NAFlex. Nucleic Acids Flexibility

Nucleic Acid Analysis - Stiffness Analysis

(Using Curves+ program)

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Interactive Sequence (see Help)

Average Results

Results by Time

Stiffness by Helical Parameters

Stiffness By Base Pair Step

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

Shift

Slide

Rise

Tilt

Roll

Twist

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.642	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	2.456	-0.288	0.000	0.015	-0.036
Rise	0.000	-0.288	8.407	0.000	-0.154	-0.228
Tilt	-0.067	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.015	-0.154	0.000	0.024	0.007
Twist	0.000	-0.036	-0.228	0.000	0.007	0.017

[Image courtesy of Curves+]

Units:
Diagonal Shift/Slide/Rise in kcal/(mol*Å²), Diagonal Tilt/Roll/Twist in kcal/(mol*degree²)
Out of Diagonal: Shift/Slide/Rise in kcal/(mol*Å), Out of Diagonal Tilt/Roll/Twist in kcal/(mol*degree)

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.441	0.000	0.000	-0.015	0.000	0.000
Slide	0.000	1.089	0.570	0.000	-0.009	-0.002
Rise	0.000	0.570	4.563	0.000	-0.050	-0.085
Tilt	-0.015	0.000	0.000	0.015	0.000	0.000
Roll	0.000	-0.009	-0.050	0.000	0.007	0.000
Twist	0.000	-0.002	-0.085	0.000	0.000	0.009

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.566	0.000	0.000	-0.067	0.000	0.000
Slide	0.000	2.787	0.111	0.000	0.004	-0.066
Rise	0.000	0.111	8.817	0.000	-0.156	-0.259
Tilt	-0.067	0.000	0.000	0.026	0.000	0.000
Roll	0.000	0.004	-0.156	0.000	0.024	0.008
Twist	0.000	-0.066	-0.259	0.000	0.008	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.715	0.000	0.000	-0.065	0.000	0.000
Slide	0.000	2.596	-0.217	0.000	0.012	-0.043
Rise	0.000	-0.217	8.774	0.000	-0.148	-0.249
Tilt	-0.065	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.012	-0.148	0.000	0.023	0.007
Twist	0.000	-0.043	-0.249	0.000	0.007	0.019

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.979	0.000	0.000	-0.096	0.000	0.000
Slide	0.000	2.159	1.602	0.000	0.003	-0.208
Rise	0.000	1.602	7.960	0.000	-0.018	-0.173
Tilt	-0.096	0.000	0.000	0.040	0.000	0.000
Roll	0.000	0.003	-0.018	0.000	0.022	0.008
Twist	0.000	-0.208	-0.173	0.000	0.008	0.042

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.010	0.000	0.000	-0.092	0.000	0.000
Slide	0.000	2.143	1.628	0.000	-0.004	-0.224
Rise	0.000	1.628	7.878	0.000	-0.024	-0.192
Tilt	-0.092	0.000	0.000	0.038	0.000	0.000
Roll	0.000	-0.004	-0.024	0.000	0.023	0.009
Twist	0.000	-0.224	-0.192	0.000	0.009	0.046

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.007	0.000	0.000	-0.094	0.000	0.000
Slide	0.000	2.270	1.618	0.000	0.004	-0.222
Rise	0.000	1.618	7.905	0.000	-0.027	-0.165
Tilt	-0.094	0.000	0.000	0.039	0.000	0.000
Roll	0.000	0.004	-0.027	0.000	0.022	0.008
Twist	0.000	-0.222	-0.165	0.000	0.008	0.043

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.523	0.000	0.000	-0.023	0.000	0.000
Slide	0.000	4.542	2.779	0.000	-0.013	-0.185
Rise	0.000	2.779	9.802	0.000	0.062	-0.183
Tilt	-0.023	0.000	0.000	0.042	0.000	0.000
Roll	0.000	-0.013	0.062	0.000	0.031	0.010
Twist	0.000	-0.185	-0.183	0.000	0.010	0.052

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.250	0.000	0.000	-0.053	0.000	0.000
Slide	0.000	3.640	2.599	0.000	0.031	-0.166
Rise	0.000	2.599	9.417	0.000	0.096	-0.210
Tilt	-0.053	0.000	0.000	0.037	0.000	0.000
Roll	0.000	0.031	0.096	0.000	0.024	0.010
Twist	0.000	-0.166	-0.210	0.000	0.010	0.043

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.144	0.000	0.000	-0.055	0.000	0.000
Slide	0.000	3.788	2.764	0.000	0.013	-0.102
Rise	0.000	2.764	9.872	0.000	0.067	-0.210
Tilt	-0.055	0.000	0.000	0.041	0.000	0.000
Roll	0.000	0.013	0.067	0.000	0.029	0.013
Twist	0.000	-0.102	-0.210	0.000	0.013	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	1.527	0.000	0.000	-0.026	0.000	0.000
Slide	0.000	4.638	3.004	0.000	-0.003	-0.178
Rise	0.000	3.004	9.714	0.000	0.074	-0.177
Tilt	-0.026	0.000	0.000	0.044	0.000	0.000
Roll	0.000	-0.003	0.074	0.000	0.030	0.009
Twist	0.000	-0.178	-0.177	0.000	0.009	0.049

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.930	0.000	0.000	-0.090	0.000	0.000
Slide	0.000	2.778	1.252	0.000	-0.054	-0.181
Rise	0.000	1.252	8.079	0.000	-0.024	-0.160
Tilt	-0.090	0.000	0.000	0.034	0.000	0.000
Roll	0.000	-0.054	-0.024	0.000	0.023	0.012
Twist	0.000	-0.181	-0.160	0.000	0.012	0.040

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.965	0.000	0.000	-0.103	0.000	0.000
Slide	0.000	2.687	1.235	0.000	-0.051	-0.170
Rise	0.000	1.235	8.330	0.000	-0.030	-0.169
Tilt	-0.103	0.000	0.000	0.033	0.000	0.000
Roll	0.000	-0.051	-0.030	0.000	0.023	0.012
Twist	0.000	-0.170	-0.169	0.000	0.012	0.037

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.972	0.000	0.000	-0.097	0.000	0.000
Slide	0.000	2.889	1.218	0.000	-0.057	-0.190
Rise	0.000	1.219	8.336	0.000	-0.027	-0.168
Tilt	-0.097	0.000	0.000	0.036	0.000	0.000
Roll	0.000	-0.057	-0.027	0.000	0.023	0.012
Twist	0.000	-0.190	-0.168	0.000	0.012	0.041

[Image courtesy of Curves+]

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	Shift	Slide	Rise	Tilt	Roll	Twist
Shift	0.966	0.000	0.000	-0.036	0.000	0.000
Slide	0.000	1.707	0.910	0.000	0.008	-0.049
Rise	0.000	0.910	5.958	0.000	-0.069	-0.171
Tilt	-0.036	0.000	0.000	0.027	0.000	0.000
Roll	0.000	0.008	-0.069	0.000	0.017	0.007
Twist	0.000	-0.049	-0.171	0.000	0.007	0.017

[Image courtesy of Curves+]

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	Mean	Stdev
-cgcg.13.cte	0.000	0.000

	Mean	Stdev
-cgcg.2.cte	0.000	0.000

	Mean	Stdev
-cgcg.5.cte	0.000	0.000

	Mean	Stdev
-cgcg.9.cte	0.000	0.000

	Mean	Stdev
-ggcc.10.cte	0.000	0.000

	Mean	Stdev
-ggcc.14.cte	0.000	0.000

	Mean	Stdev
-ggcc.6.cte	0.000	0.000

	Mean	Stdev
-gtac.11.cte	0.000	0.000

	Mean	Stdev
-gtac.15.cte	0.000	0.000

	Mean	Stdev
-gtac.3.cte	0.000	0.000

	Mean	Stdev
-gtac.7.cte	0.000	0.000

	Mean	Stdev
-tcga.12.cte	0.000	0.000

	Mean	Stdev
-tcga.4.cte	0.000	0.000

	Mean	Stdev
-tcga.8.cte	0.000	0.000

	Mean	Stdev
-tgca.16.cte	0.000	0.000

	Mean	Stdev
rise-10-GGCC	3.619	0.295

	Mean	Stdev
roll-10-GGCC	3.503	5.642

	Mean	Stdev
shift-10-GGCC	0.000	0.885

	Mean	Stdev
slide-10-GGCC	-0.485	0.783

	Mean	Stdev
tilt-10-GGCC	0.000	4.372

	Mean	Stdev
twist-10-GGCC	36.439	5.651

	Mean	Stdev
rise-11-GTAC	3.433	0.275

	Mean	Stdev
roll-11-GTAC	-0.356	4.605

	Mean	Stdev
shift-11-GTAC	0.000	0.623

	Mean	Stdev
slide-11-GTAC	-0.755	0.416

	Mean	Stdev
tilt-11-GTAC	0.000	3.749

	Mean	Stdev
twist-11-GTAC	32.733	3.823

	Mean	Stdev
rise-12-TCGA	3.398	0.284

	Mean	Stdev
roll-12-TCGA	0.513	5.446

	Mean	Stdev
shift-12-TCGA	0.000	0.918

	Mean	Stdev
slide-12-TCGA	0.203	0.552

	Mean	Stdev
tilt-12-TCGA	0.000	4.790

	Mean	Stdev
twist-12-TCGA	38.900	4.919

	Mean	Stdev
rise-13-CGCG	2.879	0.342

	Mean	Stdev
roll-13-CGCG	10.243	5.352

	Mean	Stdev
shift-13-CGCG	0.000	0.631

	Mean	Stdev
slide-13-CGCG	-0.036	0.510

	Mean	Stdev
tilt-13-CGCG	0.000	5.034

	Mean	Stdev
twist-13-CGCG	22.792	7.671

	Mean	Stdev
rise-14-GGCC	3.580	0.298

	Mean	Stdev
roll-14-GGCC	3.247	5.531

	Mean	Stdev
shift-14-GGCC	0.000	0.862

	Mean	Stdev
slide-14-GGCC	-0.476	0.803

	Mean	Stdev
tilt-14-GGCC	0.000	4.419

	Mean	Stdev
twist-14-GGCC	36.369	5.523

	Mean	Stdev
rise-15-GTAC	3.403	0.293

	Mean	Stdev
roll-15-GTAC	-0.431	5.568

	Mean	Stdev
shift-15-GTAC	0.000	0.707

	Mean	Stdev
slide-15-GTAC	-0.646	0.480

	Mean	Stdev
tilt-15-GTAC	0.000	4.123

	Mean	Stdev
twist-15-GTAC	32.969	4.564

	Mean	Stdev
rise-16-TGCA	3.308	0.377

	Mean	Stdev
roll-16-TGCA	8.875	6.583

	Mean	Stdev
shift-16-TGCA	0.000	0.799

	Mean	Stdev
slide-16-TGCA	-0.292	0.631

	Mean	Stdev
tilt-16-TGCA	0.000	4.825

	Mean	Stdev
twist-16-TGCA	32.740	7.527

	Mean	Stdev
rise-2-CGCG	3.491	0.428

	Mean	Stdev
roll-2-CGCG	7.564	9.524

	Mean	Stdev
shift-2-CGCG	0.000	1.172

	Mean	Stdev
slide-2-CGCG	-0.114	0.763

	Mean	Stdev
tilt-2-CGCG	0.000	6.278

	Mean	Stdev
twist-2-CGCG	36.450	9.121

	Mean	Stdev
rise-3-GTAC	3.366	0.291

	Mean	Stdev
roll-3-GTAC	-0.343	5.083

	Mean	Stdev
shift-3-GTAC	0.000	0.740

	Mean	Stdev
slide-3-GTAC	-0.617	0.445

	Mean	Stdev
tilt-3-GTAC	0.000	3.899

	Mean	Stdev
twist-3-GTAC	31.306	4.575

	Mean	Stdev
rise-4-TCGA	3.362	0.281

	Mean	Stdev
roll-4-TCGA	0.223	5.570

	Mean	Stdev
shift-4-TCGA	0.000	0.957

	Mean	Stdev
slide-4-TCGA	0.240	0.558

	Mean	Stdev
tilt-4-TCGA	0.000	5.210

	Mean	Stdev
twist-4-TCGA	38.843	5.141

	Mean	Stdev
rise-5-CGCG	2.880	0.341

	Mean	Stdev
roll-5-CGCG	10.165	5.458

	Mean	Stdev
shift-5-CGCG	0.000	0.648

	Mean	Stdev
slide-5-CGCG	-0.053	0.492

	Mean	Stdev
tilt-5-CGCG	0.000	4.993

	Mean	Stdev
twist-5-CGCG	23.017	7.846

	Mean	Stdev
rise-6-GGCC	3.638	0.296

	Mean	Stdev
roll-6-GGCC	3.841	5.620

	Mean	Stdev
shift-6-GGCC	0.000	0.867

	Mean	Stdev
slide-6-GGCC	-0.559	0.784

	Mean	Stdev
tilt-6-GGCC	0.000	4.397

	Mean	Stdev
twist-6-GGCC	36.157	5.642

	Mean	Stdev
rise-7-GTAC	3.426	0.282

	Mean	Stdev
roll-7-GTAC	-0.371	4.642

	Mean	Stdev
shift-7-GTAC	0.000	0.623

	Mean	Stdev
slide-7-GTAC	-0.757	0.417

	Mean	Stdev
tilt-7-GTAC	0.000	3.678

	Mean	Stdev
twist-7-GTAC	32.759	3.919

	Mean	Stdev
rise-8-TCGA	3.398	0.279

	Mean	Stdev
roll-8-TCGA	0.438	5.461

	Mean	Stdev
shift-8-TCGA	0.000	0.905

	Mean	Stdev
slide-8-TCGA	0.182	0.545

	Mean	Stdev
tilt-8-TCGA	0.000	4.674

	Mean	Stdev
twist-8-TCGA	38.798	4.950

	Mean	Stdev
rise-9-CGCG	2.861	0.340

	Mean	Stdev
roll-9-CGCG	10.254	5.429

	Mean	Stdev
shift-9-CGCG	0.000	0.614

	Mean	Stdev
slide-9-CGCG	-0.061	0.498

	Mean	Stdev
tilt-9-CGCG	0.000	4.921

	Mean	Stdev
twist-9-CGCG	22.641	7.568

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