NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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5'	A	-	A	-	G	-	T	-	A	-	C	-	A	-	A	-	A	-	C	-	T	-	T	-	T	-	C	-	T	-	T	-	G	-	T	-	A	-	T	3'
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3'	T	-	T	-	C	-	A	-	T	-	G	-	T	-	T	-	T	-	G	-	A	-	A	-	A	-	G	-	A	-	A	-	C	-	A	-	T	-	A	5'
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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.579	0.643	0.509	-0.060	0.016	0.048
Slide	0.643	2.728	1.183	-0.010	-0.082	-0.135
Rise	0.509	1.183	9.546	-0.260	-0.031	-0.233
Tilt	-0.060	-0.010	-0.260	0.047	0.000	0.009
Roll	0.016	-0.082	-0.031	0.000	0.024	0.012
Twist	0.048	-0.135	-0.233	0.009	0.012	0.035

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.856	0.615	0.633	-0.042	0.014	0.064
Slide	0.615	3.433	1.437	-0.019	-0.045	-0.185
Rise	0.633	1.437	9.523	-0.266	-0.032	-0.174
Tilt	-0.042	-0.019	-0.266	0.048	-0.000	0.008
Roll	0.014	-0.045	-0.032	-0.000	0.022	0.005
Twist	0.064	-0.185	-0.174	0.008	0.005	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.147	0.114	0.479	-0.024	0.033	0.020
Slide	0.114	3.884	3.266	-0.045	0.017	-0.090
Rise	0.479	3.266	11.756	-0.041	0.109	-0.241
Tilt	-0.024	-0.045	-0.041	0.038	0.004	0.002
Roll	0.033	0.017	0.109	0.004	0.024	0.005
Twist	0.020	-0.090	-0.241	0.002	0.005	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.070	0.267	0.278	-0.051	0.004	0.049
Slide	0.267	4.031	2.854	-0.019	0.025	-0.192
Rise	0.278	2.854	10.900	0.052	0.104	-0.175
Tilt	-0.051	-0.019	0.052	0.049	0.004	0.004
Roll	0.004	0.025	0.104	0.004	0.030	0.005
Twist	0.049	-0.192	-0.175	0.004	0.005	0.053

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.251	0.616	-0.236	0.002	0.027	0.022
Slide	0.616	2.833	0.987	0.004	-0.031	-0.119
Rise	-0.236	0.987	2.633	0.099	-0.039	-0.137
Tilt	0.002	0.004	0.099	0.021	0.002	0.007
Roll	0.027	-0.031	-0.039	0.002	0.017	0.001
Twist	0.022	-0.119	-0.137	0.007	0.001	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.195	-0.069	0.154	-0.063	-0.007	-0.028
Slide	-0.069	2.354	1.001	0.019	-0.009	-0.108
Rise	0.154	1.001	6.382	-0.014	-0.067	-0.179
Tilt	-0.063	0.019	-0.014	0.026	0.000	0.002
Roll	-0.007	-0.009	-0.067	0.000	0.014	0.005
Twist	-0.028	-0.108	-0.179	0.002	0.005	0.023

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	2.122	-0.520	-0.620	-0.074	-0.040	-0.084
Slide	-0.520	3.659	1.494	-0.035	-0.053	-0.223
Rise	-0.620	1.494	7.717	0.159	-0.037	-0.129
Tilt	-0.074	-0.035	0.159	0.042	-0.002	-0.004
Roll	-0.040	-0.053	-0.037	-0.002	0.025	0.004
Twist	-0.084	-0.223	-0.129	-0.004	0.004	0.050

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.167	0.067	-0.307	-0.063	-0.053	-0.093
Slide	0.067	2.079	1.759	0.020	-0.051	-0.120
Rise	-0.307	1.759	8.012	0.227	-0.058	-0.216
Tilt	-0.063	0.020	0.227	0.043	-0.004	-0.008
Roll	-0.053	-0.051	-0.058	-0.004	0.021	0.009
Twist	-0.093	-0.120	-0.216	-0.008	0.009	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.155	0.278	-0.241	-0.015	-0.032	-0.026
Slide	0.278	3.962	3.375	0.054	0.039	-0.075
Rise	-0.241	3.376	10.745	-0.003	0.121	-0.185
Tilt	-0.015	0.054	-0.003	0.042	-0.005	-0.008
Roll	-0.032	0.039	0.121	-0.005	0.023	0.006
Twist	-0.026	-0.075	-0.185	-0.008	0.006	0.035

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.367	-0.274	-0.149	-0.043	0.013	-0.056
Slide	-0.274	4.155	3.391	-0.010	0.024	-0.088
Rise	-0.149	3.391	11.506	0.003	0.080	-0.246
Tilt	-0.043	-0.010	0.003	0.044	-0.005	-0.002
Roll	0.013	0.024	0.080	-0.005	0.026	0.003
Twist	-0.056	-0.088	-0.246	-0.002	0.003	0.029

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.707	-0.323	0.376	-0.050	-0.004	0.001
Slide	-0.323	3.232	0.456	-0.033	0.010	-0.163
Rise	0.376	0.456	7.898	-0.054	-0.084	-0.161
Tilt	-0.050	-0.033	-0.054	0.026	-0.003	-0.003
Roll	-0.004	0.010	-0.084	-0.003	0.015	0.007
Twist	0.001	-0.163	-0.161	-0.003	0.007	0.032

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.545	-0.093	-0.001	-0.065	0.001	0.001
Slide	-0.093	2.393	0.362	0.001	-0.012	-0.171
Rise	-0.001	0.362	9.317	0.002	-0.132	-0.217
Tilt	-0.065	0.001	0.002	0.026	-0.000	0.002
Roll	0.001	-0.012	-0.132	-0.000	0.019	0.014
Twist	0.001	-0.171	-0.217	0.002	0.014	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.242	-0.269	-0.967	-0.096	-0.037	-0.011
Slide	-0.269	2.000	1.524	0.001	-0.024	-0.107
Rise	-0.967	1.524	9.365	0.310	-0.001	-0.168
Tilt	-0.096	0.001	0.310	0.043	0.004	-0.001
Roll	-0.037	-0.024	-0.001	0.004	0.021	0.010
Twist	-0.011	-0.107	-0.168	-0.001	0.010	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.892	0.090	-0.262	-0.089	0.002	0.029
Slide	0.090	2.323	1.168	-0.002	0.007	-0.104
Rise	-0.262	1.168	6.683	0.002	-0.068	-0.206
Tilt	-0.089	-0.002	0.002	0.029	0.002	-0.003
Roll	0.002	0.007	-0.068	0.002	0.015	0.007
Twist	0.029	-0.104	-0.206	-0.003	0.007	0.022

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.842	-0.225	-0.557	-0.031	-0.005	-0.071
Slide	-0.225	2.790	0.879	-0.022	-0.083	-0.134
Rise	-0.557	0.879	9.100	0.225	-0.043	-0.170
Tilt	-0.031	-0.022	0.225	0.044	0.001	-0.007
Roll	-0.005	-0.083	-0.043	0.001	0.023	0.011
Twist	-0.071	-0.134	-0.170	-0.007	0.011	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.519	-0.414	-0.341	-0.020	-0.015	-0.036
Slide	-0.414	2.631	1.339	0.000	-0.091	-0.129
Rise	-0.341	1.339	9.070	0.245	-0.035	-0.206
Tilt	-0.020	0.000	0.245	0.042	0.000	-0.006
Roll	-0.015	-0.091	-0.035	0.000	0.023	0.008
Twist	-0.036	-0.129	-0.206	-0.006	0.008	0.030

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.488	-0.502	-0.647	-0.045	-0.012	-0.059
Slide	-0.502	2.657	1.285	0.018	-0.082	-0.139
Rise	-0.647	1.285	10.122	0.263	-0.036	-0.215
Tilt	-0.045	0.018	0.263	0.046	0.001	-0.008
Roll	-0.012	-0.082	-0.036	0.001	0.023	0.012
Twist	-0.059	-0.139	-0.215	-0.008	0.012	0.037

[Image courtesy of Curves+]

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	Mean	Stdev
-aatt.7.cte	0.000	0.000

	Mean	Stdev
-aatt.8.cte	0.000	0.000

	Mean	Stdev
-acgt.5.cte	0.000	0.000

	Mean	Stdev
-acgt.9.cte	0.000	0.000

	Mean	Stdev
-agct.2.cte	0.000	0.000

	Mean	Stdev
-catg.6.cte	0.000	0.000

	Mean	Stdev
-ctag.10.cte	0.000	0.000

	Mean	Stdev
-ctag.14.cte	0.000	0.000

	Mean	Stdev
-gtac.17.cte	0.000	0.000

	Mean	Stdev
-gtac.3.cte	0.000	0.000

	Mean	Stdev
-tata.18.cte	0.000	0.000

	Mean	Stdev
-tata.4.cte	0.000	0.000

	Mean	Stdev
-tcga.13.cte	0.000	0.000

	Mean	Stdev
-tgca.16.cte	0.000	0.000

	Mean	Stdev
-ttaa.11.cte	0.000	0.000

	Mean	Stdev
-ttaa.12.cte	0.000	0.000

	Mean	Stdev
-ttaa.15.cte	0.000	0.000

	Mean	Stdev
rise-10-CTAG	3.495	0.303

	Mean	Stdev
roll-10-CTAG	2.298	5.116

	Mean	Stdev
shift-10-CTAG	0.343	0.666

	Mean	Stdev
slide-10-CTAG	-0.946	0.568

	Mean	Stdev
tilt-10-CTAG	2.301	4.318

	Mean	Stdev
twist-10-CTAG	35.198	4.753

	Mean	Stdev
rise-11-TTAA	3.244	0.287

	Mean	Stdev
roll-11-TTAA	0.317	5.519

	Mean	Stdev
shift-11-TTAA	0.030	0.627

	Mean	Stdev
slide-11-TTAA	-0.558	0.540

	Mean	Stdev
tilt-11-TTAA	2.258	4.053

	Mean	Stdev
twist-11-TTAA	34.223	5.129

	Mean	Stdev
rise-12-TTAA	3.275	0.303

	Mean	Stdev
roll-12-TTAA	1.138	5.525

	Mean	Stdev
shift-12-TTAA	0.161	0.681

	Mean	Stdev
slide-12-TTAA	-0.507	0.595

	Mean	Stdev
tilt-12-TTAA	1.598	4.160

	Mean	Stdev
twist-12-TTAA	32.716	5.621

	Mean	Stdev
rise-13-TCGA	3.421	0.322

	Mean	Stdev
roll-13-TCGA	1.589	5.904

	Mean	Stdev
shift-13-TCGA	0.077	0.793

	Mean	Stdev
slide-13-TCGA	-0.358	0.646

	Mean	Stdev
tilt-13-TCGA	-0.995	4.671

	Mean	Stdev
twist-13-TCGA	36.370	5.559

	Mean	Stdev
rise-14-CTAG	3.437	0.338

	Mean	Stdev
roll-14-CTAG	3.081	6.055

	Mean	Stdev
shift-14-CTAG	0.229	0.961

	Mean	Stdev
slide-14-CTAG	-0.616	0.647

	Mean	Stdev
tilt-14-CTAG	1.854	4.480

	Mean	Stdev
twist-14-CTAG	34.355	5.671

	Mean	Stdev
rise-15-TTAA	3.200	0.282

	Mean	Stdev
roll-15-TTAA	2.266	5.620

	Mean	Stdev
shift-15-TTAA	0.243	0.753

	Mean	Stdev
slide-15-TTAA	-0.231	0.592

	Mean	Stdev
tilt-15-TTAA	3.088	3.950

	Mean	Stdev
twist-15-TTAA	33.070	5.508

	Mean	Stdev
rise-16-TGCA	3.315	0.353

	Mean	Stdev
roll-16-TGCA	7.034	7.153

	Mean	Stdev
shift-16-TGCA	0.467	1.011

	Mean	Stdev
slide-16-TGCA	-0.168	0.602

	Mean	Stdev
tilt-16-TGCA	-0.341	5.473

	Mean	Stdev
twist-16-TGCA	33.028	7.482

	Mean	Stdev
rise-17-GTAC	3.250	0.298

	Mean	Stdev
roll-17-GTAC	-2.052	5.564

	Mean	Stdev
shift-17-GTAC	-0.727	0.755

	Mean	Stdev
slide-17-GTAC	-0.553	0.467

	Mean	Stdev
tilt-17-GTAC	0.106	3.963

	Mean	Stdev
twist-17-GTAC	31.629	4.608

	Mean	Stdev
rise-18-TATA	3.193	0.299

	Mean	Stdev
roll-18-TATA	3.703	7.043

	Mean	Stdev
shift-18-TATA	1.151	1.048

	Mean	Stdev
slide-18-TATA	0.436	0.551

	Mean	Stdev
tilt-18-TATA	4.185	5.460

	Mean	Stdev
twist-18-TATA	37.924	5.797

	Mean	Stdev
rise-2-AGCT	3.719	0.769

	Mean	Stdev
roll-2-AGCT	3.443	6.525

	Mean	Stdev
shift-2-AGCT	-0.413	0.792

	Mean	Stdev
slide-2-AGCT	-0.789	0.578

	Mean	Stdev
tilt-2-AGCT	-5.335	7.718

	Mean	Stdev
twist-2-AGCT	37.866	6.733

	Mean	Stdev
rise-3-GTAC	3.380	0.285

	Mean	Stdev
roll-3-GTAC	-2.042	4.944

	Mean	Stdev
shift-3-GTAC	-0.252	0.722

	Mean	Stdev
slide-3-GTAC	-0.727	0.437

	Mean	Stdev
tilt-3-GTAC	1.288	3.752

	Mean	Stdev
twist-3-GTAC	31.746	5.446

	Mean	Stdev
rise-4-TATA	3.167	0.274

	Mean	Stdev
roll-4-TATA	4.966	6.936

	Mean	Stdev
shift-4-TATA	0.046	1.248

	Mean	Stdev
slide-4-TATA	0.319	0.631

	Mean	Stdev
tilt-4-TATA	-0.052	5.739

	Mean	Stdev
twist-4-TATA	36.060	5.824

	Mean	Stdev
rise-5-ACGT	3.206	0.285

	Mean	Stdev
roll-5-ACGT	-2.575	5.371

	Mean	Stdev
shift-5-ACGT	0.706	0.746

	Mean	Stdev
slide-5-ACGT	-0.618	0.448

	Mean	Stdev
tilt-5-ACGT	-0.984	4.049

	Mean	Stdev
twist-5-ACGT	29.510	5.164

	Mean	Stdev
rise-6-CATG	3.320	0.346

	Mean	Stdev
roll-6-CATG	6.758	6.768

	Mean	Stdev
shift-6-CATG	-0.488	0.763

	Mean	Stdev
slide-6-CATG	-0.443	0.577

	Mean	Stdev
tilt-6-CATG	2.138	5.156

	Mean	Stdev
twist-6-CATG	31.323	6.681

	Mean	Stdev
rise-7-AATT	3.259	0.293

	Mean	Stdev
roll-7-AATT	2.903	5.543

	Mean	Stdev
shift-7-AATT	-0.559	0.750

	Mean	Stdev
slide-7-AATT	-0.025	0.601

	Mean	Stdev
tilt-7-AATT	-2.995	4.038

	Mean	Stdev
twist-7-AATT	34.130	5.750

	Mean	Stdev
rise-8-AATT	3.336	0.284

	Mean	Stdev
roll-8-AATT	0.482	5.280

	Mean	Stdev
shift-8-AATT	-0.169	0.664

	Mean	Stdev
slide-8-AATT	-0.421	0.538

	Mean	Stdev
tilt-8-AATT	-2.744	3.918

	Mean	Stdev
twist-8-AATT	36.011	5.158

	Mean	Stdev
rise-9-ACGT	3.474	0.263

	Mean	Stdev
roll-9-ACGT	-0.748	4.601

	Mean	Stdev
shift-9-ACGT	0.074	0.555

	Mean	Stdev
slide-9-ACGT	-1.015	0.459

	Mean	Stdev
tilt-9-ACGT	-1.458	3.578

	Mean	Stdev
twist-9-ACGT	34.179	3.810

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	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20
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3'	T	-	T	-	C	-	A	-	T	-	G	-	T	-	T	-	T	-	G	-	A	-	A	-	A	-	G	-	A	-	A	-	C	-	A	-	T	-	A	5'
	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21

	1		2		3		4		5		6		7		8		9		10		11		12		13		14		15		16		17		18		19		20
5'	A	-	A	-	G	-	T	-	A	-	C	-	A	-	A	-	A	-	C	-	T	-	T	-	T	-	C	-	T	-	T	-	G	-	T	-	A	-	T	3'
			\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|	x	\|
3'	T	-	T	-	C	-	A	-	T	-	G	-	T	-	T	-	T	-	G	-	A	-	A	-	A	-	G	-	A	-	A	-	C	-	A	-	T	-	A	5'
	40		39		38		37		36		35		34		33		32		31		30		29		28		27		26		25		24		23		22		21