NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.862	0.000	0.000	-0.035	0.000	0.000
Slide	0.000	3.936	3.190	0.000	0.026	-0.069
Rise	0.000	3.190	11.458	0.000	0.095	-0.214
Tilt	-0.035	0.000	0.000	0.042	0.000	0.000
Roll	0.000	0.026	0.095	0.000	0.027	0.011
Twist	0.000	-0.069	-0.214	0.000	0.011	0.034

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.963	0.000	0.000	-0.017	0.000	0.000
Slide	0.000	5.131	3.340	0.000	-0.076	-0.085
Rise	0.000	3.340	11.511	0.000	0.042	-0.207
Tilt	-0.017	0.000	0.000	0.045	0.000	0.000
Roll	0.000	-0.076	0.042	0.000	0.031	0.013
Twist	0.000	-0.085	-0.207	0.000	0.013	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.769	0.000	0.000	-0.092	0.000	0.000
Slide	0.000	2.255	1.113	0.000	0.029	-0.101
Rise	0.000	1.113	6.688	0.000	-0.082	-0.189
Tilt	-0.092	0.000	0.000	0.028	0.000	0.000
Roll	0.000	0.029	-0.082	0.000	0.014	0.005
Twist	0.000	-0.101	-0.189	0.000	0.005	0.024

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.723	0.000	0.000	-0.091	0.000	0.000
Slide	0.000	2.270	1.319	0.000	0.025	-0.090
Rise	0.000	1.319	7.140	0.000	-0.065	-0.178
Tilt	-0.091	0.000	0.000	0.031	0.000	0.000
Roll	0.000	0.025	-0.065	0.000	0.016	0.006
Twist	0.000	-0.090	-0.178	0.000	0.006	0.026

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.213	0.000	0.000	-0.074	0.000	0.000
Slide	0.000	1.746	1.845	0.000	0.009	-0.086
Rise	0.000	1.845	7.790	0.000	-0.062	-0.228
Tilt	-0.074	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.009	-0.062	0.000	0.021	0.004
Twist	0.000	-0.086	-0.228	0.000	0.004	0.028

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.191	0.000	0.000	-0.122	0.000	0.000
Slide	0.000	2.462	1.283	0.000	-0.040	-0.085
Rise	0.000	1.283	6.361	0.000	-0.104	-0.207
Tilt	-0.122	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.040	-0.104	0.000	0.014	0.004
Twist	0.000	-0.085	-0.207	0.000	0.004	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.824	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	2.665	1.840	0.000	-0.062	-0.163
Rise	0.000	1.840	9.078	0.000	0.005	-0.156
Tilt	-0.059	0.000	0.000	0.027	0.000	0.000
Roll	0.000	-0.062	0.005	0.000	0.022	0.004
Twist	0.000	-0.163	-0.156	0.000	0.004	0.025

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.311	0.000	0.000	-0.140	0.000	0.000
Slide	0.000	2.860	2.976	0.000	0.057	-0.089
Rise	0.000	2.976	11.683	0.000	0.123	-0.249
Tilt	-0.140	0.000	0.000	0.046	0.000	0.000
Roll	0.000	0.057	0.123	0.000	0.026	0.005
Twist	0.000	-0.089	-0.249	0.000	0.005	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.214	0.000	0.000	-0.106	0.000	0.000
Slide	0.000	1.805	1.365	0.000	-0.044	-0.110
Rise	0.000	1.365	8.772	0.000	-0.055	-0.148
Tilt	-0.106	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.044	-0.055	0.000	0.022	0.011
Twist	0.000	-0.110	-0.148	0.000	0.011	0.038

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.765	0.000	0.000	-0.070	0.000	0.000
Slide	0.000	3.048	0.514	0.000	0.017	-0.132
Rise	0.000	0.514	8.969	0.000	-0.138	-0.247
Tilt	-0.070	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.017	-0.138	0.000	0.020	0.011
Twist	0.000	-0.132	-0.247	0.000	0.011	0.026

[Image courtesy of Curves+]

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	Mean	Stdev
-acgt.3.cte	0.000	0.000

	Mean	Stdev
-atat.5.cte	0.000	0.000

	Mean	Stdev
-catg.2.cte	0.000	0.000

	Mean	Stdev
-catg.4.cte	0.000	0.000

	Mean	Stdev
-ccgg.7.cte	0.000	0.000

	Mean	Stdev
-ctag.11.cte	0.000	0.000

	Mean	Stdev
-ctag.8.cte	0.000	0.000

	Mean	Stdev
-gcgc.10.cte	0.000	0.000

	Mean	Stdev
-tcga.6.cte	0.000	0.000

	Mean	Stdev
-tgca.9.cte	0.000	0.000

	Mean	Stdev
rise-10-GCGC	3.464	0.278

	Mean	Stdev
roll-10-GCGC	-1.727	5.045

	Mean	Stdev
shift-10-GCGC	0.000	0.814

	Mean	Stdev
slide-10-GCGC	-0.410	0.536

	Mean	Stdev
tilt-10-GCGC	0.000	4.359

	Mean	Stdev
twist-10-GCGC	36.284	4.214

	Mean	Stdev
rise-11-CTAG	3.435	0.379

	Mean	Stdev
roll-11-CTAG	5.073	6.976

	Mean	Stdev
shift-11-CTAG	0.000	0.951

	Mean	Stdev
slide-11-CTAG	-0.414	0.533

	Mean	Stdev
tilt-11-CTAG	0.000	6.246

	Mean	Stdev
twist-11-CTAG	35.097	6.101

	Mean	Stdev
rise-2-CATG	3.383	0.345

	Mean	Stdev
roll-2-CATG	6.194	7.294

	Mean	Stdev
shift-2-CATG	0.000	1.122

	Mean	Stdev
slide-2-CATG	-0.065	0.604

	Mean	Stdev
tilt-2-CATG	0.000	5.864

	Mean	Stdev
twist-2-CATG	37.510	6.151

	Mean	Stdev
rise-3-ACGT	3.211	0.282

	Mean	Stdev
roll-3-ACGT	-0.059	5.282

	Mean	Stdev
shift-3-ACGT	0.000	0.839

	Mean	Stdev
slide-3-ACGT	-0.474	0.439

	Mean	Stdev
tilt-3-ACGT	0.000	3.797

	Mean	Stdev
twist-3-ACGT	29.792	4.959

	Mean	Stdev
rise-4-CATG	3.423	0.325

	Mean	Stdev
roll-4-CATG	5.244	6.573

	Mean	Stdev
shift-4-CATG	0.000	1.134

	Mean	Stdev
slide-4-CATG	-0.033	0.583

	Mean	Stdev
tilt-4-CATG	0.000	5.478

	Mean	Stdev
twist-4-CATG	39.061	5.650

	Mean	Stdev
rise-5-ATAT	3.237	0.269

	Mean	Stdev
roll-5-ATAT	-1.726	4.892

	Mean	Stdev
shift-5-ATAT	0.000	0.547

	Mean	Stdev
slide-5-ATAT	-0.816	0.387

	Mean	Stdev
tilt-5-ATAT	0.000	3.635

	Mean	Stdev
twist-5-ATAT	30.020	4.274

	Mean	Stdev
rise-6-TCGA	3.383	0.278

	Mean	Stdev
roll-6-TCGA	0.973	5.567

	Mean	Stdev
shift-6-TCGA	0.000	0.813

	Mean	Stdev
slide-6-TCGA	-0.190	0.654

	Mean	Stdev
tilt-6-TCGA	0.000	4.849

	Mean	Stdev
twist-6-TCGA	38.467	4.658

	Mean	Stdev
rise-7-CCGG	3.551	0.344

	Mean	Stdev
roll-7-CCGG	4.429	5.454

	Mean	Stdev
shift-7-CCGG	0.000	0.763

	Mean	Stdev
slide-7-CCGG	-0.809	0.692

	Mean	Stdev
tilt-7-CCGG	0.000	5.159

	Mean	Stdev
twist-7-CCGG	33.607	5.434

	Mean	Stdev
rise-8-CTAG	3.236	0.278

	Mean	Stdev
roll-8-CTAG	1.376	5.391

	Mean	Stdev
shift-8-CTAG	0.000	0.920

	Mean	Stdev
slide-8-CTAG	-0.382	0.633

	Mean	Stdev
tilt-8-CTAG	0.000	5.105

	Mean	Stdev
twist-8-CTAG	32.882	6.281

	Mean	Stdev
rise-9-TGCA	3.068	0.304

	Mean	Stdev
roll-9-TGCA	6.887	6.691

	Mean	Stdev
shift-9-TGCA	0.000	0.995

	Mean	Stdev
slide-9-TGCA	-0.028	0.547

	Mean	Stdev
tilt-9-TGCA	0.000	5.142

	Mean	Stdev
twist-9-TGCA	31.122	7.511

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