NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.879	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	2.007	1.396	0.000	-0.031	-0.114
Rise	0.000	1.396	7.210	0.000	-0.034	-0.179
Tilt	-0.095	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.031	-0.034	0.000	0.018	0.006
Twist	0.000	-0.114	-0.179	0.000	0.006	0.026

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.307	0.000	0.000	0.025	0.000	0.000
Slide	0.000	4.081	3.076	0.000	-0.060	-0.077
Rise	0.000	3.076	9.932	0.000	0.039	-0.164
Tilt	0.025	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.060	0.039	0.000	0.028	0.013
Twist	0.000	-0.077	-0.164	0.000	0.013	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.283	0.000	0.000	0.027	0.000	0.000
Slide	0.000	4.111	2.959	0.000	-0.053	-0.093
Rise	0.000	2.959	10.190	0.000	0.067	-0.179
Tilt	0.027	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.053	0.067	0.000	0.027	0.012
Twist	0.000	-0.093	-0.179	0.000	0.012	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.289	0.000	0.000	0.027	0.000	0.000
Slide	0.000	4.045	2.858	0.000	-0.047	-0.083
Rise	0.000	2.858	9.624	0.000	0.047	-0.160
Tilt	0.027	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.047	0.047	0.000	0.027	0.013
Twist	0.000	-0.083	-0.160	0.000	0.013	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.949	0.000	0.000	-0.101	0.000	0.000
Slide	0.000	2.034	1.476	0.000	-0.053	-0.112
Rise	0.000	1.476	7.355	0.000	-0.032	-0.188
Tilt	-0.101	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.053	-0.032	0.000	0.020	0.007
Twist	0.000	-0.112	-0.188	0.000	0.007	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.089	0.000	0.000	-0.025	0.000	0.000
Slide	0.000	3.049	2.649	0.000	0.012	-0.081
Rise	0.000	2.649	9.403	0.000	0.068	-0.211
Tilt	-0.025	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.012	0.068	0.000	0.023	0.010
Twist	0.000	-0.081	-0.211	0.000	0.010	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.918	0.000	0.000	-0.011	0.000	0.000
Slide	0.000	3.257	2.726	0.000	0.023	-0.083
Rise	0.000	2.726	9.938	0.000	0.092	-0.215
Tilt	-0.011	0.000	0.000	0.036	0.000	0.000
Roll	0.000	0.023	0.092	0.000	0.023	0.011
Twist	0.000	-0.083	-0.215	0.000	0.011	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.088	0.000	0.000	-0.024	0.000	0.000
Slide	0.000	3.217	2.888	0.000	0.012	-0.085
Rise	0.000	2.888	9.906	0.000	0.085	-0.220
Tilt	-0.024	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.012	0.085	0.000	0.022	0.008
Twist	0.000	-0.085	-0.220	0.000	0.008	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.792	0.000	0.000	-0.010	0.000	0.000
Slide	0.000	1.808	1.045	0.000	0.008	-0.098
Rise	0.000	1.045	6.536	0.000	-0.094	-0.210
Tilt	-0.010	0.000	0.000	0.022	0.000	0.000
Roll	0.000	0.008	-0.094	0.000	0.016	0.011
Twist	0.000	-0.098	-0.210	0.000	0.011	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.585	0.000	0.000	-0.019	0.000	0.000
Slide	0.000	1.549	0.716	0.000	0.015	-0.069
Rise	0.000	0.716	7.102	0.000	-0.117	-0.224
Tilt	-0.019	0.000	0.000	0.023	0.000	0.000
Roll	0.000	0.015	-0.117	0.000	0.019	0.012
Twist	0.000	-0.069	-0.224	0.000	0.012	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.691	0.000	0.000	-0.022	0.000	0.000
Slide	0.000	1.444	0.893	0.000	0.009	-0.070
Rise	0.000	0.893	6.284	0.000	-0.092	-0.214
Tilt	-0.022	0.000	0.000	0.023	0.000	0.000
Roll	0.000	0.009	-0.092	0.000	0.017	0.012
Twist	0.000	-0.070	-0.214	0.000	0.012	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.737	0.000	0.000	-0.015	0.000	0.000
Slide	0.000	1.696	0.963	0.000	0.010	-0.093
Rise	0.000	0.963	6.282	0.000	-0.085	-0.197
Tilt	-0.015	0.000	0.000	0.022	0.000	0.000
Roll	0.000	0.010	-0.085	0.000	0.016	0.011
Twist	0.000	-0.093	-0.197	0.000	0.011	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.935	0.000	0.000	-0.049	0.000	0.000
Slide	0.000	1.750	1.224	0.000	-0.003	-0.088
Rise	0.000	1.224	5.613	0.000	-0.071	-0.170
Tilt	-0.049	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.003	-0.071	0.000	0.016	0.008
Twist	0.000	-0.088	-0.170	0.000	0.008	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.019	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	1.916	1.291	0.000	-0.008	-0.108
Rise	0.000	1.291	5.243	0.000	-0.072	-0.171
Tilt	-0.053	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.008	-0.072	0.000	0.016	0.009
Twist	0.000	-0.108	-0.172	0.000	0.009	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.993	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	2.117	1.447	0.000	-0.008	-0.127
Rise	0.000	1.447	5.227	0.000	-0.067	-0.184
Tilt	-0.054	0.000	0.000	0.025	0.000	0.000
Roll	0.000	-0.008	-0.067	0.000	0.015	0.008
Twist	0.000	-0.127	-0.184	0.000	0.008	0.028

[Image courtesy of Curves+]

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	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-atat.12.cte	0.000	0.000

	Mean	Stdev
-atat.4.cte	0.000	0.000

	Mean	Stdev
-atat.8.cte	0.000	0.000

	Mean	Stdev
-ctag.2.cte	0.000	0.000

	Mean	Stdev
-gtac.10.cte	0.000	0.000

	Mean	Stdev
-gtac.14.cte	0.000	0.000

	Mean	Stdev
-gtac.6.cte	0.000	0.000

	Mean	Stdev
-tata.11.cte	0.000	0.000

	Mean	Stdev
-tata.15.cte	0.000	0.000

	Mean	Stdev
-tata.3.cte	0.000	0.000

	Mean	Stdev
-tata.7.cte	0.000	0.000

	Mean	Stdev
-tgca.13.cte	0.000	0.000

	Mean	Stdev
-tgca.5.cte	0.000	0.000

	Mean	Stdev
-tgca.9.cte	0.000	0.000

	Mean	Stdev
rise-10-GTAC	3.272	0.319

	Mean	Stdev
roll-10-GTAC	0.535	5.940

	Mean	Stdev
shift-10-GTAC	0.000	0.740

	Mean	Stdev
slide-10-GTAC	-0.646	0.507

	Mean	Stdev
tilt-10-GTAC	0.000	4.061

	Mean	Stdev
twist-10-GTAC	30.524	5.485

	Mean	Stdev
rise-11-TATA	3.305	0.348

	Mean	Stdev
roll-11-TATA	8.561	7.779

	Mean	Stdev
shift-11-TATA	0.000	0.863

	Mean	Stdev
slide-11-TATA	-0.356	0.691

	Mean	Stdev
tilt-11-TATA	0.000	5.149

	Mean	Stdev
twist-11-TATA	33.458	6.903

	Mean	Stdev
rise-12-ATAT	3.219	0.296

	Mean	Stdev
roll-12-ATAT	0.464	5.227

	Mean	Stdev
shift-12-ATAT	0.000	0.674

	Mean	Stdev
slide-12-ATAT	-0.785	0.447

	Mean	Stdev
tilt-12-ATAT	0.000	3.942

	Mean	Stdev
twist-12-ATAT	29.097	4.688

	Mean	Stdev
rise-13-TGCA	3.434	0.377

	Mean	Stdev
roll-13-TGCA	8.560	7.005

	Mean	Stdev
shift-13-TGCA	0.000	0.836

	Mean	Stdev
slide-13-TGCA	-0.418	0.677

	Mean	Stdev
tilt-13-TGCA	0.000	5.096

	Mean	Stdev
twist-13-TGCA	35.852	6.398

	Mean	Stdev
rise-14-GTAC	3.236	0.314

	Mean	Stdev
roll-14-GTAC	0.205	6.120

	Mean	Stdev
shift-14-GTAC	0.000	0.801

	Mean	Stdev
slide-14-GTAC	-0.567	0.486

	Mean	Stdev
tilt-14-GTAC	0.000	4.052

	Mean	Stdev
twist-14-GTAC	30.095	5.683

	Mean	Stdev
rise-15-TATA	3.171	0.344

	Mean	Stdev
roll-15-TATA	8.599	7.454

	Mean	Stdev
shift-15-TATA	0.000	1.014

	Mean	Stdev
slide-15-TATA	-0.140	0.719

	Mean	Stdev
tilt-15-TATA	0.000	5.108

	Mean	Stdev
twist-15-TATA	31.008	7.897

	Mean	Stdev
rise-16-AGCT	3.360	0.320

	Mean	Stdev
roll-16-AGCT	2.957	5.880

	Mean	Stdev
shift-16-AGCT	0.000	1.024

	Mean	Stdev
slide-16-AGCT	-0.419	0.638

	Mean	Stdev
tilt-16-AGCT	0.000	5.723

	Mean	Stdev
twist-16-AGCT	34.703	5.878

	Mean	Stdev
rise-2-CTAG	3.359	0.321

	Mean	Stdev
roll-2-CTAG	3.165	5.846

	Mean	Stdev
shift-2-CTAG	0.000	0.981

	Mean	Stdev
slide-2-CTAG	-0.508	0.636

	Mean	Stdev
tilt-2-CTAG	0.000	5.523

	Mean	Stdev
twist-2-CTAG	34.264	5.801

	Mean	Stdev
rise-3-TATA	3.305	0.365

	Mean	Stdev
roll-3-TATA	9.425	7.713

	Mean	Stdev
shift-3-TATA	0.000	0.935

	Mean	Stdev
slide-3-TATA	-0.315	0.738

	Mean	Stdev
tilt-3-TATA	0.000	5.080

	Mean	Stdev
twist-3-TATA	32.215	7.352

	Mean	Stdev
rise-4-ATAT	3.200	0.291

	Mean	Stdev
roll-4-ATAT	0.197	5.280

	Mean	Stdev
shift-4-ATAT	0.000	0.681

	Mean	Stdev
slide-4-ATAT	-0.776	0.438

	Mean	Stdev
tilt-4-ATAT	0.000	3.987

	Mean	Stdev
twist-4-ATAT	29.211	4.376

	Mean	Stdev
rise-5-TGCA	3.471	0.391

	Mean	Stdev
roll-5-TGCA	8.706	6.839

	Mean	Stdev
shift-5-TGCA	0.000	0.805

	Mean	Stdev
slide-5-TGCA	-0.485	0.664

	Mean	Stdev
tilt-5-TGCA	0.000	5.180

	Mean	Stdev
twist-5-TGCA	35.926	6.208

	Mean	Stdev
rise-6-GTAC	3.266	0.318

	Mean	Stdev
roll-6-GTAC	0.561	5.817

	Mean	Stdev
shift-6-GTAC	0.000	0.740

	Mean	Stdev
slide-6-GTAC	-0.655	0.499

	Mean	Stdev
tilt-6-GTAC	0.000	4.037

	Mean	Stdev
twist-6-GTAC	30.050	5.506

	Mean	Stdev
rise-7-TATA	3.279	0.350

	Mean	Stdev
roll-7-TATA	8.134	7.811

	Mean	Stdev
shift-7-TATA	0.000	0.898

	Mean	Stdev
slide-7-TATA	-0.286	0.716

	Mean	Stdev
tilt-7-TATA	0.000	5.184

	Mean	Stdev
twist-7-TATA	33.599	6.988

	Mean	Stdev
rise-8-ATAT	3.222	0.295

	Mean	Stdev
roll-8-ATAT	0.301	5.334

	Mean	Stdev
shift-8-ATAT	0.000	0.680

	Mean	Stdev
slide-8-ATAT	-0.776	0.438

	Mean	Stdev
tilt-8-ATAT	0.000	3.998

	Mean	Stdev
twist-8-ATAT	29.226	4.605

	Mean	Stdev
rise-9-TGCA	3.481	0.396

	Mean	Stdev
roll-9-TGCA	8.799	6.963

	Mean	Stdev
shift-9-TGCA	0.000	0.818

	Mean	Stdev
slide-9-TGCA	-0.474	0.653

	Mean	Stdev
tilt-9-TGCA	0.000	5.127

	Mean	Stdev
twist-9-TGCA	35.891	6.124

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