide-15-ACGT	-0.500	0.568

	Mean	Stdev
tilt-15-ACGT	0.000	4.163

	Mean	Stdev
twist-15-ACGT	32.318	4.470

	Mean	Stdev
rise-16-CGCG	3.383	0.453

	Mean	Stdev
roll-16-CGCG	9.200	6.482

	Mean	Stdev
shift-16-CGCG	0.000	0.820

	Mean	Stdev
slide-16-CGCG	0.188	1.093

	Mean	Stdev
tilt-16-CGCG	0.000	5.610

	Mean	Stdev
twist-16-CGCG	30.407	16.043

	Mean	Stdev
rise-2-CATG	3.299	0.370

	Mean	Stdev
roll-2-CATG	8.879	6.411

	Mean	Stdev
shift-2-CATG	0.000	0.757

	Mean	Stdev
slide-2-CATG	-0.314	0.595

	Mean	Stdev
tilt-2-CATG	0.000	4.725

	Mean	Stdev
twist-2-CATG	32.986	7.043

	Mean	Stdev
rise-3-ACGT	3.443	0.282

	Mean	Stdev
roll-3-ACGT	-0.286	5.488

	Mean	Stdev
shift-3-ACGT	0.000	0.690

	Mean	Stdev
slide-3-ACGT	-0.772	0.510

	Mean	Stdev
tilt-3-ACGT	0.000	4.483

	Mean	Stdev
twist-3-ACGT	32.974	4.354

	Mean	Stdev
rise-4-CTAG	3.359	0.297

	Mean	Stdev
roll-4-CTAG	1.772	5.753

	Mean	Stdev
shift-4-CTAG	0.000	1.020

	Mean	Stdev
slide-4-CTAG	-0.456	0.648

	Mean	Stdev
tilt-4-CTAG	0.000	5.351

	Mean	Stdev
twist-4-CTAG	35.154	5.713

	Mean	Stdev
rise-5-TATA	3.071	0.354

	Mean	Stdev
roll-5-TATA	11.458	6.729

	Mean	Stdev
shift-5-TATA	0.000	0.865

	Mean	Stdev
slide-5-TATA	-0.193	0.718

	Mean	Stdev
tilt-5-TATA	0.000	5.087

	Mean	Stdev
twist-5-TATA	25.229	7.890

	Mean	Stdev
rise-6-AATT	3.287	0.282

	Mean	Stdev
roll-6-AATT	-0.565	5.889

	Mean	Stdev
shift-6-AATT	0.000	0.893

	Mean	Stdev
slide-6-AATT	-0.067	0.576

	Mean	Stdev
tilt-6-AATT	0.000	5.158

	Mean	Stdev
twist-6-AATT	36.782	5.347

	Mean	Stdev
rise-7-ACGT	3.485	0.274

	Mean	Stdev
roll-7-ACGT	-0.941	4.855

	Mean	Stdev
shift-7-ACGT	0.000	0.596

	Mean	Stdev
slide-7-ACGT	-0.868	0.447

	Mean	Stdev
tilt-7-ACGT	0.000	3.920

	Mean	Stdev
twist-7-ACGT	33.361	3.812

	Mean	Stdev
rise-8-CTAG	3.403	0.297

	Mean	Stdev
roll-8-CTAG	1.434	5.811

	Mean	Stdev
shift-8-CTAG	0.000	1.088

	Mean	Stdev
slide-8-CTAG	-0.414	0.594

	Mean	Stdev
tilt-8-CTAG	0.000	5.268

	Mean	Stdev
twist-8-CTAG	36.149	5.061

	Mean	Stdev
rise-9-TATA	3.035	0.343

	Mean	Stdev
roll-9-TATA	11.237	6.764

	Mean	Stdev
shift-9-TATA	0.000	0.878

	Mean	Stdev
slide-9-TATA	-0.184	0.696

	Mean	Stdev
tilt-9-TATA	0.000	5.146

	Mean	Stdev
twist-9-TATA	24.632	8.011

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