NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.755	0.000	0.000	-0.103	0.000	0.000
Slide	0.000	2.892	0.936	0.000	-0.067	-0.136
Rise	0.000	0.936	9.725	0.000	-0.040	-0.177
Tilt	-0.103	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.067	-0.040	0.000	0.022	0.010
Twist	0.000	-0.136	-0.177	0.000	0.010	0.041

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.365	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	3.872	3.215	0.000	0.011	-0.092
Rise	0.000	3.215	11.050	0.000	0.048	-0.240
Tilt	-0.055	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.011	0.048	0.000	0.026	0.010
Twist	0.000	-0.092	-0.240	0.000	0.010	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.085	0.000	0.000	-0.087	0.000	0.000
Slide	0.000	2.539	1.656	0.000	-0.041	-0.092
Rise	0.000	1.656	6.576	0.000	-0.076	-0.166
Tilt	-0.087	0.000	0.000	0.031	0.000	0.000
Roll	0.000	-0.041	-0.076	0.000	0.019	0.003
Twist	0.000	-0.092	-0.166	0.000	0.003	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.338	0.000	0.000	-0.010	0.000	0.000
Slide	0.000	5.011	2.976	0.000	-0.062	-0.107
Rise	0.000	2.976	11.777	0.000	0.069	-0.167
Tilt	-0.010	0.000	0.000	0.046	0.000	0.000
Roll	0.000	-0.062	0.069	0.000	0.031	0.014
Twist	0.000	-0.107	-0.167	0.000	0.014	0.049

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.666	0.000	0.000	-0.021	0.000	0.000
Slide	0.000	5.012	2.904	0.000	-0.014	-0.150
Rise	0.000	2.904	11.814	0.000	0.092	-0.151
Tilt	-0.021	0.000	0.000	0.047	0.000	0.000
Roll	0.000	-0.014	0.092	0.000	0.034	0.009
Twist	0.000	-0.150	-0.151	0.000	0.009	0.053

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.531	0.000	0.000	0.007	0.000	0.000
Slide	0.000	5.164	2.893	0.000	-0.056	-0.102
Rise	0.000	2.893	11.782	0.000	0.078	-0.170
Tilt	0.007	0.000	0.000	0.046	0.000	0.000
Roll	0.000	-0.056	0.078	0.000	0.029	0.014
Twist	0.000	-0.102	-0.170	0.000	0.014	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.093	0.000	0.000	-0.140	0.000	0.000
Slide	0.000	2.593	1.126	0.000	0.024	-0.082
Rise	0.000	1.126	7.616	0.000	-0.089	-0.240
Tilt	-0.140	0.000	0.000	0.031	0.000	0.000
Roll	0.000	0.024	-0.089	0.000	0.016	0.006
Twist	0.000	-0.082	-0.240	0.000	0.006	0.027

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.443	0.000	0.000	-0.077	0.000	0.000
Slide	0.000	2.389	1.399	0.000	-0.004	-0.182
Rise	0.000	1.399	7.630	0.000	-0.042	-0.136
Tilt	-0.077	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.004	-0.042	0.000	0.021	0.006
Twist	0.000	-0.182	-0.136	0.000	0.006	0.044

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.940	0.000	0.000	-0.128	0.000	0.000
Slide	0.000	2.024	1.543	0.000	-0.060	-0.099
Rise	0.000	1.543	8.884	0.000	-0.030	-0.181
Tilt	-0.128	0.000	0.000	0.035	0.000	0.000
Roll	0.000	-0.060	-0.030	0.000	0.021	0.011
Twist	0.000	-0.099	-0.181	0.000	0.011	0.034

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.635	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	1.984	1.037	0.000	0.046	-0.092
Rise	0.000	1.037	7.314	0.000	-0.081	-0.220
Tilt	-0.055	0.000	0.000	0.029	0.000	0.000
Roll	0.000	0.046	-0.081	0.000	0.017	0.009
Twist	0.000	-0.092	-0.220	0.000	0.009	0.031

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.432	0.000	0.000	-0.059	0.000	0.000
Slide	0.000	2.527	1.278	0.000	0.017	-0.140
Rise	0.000	1.278	7.633	0.000	-0.084	-0.231
Tilt	-0.059	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.017	-0.084	0.000	0.017	0.012
Twist	0.000	-0.140	-0.231	0.000	0.012	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.085	0.000	0.000	-0.082	0.000	0.000
Slide	0.000	4.022	1.489	0.000	-0.039	-0.286
Rise	0.000	1.489	7.480	0.000	-0.087	-0.163
Tilt	-0.082	0.000	0.000	0.028	0.000	0.000
Roll	0.000	-0.039	-0.087	0.000	0.024	0.004
Twist	0.000	-0.286	-0.163	0.000	0.004	0.057

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.049	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	3.146	0.713	0.000	-0.039	-0.125
Rise	0.000	0.713	6.291	0.000	-0.071	-0.129
Tilt	-0.094	0.000	0.000	0.030	0.000	0.000
Roll	0.000	-0.039	-0.071	0.000	0.016	0.004
Twist	0.000	-0.125	-0.129	0.000	0.004	0.024

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.6.cte	0.000	0.000

	Mean	Stdev
-acgt.7.cte	0.000	0.000

	Mean	Stdev
-agct.2.cte	0.000	0.000

	Mean	Stdev
-atat.10.cte	0.000	0.000

	Mean	Stdev
-atat.12.cte	0.000	0.000

	Mean	Stdev
-atat.4.cte	0.000	0.000

	Mean	Stdev
-cgcg.8.cte	0.000	0.000

	Mean	Stdev
-gatc.3.cte	0.000	0.000

	Mean	Stdev
-gatc.9.cte	0.000	0.000

	Mean	Stdev
-tata.11.cte	0.000	0.000

	Mean	Stdev
-tata.5.cte	0.000	0.000

	Mean	Stdev
-ttaa.13.cte	0.000	0.000

	Mean	Stdev
-ttaa.14.cte	0.000	0.000

	Mean	Stdev
rise-10-ATAT	3.234	0.254

	Mean	Stdev
roll-10-ATAT	-1.684	4.847

	Mean	Stdev
shift-10-ATAT	0.000	0.663

	Mean	Stdev
slide-10-ATAT	-0.797	0.381

	Mean	Stdev
tilt-10-ATAT	0.000	3.580

	Mean	Stdev
twist-10-ATAT	30.740	3.874

	Mean	Stdev
rise-11-TATA	3.457	0.324

	Mean	Stdev
roll-11-TATA	3.629	7.344

	Mean	Stdev
shift-11-TATA	0.000	1.050

	Mean	Stdev
slide-11-TATA	-0.165	0.673

	Mean	Stdev
tilt-11-TATA	0.000	4.953

	Mean	Stdev
twist-11-TATA	39.899	5.878

	Mean	Stdev
rise-12-ATAT	3.261	0.246

	Mean	Stdev
roll-12-ATAT	-2.047	4.356

	Mean	Stdev
shift-12-ATAT	0.000	0.595

	Mean	Stdev
slide-12-ATAT	-0.831	0.381

	Mean	Stdev
tilt-12-ATAT	0.000	3.549

	Mean	Stdev
twist-12-ATAT	31.144	3.580

	Mean	Stdev
rise-13-TTAA	3.413	0.297

	Mean	Stdev
roll-13-TTAA	0.237	5.106

	Mean	Stdev
shift-13-TTAA	0.000	0.565

	Mean	Stdev
slide-13-TTAA	-0.500	0.483

	Mean	Stdev
tilt-13-TTAA	0.000	4.904

	Mean	Stdev
twist-13-TTAA	37.932	4.029

	Mean	Stdev
rise-14-TTAA	3.426	0.328

	Mean	Stdev
roll-14-TTAA	2.045	6.227

	Mean	Stdev
shift-14-TTAA	0.000	0.881

	Mean	Stdev
slide-14-TTAA	-0.057	0.486

	Mean	Stdev
tilt-14-TTAA	0.000	5.192

	Mean	Stdev
twist-14-TTAA	37.300	5.814

	Mean	Stdev
rise-2-AGCT	3.506	0.345

	Mean	Stdev
roll-2-AGCT	4.525	5.773

	Mean	Stdev
shift-2-AGCT	0.000	0.833

	Mean	Stdev
slide-2-AGCT	-0.547	0.543

	Mean	Stdev
tilt-2-AGCT	0.000	4.896

	Mean	Stdev
twist-2-AGCT	34.373	5.249

	Mean	Stdev
rise-3-GATC	3.481	0.295

	Mean	Stdev
roll-3-GATC	0.084	5.459

	Mean	Stdev
shift-3-GATC	0.000	0.679

	Mean	Stdev
slide-3-GATC	-0.400	0.623

	Mean	Stdev
tilt-3-GATC	0.000	4.384

	Mean	Stdev
twist-3-GATC	39.739	4.530

	Mean	Stdev
rise-4-ATAT	3.215	0.255

	Mean	Stdev
roll-4-ATAT	-1.678	5.082

	Mean	Stdev
shift-4-ATAT	0.000	0.619

	Mean	Stdev
slide-4-ATAT	-0.775	0.371

	Mean	Stdev
tilt-4-ATAT	0.000	3.573

	Mean	Stdev
twist-4-ATAT	30.536	4.092

	Mean	Stdev
rise-5-TATA	3.208	0.315

	Mean	Stdev
roll-5-TATA	4.909	7.628

	Mean	Stdev
shift-5-TATA	0.000	1.403

	Mean	Stdev
slide-5-TATA	0.149	0.621

	Mean	Stdev
tilt-5-TATA	0.000	5.712

	Mean	Stdev
twist-5-TATA	34.798	6.690

	Mean	Stdev
rise-6-AATT	3.240	0.257

	Mean	Stdev
roll-6-AATT	0.662	5.499

	Mean	Stdev
shift-6-AATT	0.000	1.163

	Mean	Stdev
slide-6-AATT	0.079	0.498

	Mean	Stdev
tilt-6-AATT	0.000	5.543

	Mean	Stdev
twist-6-AATT	36.432	4.388

	Mean	Stdev
rise-7-ACGT	3.418	0.282

	Mean	Stdev
roll-7-ACGT	-1.244	5.153

	Mean	Stdev
shift-7-ACGT	0.000	0.675

	Mean	Stdev
slide-7-ACGT	-0.731	0.448

	Mean	Stdev
tilt-7-ACGT	0.000	3.979

	Mean	Stdev
twist-7-ACGT	33.253	4.385

	Mean	Stdev
rise-8-CGCG	3.314	0.333

	Mean	Stdev
roll-8-CGCG	6.747	6.506

	Mean	Stdev
shift-8-CGCG	0.000	1.122

	Mean	Stdev
slide-8-CGCG	0.158	0.518

	Mean	Stdev
tilt-8-CGCG	0.000	6.625

	Mean	Stdev
twist-8-CGCG	35.887	5.721

	Mean	Stdev
rise-9-GATC	3.314	0.285

	Mean	Stdev
roll-9-GATC	1.348	5.939

	Mean	Stdev
shift-9-GATC	0.000	1.109

	Mean	Stdev
slide-9-GATC	0.076	0.616

	Mean	Stdev
tilt-9-GATC	0.000	5.736

	Mean	Stdev
twist-9-GATC	36.693	4.958

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