NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.929	0.000	0.000	-0.062	0.000	0.000
Slide	0.000	4.590	3.695	0.000	-0.011	-0.074
Rise	0.000	3.695	11.621	0.000	0.079	-0.247
Tilt	-0.062	0.000	0.000	0.042	0.000	0.000
Roll	0.000	-0.011	0.079	0.000	0.031	0.012
Twist	0.000	-0.074	-0.247	0.000	0.012	0.041

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.084	0.000	0.000	-0.114	0.000	0.000
Slide	0.000	2.745	0.525	0.000	0.011	-0.146
Rise	0.000	0.526	8.961	0.000	-0.113	-0.277
Tilt	-0.114	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.011	-0.113	0.000	0.020	0.008
Twist	0.000	-0.146	-0.277	0.000	0.008	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.490	0.000	0.000	-0.159	0.000	0.000
Slide	0.000	3.090	1.614	0.000	0.030	-0.120
Rise	0.000	1.614	6.912	0.000	-0.069	-0.186
Tilt	-0.159	0.000	0.000	0.034	0.000	0.000
Roll	0.000	0.030	-0.069	0.000	0.015	0.002
Twist	0.000	-0.120	-0.186	0.000	0.002	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.056	0.000	0.000	-0.120	0.000	0.000
Slide	0.000	2.929	0.303	0.000	0.010	-0.147
Rise	0.000	0.303	9.278	0.000	-0.122	-0.285
Tilt	-0.120	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.010	-0.122	0.000	0.020	0.009
Twist	0.000	-0.147	-0.285	0.000	0.009	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.883	0.000	0.000	-0.121	0.000	0.000
Slide	0.000	3.021	1.317	0.000	-0.059	-0.123
Rise	0.000	1.317	9.301	0.000	-0.046	-0.169
Tilt	-0.121	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.059	-0.046	0.000	0.022	0.011
Twist	0.000	-0.123	-0.169	0.000	0.011	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.985	0.000	0.000	-0.111	0.000	0.000
Slide	0.000	3.148	2.193	0.000	0.072	-0.101
Rise	0.000	2.193	11.466	0.000	0.152	-0.234
Tilt	-0.111	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.072	0.152	0.000	0.024	0.002
Twist	0.000	-0.101	-0.234	0.000	0.002	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.910	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	3.142	2.023	0.000	0.081	-0.110
Rise	0.000	2.024	11.133	0.000	0.144	-0.225
Tilt	-0.106	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.081	0.144	0.000	0.023	0.001
Twist	0.000	-0.110	-0.225	0.000	0.001	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.938	0.000	0.000	-0.056	0.000	0.000
Slide	0.000	4.366	3.742	0.000	-0.012	-0.082
Rise	0.000	3.742	11.710	0.000	0.071	-0.234
Tilt	-0.056	0.000	0.000	0.043	0.000	0.000
Roll	0.000	-0.012	0.071	0.000	0.030	0.011
Twist	0.000	-0.082	-0.234	0.000	0.011	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.858	0.000	0.000	-0.116	0.000	0.000
Slide	0.000	2.914	1.270	0.000	-0.066	-0.106
Rise	0.000	1.270	8.971	0.000	-0.056	-0.164
Tilt	-0.116	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.066	-0.056	0.000	0.025	0.012
Twist	0.000	-0.106	-0.164	0.000	0.012	0.038

[Image courtesy of Curves+]

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	Mean	Stdev
-acgt.5.cte	0.000	0.000

	Mean	Stdev
-cgcg.3.cte	0.000	0.000

	Mean	Stdev
-cgcg.6.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-gatc.4.cte	0.000	0.000

	Mean	Stdev
-gcgc.10.cte	0.000	0.000

	Mean	Stdev
-gcgc.2.cte	0.000	0.000

	Mean	Stdev
-gtac.7.cte	0.000	0.000

	Mean	Stdev
-tcga.8.cte	0.000	0.000

	Mean	Stdev
rise-10-GCGC	3.442	0.266

	Mean	Stdev
roll-10-GCGC	-3.060	5.482

	Mean	Stdev
shift-10-GCGC	0.000	0.918

	Mean	Stdev
slide-10-GCGC	-0.286	0.487

	Mean	Stdev
tilt-10-GCGC	0.000	4.429

	Mean	Stdev
twist-10-GCGC	34.578	4.903

	Mean	Stdev
rise-2-GCGC	3.445	0.264

	Mean	Stdev
roll-2-GCGC	-3.399	5.585

	Mean	Stdev
shift-2-GCGC	0.000	0.957

	Mean	Stdev
slide-2-GCGC	-0.233	0.493

	Mean	Stdev
tilt-2-GCGC	0.000	4.494

	Mean	Stdev
twist-2-GCGC	34.865	4.714

	Mean	Stdev
rise-3-CGCG	3.033	0.322

	Mean	Stdev
roll-3-CGCG	6.866	6.009

	Mean	Stdev
shift-3-CGCG	0.000	0.977

	Mean	Stdev
slide-3-CGCG	0.106	0.590

	Mean	Stdev
tilt-3-CGCG	0.000	6.111

	Mean	Stdev
twist-3-CGCG	30.258	7.309

	Mean	Stdev
rise-4-GATC	3.317	0.265

	Mean	Stdev
roll-4-GATC	2.007	5.495

	Mean	Stdev
shift-4-GATC	0.000	1.109

	Mean	Stdev
slide-4-GATC	0.387	0.481

	Mean	Stdev
tilt-4-GATC	0.000	5.505

	Mean	Stdev
twist-4-GATC	38.180	4.370

	Mean	Stdev
rise-5-ACGT	3.323	0.288

	Mean	Stdev
roll-5-ACGT	-0.428	4.801

	Mean	Stdev
shift-5-ACGT	0.000	0.836

	Mean	Stdev
slide-5-ACGT	-0.691	0.418

	Mean	Stdev
tilt-5-ACGT	0.000	3.936

	Mean	Stdev
twist-5-ACGT	30.988	4.413

	Mean	Stdev
rise-6-CGCG	3.638	0.340

	Mean	Stdev
roll-6-CGCG	7.503	6.579

	Mean	Stdev
shift-6-CGCG	0.000	0.886

	Mean	Stdev
slide-6-CGCG	-0.120	0.503

	Mean	Stdev
tilt-6-CGCG	0.000	5.873

	Mean	Stdev
twist-6-CGCG	41.932	5.308

	Mean	Stdev
rise-7-GTAC	3.333	0.284

	Mean	Stdev
roll-7-GTAC	-0.551	4.824

	Mean	Stdev
shift-7-GTAC	0.000	0.823

	Mean	Stdev
slide-7-GTAC	-0.717	0.433

	Mean	Stdev
tilt-7-GTAC	0.000	3.856

	Mean	Stdev
twist-7-GTAC	31.295	4.306

	Mean	Stdev
rise-8-TCGA	3.314	0.270

	Mean	Stdev
roll-8-TCGA	2.079	5.229

	Mean	Stdev
shift-8-TCGA	0.000	1.107

	Mean	Stdev
slide-8-TCGA	0.377	0.485

	Mean	Stdev
tilt-8-TCGA	0.000	5.457

	Mean	Stdev
twist-8-TCGA	38.076	4.471

	Mean	Stdev
rise-9-CGCG	3.037	0.326

	Mean	Stdev
roll-9-CGCG	6.489	6.227

	Mean	Stdev
shift-9-CGCG	0.000	1.040

	Mean	Stdev
slide-9-CGCG	0.191	0.586

	Mean	Stdev
tilt-9-CGCG	0.000	6.407

	Mean	Stdev
twist-9-CGCG	30.936	7.738

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