NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.402	0.000	0.000	0.033	0.000	0.000
Slide	0.000	4.571	0.755	0.000	0.010	-0.239
Rise	0.000	0.755	7.919	0.000	-0.197	-0.203
Tilt	0.033	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.010	-0.197	0.000	0.020	0.004
Twist	0.000	-0.239	-0.203	0.000	0.004	0.068

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.258	0.000	0.000	0.031	0.000	0.000
Slide	0.000	4.511	1.075	0.000	0.019	-0.241
Rise	0.000	1.075	7.734	0.000	-0.190	-0.201
Tilt	0.031	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.019	-0.190	0.000	0.021	0.004
Twist	0.000	-0.241	-0.201	0.000	0.004	0.067

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.083	0.000	0.000	-0.009	0.000	0.000
Slide	0.000	3.578	2.307	0.000	-0.033	-0.260
Rise	0.000	2.307	12.970	0.000	-0.172	-0.232
Tilt	-0.009	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.033	-0.172	0.000	0.026	0.003
Twist	0.000	-0.260	-0.232	0.000	0.003	0.060

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.940	0.000	0.000	0.005	0.000	0.000
Slide	0.000	3.888	3.018	0.000	-0.082	-0.135
Rise	0.000	3.018	11.974	0.000	-0.251	-0.204
Tilt	0.005	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.082	-0.251	0.000	0.024	0.006
Twist	0.000	-0.135	-0.204	0.000	0.006	0.050

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.002	0.000	0.000	0.010	0.000	0.000
Slide	0.000	3.971	2.992	0.000	-0.079	-0.165
Rise	0.000	2.992	12.428	0.000	-0.228	-0.175
Tilt	0.010	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.079	-0.228	0.000	0.023	0.006
Twist	0.000	-0.165	-0.175	0.000	0.006	0.056

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.369	0.000	0.000	-0.080	0.000	0.000
Slide	0.000	6.653	1.354	0.000	0.002	-0.281
Rise	0.000	1.354	8.901	0.000	-0.126	-0.252
Tilt	-0.080	0.000	0.000	0.046	0.000	0.000
Roll	0.000	0.002	-0.126	0.000	0.027	0.000
Twist	0.000	-0.281	-0.252	0.000	0.000	0.072

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.355	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	6.652	1.400	0.000	-0.001	-0.268
Rise	0.000	1.400	8.630	0.000	-0.141	-0.253
Tilt	-0.084	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.001	-0.141	0.000	0.027	0.001
Twist	0.000	-0.268	-0.253	0.000	0.001	0.070

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.047	0.000	0.000	-0.086	0.000	0.000
Slide	0.000	5.014	1.685	0.000	-0.046	-0.284
Rise	0.000	1.685	4.200	0.000	-0.037	-0.082
Tilt	-0.086	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.046	-0.037	0.000	0.016	-0.006
Twist	0.000	-0.284	-0.082	0.000	-0.006	0.060

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.363	0.000	0.000	-0.033	0.000	0.000
Slide	0.000	5.750	1.374	0.000	-0.008	-0.269
Rise	0.000	1.374	8.782	0.000	-0.132	-0.184
Tilt	-0.033	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.008	-0.132	0.000	0.021	-0.002
Twist	0.000	-0.269	-0.184	0.000	-0.002	0.072

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.318	0.000	0.000	-0.030	0.000	0.000
Slide	0.000	6.089	1.400	0.000	-0.020	-0.303
Rise	0.000	1.400	8.817	0.000	-0.140	-0.176
Tilt	-0.030	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.020	-0.140	0.000	0.021	-0.001
Twist	0.000	-0.303	-0.176	0.000	-0.001	0.075

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.218	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	4.835	2.712	0.000	-0.026	-0.316
Rise	0.000	2.713	16.903	0.000	-0.059	-0.264
Tilt	-0.054	0.000	0.000	0.050	0.000	0.000
Roll	0.000	-0.026	-0.059	0.000	0.033	0.001
Twist	0.000	-0.316	-0.264	0.000	0.001	0.071

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.941	0.000	0.000	0.041	0.000	0.000
Slide	0.000	5.896	0.492	0.000	-0.066	-0.195
Rise	0.000	0.492	5.683	0.000	-0.101	-0.076
Tilt	0.041	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.066	-0.101	0.000	0.015	-0.000
Twist	0.000	-0.195	-0.076	0.000	-0.000	0.056

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.028	0.000	0.000	0.058	0.000	0.000
Slide	0.000	4.833	0.416	0.000	-0.030	-0.051
Rise	0.000	0.416	5.517	0.000	-0.148	-0.157
Tilt	0.058	0.000	0.000	0.024	0.000	0.000
Roll	0.000	-0.030	-0.148	0.000	0.016	0.002
Twist	0.000	-0.051	-0.157	0.000	0.002	0.058

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.962	0.000	0.000	0.056	0.000	0.000
Slide	0.000	5.903	0.462	0.000	-0.072	-0.169
Rise	0.000	0.462	6.167	0.000	-0.109	-0.117
Tilt	0.056	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.072	-0.109	0.000	0.015	0.001
Twist	0.000	-0.169	-0.117	0.000	0.001	0.072

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.059	0.000	0.000	0.049	0.000	0.000
Slide	0.000	5.734	0.392	0.000	-0.054	-0.151
Rise	0.000	0.392	5.184	0.000	-0.103	-0.100
Tilt	0.049	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.054	-0.103	0.000	0.015	-0.000
Twist	0.000	-0.151	-0.100	0.000	-0.000	0.056

[Image courtesy of Curves+]

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	Mean	Stdev
-aauu.7.cte	0.000	0.000

	Mean	Stdev
-aauu.8.cte	0.000	0.000

	Mean	Stdev
-acgu.9.cte	0.000	0.000

	Mean	Stdev
-auau.15.cte	0.000	0.000

	Mean	Stdev
-auau.5.cte	0.000	0.000

	Mean	Stdev
-ccgg.12.cte	0.000	0.000

	Mean	Stdev
-ccgg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.10.cte	0.000	0.000

	Mean	Stdev
-cuag.13.cte	0.000	0.000

	Mean	Stdev
-cuag.3.cte	0.000	0.000

	Mean	Stdev
-gcgc.11.cte	0.000	0.000

	Mean	Stdev
-uaua.14.cte	0.000	0.000

	Mean	Stdev
-uaua.16.cte	0.000	0.000

	Mean	Stdev
-uaua.4.cte	0.000	0.000

	Mean	Stdev
-uaua.6.cte	0.000	0.000

	Mean	Stdev
rise-10-CGCG	3.564	0.403

	Mean	Stdev
roll-10-CGCG	8.095	6.640

	Mean	Stdev
shift-10-CGCG	0.000	0.835

	Mean	Stdev
slide-10-CGCG	-1.694	0.441

	Mean	Stdev
tilt-10-CGCG	0.000	4.533

	Mean	Stdev
twist-10-CGCG	31.695	3.868

	Mean	Stdev
rise-11-GCGC	3.225	0.197

	Mean	Stdev
roll-11-GCGC	3.048	4.261

	Mean	Stdev
shift-11-GCGC	0.000	0.521

	Mean	Stdev
slide-11-GCGC	-1.982	0.424

	Mean	Stdev
tilt-11-GCGC	0.000	3.486

	Mean	Stdev
twist-11-GCGC	28.247	3.444

	Mean	Stdev
rise-12-CCGG	3.329	0.281

	Mean	Stdev
roll-12-CCGG	7.368	4.808

	Mean	Stdev
shift-12-CCGG	0.000	0.513

	Mean	Stdev
slide-12-CCGG	-1.906	0.325

	Mean	Stdev
tilt-12-CCGG	0.000	3.699

	Mean	Stdev
twist-12-CCGG	30.240	3.245

	Mean	Stdev
rise-13-CUAG	3.312	0.282

	Mean	Stdev
roll-13-CUAG	8.152	5.557

	Mean	Stdev
shift-13-CUAG	0.000	0.663

	Mean	Stdev
slide-13-CUAG	-1.734	0.354

	Mean	Stdev
tilt-13-CUAG	0.000	4.069

	Mean	Stdev
twist-13-CUAG	29.027	3.206

	Mean	Stdev
rise-14-UAUA	3.233	0.347

	Mean	Stdev
roll-14-UAUA	12.737	6.863

	Mean	Stdev
shift-14-UAUA	0.000	0.559

	Mean	Stdev
slide-14-UAUA	-1.370	0.346

	Mean	Stdev
tilt-14-UAUA	0.000	4.662

	Mean	Stdev
twist-14-UAUA	31.675	3.512

	Mean	Stdev
rise-15-AUAU	3.223	0.272

	Mean	Stdev
roll-15-AUAU	9.009	5.635

	Mean	Stdev
shift-15-AUAU	0.000	0.790

	Mean	Stdev
slide-15-AUAU	-1.500	0.445

	Mean	Stdev
tilt-15-AUAU	0.000	4.292

	Mean	Stdev
twist-15-AUAU	28.311	3.633

	Mean	Stdev
rise-16-UAUA	3.300	0.392

	Mean	Stdev
roll-16-UAUA	15.848	7.072

	Mean	Stdev
shift-16-UAUA	0.000	0.557

	Mean	Stdev
slide-16-UAUA	-1.219	0.352

	Mean	Stdev
tilt-16-UAUA	0.000	5.071

	Mean	Stdev
twist-16-UAUA	32.018	3.324

	Mean	Stdev
rise-2-CCGG	3.425	0.290

	Mean	Stdev
roll-2-CCGG	9.229	4.924

	Mean	Stdev
shift-2-CCGG	0.000	0.517

	Mean	Stdev
slide-2-CCGG	-1.884	0.324

	Mean	Stdev
tilt-2-CCGG	0.000	3.761

	Mean	Stdev
twist-2-CCGG	30.800	3.280

	Mean	Stdev
rise-3-CUAG	3.318	0.282

	Mean	Stdev
roll-3-CUAG	8.583	5.602

	Mean	Stdev
shift-3-CUAG	0.000	0.673

	Mean	Stdev
slide-3-CUAG	-1.741	0.349

	Mean	Stdev
tilt-3-CUAG	0.000	4.076

	Mean	Stdev
twist-3-CUAG	28.960	3.172

	Mean	Stdev
rise-4-UAUA	3.224	0.336

	Mean	Stdev
roll-4-UAUA	12.512	6.795

	Mean	Stdev
shift-4-UAUA	0.000	0.563

	Mean	Stdev
slide-4-UAUA	-1.366	0.337

	Mean	Stdev
tilt-4-UAUA	0.000	4.655

	Mean	Stdev
twist-4-UAUA	31.796	3.011

	Mean	Stdev
rise-5-AUAU	3.220	0.258

	Mean	Stdev
roll-5-AUAU	7.962	5.595

	Mean	Stdev
shift-5-AUAU	0.000	0.766

	Mean	Stdev
slide-5-AUAU	-1.553	0.446

	Mean	Stdev
tilt-5-AUAU	0.000	4.259

	Mean	Stdev
twist-5-AUAU	28.214	3.463

	Mean	Stdev
rise-6-UAUA	3.340	0.370

	Mean	Stdev
roll-6-UAUA	14.071	6.953

	Mean	Stdev
shift-6-UAUA	0.000	0.546

	Mean	Stdev
slide-6-UAUA	-1.333	0.339

	Mean	Stdev
tilt-6-UAUA	0.000	4.829

	Mean	Stdev
twist-6-UAUA	32.134	3.447

	Mean	Stdev
rise-7-AAUU	3.219	0.325

	Mean	Stdev
roll-7-AAUU	7.555	6.246

	Mean	Stdev
shift-7-AAUU	0.000	0.656

	Mean	Stdev
slide-7-AAUU	-1.551	0.398

	Mean	Stdev
tilt-7-AAUU	0.000	4.677

	Mean	Stdev
twist-7-AAUU	28.687	3.339

	Mean	Stdev
rise-8-AAUU	3.288	0.328

	Mean	Stdev
roll-8-AAUU	8.105	6.142

	Mean	Stdev
shift-8-AAUU	0.000	0.692

	Mean	Stdev
slide-8-AAUU	-1.622	0.408

	Mean	Stdev
tilt-8-AAUU	0.000	4.659

	Mean	Stdev
twist-8-AAUU	28.956	3.374

	Mean	Stdev
rise-9-ACGU	3.294	0.236

	Mean	Stdev
roll-9-ACGU	6.047	5.016

	Mean	Stdev
shift-9-ACGU	0.000	0.736

	Mean	Stdev
slide-9-ACGU	-1.944	0.504

	Mean	Stdev
tilt-9-ACGU	0.000	4.114

	Mean	Stdev
twist-9-ACGU	27.510	3.806

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