NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.547	0.000	0.000	-0.147	0.000	0.000
Slide	0.000	2.285	1.649	0.000	0.002	-0.099
Rise	0.000	1.649	6.062	0.000	-0.071	-0.200
Tilt	-0.147	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.002	-0.071	0.000	0.016	0.006
Twist	0.000	-0.099	-0.200	0.000	0.006	0.023

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.990	0.000	0.000	-0.098	0.000	0.000
Slide	0.000	2.202	1.616	0.000	-0.001	-0.217
Rise	0.000	1.616	7.893	0.000	-0.038	-0.163
Tilt	-0.098	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.001	-0.038	0.000	0.022	0.008
Twist	0.000	-0.217	-0.163	0.000	0.008	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.999	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	1.872	1.509	0.000	0.008	-0.178
Rise	0.000	1.509	7.171	0.000	-0.011	-0.161
Tilt	-0.094	0.000	0.000	0.035	0.000	0.000
Roll	0.000	0.008	-0.011	0.000	0.019	0.007
Twist	0.000	-0.178	-0.161	0.000	0.007	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.989	0.000	0.000	-0.096	0.000	0.000
Slide	0.000	2.151	1.491	0.000	0.006	-0.218
Rise	0.000	1.491	7.611	0.000	-0.030	-0.144
Tilt	-0.096	0.000	0.000	0.038	0.000	0.000
Roll	0.000	0.006	-0.030	0.000	0.022	0.007
Twist	0.000	-0.218	-0.144	0.000	0.007	0.044

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.568	0.000	0.000	-0.033	0.000	0.000
Slide	0.000	4.368	2.840	0.000	0.016	-0.193
Rise	0.000	2.840	9.007	0.000	0.064	-0.182
Tilt	-0.033	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.016	0.064	0.000	0.028	0.008
Twist	0.000	-0.193	-0.182	0.000	0.008	0.051

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.209	0.000	0.000	-0.040	0.000	0.000
Slide	0.000	3.196	2.530	0.000	0.018	-0.128
Rise	0.000	2.530	8.806	0.000	0.079	-0.185
Tilt	-0.040	0.000	0.000	0.035	0.000	0.000
Roll	0.000	0.018	0.079	0.000	0.025	0.010
Twist	0.000	-0.128	-0.185	0.000	0.010	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.265	0.000	0.000	-0.058	0.000	0.000
Slide	0.000	3.941	3.033	0.000	0.016	-0.130
Rise	0.000	3.033	9.855	0.000	0.064	-0.236
Tilt	-0.058	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.016	0.064	0.000	0.027	0.012
Twist	0.000	-0.130	-0.236	0.000	0.012	0.043

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.630	0.000	0.000	-0.037	0.000	0.000
Slide	0.000	4.421	2.978	0.000	0.018	-0.212
Rise	0.000	2.978	9.529	0.000	0.056	-0.219
Tilt	-0.037	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.018	0.056	0.000	0.029	0.006
Twist	0.000	-0.212	-0.219	0.000	0.006	0.050

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.494	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	2.064	0.645	0.000	0.015	-0.024
Rise	0.000	0.645	7.611	0.000	-0.153	-0.230
Tilt	-0.002	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.015	-0.153	0.000	0.023	0.009
Twist	0.000	-0.024	-0.230	0.000	0.009	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.344	0.000	0.000	0.007	0.000	0.000
Slide	0.000	0.967	-0.010	0.000	0.004	-0.020
Rise	0.000	-0.010	3.614	0.000	-0.061	-0.117
Tilt	0.007	0.000	0.000	0.012	0.000	0.000
Roll	0.000	0.004	-0.061	0.000	0.006	0.001
Twist	0.000	-0.020	-0.117	0.000	0.001	0.011

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.554	0.000	0.000	-0.000	0.000	0.000
Slide	0.000	2.334	0.619	0.000	0.017	-0.030
Rise	0.000	0.619	8.403	0.000	-0.171	-0.247
Tilt	-0.000	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.017	-0.171	0.000	0.024	0.010
Twist	0.000	-0.030	-0.247	0.000	0.010	0.020

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.543	0.000	0.000	-0.003	0.000	0.000
Slide	0.000	2.295	0.754	0.000	0.017	-0.031
Rise	0.000	0.754	8.497	0.000	-0.171	-0.249
Tilt	-0.003	0.000	0.000	0.025	0.000	0.000
Roll	0.000	0.017	-0.171	0.000	0.025	0.009
Twist	0.000	-0.031	-0.249	0.000	0.009	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.059	0.000	0.000	-0.100	0.000	0.000
Slide	0.000	3.440	1.060	0.000	-0.054	-0.250
Rise	0.000	1.060	8.894	0.000	0.002	-0.198
Tilt	-0.100	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.054	0.002	0.000	0.022	0.011
Twist	0.000	-0.250	-0.198	0.000	0.011	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.006	0.000	0.000	-0.103	0.000	0.000
Slide	0.000	3.703	0.921	0.000	-0.059	-0.231
Rise	0.000	0.921	9.114	0.000	0.006	-0.191
Tilt	-0.103	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.059	0.006	0.000	0.022	0.012
Twist	0.000	-0.231	-0.191	0.000	0.012	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.086	0.000	0.000	-0.104	0.000	0.000
Slide	0.000	3.738	0.939	0.000	-0.062	-0.263
Rise	0.000	0.939	9.142	0.000	0.017	-0.175
Tilt	-0.104	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.062	0.017	0.000	0.022	0.012
Twist	0.000	-0.263	-0.175	0.000	0.012	0.043

[Image courtesy of Curves+]

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	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
-gtac.11.cte	0.000	0.000

	Mean	Stdev
-gtac.15.cte	0.000	0.000

	Mean	Stdev
-gtac.3.cte	0.000	0.000

	Mean	Stdev
-gtac.7.cte	0.000	0.000

	Mean	Stdev
-tgca.13.cte	0.000	0.000

	Mean	Stdev
-tgca.16.cte	0.000	0.000

	Mean	Stdev
-tgca.5.cte	0.000	0.000

	Mean	Stdev
-tgca.9.cte	0.000	0.000

	Mean	Stdev
-ttaa.12.cte	0.000	0.000

	Mean	Stdev
-ttaa.4.cte	0.000	0.000

	Mean	Stdev
-ttaa.8.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.579	0.297

	Mean	Stdev
roll-10-GGCC	4.052	5.583

	Mean	Stdev
shift-10-GGCC	0.000	0.889

	Mean	Stdev
slide-10-GGCC	-0.609	0.807

	Mean	Stdev
tilt-10-GGCC	0.000	4.524

	Mean	Stdev
twist-10-GGCC	35.333	5.851

	Mean	Stdev
rise-11-GTAC	3.458	0.291

	Mean	Stdev
roll-11-GTAC	-0.430	4.798

	Mean	Stdev
shift-11-GTAC	0.000	0.616

	Mean	Stdev
slide-11-GTAC	-0.808	0.437

	Mean	Stdev
tilt-11-GTAC	0.000	3.755

	Mean	Stdev
twist-11-GTAC	33.389	3.861

	Mean	Stdev
rise-12-TTAA	3.307	0.274

	Mean	Stdev
roll-12-TTAA	-1.030	5.619

	Mean	Stdev
shift-12-TTAA	0.000	0.896

	Mean	Stdev
slide-12-TTAA	-0.026	0.550

	Mean	Stdev
tilt-12-TTAA	0.000	5.273

	Mean	Stdev
twist-12-TTAA	37.353	5.463

	Mean	Stdev
rise-13-TGCA	3.000	0.356

	Mean	Stdev
roll-13-TGCA	11.382	5.769

	Mean	Stdev
shift-13-TGCA	0.000	0.626

	Mean	Stdev
slide-13-TGCA	-0.140	0.547

	Mean	Stdev
tilt-13-TGCA	0.000	4.823

	Mean	Stdev
twist-13-TGCA	24.008	7.327

	Mean	Stdev
rise-14-GGCC	3.553	0.314

	Mean	Stdev
roll-14-GGCC	3.758	6.006

	Mean	Stdev
shift-14-GGCC	0.000	0.885

	Mean	Stdev
slide-14-GGCC	-0.612	0.849

	Mean	Stdev
tilt-14-GGCC	0.000	4.699

	Mean	Stdev
twist-14-GGCC	35.118	6.150

	Mean	Stdev
rise-15-GTAC	3.370	0.308

	Mean	Stdev
roll-15-GTAC	-0.109	5.441

	Mean	Stdev
shift-15-GTAC	0.000	0.710

	Mean	Stdev
slide-15-GTAC	-0.676	0.507

	Mean	Stdev
tilt-15-GTAC	0.000	4.144

	Mean	Stdev
twist-15-GTAC	32.443	4.788

	Mean	Stdev
rise-16-TGCA	3.247	0.551

	Mean	Stdev
roll-16-TGCA	6.591	10.788

	Mean	Stdev
shift-16-TGCA	0.000	1.313

	Mean	Stdev
slide-16-TGCA	-0.031	0.802

	Mean	Stdev
tilt-16-TGCA	0.000	7.010

	Mean	Stdev
twist-16-TGCA	30.912	9.334

	Mean	Stdev
rise-2-CGCG	3.532	0.387

	Mean	Stdev
roll-2-CGCG	9.197	6.457

	Mean	Stdev
shift-2-CGCG	0.000	0.819

	Mean	Stdev
slide-2-CGCG	-0.183	0.595

	Mean	Stdev
tilt-2-CGCG	0.000	5.690

	Mean	Stdev
twist-2-CGCG	38.021	6.403

	Mean	Stdev
rise-3-GTAC	3.417	0.297

	Mean	Stdev
roll-3-GTAC	-0.551	5.229

	Mean	Stdev
shift-3-GTAC	0.000	0.705

	Mean	Stdev
slide-3-GTAC	-0.672	0.449

	Mean	Stdev
tilt-3-GTAC	0.000	3.929

	Mean	Stdev
twist-3-GTAC	32.312	4.462

	Mean	Stdev
rise-4-TTAA	3.283	0.270

	Mean	Stdev
roll-4-TTAA	-1.398	5.718

	Mean	Stdev
shift-4-TTAA	0.000	0.959

	Mean	Stdev
slide-4-TTAA	0.067	0.493

	Mean	Stdev
tilt-4-TTAA	0.000	5.646

	Mean	Stdev
twist-4-TTAA	37.761	5.188

	Mean	Stdev
rise-5-TGCA	2.929	0.337

	Mean	Stdev
roll-5-TGCA	11.361	5.633

	Mean	Stdev
shift-5-TGCA	0.000	0.614

	Mean	Stdev
slide-5-TGCA	-0.151	0.514

	Mean	Stdev
tilt-5-TGCA	0.000	4.741

	Mean	Stdev
twist-5-TGCA	22.784	7.155

	Mean	Stdev
rise-6-GGCC	3.592	0.300

	Mean	Stdev
roll-6-GGCC	4.051	5.569

	Mean	Stdev
shift-6-GGCC	0.000	0.885

	Mean	Stdev
slide-6-GGCC	-0.608	0.807

	Mean	Stdev
tilt-6-GGCC	0.000	4.530

	Mean	Stdev
twist-6-GGCC	35.785	5.540

	Mean	Stdev
rise-7-GTAC	3.455	0.284

	Mean	Stdev
roll-7-GTAC	-0.474	4.640

	Mean	Stdev
shift-7-GTAC	0.000	0.606

	Mean	Stdev
slide-7-GTAC	-0.810	0.441

	Mean	Stdev
tilt-7-GTAC	0.000	3.759

	Mean	Stdev
twist-7-GTAC	33.225	3.959

	Mean	Stdev
rise-8-TTAA	3.301	0.268

	Mean	Stdev
roll-8-TTAA	-1.189	5.719

	Mean	Stdev
shift-8-TTAA	0.000	0.891

	Mean	Stdev
slide-8-TTAA	0.004	0.526

	Mean	Stdev
tilt-8-TTAA	0.000	5.268

	Mean	Stdev
twist-8-TTAA	37.550	5.396

	Mean	Stdev
rise-9-TGCA	2.950	0.342

	Mean	Stdev
roll-9-TGCA	11.207	5.571

	Mean	Stdev
shift-9-TGCA	0.000	0.616

	Mean	Stdev
slide-9-TGCA	-0.137	0.521

	Mean	Stdev
tilt-9-TGCA	0.000	4.875

	Mean	Stdev
twist-9-TGCA	23.142	7.410

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