NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.065	0.000	0.000	-0.027	0.000	0.000
Slide	0.000	1.628	0.653	0.000	-0.063	-0.016
Rise	0.000	0.653	3.735	0.000	-0.028	-0.087
Tilt	-0.027	0.000	0.000	0.018	0.000	0.000
Roll	0.000	-0.063	-0.028	0.000	0.020	0.004
Twist	0.000	-0.016	-0.087	0.000	0.004	0.014

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.293	0.000	0.000	0.022	0.000	0.000
Slide	0.000	4.000	2.762	0.000	-0.025	-0.124
Rise	0.000	2.762	10.241	0.000	0.074	-0.179
Tilt	0.022	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.025	0.073	0.000	0.026	0.013
Twist	0.000	-0.124	-0.179	0.000	0.013	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.102	0.000	0.000	-0.016	0.000	0.000
Slide	0.000	4.577	2.408	0.000	-0.034	-0.112
Rise	0.000	2.408	9.938	0.000	0.053	-0.157
Tilt	-0.016	0.000	0.000	0.040	0.000	0.000
Roll	0.000	-0.034	0.053	0.000	0.027	0.012
Twist	0.000	-0.112	-0.157	0.000	0.012	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.264	0.000	0.000	0.024	0.000	0.000
Slide	0.000	4.232	2.867	0.000	-0.040	-0.110
Rise	0.000	2.867	10.460	0.000	0.075	-0.161
Tilt	0.024	0.000	0.000	0.039	0.000	0.000
Roll	0.000	-0.040	0.075	0.000	0.027	0.014
Twist	0.000	-0.110	-0.161	0.000	0.014	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.944	0.000	0.000	-0.008	0.000	0.000
Slide	0.000	1.080	-0.400	0.000	0.033	0.046
Rise	0.000	-0.401	4.566	0.000	0.008	-0.020
Tilt	-0.008	0.000	0.000	0.016	0.000	0.000
Roll	0.000	0.033	0.008	0.000	0.017	-0.001
Twist	0.000	0.046	-0.020	0.000	-0.001	0.003

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.370	0.000	0.000	-0.047	0.000	0.000
Slide	0.000	2.313	1.515	0.000	-0.032	-0.186
Rise	0.000	1.515	6.932	0.000	-0.033	-0.169
Tilt	-0.047	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.032	-0.033	0.000	0.020	0.011
Twist	0.000	-0.186	-0.169	0.000	0.011	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.251	0.000	0.000	-0.058	0.000	0.000
Slide	0.000	1.923	1.174	0.000	-0.036	-0.127
Rise	0.000	1.174	7.251	0.000	-0.035	-0.154
Tilt	-0.058	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.036	-0.035	0.000	0.022	0.011
Twist	0.000	-0.127	-0.154	0.000	0.011	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.144	0.000	0.000	-0.135	0.000	0.000
Slide	0.000	0.734	0.885	0.000	-0.007	0.000
Rise	0.000	0.885	6.621	0.000	-0.039	-0.151
Tilt	-0.135	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.007	-0.039	0.000	0.022	0.007
Twist	0.000	0.000	-0.151	0.000	0.007	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.322	0.000	0.000	-0.046	0.000	0.000
Slide	0.000	2.192	1.563	0.000	-0.041	-0.180
Rise	0.000	1.563	7.244	0.000	-0.042	-0.183
Tilt	-0.046	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.041	-0.042	0.000	0.020	0.011
Twist	0.000	-0.180	-0.183	0.000	0.011	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.078	0.000	0.000	-0.078	0.000	0.000
Slide	0.000	1.455	1.609	0.000	-0.023	-0.098
Rise	0.000	1.609	7.074	0.000	-0.043	-0.166
Tilt	-0.078	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.023	-0.043	0.000	0.022	0.009
Twist	0.000	-0.098	-0.166	0.000	0.009	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.907	0.000	0.000	-0.079	0.000	0.000
Slide	0.000	1.399	1.571	0.000	-0.019	-0.087
Rise	0.000	1.572	7.618	0.000	-0.045	-0.196
Tilt	-0.079	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.019	-0.045	0.000	0.020	0.009
Twist	0.000	-0.087	-0.196	0.000	0.009	0.021

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.268	0.000	0.000	-0.083	0.000	0.000
Slide	0.000	1.426	1.629	0.000	-0.021	-0.092
Rise	0.000	1.629	6.631	0.000	-0.048	-0.172
Tilt	-0.083	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.021	-0.048	0.000	0.023	0.008
Twist	0.000	-0.092	-0.172	0.000	0.008	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.901	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	1.850	0.846	0.000	0.008	-0.065
Rise	0.000	0.845	7.288	0.000	-0.113	-0.222
Tilt	-0.037	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.008	-0.113	0.000	0.019	0.009
Twist	0.000	-0.065	-0.222	0.000	0.009	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.982	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	1.805	1.148	0.000	-0.003	-0.074
Rise	0.000	1.148	6.491	0.000	-0.072	-0.193
Tilt	-0.054	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.003	-0.072	0.000	0.018	0.009
Twist	0.000	-0.074	-0.193	0.000	0.009	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.947	0.000	0.000	-0.042	0.000	0.000
Slide	0.000	1.984	0.996	0.000	0.006	-0.071
Rise	0.000	0.996	6.849	0.000	-0.102	-0.208
Tilt	-0.042	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.006	-0.102	0.000	0.019	0.009
Twist	0.000	-0.071	-0.208	0.000	0.009	0.020

[Image courtesy of Curves+]

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	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-atat.12.cte	0.000	0.000

	Mean	Stdev
-atat.4.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-gatc.11.cte	0.000	0.000

	Mean	Stdev
-gatc.15.cte	0.000	0.000

	Mean	Stdev
-gatc.3.cte	0.000	0.000

	Mean	Stdev
-gatc.7.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
-tgca.13.cte	0.000	0.000

	Mean	Stdev
-tgca.5.cte	0.000	0.000

	Mean	Stdev
-tgca.9.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.529	0.339

	Mean	Stdev
roll-10-GGCC	5.987	5.711

	Mean	Stdev
shift-10-GGCC	0.000	0.807

	Mean	Stdev
slide-10-GGCC	-0.718	0.808

	Mean	Stdev
tilt-10-GGCC	0.000	4.526

	Mean	Stdev
twist-10-GGCC	31.932	6.505

	Mean	Stdev
rise-11-GATC	3.394	0.317

	Mean	Stdev
roll-11-GATC	2.070	5.944

	Mean	Stdev
shift-11-GATC	0.000	0.674

	Mean	Stdev
slide-11-GATC	-0.550	0.671

	Mean	Stdev
tilt-11-GATC	0.000	4.658

	Mean	Stdev
twist-11-GATC	36.185	5.513

	Mean	Stdev
rise-12-ATAT	3.227	0.281

	Mean	Stdev
roll-12-ATAT	-0.661	5.464

	Mean	Stdev
shift-12-ATAT	0.000	0.677

	Mean	Stdev
slide-12-ATAT	-0.748	0.435

	Mean	Stdev
tilt-12-ATAT	0.000	3.928

	Mean	Stdev
twist-12-ATAT	29.891	4.435

	Mean	Stdev
rise-13-TGCA	3.113	0.355

	Mean	Stdev
roll-13-TGCA	9.611	6.420

	Mean	Stdev
shift-13-TGCA	0.000	0.831

	Mean	Stdev
slide-13-TGCA	-0.125	0.620

	Mean	Stdev
tilt-13-TGCA	0.000	4.917

	Mean	Stdev
twist-13-TGCA	30.669	8.034

	Mean	Stdev
rise-14-GGCC	3.429	0.337

	Mean	Stdev
roll-14-GGCC	5.269	6.044

	Mean	Stdev
shift-14-GGCC	0.000	0.899

	Mean	Stdev
slide-14-GGCC	-0.419	0.794

	Mean	Stdev
tilt-14-GGCC	0.000	4.584

	Mean	Stdev
twist-14-GGCC	32.447	7.289

	Mean	Stdev
rise-15-GATC	3.344	0.307

	Mean	Stdev
roll-15-GATC	2.222	5.764

	Mean	Stdev
shift-15-GATC	0.000	0.718

	Mean	Stdev
slide-15-GATC	-0.294	0.671

	Mean	Stdev
tilt-15-GATC	0.000	4.610

	Mean	Stdev
twist-15-GATC	35.078	6.171

	Mean	Stdev
rise-16-AGCT	3.412	0.442

	Mean	Stdev
roll-16-AGCT	3.787	5.829

	Mean	Stdev
shift-16-AGCT	0.000	0.757

	Mean	Stdev
slide-16-AGCT	-0.649	0.661

	Mean	Stdev
tilt-16-AGCT	0.000	5.803

	Mean	Stdev
twist-16-AGCT	32.845	7.029

	Mean	Stdev
rise-2-CGCG	3.242	0.365

	Mean	Stdev
roll-2-CGCG	5.586	7.100

	Mean	Stdev
shift-2-CGCG	0.000	0.790

	Mean	Stdev
slide-2-CGCG	1.394	1.447

	Mean	Stdev
tilt-2-CGCG	0.000	6.162

	Mean	Stdev
twist-2-CGCG	4.044	26.391

	Mean	Stdev
rise-3-GATC	3.229	0.365

	Mean	Stdev
roll-3-GATC	2.194	5.503

	Mean	Stdev
shift-3-GATC	0.000	1.178

	Mean	Stdev
slide-3-GATC	0.778	1.000

	Mean	Stdev
tilt-3-GATC	0.000	7.909

	Mean	Stdev
twist-3-GATC	34.054	6.392

	Mean	Stdev
rise-4-ATAT	3.290	0.271

	Mean	Stdev
roll-4-ATAT	-0.657	5.187

	Mean	Stdev
shift-4-ATAT	0.000	0.731

	Mean	Stdev
slide-4-ATAT	-0.714	0.391

	Mean	Stdev
tilt-4-ATAT	0.000	3.816

	Mean	Stdev
twist-4-ATAT	29.738	4.328

	Mean	Stdev
rise-5-TGCA	3.265	0.359

	Mean	Stdev
roll-5-TGCA	10.148	6.435

	Mean	Stdev
shift-5-TGCA	0.000	0.819

	Mean	Stdev
slide-5-TGCA	-0.237	0.638

	Mean	Stdev
tilt-5-TGCA	0.000	4.928

	Mean	Stdev
twist-5-TGCA	32.840	7.280

	Mean	Stdev
rise-6-GGCC	3.550	0.361

	Mean	Stdev
roll-6-GGCC	6.414	5.440

	Mean	Stdev
shift-6-GGCC	0.000	0.745

	Mean	Stdev
slide-6-GGCC	-0.830	0.810

	Mean	Stdev
tilt-6-GGCC	0.000	4.649

	Mean	Stdev
twist-6-GGCC	31.498	6.316

	Mean	Stdev
rise-7-GATC	3.349	0.313

	Mean	Stdev
roll-7-GATC	2.315	5.911

	Mean	Stdev
shift-7-GATC	0.000	0.686

	Mean	Stdev
slide-7-GATC	-0.520	0.687

	Mean	Stdev
tilt-7-GATC	0.000	4.743

	Mean	Stdev
twist-7-GATC	35.772	5.526

	Mean	Stdev
rise-8-ATAT	3.211	0.279

	Mean	Stdev
roll-8-ATAT	-0.317	5.440

	Mean	Stdev
shift-8-ATAT	0.000	0.685

	Mean	Stdev
slide-8-ATAT	-0.771	0.423

	Mean	Stdev
tilt-8-ATAT	0.000	3.877

	Mean	Stdev
twist-8-ATAT	29.471	4.522

	Mean	Stdev
rise-9-TGCA	3.154	0.356

	Mean	Stdev
roll-9-TGCA	9.751	6.376

	Mean	Stdev
shift-9-TGCA	0.000	0.817

	Mean	Stdev
slide-9-TGCA	-0.208	0.601

	Mean	Stdev
tilt-9-TGCA	0.000	4.910

	Mean	Stdev
twist-9-TGCA	31.316	7.453

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