NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.342	0.000	0.000	0.041	0.000	0.000
Slide	0.000	5.117	0.990	0.000	0.025	-0.262
Rise	0.000	0.990	7.705	0.000	-0.166	-0.169
Tilt	0.041	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.025	-0.166	0.000	0.021	0.003
Twist	0.000	-0.262	-0.169	0.000	0.003	0.067

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.182	0.000	0.000	-0.009	0.000	0.000
Slide	0.000	3.552	2.505	0.000	-0.064	-0.241
Rise	0.000	2.505	12.831	0.000	-0.205	-0.239
Tilt	-0.009	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.064	-0.205	0.000	0.026	0.005
Twist	0.000	-0.241	-0.239	0.000	0.005	0.063

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.168	0.000	0.000	-0.021	0.000	0.000
Slide	0.000	3.853	2.392	0.000	-0.019	-0.255
Rise	0.000	2.392	13.910	0.000	-0.171	-0.278
Tilt	-0.021	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.019	-0.171	0.000	0.028	0.001
Twist	0.000	-0.255	-0.278	0.000	0.001	0.061

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.029	0.000	0.000	-0.045	0.000	0.000
Slide	0.000	6.449	1.277	0.000	-0.021	-0.232
Rise	0.000	1.277	8.792	0.000	-0.147	-0.239
Tilt	-0.045	0.000	0.000	0.042	0.000	0.000
Roll	0.000	-0.021	-0.147	0.000	0.025	0.002
Twist	0.000	-0.232	-0.239	0.000	0.002	0.070

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.937	0.000	0.000	-0.054	0.000	0.000
Slide	0.000	6.383	1.427	0.000	-0.025	-0.250
Rise	0.000	1.427	8.221	0.000	-0.125	-0.200
Tilt	-0.054	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.025	-0.125	0.000	0.025	-0.001
Twist	0.000	-0.250	-0.200	0.000	-0.001	0.067

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.934	0.000	0.000	-0.064	0.000	0.000
Slide	0.000	5.053	1.600	0.000	-0.034	-0.287
Rise	0.000	1.600	4.117	0.000	-0.028	-0.073
Tilt	-0.064	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.034	-0.028	0.000	0.015	-0.007
Twist	0.000	-0.287	-0.073	0.000	-0.007	0.065

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.088	0.000	0.000	-0.076	0.000	0.000
Slide	0.000	5.282	1.563	0.000	-0.040	-0.298
Rise	0.000	1.563	4.364	0.000	-0.039	-0.067
Tilt	-0.076	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.040	-0.039	0.000	0.015	-0.007
Twist	0.000	-0.298	-0.067	0.000	-0.007	0.064

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.894	0.000	0.000	-0.073	0.000	0.000
Slide	0.000	5.308	1.474	0.000	-0.031	-0.315
Rise	0.000	1.474	4.555	0.000	-0.013	-0.057
Tilt	-0.073	0.000	0.000	0.041	0.000	0.000
Roll	0.000	-0.031	-0.013	0.000	0.016	-0.008
Twist	0.000	-0.315	-0.057	0.000	-0.008	0.067

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.331	0.000	0.000	0.042	0.000	0.000
Slide	0.000	5.580	1.509	0.000	0.027	-0.278
Rise	0.000	1.509	8.467	0.000	-0.198	-0.221
Tilt	0.042	0.000	0.000	0.033	0.000	0.000
Roll	0.000	0.027	-0.198	0.000	0.023	0.004
Twist	0.000	-0.278	-0.221	0.000	0.004	0.072

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.848	0.000	0.000	-0.051	0.000	0.000
Slide	0.000	6.410	1.451	0.000	-0.030	-0.261
Rise	0.000	1.451	8.148	0.000	-0.103	-0.210
Tilt	-0.051	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.030	-0.103	0.000	0.023	0.000
Twist	0.000	-0.261	-0.210	0.000	0.000	0.067

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.783	0.000	0.000	-0.041	0.000	0.000
Slide	0.000	6.377	1.515	0.000	-0.019	-0.251
Rise	0.000	1.515	8.591	0.000	-0.143	-0.233
Tilt	-0.041	0.000	0.000	0.042	0.000	0.000
Roll	0.000	-0.019	-0.143	0.000	0.024	0.001
Twist	0.000	-0.251	-0.233	0.000	0.001	0.069

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.159	0.000	0.000	-0.024	0.000	0.000
Slide	0.000	3.824	2.481	0.000	-0.029	-0.260
Rise	0.000	2.481	13.830	0.000	-0.164	-0.287
Tilt	-0.024	0.000	0.000	0.037	0.000	0.000
Roll	0.000	-0.029	-0.164	0.000	0.025	0.003
Twist	0.000	-0.260	-0.287	0.000	0.003	0.064

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.009	0.000	0.000	-0.013	0.000	0.000
Slide	0.000	3.474	2.557	0.000	-0.049	-0.254
Rise	0.000	2.557	13.269	0.000	-0.198	-0.287
Tilt	-0.013	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.049	-0.198	0.000	0.025	0.004
Twist	0.000	-0.254	-0.287	0.000	0.004	0.057

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.390	0.000	0.000	0.040	0.000	0.000
Slide	0.000	5.329	1.193	0.000	0.033	-0.252
Rise	0.000	1.193	8.281	0.000	-0.193	-0.213
Tilt	0.040	0.000	0.000	0.032	0.000	0.000
Roll	0.000	0.033	-0.193	0.000	0.022	0.003
Twist	0.000	-0.252	-0.213	0.000	0.003	0.071

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.351	0.000	0.000	0.040	0.000	0.000
Slide	0.000	5.150	1.276	0.000	0.015	-0.267
Rise	0.000	1.276	7.937	0.000	-0.162	-0.200
Tilt	0.040	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.015	-0.162	0.000	0.020	0.004
Twist	0.000	-0.267	-0.200	0.000	0.004	0.069

[Image courtesy of Curves+]

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	Mean	Stdev
-aauu.6.cte	0.000	0.000

	Mean	Stdev
-acgu.2.cte	0.000	0.000

	Mean	Stdev
-acgu.7.cte	0.000	0.000

	Mean	Stdev
-ccgg.14.cte	0.000	0.000

	Mean	Stdev
-ccgg.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.15.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-gauc.5.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.4.cte	0.000	0.000

	Mean	Stdev
-guac.11.cte	0.000	0.000

	Mean	Stdev
-guac.16.cte	0.000	0.000

	Mean	Stdev
-ucga.13.cte	0.000	0.000

	Mean	Stdev
-uuaa.12.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.394	0.290

	Mean	Stdev
roll-10-GGCC	7.767	5.175

	Mean	Stdev
shift-10-GGCC	0.000	0.572

	Mean	Stdev
slide-10-GGCC	-1.922	0.332

	Mean	Stdev
tilt-10-GGCC	0.000	3.673

	Mean	Stdev
twist-10-GGCC	29.641	3.311

	Mean	Stdev
rise-11-GUAC	3.296	0.230

	Mean	Stdev
roll-11-GUAC	6.301	5.006

	Mean	Stdev
shift-11-GUAC	0.000	0.716

	Mean	Stdev
slide-11-GUAC	-1.982	0.472

	Mean	Stdev
tilt-11-GUAC	0.000	3.984

	Mean	Stdev
twist-11-GUAC	27.934	3.596

	Mean	Stdev
rise-12-UUAA	3.200	0.310

	Mean	Stdev
roll-12-UUAA	6.910	6.010

	Mean	Stdev
shift-12-UUAA	0.000	0.673

	Mean	Stdev
slide-12-UUAA	-1.684	0.384

	Mean	Stdev
tilt-12-UUAA	0.000	4.715

	Mean	Stdev
twist-12-UUAA	28.063	3.331

	Mean	Stdev
rise-13-UCGA	3.136	0.313

	Mean	Stdev
roll-13-UCGA	5.926	5.848

	Mean	Stdev
shift-13-UCGA	0.000	0.661

	Mean	Stdev
slide-13-UCGA	-1.655	0.372

	Mean	Stdev
tilt-13-UCGA	0.000	4.348

	Mean	Stdev
twist-13-UCGA	28.960	3.246

	Mean	Stdev
rise-14-CCGG	3.390	0.286

	Mean	Stdev
roll-14-CCGG	8.659	5.125

	Mean	Stdev
shift-14-CCGG	0.000	0.544

	Mean	Stdev
slide-14-CCGG	-1.839	0.322

	Mean	Stdev
tilt-14-CCGG	0.000	3.762

	Mean	Stdev
twist-14-CCGG	29.796	3.216

	Mean	Stdev
rise-15-CGCG	3.677	0.404

	Mean	Stdev
roll-15-CGCG	9.112	6.796

	Mean	Stdev
shift-15-CGCG	0.000	0.849

	Mean	Stdev
slide-15-CGCG	-1.719	0.430

	Mean	Stdev
tilt-15-CGCG	0.000	4.471

	Mean	Stdev
twist-15-CGCG	31.574	3.703

	Mean	Stdev
rise-16-GUAC	3.272	0.241

	Mean	Stdev
roll-16-GUAC	6.494	5.172

	Mean	Stdev
shift-16-GUAC	0.000	0.764

	Mean	Stdev
slide-16-GUAC	-1.944	0.516

	Mean	Stdev
tilt-16-GUAC	0.000	4.045

	Mean	Stdev
twist-16-GUAC	27.564	3.929

	Mean	Stdev
rise-2-ACGU	3.289	0.243

	Mean	Stdev
roll-2-ACGU	7.610	5.141

	Mean	Stdev
shift-2-ACGU	0.000	0.705

	Mean	Stdev
slide-2-ACGU	-1.659	0.495

	Mean	Stdev
tilt-2-ACGU	0.000	3.979

	Mean	Stdev
twist-2-ACGU	28.254	3.580

	Mean	Stdev
rise-3-CGCG	3.637	0.390

	Mean	Stdev
roll-3-CGCG	10.762	6.672

	Mean	Stdev
shift-3-CGCG	0.000	0.794

	Mean	Stdev
slide-3-CGCG	-1.620	0.423

	Mean	Stdev
tilt-3-CGCG	0.000	4.332

	Mean	Stdev
twist-3-CGCG	32.078	3.773

	Mean	Stdev
rise-4-GGCC	3.410	0.290

	Mean	Stdev
roll-4-GGCC	9.104	5.182

	Mean	Stdev
shift-4-GGCC	0.000	0.580

	Mean	Stdev
slide-4-GGCC	-1.835	0.329

	Mean	Stdev
tilt-4-GGCC	0.000	3.775

	Mean	Stdev
twist-4-GGCC	30.163	3.248

	Mean	Stdev
rise-5-GAUC	3.134	0.311

	Mean	Stdev
roll-5-GAUC	5.518	5.752

	Mean	Stdev
shift-5-GAUC	0.000	0.678

	Mean	Stdev
slide-5-GAUC	-1.649	0.370

	Mean	Stdev
tilt-5-GAUC	0.000	4.304

	Mean	Stdev
twist-5-GAUC	29.178	3.260

	Mean	Stdev
rise-6-AAUU	3.204	0.313

	Mean	Stdev
roll-6-AAUU	6.335	5.896

	Mean	Stdev
shift-6-AAUU	0.000	0.677

	Mean	Stdev
slide-6-AAUU	-1.692	0.385

	Mean	Stdev
tilt-6-AAUU	0.000	4.822

	Mean	Stdev
twist-6-AAUU	28.029	3.380

	Mean	Stdev
rise-7-ACGU	3.292	0.229

	Mean	Stdev
roll-7-ACGU	5.830	4.790

	Mean	Stdev
shift-7-ACGU	0.000	0.712

	Mean	Stdev
slide-7-ACGU	-2.001	0.469

	Mean	Stdev
tilt-7-ACGU	0.000	4.040

	Mean	Stdev
twist-7-ACGU	27.780	3.700

	Mean	Stdev
rise-8-CCGG	3.407	0.291

	Mean	Stdev
roll-8-CCGG	7.493	5.041

	Mean	Stdev
shift-8-CCGG	0.000	0.560

	Mean	Stdev
slide-8-CCGG	-1.922	0.330

	Mean	Stdev
tilt-8-CCGG	0.000	3.701

	Mean	Stdev
twist-8-CCGG	29.726	3.291

	Mean	Stdev
rise-9-CGCG	3.599	0.377

	Mean	Stdev
roll-9-CGCG	5.972	6.406

	Mean	Stdev
shift-9-CGCG	0.000	0.876

	Mean	Stdev
slide-9-CGCG	-1.749	0.424

	Mean	Stdev
tilt-9-CGCG	0.000	4.117

	Mean	Stdev
twist-9-CGCG	30.339	3.701

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