NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.035	0.000	0.000	0.004	0.000	0.000
Slide	0.000	3.610	2.665	0.000	-0.036	-0.244
Rise	0.000	2.665	13.861	0.000	-0.184	-0.293
Tilt	0.004	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.036	-0.184	0.000	0.026	0.004
Twist	0.000	-0.244	-0.293	0.000	0.004	0.065

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.306	0.000	0.000	-0.034	0.000	0.000
Slide	0.000	5.689	1.193	0.000	-0.002	-0.270
Rise	0.000	1.193	8.333	0.000	-0.126	-0.174
Tilt	-0.034	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.002	-0.126	0.000	0.021	-0.001
Twist	0.000	-0.270	-0.174	0.000	-0.001	0.072

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.832	0.000	0.000	-0.012	0.000	0.000
Slide	0.000	3.641	2.661	0.000	-0.079	-0.189
Rise	0.000	2.661	11.151	0.000	-0.246	-0.178
Tilt	-0.012	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.079	-0.246	0.000	0.022	0.004
Twist	0.000	-0.189	-0.178	0.000	0.004	0.057

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.909	0.000	0.000	0.009	0.000	0.000
Slide	0.000	4.027	3.132	0.000	-0.071	-0.259
Rise	0.000	3.132	13.177	0.000	-0.210	-0.220
Tilt	0.009	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.071	-0.210	0.000	0.022	0.005
Twist	0.000	-0.259	-0.220	0.000	0.005	0.069

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.373	0.000	0.000	-0.002	0.000	0.000
Slide	0.000	5.824	0.914	0.000	-0.067	-0.163
Rise	0.000	0.914	5.267	0.000	-0.112	-0.106
Tilt	-0.002	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.067	-0.112	0.000	0.017	-0.001
Twist	0.000	-0.163	-0.106	0.000	-0.001	0.068

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.295	0.000	0.000	-0.015	0.000	0.000
Slide	0.000	5.399	1.157	0.000	0.001	-0.302
Rise	0.000	1.157	8.355	0.000	-0.148	-0.221
Tilt	-0.015	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.001	-0.148	0.000	0.021	0.001
Twist	0.000	-0.302	-0.221	0.000	0.001	0.071

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.206	0.000	0.000	0.025	0.000	0.000
Slide	0.000	4.871	1.287	0.000	0.026	-0.251
Rise	0.000	1.287	7.549	0.000	-0.170	-0.218
Tilt	0.025	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.026	-0.170	0.000	0.022	0.005
Twist	0.000	-0.251	-0.218	0.000	0.005	0.073

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.402	0.000	0.000	0.014	0.000	0.000
Slide	0.000	4.901	1.188	0.000	0.024	-0.262
Rise	0.000	1.188	7.854	0.000	-0.192	-0.246
Tilt	0.014	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.024	-0.192	0.000	0.023	0.005
Twist	0.000	-0.262	-0.246	0.000	0.005	0.075

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.354	0.000	0.000	-0.083	0.000	0.000
Slide	0.000	6.693	1.111	0.000	0.005	-0.276
Rise	0.000	1.111	8.290	0.000	-0.143	-0.212
Tilt	-0.083	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.005	-0.143	0.000	0.026	0.001
Twist	0.000	-0.276	-0.212	0.000	0.001	0.071

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.451	0.000	0.000	-0.062	0.000	0.000
Slide	0.000	6.559	1.249	0.000	-0.005	-0.271
Rise	0.000	1.249	8.726	0.000	-0.149	-0.262
Tilt	-0.062	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.005	-0.149	0.000	0.027	0.001
Twist	0.000	-0.271	-0.262	0.000	0.001	0.070

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.066	0.000	0.000	-0.005	0.000	0.000
Slide	0.000	3.423	2.206	0.000	-0.016	-0.135
Rise	0.000	2.206	13.969	0.000	-0.100	-0.155
Tilt	-0.005	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.016	-0.100	0.000	0.025	-0.001
Twist	0.000	-0.135	-0.155	0.000	-0.001	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.009	0.000	0.000	0.069	0.000	0.000
Slide	0.000	5.692	0.417	0.000	-0.051	-0.144
Rise	0.000	0.417	6.620	0.000	-0.121	-0.133
Tilt	0.069	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.051	-0.121	0.000	0.016	0.001
Twist	0.000	-0.144	-0.133	0.000	0.001	0.065

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.338	0.000	0.000	0.027	0.000	0.000
Slide	0.000	5.345	1.296	0.000	0.029	-0.266
Rise	0.000	1.296	8.951	0.000	-0.187	-0.205
Tilt	0.027	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.029	-0.187	0.000	0.023	0.005
Twist	0.000	-0.266	-0.205	0.000	0.005	0.078

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.411	0.000	0.000	0.000	0.000	0.000
Slide	0.000	5.447	0.906	0.000	-0.054	-0.177
Rise	0.000	0.906	5.642	0.000	-0.127	-0.130
Tilt	0.000	0.000	0.000	0.032	0.000	0.000
Roll	0.000	-0.054	-0.127	0.000	0.018	-0.002
Twist	0.000	-0.177	-0.130	0.000	-0.002	0.070

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.259	0.000	0.000	-0.003	0.000	0.000
Slide	0.000	5.629	0.885	0.000	-0.069	-0.235
Rise	0.000	0.885	5.011	0.000	-0.048	-0.050
Tilt	-0.003	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.069	-0.048	0.000	0.018	-0.001
Twist	0.000	-0.235	-0.050	0.000	-0.001	0.034

[Image courtesy of Curves+]

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	Mean	Stdev
-acgu.12.cte	0.000	0.000

	Mean	Stdev
-agcu.3.cte	0.000	0.000

	Mean	Stdev
-auau.16.cte	0.000	0.000

	Mean	Stdev
-auau.9.cte	0.000	0.000

	Mean	Stdev
-caug.15.cte	0.000	0.000

	Mean	Stdev
-cuag.13.cte	0.000	0.000

	Mean	Stdev
-gauc.11.cte	0.000	0.000

	Mean	Stdev
-gauc.8.cte	0.000	0.000

	Mean	Stdev
-ggcc.4.cte	0.000	0.000

	Mean	Stdev
-ggcc.7.cte	0.000	0.000

	Mean	Stdev
-guac.5.cte	0.000	0.000

	Mean	Stdev
-uaua.2.cte	0.000	0.000

	Mean	Stdev
-ucga.14.cte	0.000	0.000

	Mean	Stdev
-ugca.10.cte	0.000	0.000

	Mean	Stdev
-ugca.6.cte	0.000	0.000

	Mean	Stdev
rise-10-UGCA	3.328	0.364

	Mean	Stdev
roll-10-UGCA	11.638	6.482

	Mean	Stdev
shift-10-UGCA	0.000	0.645

	Mean	Stdev
slide-10-UGCA	-1.360	0.350

	Mean	Stdev
tilt-10-UGCA	0.000	4.296

	Mean	Stdev
twist-10-UGCA	32.228	3.131

	Mean	Stdev
rise-11-GAUC	3.151	0.324

	Mean	Stdev
roll-11-GAUC	5.858	5.866

	Mean	Stdev
shift-11-GAUC	0.000	0.703

	Mean	Stdev
slide-11-GAUC	-1.591	0.393

	Mean	Stdev
tilt-11-GAUC	0.000	4.334

	Mean	Stdev
twist-11-GAUC	29.569	3.232

	Mean	Stdev
rise-12-ACGU	3.263	0.234

	Mean	Stdev
roll-12-ACGU	5.489	5.038

	Mean	Stdev
shift-12-ACGU	0.000	0.753

	Mean	Stdev
slide-12-ACGU	-1.826	0.484

	Mean	Stdev
tilt-12-ACGU	0.000	4.002

	Mean	Stdev
twist-12-ACGU	28.626	3.528

	Mean	Stdev
rise-13-CUAG	3.308	0.297

	Mean	Stdev
roll-13-CUAG	7.932	5.629

	Mean	Stdev
shift-13-CUAG	0.000	0.675

	Mean	Stdev
slide-13-CUAG	-1.750	0.378

	Mean	Stdev
tilt-13-CUAG	0.000	4.284

	Mean	Stdev
twist-13-CUAG	29.458	3.405

	Mean	Stdev
rise-14-UCGA	3.119	0.292

	Mean	Stdev
roll-14-UCGA	5.622	5.627

	Mean	Stdev
shift-14-UCGA	0.000	0.667

	Mean	Stdev
slide-14-UCGA	-1.541	0.372

	Mean	Stdev
tilt-14-UCGA	0.000	4.233

	Mean	Stdev
twist-14-UCGA	29.548	3.071

	Mean	Stdev
rise-15-CAUG	3.458	0.368

	Mean	Stdev
roll-15-CAUG	14.228	6.519

	Mean	Stdev
shift-15-CAUG	0.000	0.653

	Mean	Stdev
slide-15-CAUG	-1.326	0.338

	Mean	Stdev
tilt-15-CAUG	0.000	4.259

	Mean	Stdev
twist-15-CAUG	32.782	3.091

	Mean	Stdev
rise-16-AUAU	3.288	0.282

	Mean	Stdev
roll-16-AUAU	9.845	6.031

	Mean	Stdev
shift-16-AUAU	0.000	0.842

	Mean	Stdev
slide-16-AUAU	-1.548	0.476

	Mean	Stdev
tilt-16-AUAU	0.000	4.491

	Mean	Stdev
twist-16-AUAU	28.454	3.536

	Mean	Stdev
rise-2-UAUA	3.176	0.327

	Mean	Stdev
roll-2-UAUA	12.280	6.523

	Mean	Stdev
shift-2-UAUA	0.000	0.562

	Mean	Stdev
slide-2-UAUA	-1.315	0.335

	Mean	Stdev
tilt-2-UAUA	0.000	4.491

	Mean	Stdev
twist-2-UAUA	31.852	3.154

	Mean	Stdev
rise-3-AGCU	3.342	0.288

	Mean	Stdev
roll-3-AGCU	9.218	5.637

	Mean	Stdev
shift-3-AGCU	0.000	0.679

	Mean	Stdev
slide-3-AGCU	-1.713	0.356

	Mean	Stdev
tilt-3-AGCU	0.000	4.111

	Mean	Stdev
twist-3-AGCU	29.164	3.213

	Mean	Stdev
rise-4-GGCC	3.473	0.292

	Mean	Stdev
roll-4-GGCC	9.467	4.967

	Mean	Stdev
shift-4-GGCC	0.000	0.517

	Mean	Stdev
slide-4-GGCC	-1.891	0.324

	Mean	Stdev
tilt-4-GGCC	0.000	3.850

	Mean	Stdev
twist-4-GGCC	30.860	3.246

	Mean	Stdev
rise-5-GUAC	3.256	0.221

	Mean	Stdev
roll-5-GUAC	5.051	4.883

	Mean	Stdev
shift-5-GUAC	0.000	0.742

	Mean	Stdev
slide-5-GUAC	-1.892	0.472

	Mean	Stdev
tilt-5-GUAC	0.000	3.921

	Mean	Stdev
twist-5-GUAC	28.719	4.997

	Mean	Stdev
rise-6-UGCA	3.335	0.350

	Mean	Stdev
roll-6-UGCA	8.066	6.006

	Mean	Stdev
shift-6-UGCA	0.000	0.682

	Mean	Stdev
slide-6-UGCA	-1.533	0.399

	Mean	Stdev
tilt-6-UGCA	0.000	4.449

	Mean	Stdev
twist-6-UGCA	30.636	4.985

	Mean	Stdev
rise-7-GGCC	3.357	0.291

	Mean	Stdev
roll-7-GGCC	8.269	4.936

	Mean	Stdev
shift-7-GGCC	0.000	0.499

	Mean	Stdev
slide-7-GGCC	-1.799	0.326

	Mean	Stdev
tilt-7-GGCC	0.000	3.875

	Mean	Stdev
twist-7-GGCC	29.769	3.317

	Mean	Stdev
rise-8-GAUC	3.252	0.325

	Mean	Stdev
roll-8-GAUC	7.274	5.769

	Mean	Stdev
shift-8-GAUC	0.000	0.648

	Mean	Stdev
slide-8-GAUC	-1.591	0.391

	Mean	Stdev
tilt-8-GAUC	0.000	4.253

	Mean	Stdev
twist-8-GAUC	30.262	3.224

	Mean	Stdev
rise-9-AUAU	3.203	0.247

	Mean	Stdev
roll-9-AUAU	6.884	5.563

	Mean	Stdev
shift-9-AUAU	0.000	0.804

	Mean	Stdev
slide-9-AUAU	-1.617	0.473

	Mean	Stdev
tilt-9-AUAU	0.000	4.300

	Mean	Stdev
twist-9-AUAU	27.973	3.347

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