NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.107	0.000	0.000	-0.027	0.000	0.000
Slide	0.000	2.166	1.737	0.000	-0.069	-0.095
Rise	0.000	1.737	6.806	0.000	-0.036	-0.191
Tilt	-0.027	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.069	-0.036	0.000	0.020	0.004
Twist	0.000	-0.095	-0.191	0.000	0.004	0.024

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.328	0.000	0.000	-0.082	0.000	0.000
Slide	0.000	2.469	1.577	0.000	-0.071	-0.135
Rise	0.000	1.577	6.495	0.000	-0.024	-0.162
Tilt	-0.082	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.071	-0.024	0.000	0.021	0.005
Twist	0.000	-0.135	-0.162	0.000	0.005	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.107	0.000	0.000	-0.006	0.000	0.000
Slide	0.000	2.221	1.645	0.000	-0.076	-0.102
Rise	0.000	1.645	7.116	0.000	-0.042	-0.169
Tilt	-0.006	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.076	-0.042	0.000	0.022	0.008
Twist	0.000	-0.102	-0.169	0.000	0.008	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.018	0.000	0.000	-0.036	0.000	0.000
Slide	0.000	2.061	1.645	0.000	-0.072	-0.108
Rise	0.000	1.646	7.152	0.000	-0.038	-0.203
Tilt	-0.036	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.072	-0.038	0.000	0.020	0.007
Twist	0.000	-0.108	-0.203	0.000	0.007	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.778	0.000	0.000	-0.088	0.000	0.000
Slide	0.000	2.376	0.191	0.000	0.001	-0.085
Rise	0.000	0.191	3.109	0.000	0.022	-0.004
Tilt	-0.088	0.000	0.000	0.024	0.000	0.000
Roll	0.000	0.001	0.022	0.000	0.011	-0.002
Twist	0.000	-0.085	-0.004	0.000	-0.002	0.007

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.926	0.000	0.000	-0.084	0.000	0.000
Slide	0.000	1.994	1.011	0.000	-0.047	-0.123
Rise	0.000	1.011	7.858	0.000	-0.028	-0.143
Tilt	-0.084	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.047	-0.028	0.000	0.020	0.011
Twist	0.000	-0.123	-0.143	0.000	0.011	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.018	0.000	0.000	-0.091	0.000	0.000
Slide	0.000	1.947	1.319	0.000	-0.030	-0.114
Rise	0.000	1.319	7.749	0.000	-0.053	-0.181
Tilt	-0.091	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.030	-0.053	0.000	0.020	0.011
Twist	0.000	-0.114	-0.181	0.000	0.011	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.785	0.000	0.000	-0.104	0.000	0.000
Slide	0.000	2.367	1.021	0.000	-0.044	-0.121
Rise	0.000	1.021	7.397	0.000	-0.040	-0.133
Tilt	-0.104	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.044	-0.040	0.000	0.022	0.011
Twist	0.000	-0.121	-0.133	0.000	0.011	0.036

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.834	0.000	0.000	-0.070	0.000	0.000
Slide	0.000	1.824	0.987	0.000	-0.052	-0.126
Rise	0.000	0.987	7.832	0.000	-0.037	-0.164
Tilt	-0.070	0.000	0.000	0.029	0.000	0.000
Roll	0.000	-0.052	-0.037	0.000	0.020	0.011
Twist	0.000	-0.126	-0.164	0.000	0.011	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.062	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	1.555	1.815	0.000	-0.004	-0.082
Rise	0.000	1.815	6.978	0.000	-0.028	-0.198
Tilt	-0.095	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.004	-0.028	0.000	0.019	0.007
Twist	0.000	-0.082	-0.198	0.000	0.007	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.971	0.000	0.000	-0.089	0.000	0.000
Slide	0.000	1.539	1.653	0.000	-0.003	-0.101
Rise	0.000	1.653	7.571	0.000	-0.007	-0.203
Tilt	-0.089	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.003	-0.007	0.000	0.016	0.002
Twist	0.000	-0.101	-0.203	0.000	0.002	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.986	0.000	0.000	-0.095	0.000	0.000
Slide	0.000	1.514	1.650	0.000	-0.010	-0.084
Rise	0.000	1.650	7.020	0.000	-0.029	-0.188
Tilt	-0.095	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.010	-0.029	0.000	0.017	0.006
Twist	0.000	-0.084	-0.188	0.000	0.006	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.572	0.000	0.000	-0.057	0.000	0.000
Slide	0.000	1.689	1.094	0.000	-0.003	-0.113
Rise	0.000	1.094	6.093	0.000	0.029	-0.118
Tilt	-0.057	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.003	0.029	0.000	0.013	-0.001
Twist	0.000	-0.113	-0.118	0.000	-0.001	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.900	0.000	0.000	-0.035	0.000	0.000
Slide	0.000	1.439	1.652	0.000	-0.005	-0.060
Rise	0.000	1.652	6.593	0.000	0.004	-0.170
Tilt	-0.035	0.000	0.000	0.026	0.000	0.000
Roll	0.000	-0.005	0.004	0.000	0.016	0.004
Twist	0.000	-0.060	-0.170	0.000	0.004	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.108	0.000	0.000	-0.099	0.000	0.000
Slide	0.000	1.653	1.690	0.000	-0.014	-0.124
Rise	0.000	1.690	7.584	0.000	-0.000	-0.180
Tilt	-0.099	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.014	-0.000	0.000	0.019	0.005
Twist	0.000	-0.124	-0.180	0.000	0.005	0.030

[Image courtesy of Curves+]

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	Mean	Stdev
-agct.12.cte	0.000	0.000

	Mean	Stdev
-agct.16.cte	0.000	0.000

	Mean	Stdev
-agct.4.cte	0.000	0.000

	Mean	Stdev
-agct.8.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-gatc.11.cte	0.000	0.000

	Mean	Stdev
-gatc.15.cte	0.000	0.000

	Mean	Stdev
-gatc.3.cte	0.000	0.000

	Mean	Stdev
-gatc.7.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.13.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.5.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
-ggcc.9.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.451	0.367

	Mean	Stdev
roll-10-GGCC	4.862	6.005

	Mean	Stdev
shift-10-GGCC	0.000	0.855

	Mean	Stdev
slide-10-GGCC	-0.712	0.756

	Mean	Stdev
tilt-10-GGCC	0.000	4.718

	Mean	Stdev
twist-10-GGCC	31.788	5.948

	Mean	Stdev
rise-11-GATC	3.298	0.289

	Mean	Stdev
roll-11-GATC	0.529	5.957

	Mean	Stdev
shift-11-GATC	0.000	0.918

	Mean	Stdev
slide-11-GATC	-0.191	0.632

	Mean	Stdev
tilt-11-GATC	0.000	5.046

	Mean	Stdev
twist-11-GATC	36.527	5.618

	Mean	Stdev
rise-12-AGCT	3.262	0.353

	Mean	Stdev
roll-12-AGCT	2.276	5.787

	Mean	Stdev
shift-12-AGCT	0.000	0.736

	Mean	Stdev
slide-12-AGCT	-0.814	0.633

	Mean	Stdev
tilt-12-AGCT	0.000	4.606

	Mean	Stdev
twist-12-AGCT	30.338	5.865

	Mean	Stdev
rise-13-GGCC	3.417	0.334

	Mean	Stdev
roll-13-GGCC	2.588	6.066

	Mean	Stdev
shift-13-GGCC	0.000	0.897

	Mean	Stdev
slide-13-GGCC	-0.624	0.767

	Mean	Stdev
tilt-13-GGCC	0.000	4.896

	Mean	Stdev
twist-13-GGCC	33.924	6.258

	Mean	Stdev
rise-14-GGCC	3.419	0.351

	Mean	Stdev
roll-14-GGCC	5.001	6.164

	Mean	Stdev
shift-14-GGCC	0.000	0.892

	Mean	Stdev
slide-14-GGCC	-0.535	0.749

	Mean	Stdev
tilt-14-GGCC	0.000	4.662

	Mean	Stdev
twist-14-GGCC	32.256	5.865

	Mean	Stdev
rise-15-GATC	3.335	0.304

	Mean	Stdev
roll-15-GATC	1.505	6.015

	Mean	Stdev
shift-15-GATC	0.000	0.883

	Mean	Stdev
slide-15-GATC	-0.235	0.641

	Mean	Stdev
tilt-15-GATC	0.000	5.029

	Mean	Stdev
twist-15-GATC	35.798	5.992

	Mean	Stdev
rise-16-AGCT	3.399	0.337

	Mean	Stdev
roll-16-AGCT	1.978	5.584

	Mean	Stdev
shift-16-AGCT	0.000	0.727

	Mean	Stdev
slide-16-AGCT	-0.813	0.608

	Mean	Stdev
tilt-16-AGCT	0.000	4.769

	Mean	Stdev
twist-16-AGCT	32.594	5.566

	Mean	Stdev
rise-2-CGCG	3.122	0.439

	Mean	Stdev
roll-2-CGCG	7.392	7.488

	Mean	Stdev
shift-2-CGCG	0.000	1.131

	Mean	Stdev
slide-2-CGCG	0.288	0.674

	Mean	Stdev
tilt-2-CGCG	0.000	6.400

	Mean	Stdev
twist-2-CGCG	36.442	12.749

	Mean	Stdev
rise-3-GATC	3.358	0.295

	Mean	Stdev
roll-3-GATC	2.345	5.665

	Mean	Stdev
shift-3-GATC	0.000	1.128

	Mean	Stdev
slide-3-GATC	0.171	0.554

	Mean	Stdev
tilt-3-GATC	0.000	5.471

	Mean	Stdev
twist-3-GATC	36.531	4.794

	Mean	Stdev
rise-4-AGCT	3.307	0.324

	Mean	Stdev
roll-4-AGCT	3.067	5.630

	Mean	Stdev
shift-4-AGCT	0.000	0.728

	Mean	Stdev
slide-4-AGCT	-0.702	0.610

	Mean	Stdev
tilt-4-AGCT	0.000	4.030

	Mean	Stdev
twist-4-AGCT	30.762	4.558

	Mean	Stdev
rise-5-GGCC	3.539	0.336

	Mean	Stdev
roll-5-GGCC	1.315	6.897

	Mean	Stdev
shift-5-GGCC	0.000	1.124

	Mean	Stdev
slide-5-GGCC	-0.937	0.748

	Mean	Stdev
tilt-5-GGCC	0.000	4.880

	Mean	Stdev
twist-5-GGCC	32.111	6.553

	Mean	Stdev
rise-6-GGCC	3.464	0.378

	Mean	Stdev
roll-6-GGCC	4.032	6.238

	Mean	Stdev
shift-6-GGCC	0.000	0.829

	Mean	Stdev
slide-6-GGCC	-0.850	0.762

	Mean	Stdev
tilt-6-GGCC	0.000	4.898

	Mean	Stdev
twist-6-GGCC	33.033	5.992

	Mean	Stdev
rise-7-GATC	3.269	0.292

	Mean	Stdev
roll-7-GATC	1.568	6.109

	Mean	Stdev
shift-7-GATC	0.000	0.941

	Mean	Stdev
slide-7-GATC	-0.198	0.674

	Mean	Stdev
tilt-7-GATC	0.000	5.074

	Mean	Stdev
twist-7-GATC	35.539	5.684

	Mean	Stdev
rise-8-AGCT	3.237	0.341

	Mean	Stdev
roll-8-AGCT	2.733	5.889

	Mean	Stdev
shift-8-AGCT	0.000	0.776

	Mean	Stdev
slide-8-AGCT	-0.725	0.652

	Mean	Stdev
tilt-8-AGCT	0.000	4.558

	Mean	Stdev
twist-8-AGCT	29.788	6.004

	Mean	Stdev
rise-9-GGCC	3.480	0.321

	Mean	Stdev
roll-9-GGCC	3.481	5.721

	Mean	Stdev
shift-9-GGCC	0.000	0.837

	Mean	Stdev
slide-9-GGCC	-0.689	0.766

	Mean	Stdev
tilt-9-GGCC	0.000	4.623

	Mean	Stdev
twist-9-GGCC	35.063	5.519

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