Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines.
|Title||Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines.|
|Publication Type||Journal Article|
|Year of Publication||2002|
|Authors||Aviñó, Anna, Frieden Miriam, Morales Juan Carlos, de la Torre Beatriz García, García Ramón Güimil, Azorín Ferran, Gelpí Josep-Lluis, Orozco Modesto, González Carlos, and Eritja Ramon|
|Journal||Nucleic Acids Res|
|Date Published||2002 Jun 15|
|Keywords||Amines, Base Sequence, Biomolecular, Circular Dichroism, DNA, Electrophoretic Mobility Shift Assay, Guanine, Hot Temperature, Models, Molecular, Nuclear Magnetic Resonance, Nucleic Acid Conformation, Nucleic Acid Denaturation, Oligonucleotides, Purines, Pyrimidines, Salts|
Parallel-stranded hairpins with a polypyrimidine sequence linked to a complementary purine carrying 8-aminopurines such as 8-aminoadenine, 8-aminoguanine and 8-aminohypoxanthine bind polypyrimidine sequences complementary (in an antiparallel sense) to the purine part by a triple helix. The relative stabilities of triplexes were assessed by UV-absorption melting experiments as a function of pH and salt concentration. Hairpins carrying 8-aminopurines give very stable triple helical structures even at neutral pH, as confirmed by gel-shift experiments, circular dichroism and nuclear magnetic resonance spectroscopy. The modified hairpins may be redesigned to cope with small interruptions in the polypyrimidine target sequence.