DNA-triplex stabilizing properties of 8-aminoguanine

TitleDNA-triplex stabilizing properties of 8-aminoguanine
Publication TypeJournal Article
Year of Publication2000
AuthorsSoliva, Robert, García Ramón Güimil, J. Blas Ramón, Eritja Ramon, Asensio Juan Luis, González Carlos, F. Luque Javier, and Orozco Modesto
JournalNucleic Acids Research
Volume28
Pagination4531-4539
Abstract

A DNA-triplex stabilizing purine (8-aminoguanine) is designed based on molecular modeling and synthesized. The substitution of guanine by 8-aminoguanine largely stabilizes the triplex both at neutral and acidic pH, as suggested by molecular dynamics and thermodynamic integration calculations, and demonstrated by melting experiments. NMR experiments confirm the triplex-stabilizing properties of 8-aminoguanine and demonstrate that few changes are found in the structure of the triplex due to the presence of this modified base.

URLhttp://nar.oxfordjournals.org/content/28/22/4531.abstract
DOI10.1093/nar/28.22.4531
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